PENGARUH MUTASI GEN RYR-1 TERHADAP KUALITAS DAGING BABI LANDRACE
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Geertruida Margareth Sipahelut(1*)
universitas Nusa Cendana
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Muladno Muladno(2)
institut pertanian bogor
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Rudy Priyanto(3)
institut pertanian bogor
(*) Corresponding Author
Abstract
related to PSE pork. Forty animals were selected to produce 20 samples of each normal and PSE meat. DNA analysis was conducted for PSE pork, and meat quality attributes was measured for each types of meat, were drip loss, cooking loss, and tenderness (Warner-Bratzler Shear Force). Quantitatives data were analyzed using analysis of variance procedure. DNA analysis indicated there was any mutation at Ryr-1 gene for animals those produced PSE pork. There were highly significant differences between normal and PSE pork for three attributes of meat quality. Drip loss 1.65% ±0.16 vs 2.52%±0.17, cooking loss 28.79% ±2.64 vs 35.09 ± 2.38, and Warner-Bratzler Shear Force 5.57 kg/cm2±0.70 vs 7.18 kg/cm2± 0.70. The conclusion is that mutation at Ryr-1 gene caused animals produce lower quality of PSE pork.
ABSTRAK
Penelitian ini bertujuan untuk mengidentifikasi adanya mutasi dalam gen Ryr-1 dan kaitannya dengan kejadian PSE (pale, soft and exudatie) pada ternak babi yang diternakan secara komersial di Indonesia terhadap kualitas daging babi. Ternak yang digunakan dalam penelitian ini adalah 40 ekor babi keturunan Landrace jantan dan betina masing-masing 20 ekor menghasilkan daging normal dan 20 ekor menghasilkan daging PSE. Variabel kualitas daging yang diukur adalah susut drip, susut masak dan keempukkan serta pengujian DNA. Data kuantitatif diolah dengan menggunakan prosedur Sidik Ragam. Hasil penelitian menunjukkan susut drip daging babi normal sebesar (1.65±0.16) dan PSE (2.52±0.17), susut masak daging normal (28.79±2.64) dan PSE (35.09±2.38) serta keempukkan daging normal (5.57±0.70) dan PSE (7.18±0.70). Ketiga variabel menunjukkan perbedaan yang signifikan dan hasil analisis DNA menunjukkan terjadi mutasi pada gen Ryr-1. Kesimpulan daging babi PSE mempunyai kualitas yang lebih rendah daripada daging babi normal. Sebagaimana diharapkan, babi yang menghasilkan daging PSE mengalami mutasi pada gen Ryr-1 berdasarkan analisis PCR-RFLP.
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References
Boles, J.A.., F.C Parrish, Jr., T.W. Huaitt, and R.M. Robson. 1992. Effect of the porcine stress syndrome on the solubility and degradation of myofibrillar/cytoskeletal proteins. J. Anim. Sci. 70:545-464
Byrem, T.M., A.M. Booren, G.M. Hill, Fun Sun Chu and G.M. Strasburg. 1999. The effect of cyclopiazonic acid on the development of pale, soft, and exudative pork from pigs of devined Malignant Hyperthermia genotype. J. Anim. Sci.77:166-172.
Cheah, K.S., A.M. Cheah, A.R. Crosland, J.C. Casey and A.J. Webb. 1984. Relationship between Ca2+ release, sarcoplasmic Ca2+, glycolysis and meat quality in halothane-sensitive pigs. Meat Sci. 10:117.
de Smet, S., H. Pauwels, W. Eeckhout, D. Demeyer, I.Vervaeke, S. de Bie, G. Van De Voorde and M. Casteels. 1992. Relatioships between halothane sensitivity, carcass quality and meat quality in Belgian slaugther pigs. In Pork Quality: Genetic and Metabolic Fa. ctors. OECD Workshop. Ed. by E. Puolanne and D.I. Demeyer. Finland, Helsinki. Pp 273-286.
de Smet, S., H. Pauwels, I. Vervaeke, D. Demeyer, S. de Bie, W. Eeckhout and M. Casteels. 1995. Meat and carcass quality of heavy muscled Belgian slaugther pigs as influenced by halothane sensitivity and breed. Anim. Sci. 61:109-114.
Estrade, M., E. Rock and X. Vignon. 1991. Ultrastructural post mortem changes n myofibrillar structure on normal and halothane-sensitive pigs. Proc. of the 37th Internasional Congress of Meat Science and Technology. Vol. 1. pp 352-355. Kulmbach, German.
Fuji, J., K. Otsu, F. Zorzato, S. de Leon, V.K. Khanna, J.E. Weiler, P.J. O’Brien and D.H. MacLennan. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthemia. Science, 253:448-451.
Judge, M.D., E.D. Aberle, J.C. Forrest, H.B. Hendrick and R.A. Merkel. 1989. Princeples of Meat Science. Kendal/Hunt Publishing Company, Iowa.
Leach, L.M., M. Ellis, D.S. Sutton, F.K. McKeith, and E.R. Wilson. 1996. The growth performance, carcass characteristics, and meat quality of halothane carrier and negative pigs. J. Anim. Sci. 74:934-943.
Kang, C.G., M. Mugumura, T. Fukazawa, and T. Ito. 1978. Difference in Z-line removal between normal and PSE porcine myofibrils. J. Food. Sci. 43:508
Lundstrom, K.A. Karrison, I. Hansson., M. Johansson, L. Andersson and K. Andersson. 1995. Production, carcass and meat quality traits of F2-crosses between European Wild Pigs and domestic pigs including halothane gene carries. Anim. Sci. 61:325-331.
Marby, J.W., L.L. Christian and D.L. Kuhlers. 1981. Inheritance of porcine stress syndrome. The of Heredity 72:429-430
Mc Farlane, B.J and J.A. Unruh. 1996. Effect of blast chilling and postmortem calsium chloride injectio on tenderness of pork longissimus muscle. J. Anim. Sci. 74:1842-1845.
Monin, G., C. Larzul, P. Le Roy, J. Culioli, J Mourut, S. Rousset-Akrim, A. Talmant, C. Touraille and P. Sellier. 1999. Effects of halothane genotype and slaugther weigth on texture of pork. J. Anim. Sci. 77: 408-415.
Muladno, R. Priyanto and S. Sulandari. 1999. Identification of Porcine Stress Syndrome in commercial pigs associated quality. One Day Seminar on Science and Technology. Indonesia Toray Science Foundation, Jakarta.
Otsu, K., M.S. Philips, V.K. Khanna , S. de Leon, and D.H. MacLennan. 1992. Refinemennt of diagnostic assay for probable casual mutation for porcine ryanodine and human malignant hyperthemia. Genomics, 13:835-837.
Preston, T.R. and M.B. Willis. 1979. Intensive Beef Production. Pergamon Press, Oxford.
Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning, A Laboratory Manual. Second Edition. Cold Spring Harbour Lab. Press. New York.
SAS. 1987. SAS/STAT Guide for Personal Computer version 6 Edition, Statiscal Analysis System Institute, Inc., Cary, NC, USA.
Soeparno. 2009. Ilmu dan Teknologi Daging. Gajah Mada University Press, Yogyakarta.
Swatland, H.J. 1992. Paleness, softness and exudation in pork – Review. Pork Quality: Genetic and Metabolic Factors. OECD Workshop. Finland, Helsinki. Ed by E. Puolanne and D.I. Demeyer. pp 273-286.
Tegelstrom, H. 1992. Mithocondrial DNA in natural population: An improved routine for the screening of genetic variation based on sentsitive silver staining. Electrophoresis 7:226-229.
van Lack, H.L., J.M. and F.J.M. Smulders. 1999b. Chilling rate and pork quality-an orientation. Proc. Of the 37th International Congress of Meat Science and Technology. Vol. 1. pp 154-156. Kulmbach, German.
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