Molecular Identification of Babesia bigemina from Cattle and Buffaloes in Bogor District
Identifikasi Molekular Babesia bigemina dari Sapi dan Kerbau di Kabupaten Bogor
-
Arifin Budiman Nugraha(1*)
Sekolah Kedokteran Hewan dan Biomedis, Institut Pertanian Bogor, Bogor
-
Umi Cahyaningsih(2)
Sekolah Kedokteran Hewan dan Biomedis, Institut Pertanian Bogor, Bogor
-
Jyn Soong Qing Wen(3)
Sekolah Kedokteran Hewan dan Biomedis, Institut Pertanian Bogor, Bogor
-
Hadri Latif(4)
Sekolah Kedokteran Hewan dan Biomedis, Institut Pertanian Bogor, Bogor
(*) Corresponding Author
Keywords:
Babesia bigemina, babesiosis, bogor, kerbau, pcr, sapi
Abstract
Peternakan merupakan industri penting yang memberikan kontribusi signifikan terhadap sektor pertanian di Indonesia. Babesiosis adalah penyakit yang ditularkan melalui vektor caplak dan disebabkan oleh parasit darah genus Babesia. Penyakit ini telah menyebabkan kerugian ekonomi yang sangat signifikan. Deteksi molekular babesiosis memiliki sensitivitas dan spesifisitas yang tinggi. Namun, saat ini, deteksi babesiosis menggunakan Polymerase Chain Reaction (PCR) di Indonesia belum banyak digunaknan, khususnya di Kabupaten Bogor. Tujuan penelitian ini adalah untuk mengidentifikasi Babesia bigemina pada sapi dan kerbau di Kabupaten Bogor dengan teknik molekular. Sebanyak 27 sampel darah dikoleksi yang terdiri atas 22 sampel sapi dan 5 sampel darah kerbau. Sampel diwarnai dengan Giemsa 10%, selain itu sampel darah diekstraksi dan dilanjutkan dengan nested PCR. Hasil dari pemeriksaan apusan darah menunjukkan 81,48% positif terhadap Babesia spp, sedangkan hasil nested PCR 11,11% menunjukkan positif terhadap B. bigemina. Berdasarkan jenis hewan sapi dan kerbau positif terhadap B. bigemina, masing-masing sebesar 9,09% dan 20%. Hasil penelitian ini memberikan informasi dasar mengenai tingkat infeksi Babesia bigemina di kabupaten Bogor berdasarkan metode molekuler. Oleh karena itu, program pencegahan dan pengendalian terhadap infeksi parasit darah pada peternakan sapi dan kerbau sangat perlu dilakukan.
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References
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Adewoyin AS, Nwogoh B. 2014. Peripheral blood film - a review. Ann Ib Postgrad Med. 12(2):71–9.
AL-Hosary AAT. 2017. Comparison between conventional and molecular methods for diagnosis of bovine babesiosis (Babesia bovis infection) in tick infested cattle in upper Egypt. J Parasit Dis. 41(1):243–246. doi:10.1007/s12639- 016-0785-2.
Alvarez JA, Rojas C, Figueroa J V. 2019. Diagnostic Tools for the Identification of Babesia sp. in Persistently Infected Cattle. Pathogens. 8(3):143. doi:10.3390/pathogens8030143
Avenant A, Park JY, Vorster I, Mitchell EP, Arenas-Gamboa AM. 2021. Porcine Babesiosis Caused by Babesia sp. Suis in a Pot-Bellied Pig in South Africa. Front Vet Sci. 7. doi:10.3389/fvets.2020.620462.
Bock R, Jackson L, De Vos A, Jorgenson W. 2004. Babesiosis of cattle. Parasitology. 129(S1):S247–S269. doi:10.1017/S0031182004005190.
Dyahningrum DM, Mufasirin, Harijani N, Hastutiek P, Koesdarto S. 2019. Identification of Blood Parasite on Sacrifical Cattle Slaughtered during Idul Adha 1438 H in Surabaya City and Sidoarjo Regency. J Parasit Sci. 3(2).
Ganzinelli S, Benitez D, Gantuya S, Guswanto A, Florin‐Christensen M, Schnittger L, Igarashi I. 2020. Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina. Transbound Emerg Dis. 67(S2):159–164. doi:10.1111/tbed.13435.
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Giglioti R, Okino CH, Azevedo BT, Wedy BCR, Gutmanis G, Veríssimo CJ, Katiki LM, Filho AEV, de Oliveira HN, Oliveira MC de S. 2021. Semiquantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis. Ticks Tick Borne Dis. 12(5):101753. doi:10.1016/j.ttbdis.2021.101753.
Githaka NW, Bishop RP, Šlapeta J, Emery D, Nguu EK, Kanduma EG. 2022. Molecular survey of Babesia parasites in Kenya: first detailed report on occurrence of Babesia bovis in cattle. Parasit Vectors. 15(1):161. doi:10.1186/s13071-022-05279-7
Goff WL, Molloy JB, Johnson WC, Suarez CE, Pino I, Rhalem A, Sahibi H, Ceci L, Carelli G, Adams DS, et al. 2006. Validation of a Competitive EnzymeLinked Immunosorbent Assay for Detection of Antibodies against Babesia 17 bovis. Clin Vaccine Immunol. 13(11):1212–1216. doi:10.1128/CVI.00196- 06.
Gray J, Zintl A, Hildebrandt A, Hunfeld K-P, Weiss L. 2010. Zoonotic babesiosis: Overview of the disease and novel aspects of pathogen identity. Ticks Tick Borne Dis. 1(1):3–10. doi:10.1016/j.ttbdis.2009.11.003.
J. Mosqueda, A. Olvera-Ramirez, G. Aguilar-Tipacamu, G. J. Canto. 2012. Current Advances in Detection and Treatment of Babesiosis. Curr Med Chem. 19(10):1504–1518. doi:10.2174/092986712799828355.
Jaimes-Dueñez J, Triana-Chávez O, Holguín-Rocha A, Tobon-Castaño
A, MejíaJaramillo AM. 2018. Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia. Parasit Vectors. 11(1):510. doi:10.1186/s13071-018-3091-2.
Jalovecka M, Hajdusek O, Sojka D, Kopacek P, Malandrin L. 2018. The Complexity of Piroplasms Life Cycles. Front Cell Infect Microbiol. 23(8): 248. doi:10.3389/fcimb.2018.00248
Laha R, Das M, Sen A. 2015. Morphology, epidemiology, and phylogeny of Babesia: An overview. Trop Parasitol. 5(2):94. doi:10.4103/2229- 5070.162490.
Mahmoud MS, Kandil OM, Nasr SM, Hendawy SHM, Habeeb SM, Mabrouk DM, Silva MG, Suarez CE. 2015. Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt. Parasit Vectors. 8(1):319. doi:10.1186/s13071-015-0928-9.
Meredith S, Oakley M, Kumar S. 2021. Technologies for Detection of Babesia microti: Advances and Challenges. Pathogens. 10(12):1563. doi:10.3390/pathogens10121563.
Nugraheni YR, Ariyadi B, Rochmadiyanto R, Kesumaningrum N, Imran K, Kartiko BP, Farhani NR, Nurani S, Sahara A, Awaludin A. 2023. Molecular detection of Babesia infection in cattle in Yogyakarta, Indonesia. Biodiversitas. 24(7). doi:10.13057/biodiv/d240759.
Putra GLA, Yonathan CJ, Niedhatrata NI, Rizka Firdaus MH, Yoewono JR. 2020. A review of the development of Polymerase Chain Reaction technique and its uses in Scientific field. Stannum : J Kim Terap Indones. 2(1):14–30. doi:10.33019/jstk.v2i1.1619
Romero-Salas D, Mira A, Mosqueda J, García-Vázquez Z, Hidalgo-Ruiz M, Vela NAO, de León AAP, Florin-Christensen M, Schnittger L. 2016. Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field. Vet Parasitol. 217:101–107. doi:10.1016/j.vetpar.2015.12.030.
Suarez CE, Alzan HF, Silva MG, Rathinasamy V, Poole WA, Cooke BM. 2019. Unravelling the cellular and molecular pathogenesis of bovine babesiosis: is the sky the limit? Int J Parasitol. 49(2):183–197. doi:10.1016/j.ijpara.2018.11.002.
Tona GO. 2023. A Global Overview of the Intensification of Beef and Dairy Cattle Production Systems. Di dalam: Intensive Animal Farming - A Cost-Effective Tactic. doi: 10.5772/intechopen.106062
[WOAH] World Organisation for Animal Health. 2020. Bovine Babesiosis. Paris: World Organisation for Animal Health.
Published
2024-12-17
How to Cite
Nugraha, A., Cahyaningsih, U., Qing Wen, J. S., & Latif, H. (2024). Molecular Identification of Babesia bigemina from Cattle and Buffaloes in Bogor District. JURNAL KAJIAN VETERINER, 12(2), 161-169. https://doi.org/10.35508/jkv.v12i2.17799
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Articles
Copyright (c) 2024 JURNAL KAJIAN VETERINER
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