• Mahfud . Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan Universitas Muhammadiyah Kupang
  • Adi Winarto Bagian Anatomi, Histologi dan Embriologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
  • Chairun Nisa Bagian Anatomi, Histologi dan Embriologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
Keywords: Varanus salvator bivittatus, bulbourethral gland, morphology


The study was aimed to describe the macromorphological and micromorphological aspect of the accessories gland of Male Varanus salvator bivittatus. Two adult male lizards with 45.60 cm SVL were used in this experiment. The animals were sacrificed by exsanguination under deep anesthetized and fixed in 4 % paraformaldehyde through perfusion and then its visceral site was observed. Histomorphological evaluation was obtained by paraffin preparation with section thickness of 3-4 μm then stained in Hematoxylin-Eosin (HE), Alcian Blue (AB) pH 2.5 and Periodic Acid Schiff (PAS). The result showed that accessories gland which was found at the dorsal of cloaca, specifically at the caudal end of deferent duct that was a hemipenile bulge. Microscopically, the accessories gland was identified as tubular mucous type gland which was resemble to bulbourethral gland in mammals. The cytoplasm of the secretory cells showed weak reaction, while strong reaction was observed in the luminal mucus which was stained by AB staining. In contrast, luminal mucus showed a weak reaction to PAS staining. In conclusion, V. salvator bivittatus has only one accessory gland and its microanatomical structure resembled the bulbourethral glands.


Aitken RNC. 1960. A histochemical study of the accessory genital glands of the boar. J Anat. 94: 130-142.

Bacha WJ Jr, Bacha LM. 2000. Color Atlas of Veterinary Histology, 2nd Edition. Philadelphia (US): Lippincott Williams & Wilkins.

Böhme W. 2003. Checklist of the living monitor lizards of the world (family Varanidae). Zoologische Verhandelingen Leiden 341(25):1-43.

Cabral SRP, Santos LR de Souza, Franco-Belussi L, Zieri R, Zago CES and DeOliveira C. 2011. Anatomy of the male reproductive system of Phrynops geoffroanus (Testudines: Chelidae). Maringá 33(4):487-492.

De Lisle HF. 2007. Observations on Varanus s. salvator in North Sulawesi. Biawak 1(2):59-66.

Del Canto R. 2007. Notes on the occurrence of Varanus auffenbergi on Roti Island. Biawak 1(1):24-25.

Desiani H, Mohamad K, Adnyane IKM, Agungpriyono S. 2000. Studi morfologi kelenjar aksesoris kelamin jantan tupai (tupaia glis) dengan tinjauan khusus pada sebaran karbohidrat. Media Veteriner. 7(4):6-10.

Eroschenko VP. 2008. Di Fiore's Atlas of Histology with Functional Correlations, 11th Edition. Philadelphia (US): Lippincott Williams & Wilkins.

Foss MA, Stewart N, Swift J. 2008. Cat Anatomy and Physiology. Rev. Edition. Washington State University (US). 4-H Cat Project, Unit 3, EM4289E. 4-H Youth Development Program.

Jenkins M dan Broad S. 1994. International trade in reptile skins: a review and analysis of the main consumer markets, 1983–1991. Cambridge. TRAFFIC International.

Karim SA. 1998. Macroscopic and microscopic anatomy of the hemipenes of the snake Bittis arietans arietans. JKAU: Sci 10:25-38.

Kiernan JA. 1990. Histological and Histochemiscal Method, 2nd Edition. England: Pergamon Pr.

Koch A, Auliya M, Schmitz A, Kuch U, Böhme W. 2007. Morphological studies on the systematics of South East Asian water monitors (Varanus salvator Complex): nominotypic populations and taxonomic overview. Mertensiella 16:109-180.

Mahfud, Nisa’ C, Winarto A. 2014. Anatomy of the male reproductive organ of water monitor, Varanus salvator bivittatus (Reptil: Varanidae), In: Proceedings The 3 Joint International Meeting 2014. Bogor, Indonesia, 13-15 Oct 2014. Pp:67-68.

Mardiastuti A, Soehartono T. 2003. Perdagangan Reptil Indonesia di Pasar Internasional. Di dalam: T. Harvey, Editor. Konservasi Amfibi dan Reptil di Indonesia. Prosiding Seminar Hasil Penelitian Departemen Konservasi Sumberdaya Hutan; 2003 Mei 8; Bogor, Indonesia. Bogor. Institut Pertanian Bogor. p131-144.

Mohamad K, Novelina S, Adnyane IKM, Agungpriyono S. 2001. Morfologi dan kandungan karbohidrat kelenjar aksesoris organ reproduksi tikus jantan pada umur sebelum dan setelah pubertas. Hayati. 8(4):91-97.

Porto M, de Oliveira MA, Pissinatti L, Rodrigues RL, Rojas-Moscoso JA, Cogo JC, Metze K, Antunes E, Nahoum C, Mónica FZ, de Nucci G. 2013. The Evolutionary Implications of Hemipenial Morphology of Rattlesnake Crotalus durissus terrificus (Laurent, 1768) (Serpentes: Viperidae: Crotalinae). PLoS ONE 8(6): 1-8.

Prades RB, Lastica EA and Acorda JA. 2013. Ultrasonography of the urogenital organs of male water monitor lizard (Varanus marmoratus, Weigmann, 1834). Philipp Journal Veterinary Animal Science 39(2):247-258.

Putra, YA, Masy’ud B, Ulfah, M. 2008. Keanekaragaman satwa berkhasiat obat di Taman Nasional Betung Kerihun, Kalimantan Barat, Indonesia. Med Konservasi. 13(1): 8-15.

Sever DM. 2004. Ultrastructure of the reproductive system of the black swamp snake (Seminatrix pygaea). IV. Occurrence of an ampulla ductus deferentis. Journal of Morpholpgy 262:714-730.

Shine R, Harlow PS, Keogh JS, Boeadi. 1996. Commercial harvesting of giant lizards: the biology of water monitors Varanus salvator in Southern Sumatra. Biologic Conservation 77:125-134.

Shine R, Ambariyanto, Harlow PS, Mumpuni. 1998. Ecological traits of commercially harvested water monitors, Varanus salvator, in Northern Sumatra. Wildlife Research 25:437-447.

Wahyuni S, Agungpriyono S, Agil M, Yusuf TL. 2012. Histologi dan histomorfometri testis dan epididimis Muncak (Muntiacus muntjak muntjak)pada periode ranggah keras. Jurnal Veteriner 13(3):211-219.
How to Cite
., M., Winarto, A., & Nisa, C. (2019). MORFOLOGI KELENJAR AKSESORIS KELAMIN BIAWAK AIR (Varanus salvator bivittatus) JANTAN. JURNAL KAJIAN VETERINER, 3(2), 83-91.

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.