Efek Pemaparan Deltamethrin pada Broiler Terhadap Aktivitas Enzim Alanin Aminotransferase, Aspartat Aminotransferase dan Gambaran Histopatologi Hepar

  • Nemay Anggadewi Ndaong Bagian Farmakologi dan Toksikologi Fakultas Kedokteran Hewan Universitas Nusa Cendana
  • Agustina Dwi Wijayanti Laboratorium Farmakologi Fakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta
  • Sitarina Widyarini Laboratorium Farmakologi Fakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta
Keywords: pyretroid, deltamethrin, AST, ALT, liverhistopathology, broiler

Abstract

The purpose of this study is to determine the effects of deltamethrin exposure on the Broiler’s liver histopathological feature, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activity. Fourty DOC Broilers strain New Loghman are divided into four group of ten and they were adapted for 5 days prior to the treatment. Group I (KI) is a control group, group II were given 20 mg/L deltamethrin, group III were given 10 mg/L deltamethrin and exposure deltamethrin concentration 10 mg/L and group IV were given 5 mg/L delthametrin. Deltamethrin was mixed with drinking water and then was given to the treatment group for 30 days. Blood samples were taken on day 0, day 15 and day 30 of treatment to determine of ALT and AST enzyme activity. On day 35, all animal were sacrificed, liver were taken out and fixed in 10% of buffer formalin for microscopic examination. Results of the AST enzyme activity shows that exposure to 20 mg/L and 10 mg/L deltamethrin for 30 days resulted in the histopathological changes of the liver, such as, fatty degeneration, necrosis on liver cells, inflamation of the liver, and necrosis on liver cells without the infiltration of inflamation cells. It is conclude that exposure to 20 mg/L deltamethrin for 30 days resulted in an increase in AST enzyme activity which is supported liver histopathological changes: fatty degeneration, necrosis, inflamation, and necrosis on liver cells without the infiltration of inflamation cells.

References

Abbassy MA dan Mossa AH. 2012. Haematobiochemical Effects of Formulated and Techical Cypermethrin and Deltamethrin Insectisides in Male Rats. Journal of Pharmacology and Toxicology.
Amacher DE. 1998. Serum Transaminase Elevations as Indicator of Hepatic Injury Following the Administration Oral. Journal Toxicology and Pharmacology. 119- 130.
Bag A dan Bag N. 2004. Target Sequence Polymorphism of Human Manganese Superoxide Dismute Gene and its association with Cancer Risk; a Review. Centre Cancer Epidemiology Biomarker prevention 12: 3298-305
Chargui I, Grissa I, Bensassi F, Hrira M.Y, Haouem S, Bencheikh H. 2012. Oxidative Stress, Biochemical and Histopathological Alterations in the Liver and Kidney of Female Rats Exposed to Low Doses of Deltamethrin: a Molecular Assessment. Biomedical and Enviroment Journal 6: 672-683
Coles EH. 1974. Veterinary Clinical Pathology. Ed Ke-2. Usuonders Company. Pp 219
Dash P. 2008. Apoptosis.http://www.sgul.ac.uk/. Diakses 10 Juni 2012.
Duncan’s dan Prasse’s. 2003. Veterinary Laboratory Medicine. Ed. Ke-4. Blackweel Publishing Company. Pp 195
Ellis R dan Rochville. 2003. Residue Of Some Veterinary Drugs In Animal And Foods Carbadox, Deltamethrin Dicyclanil, Flumequine, Imidocarb dipropoinatem Neomycin. Geneva: FAO Expert Committee.
Harju T, Kaarteenaho-Wiik R, Sirvio R, Crapo JD, Oury TD, Soini Y, Kinnula VL. 2004. Manganese superoxide Dismutase in Increased in The Airways of Smoker’s Lung’s. Europe Respiratory Journal 24: 765-771
Herlong HF. 1994. Approach To The Patient With Abnormal Liver enzymes. Hospital Practice 29: 32-38
Ince S, Kucukkurt I, Aytekin I, Barak E. 2010. Short-Term Effect of Deltamethrin Treatment on Oxidative Stress Biomarkers in Anatolian Water Buffaloes. Asian Journal of Animal and veterinary Advances 4: 266-270.
Jayasree UA, Gopala R, Reddy Y, Anjaneyulu B, Kalakumar. 2003. Evaluation Of Vitamin E Against Deltamethrin Toxicity In Brolier Chicks. Journal Pharmacology Science 4: 447-452
Lu FC. 1995. Toksikologi Dasar; Asas, Organ sasaran, dan Penilaian Resiko.Ed Ke-2. Jakarta: Penerbit Universsitas Indonesia Press.
Lumonga F. 2008. Apoptosis. Departemen Patologi Anatomo. Fakultas Kedokteran. Universitas Sumatera Utara. Medan. 1: 14
Marks DB dan Smith AD. 2000. Biokimia Kedokteran Dasar: Suatu Pendekatan Klinis, Penerjemah Suyono J, Sadikin V, Mandera LI. Jakarta: EGC.
McGregor DB. 2000. Pesticides Residue in Food 2000: Deltamethrin. International Agency For Research on Cancer. France
Patro N, Mishra SK, Chattopadhyay M, Patro IK. 1996.neurotoxicological Effects Of Deltamethrin on The Psotnatal developmental Of Cerebrum Of Rat. Journal Bioscice. 22: 177-130
Pryanto. 2009. Toksikologi Mekanisme, terapi Antidotum, Dan Penilaian Resiko.Ed Ke-1. Jawa Barat: Penerbit Lembaga Studi dan Konsultasi Farmakologi.
Staicu AC et al. 2007. Histological Changes In Deltamethrin-Induced Intoxication in Carrasius auratus gibelio (Pisces-cyprinidae). Biotechology in Animal Husbandary 23: 619 -626.
Stricker BHC. 1986. Hepatic Injury By Drugs And envinromental Agents. The Liver Annul 5: 419-482
Wang K dan Lin B. 2013. Pathofisiological Significance of Hepatic Apoptosis. Hindawi Publishing Corporation. 1:14
Zimmerman dan Maddrey. 1993. Toxic and Drug Induced Hepatitis in Schiff. Disease of the Liver.Ed. Key-7. Phyladelphia: Lippincot Company
Published
2019-06-01
How to Cite
Ndaong, N., Wijayanti, A., & Widyarini, S. (2019). Efek Pemaparan Deltamethrin pada Broiler Terhadap Aktivitas Enzim Alanin Aminotransferase, Aspartat Aminotransferase dan Gambaran Histopatologi Hepar. JURNAL KAJIAN VETERINER, 2(1), 79-87. https://doi.org/10.35508/jkv.v2i1.990

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.