Pengaruh Ukuran Ovarium Dan Diameter Oosit Terhadap Kualitas Morfologi Oosit Sapi Bali-Timor Yang Dikoleksi Secara In Vitro

  • Hermilinda Parera Program Studi Kesehatan Hewan Jurusan Peternakan Politeknik Pertanian Negeri Kupang
Keywords: oocyte diameter, ovary size, East Bali cattle, in vitro

Abstract

One of the reproductive technology in cattle which growing rapidly is in vitro fertilization techniques for in vitro embryo production. Environmental factors that influence the in vitro embryo development is the diameter of oocytes and ovarian size. Currently, the amount of research on Bali-Timor cattle is still lacking, especially in reproduction tecnology. Kune and Solihat (2007) states that the Bali-Timor cattle have a high fertility rate up to 60-70%. Application of in vitro reproduction technology can improve the genetic quality of livestock by using gametes cells collected from animals. The purpose of this study was to determine the effect of the size of the ovaries and oocytes diameter to the quality of the oocyte morphology of Bali-Timor cattle that were collected by in vitro technique. Oocytes samples of Bali-Timor cattle derived from ovarian which obtained from the slaughter house. Furthermore, the ovaries were grouped based on the size of the ovary, i.e group I (≤1,5 cm); group II (1.6 to 2 cm) and group III (≥2,1cm). Oocytes produced from the ovarian then grouped based on the diameter of the oocytes, i.e group I (110-150μm); group II (151-200μm); group III (201-250μm); group IV (251-300μm); Group V (301-350μm) and group VI (≥ 351μm). The results showed that the quality of the oocyte morphology did not differend significantly (P> 0.05) on ovarian size. Oocyte quality category A (29.78%) and category B (42.55%) were obtained from group III ovarian higher than group II (25% category A, category B 36%) and group I (category A 19.23 %, category B 26.92%), while the diameter of the oocytes had no effect on the percentage of morphological quality of oocytes produced in vitro.

References

Adnyana, IB. Putra. 2006. Hubungan Jumlah Folikel Antral Dengan Respon Ovarium terhadap Stimulasi Ovulasi. Jurnal Penyakit Dalam. Vol 7:3
Arlotto, T., J.L. Schwarctz, and N.L. First. 1996. Aspect follicle and oocyte stage that affect in vitro maturation and development of bovine oocytes. Theriogenology. 45:943–956.
Alvarez , A.M., Garcia. G. RM., Rebollar, P.G., Revuelta. L., Millan. P and Lorenzo PL. 2009. Influence of metabolic status on oocyte quality and follicular characteristics at different postpartum periods in primiparous rabbit does. Theriogenology, 72:612-623.
Baldassare H, Karatzas CN. 2004. Advanced Assisted Reproduction Technologies(Art) In Goats. Journal Animal Reproduction Science,82-83: 255-266.
Bilodeau-Goeseels, S. and P. Panich 2002. Effects of oocyte quality on development and transcriptional activity in early bovine embryos. Journal Animal Reproduction Science,71 (3-4): 143-155.
Darmadja, D. 1980. Setengah abad peternakan sapi tradisional dalam ekosistem pertanian di Bali. Thesis. Universitas Padjadjaran: Bandung.
De Wit, A.A., Y.A. Wurth, T.A. Kruip. 2000. Effect of ovarian phase and folliclequality on morphology and developmental capacity of the bovine cumulus- oocyte complex Journal Animal Reproduction Science, 78(5): 1277-1283.
Dominko, T. and N. Fisrt. 1992. Kinetics of bovine oocyte maturation and is affected by gonadotropins. Abstrac Theriogenology 37:203.
Gordon, I. 2003, Laboratory Production Of Cattle Embryos. Edisi ke-2. Dublin: CAB International
Hafez, E.S.E., dan B. Hafez. 2000. Folliculogenesis, egg maturation and ovulation. In: Hafez B and Hafez ESE. Reproduction in Farm Animals. 7th Ed. Philadelphia: Lea and Febiger. pp 68-81.
Hyttel, P., Fair .T., Callesen. H dan Greve T. 1997. Oocyte Growth, Capacitation dan Final Maturation in Cattle. Theriogenology, 47: 22-32.
Khurana, N.K. and H. Niemann. 2000. Effects of oocyte quality, oxygen tension,embryo density, cumulus cells and energy substrates on cleavage andmorula/blastocyst formation of bovine embryos.Theriogenology 54 (5): 741-756.
Krisher, R. L. 2004. The Effect of Oocyte Quality on Development. Journal Animal Science 82: E14-E23.
Lonergan, P., C. Carolan, A. Van Langendonckt, I. Donnay, H. Khatir and Mermillod. 1996. Role of Epidermal Growth Factor in Bovine Oocyte Maturation and Preimplantation Embryo Development In Vitro. Journal Biology of Reproduction. 54: 1420-1429.
Kune, P dan Solihat. N, 2007, Tampilan Berahi dan Tingkat Kesuburan Sapi Bali Timor yang Diinseminasi, Jurnal Ilmu Ternak 7:1-5
Lonergan, P., H. Sharif, and I. Gordon. 1992. Effect of time to transfer to granulosa cells monolayer on bovine oocyte developmental following IVM/IVF/IVC. Proceeding of the 8th Conference of the European EmbryoTransfer Association. 178.
Lonergan, P., P. Monaghan, D. Rizos, M.P. Boland, and I. Gordon. 1994. Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization, and culture in vitro. Mol. Reprod. and Developm. 37:48-53
Martino, A., T. Mogas, M.J. Palomo, and M.T. Paramio. 1994. Meiotic competence of prepubertal goat oocytes. Theriogenology. 41:968-980.
Toelihere, 1979. Fisiologi Reproduksi pada Ternak. Angkasa, Bandung.
Vivanco-Mackie HW. 2001. Embryo Transfer in Ovine and Caprine. In: Palma G. (ED), Biotechnology of Reproduction. Buenos Aires. Ediciones Inta.
Published
2019-06-01
How to Cite
Parera, H. (2019). Pengaruh Ukuran Ovarium Dan Diameter Oosit Terhadap Kualitas Morfologi Oosit Sapi Bali-Timor Yang Dikoleksi Secara In Vitro. JURNAL KAJIAN VETERINER, 2(2), 143-150. https://doi.org/10.35508/jkv.v2i2.998

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.