Uji Efektivitas Ekstrak Daun Rumput Paitan (Paspalum conjugatum) terhadap Kesembuhan Luka Insisi pada Tikus Putih (Rattus norvegicus)
Efficacy Test of Paitan Grass (Paspalum conjugatum) Leaves Extract on Healing of Incision Wounds in White Rats (Rattus norvegicus
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Andreas E. Porat(1*)
Program Studi Kedokteran Hewan, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang
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Meity M. Laut(2)
Laboratorium Anatomi, Fisiologi, Farmakologi, dan Biokimia, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang
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Nemay A. Ndaong(3)
Laboratorium Anatomi, Fisiologi, Farmakologi, dan Biokimia, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang
(*) Corresponding Author
Keywords:
extract, incision wound, paitan grass leaves, paspalum conjugatum, white rat
Abstract
Indonesia has the largest biodiversity in the world (Megabiodiversity), including Paitan Grass (Paspalum conjugatum) or what will hereinafter be called P. conjugatum, which is a forage plant for livestock (HPT). In several regions such as the Philippines, Kalimantan, Papua New Guinea and Manggarai, this grass is commonly used as a wound medicine. This study aims to determine the effectiveness of Paitan Grass leaf extract on incisional wounds in white rats. The method used was laboratory experimental, using Paitan Grass (P. conjugatum) which was extracted using a maceration technique with 70% ethanol solvent for 3 days, and applied to white rats (Rattus norvegicus) which had been incised in vivo. This study had 3 groups, namely positive control (povidone iodine 10%), negative control (NaCl 0.9%), and Paitan Grass extract. The parameters for observing incisional wound healing in this research were the length of time, speed of hemostasis, wound color, exudate and crusting, and the length of time for wound healing which was measured for 14 days. Measured using a caliper. The results obtained were analyzed using One Way ANNOVA Tukey HSD advanced test. The results showed that Paitan Grass (P. conjugatum) extract was effective in speed of hemostasis (P>0.05) with positive control, color change in 48 hours, exudate visible in 24 hours, crusting and speed of wound healing (P<0.05 ) with both control groups, but there was no significance (P>0.05) between the positive control and negative control.
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Desmouliere, A., Darby, I. A., Laverdet, B., & Bonté, F. (2014). Fibroblasts and myofibroblasts in wound healing. Clinical, Cosmetic and Investigational Dermatology, 301. https://doi.org/10.2147/CCID.S50046
Endarini, L. H. (2019). Analisis Rendemen Dan Penetapan Kandungan Ekstrak Etanol 96% Daun Teh Hijau (Camellia sinensis L.,) Dengan Metode Kromatografi Lapis Tipis . SEMNASKes.
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Garduque, D. A. P., Mateo, K. R. G., Oyinloye, S. M. A., & Lucero, J. A. kristine L. (2019). Antimicrobial Efficacy of Carabao Grass (Paspalum conjugatum) leaves on Staphylococcus aureus. Abstract Proceedings International Scholars Conference, 7(1), 384–397. https://doi.org/10.35974/isc.v7i1.1035
Hasim, H., Arifin, Y. Y., Andrianto, D., & Faridah, D. N. (2019). Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi. Jurnal Aplikasi Teknologi Pangan, 8(3), 86. https://doi.org/10.17728/jatp.4201
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Kemenkes RI. (2017). Farmakope Herbal Indonesia (2nd ed.). Kementerian Kesehatan RI.
Larouche, J., Sheoran, S., Maruyama, K., & Martino, M. M. (2018). Immune Regulation of Skin Wound Healing: Mechanisms and Novel Therapeutic Targets. Advances in Wound Care, 7(7), 209–231. https://doi.org/10.1089/wound.2017.0761
Li, K., Diao, Y., Zhang, H., Wang, S., Zhang, Z., Yu, B., Huang, S., & Yang, H. (2011). Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats. BMC Complementary and Alternative Medicine, 11(1), 86. https://doi.org/10.1186/1472-6882-11-86
Marcińczyk, N., Gromotowicz-Popławska, A., Tomczyk, M., & Chabielska, E. (2022). Tannins as Hemostasis Modulators. Frontiers in Pharmacology, 12. https://doi.org/10.3389/fphar.2021.806891
McGuire, J., & Nasser, J. (2021). Redefining Slough: A New Classification System to Improve Wound Bed Assessment and Management. Wounds : A Compendium of Clinical Research and Practice, 33(8), E61–E66. https://doi.org/10.25270/wnds/2021.e6166
Mohammed, M. S., Osman, W. J. A., Garelnabi, E. A. E., Osman, Z., Osman, B., Khalid, H. S., & Mohamed, M. A. (2014). Secondary metabolites as anti-inflammatory agents. The Journal of Phytopharmacology, 3(4), 275–285. https://doi.org/10.31254/phyto.2014.3409
Nurdiantini, I., Prastiwi, swito, & Nurmaningsari, T. (2017). Perbedaan Efek Penggunaan Povidone Iodine 10% Dengan Minyak Zaitun Terhadap Penyembuhan Luka Robek (Lacerated Wound). Nursing News, 2(1).
Padalhin, A., Ryu, H. S., Yoo, S. H., Abueva, C., Seo, H. H., Park, S. Y., Chung, P.-S., & Woo, S. H. (2024). Antiseptic, Hemostatic, and Wound Activity of Poly(vinylpyrrolidone)-Iodine Gel with Trimethyl Chitosan. International Journal of Molecular Sciences, 25(4), 2106.
https://doi.org/10.3390/ijms25042106
Rahmawati, R., Ain, H., & Marsaid. (2019). Pengaruh Pemberian Topikal Gel Lidah Buaya (Aloe Vera) Terhadap Gambaran Makroskopis Penyembuhan Luka Sayat Pada Tikus Putih (Rattus Norvegicus) Galur Wistar. Poltekes Kemenkes Malang.
Saleh, N. S. (2016). Formulasi dan Uji Efektivitas Gel Ekstrak Daun Jambu Biji (Psidium guajava L.) Terhadap Penyembuhan Luka Bakar Pada Kelinci Jantan (Oryctolagus cuniculus). universitas negeri gorontalo.
Sandoval, J. R. (2018). Paspalum conjugatum (buffalo grass). CABI Compendium.
Slamet, A. H. H., & Soedibyo, D. W. (2019). Sorting Manalagi Apple (Malus sylvetris Mill) using Image Processing Application. Food ScienTech Journal, 1(2), 105. https://doi.org/10.33512/fsj.v1i2.7093
Sucita, R. E., Hamid, I. S., Fikri, F., & Purnama, M. T. E. (2019). Ekstrak Etanol Kayu Secang (Caesalpinia sappan L.) Secara Topikal Efektif pada Kepadatan Kolagen Masa Penyembuhan Luka Insisi Tikus Putih. Jurnal Medik Veteriner, 2(2), 119–126.
Suharto, I. P. S., & Etika, A. N. (2019). Ekstrak Jahe (Zingiber Officinale Roscoe) Berpengaruh Terhadap Kepadatan Serabut Kolagen Luka Insisi. Care : Jurnal Ilmiah Ilmu Kesehatan, 7.
Suryadi, I. A., Asmarajaya, A., & Maliawan, S. (2020). Proses Penyembuhan Dan Penanganan Luka.
Umi Lathifah. (2020). Identifikasi Senyawa Metabolit Sekunder Dan Aktivitas Antioksidan Daun Tebu (Saccharum offinarum L.) [Biologi]. Universitas Negeri Semarang.
Wang, C., Zhou, H., Niu, H., Ma, X., Yuan, Y., Hong, H., & Liu, C. (2018). Tannic acid-loaded mesoporous silica for rapid hemostasis and antibacterial activity. Biomaterials Science, 6(12), 3318–3331. https://doi.org/10.1039/C8BM00837J
Yunita, E., & Khodijah, Z. (2020). Pengaruh Konsentrasi Pelarut Etanol saat Maserasi terhadap Kadar Kuersetin Ekstrak Daun Asam Jawa (Tamarindus indica L.) secara Spektrofotometri UV-Vis. PHARMACY: Jurnal Farmasi Indonesia , 17, 273–280.
Zakariya, M., Sudiana, I. K., & Wahyuni, E. D. (2009). Efektivitas Perawatan Luka Insisi Dengan Madu Dan Povidon Iodin 10% . Jurnal Ners , 4(1).
Published
2024-12-29
How to Cite
Porat, A., Laut, M., & Ndaong, N. (2024). Uji Efektivitas Ekstrak Daun Rumput Paitan (Paspalum conjugatum) terhadap Kesembuhan Luka Insisi pada Tikus Putih (Rattus norvegicus). JURNAL KAJIAN VETERINER, 12(2), 207-220. https://doi.org/10.35508/jkv.v12i2.17032
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