Viabilitas Spermatozoa Semen Beku Babi Duroc Dalam Extender Beltsville Thawing Solution Menggunakan Krioprotektan Gliserol Dan Dimetillacetamida
-
Nancy D. F. K. Foeh(1*)
Laboratorium Klinik, Reproduksi, Patologi dan Nutrisi Fakultas Kedokteran Hewan Universitas Nusa Cendana
-
Raden Iis Arifiantini(2)
Departemen Klinik, Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
-
Tuty Laswardy Yusuf(3)
Departemen Klinik, Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(*) Corresponding Author
Abstract
This study investigated the effect of glycerol and dimethylacetamide (DMA) in beltsville thawing solution (BTS) extender. Semen samples were collected from three boars of the Duroc breed (n=5) and were evaluated microscopically and macroscopically. Semen that have been evaluated and meets the following criteria: has motility characteristics >70%, concentration >300 million/ml and abnormality 20%; was accepted as appropriate samples. Semen samples were divided into four tubes and were diluted with 5 ml of BTS. The diluted semen was left for two hours in the temperature range from 20 to 22°C before was centrifuged for 15 minutes (500G). The pellet of centrifuged semen was taken as much as 1 ml with its supernatant. The BTS glycerol (BTSG), BTS-DMA (BTSD), BTS-Glycerol-
DMA (BTSG-D) were added into the pellet. The diluted semen was packed into straw of 0.5 ml and was equilibrated for 2 hours in 4 °C. The equilibrated semen was frost with the steam of nitrogen and stored in liquid nitrogen. The quality of frozen semen was assessed at least 24 hour after freezing process. The results indicate that the sperm motility after thawing in BTS extender BTSD is 52.14±0.4%, ,higher than the other two extenders (P<0.05). Thus, it is conclude that the DMA concentration in BTS extender maintains the quality of frozen semen from the Duroc breed better than other extenders.
Downloads
References
Arifiantini RI. 2012. Teknik Koleksi dan Evaluasi Semen pada Hewan. IPB press: Bogor. Pp 69-71.
Gadea J. 2003. Semen extenders used in the artificial insemination of Swine. J of Agri Res.1(2): 17-27
Garner DL, Hafez ESE. 2000. Spermatozoa and seminal plasma. In: Hafez B, Hafez ESE, editor. Reproduction in Farm Animals. 7thEd. Philadelphia (US): Lippincott
Williams and Wilkins. Pp 96-109.
Graham LH, Bando J, Gray C, Buhr MM. 2004. Liquid storage of asian (Elephas Maximus) sperm at 4 oC. J Anim Reprod Sci. 80: 329-340.
Johnson LA, Weitze KF, Fiser P, Maxwell WMC. 2000. Storage of boar semen. J Anim Reprod Sci. 62: 143-172.
Leboeuf B, Restall B, Salamon S. 2000. Production and storage of goat semen for artificial insemination. J Anim Reprod Sci. 62: 113-141
Medeiros ASL, Gomes GM, Rarmo MP, Papa FO, Alverangga MA. 2002. Cryopreservation of stallion sperm using different amides. Theriogenology. 58: 273-276.
Purdy PH. 2006. A review on goat sperm cryopreservation. J Small Ruminant Res. 63: 215-225
Paulenz H, Kommisrud E, Hofmof PO. 2000. Effect of long-term storage at different temperatures on the quality of liquid boar semen. J Anim Reprod Dom. 35: 83-85.
Steel RGD, Torrie JH. 1995. Prinsip Dan Prosedur Statistika: Suatu Pendektan Biometrik. Sumantri B, penerjemah. Jakarta: Gramedia Pustaka Utama.
Yi JK, Ko HJ, Lee HS, Yang BC, Park CS. 2008. In vitro fertilizationand development of pig oocytes inseminated with boar sperm washing media after thawing of the
frozen straw. J Asian-Aus Anim Sci. 17(2): 164-167
.png)
