Characterisation Of E. coli Virulence Factors Causing Diarrhea In Macaca Fascicularis At IPB University Captivity, Bogor, Jawa Barat, Indonesia
Karakteristik Faktor Virulensi E. coli Penyebab Diare Pada Penangkaran Macaca Fascicularis Di IPB University, Bogor, Jawa Barat, Indonesia
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Muhammad Farid Rizal(1)
Doctorate Student of Primatology, Primatology program, School of postgraduate, IPB University, Bogor
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I Wayan Teguh Wibawan(2*)
School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Primate Research Center IPB University, Bogor
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Joko Pamungkas(3)
School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor,
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Roostita L. Balia(4)
Veterinary Study Program, Faculty of Medicine, Universitas Padjajaran, Sumedang
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Huda Shalahudin Darusman(5)
School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor
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Maryati Surya(6)
Primate Research Center IPB University, Bogor
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Yusuf Farid Achmad(7)
Departement Research and Development, PT. Biomol Nusantara Mandiri, Kec. Panyileukan, Bandung
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Betty Sundari(8)
5Departement Research and Development, PT. Biomol Nusantara Mandiri, Kec. Panyileukan, Bandung
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Yovi Alwi Soedarso(9)
Departement Research and Development, PT. Biomol Nusantara Mandiri, Kec. Panyileukan, Bandung
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Yovi Alwi Soedarso(10)
Departement Research and Development, PT. Biomol Nusantara Mandiri, Kec. Panyileukan, Bandung
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Denniswara Sibit(11)
Departement Research and Development, PT. Biomol Nusantara Mandiri, Kec. Panyileukan, Bandung
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Fhady Risckhy Loe(12)
Departement Animal Diseases and Veterinary Public Health, Veterinary Study Program, Faculty of Medicine and Veterinary Medicine Universitas Nusa Cendana Kupang
https://orcid.org/0009-0000-1969-1399
(*) Corresponding Author
Abstract
Diare pada Macaca fascicularis sangat mengganggu proses penangkaran, diare mengurangi kualitas hidup kera, E. coli sering dilaporkan sebagai penyebab utama diare pada beberapa primata non-manusia. Tujuan dari penelitian ini adalah untuk mendeteksi faktor virulensi seperti hemolisin, pola hemaglutinin, dan hidrofobisitas permukaan sel. Penelitian ini menggunakan 30 isolat E. coli dari koleksi pusat penelitian primata IPB University yang diisolasi dari kera ekor panjang yang menderita diare. Faktor virulensi seperti hemolisin, hemaglutinasi, dan agregasi garam dideteksi sesuai protokol standar. Sebanyak 30 isolat M. fascicularis yang menderita diare diuji kemampuannya menyebabkan lisis pada eritrosit, di antara 24 (80%) sampel E. coli menunjukkan α -hemolisin pada agar darah dan 2 (6,67%) sampel menunjukkan γ-hemolisis. Sebanyak 21 (70%) sampel E. coli mampu menghemaglutinasi eritrosit sapi dan 27 (90%) sampel mampu menghemaglutinasi eritrosit kelinci. Sebanyak 30 sampel E. coli tidak mampu menghemaglutinasi eritrosit ayam, kucing, anjing, dan manusia. Sebanyak 2 (6,67%) sampel menunjukkan hasil positif pada uji agregasi garam di antara 30 sampel E. coli dari M. fascicularis dan sisanya 28 (93,33%) sampel tidak menunjukkan hasil positif pada uji agregasi garam. Kesimpulan penelitian adalah Sebanyak 30 isolat E. coli menunjukkan variasi faktor virulensi yang berperan dalam mekanisme diare pada Macaca fascicularis. Terdapat 24 isolat (80%) yang menunjukkan α-hemolisin, 2 isolat (6,67%) menunjukkan γ-hemolisin, dan 4 isolat lainnya (13,33%) tidak menunjukkan aktivitas hemolisis. Selain itu, 29 isolat (96,7%) menunjukkan hemaglutinasi terhadap eritrosit sapi dan/atau kelinci, sedangkan 2 isolat (6,7%) bersifat hidrofobik berdasarkan uji salt aggregation.
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