EVALUASI TITER ANTIBODI PASCA VAKSINASI Septicaemia epizootica PADA SAPI BALI DI KOTA KUPANG
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Mario H.Cantona(1*)
Universitas Nusa Cendana
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Maxs Urias Ebenheizer Sanam(2)
Universitas Nusa Cendana
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Tri Utami(3)
Universitas Nusa Cendana
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Tarsisius Considus Tophianong(4)
Universitas Nusa Cendana
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Antin Y.N Widi(5)
Universitas Nusa Cendana
(*) Corresponding Author
Keywords:
Antibody titer, Post vaccination, Septicaemia epizootica, indirect ELISA
Abstract
Controlling Septicemia epizooticae (SE) through vaccination program has been undertaken in Kupang City. However, numbers of fatal cases are still being reported. The purpose of this study is to measure the antibody titer of Bali cattle after SE vaccination, and to determine the effect of age and sex on antibody titers. The 50 serum samples of SE vaccinated Bali cattle were taken from Alak Sub-district (26 samples) and Maulafa Sub-district (24 samples). The selection of sub-districts in Kupang City was taken in a simple random manner. Those serum samples were examined using the indirect enzyme-linked immunosorbent assay (ELISA) method. Antibody titers against SE is declared to be protective when the antibody titer is above 88 ELISA Unit (EU). Results indicated that average value of cattle antibody titer after the SE vaccination was able to trigger a protective antibody response (> 70 EU), meanwhile ONE WAY ANOVA analysis results showed that there is no significant effect (P> 0.05) of cattle age towards antibody titers. In the same way, the paired t test results did not indicate a difference in the value of antibody titers against the sex of the Bali cattle.
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References
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Shivachandra S.B., Nagaleekar V.K., Kumar A.A. 2011, A Review of Haemorrhagic Septicemia in Cattle and Buffalo.An H Res 12(1): 67-82.
Soeripto. 2002, Pendekatan Konsep Kesehatan Hewan melalui Vaksinasi, Jurnal Penelitian dan Pengembangan Pertanian 21:48-55.
Sugiyono. 2012, Metode Penelitian Administrasi. Cetakan ke-20. Penerbit Alfabeta. Bandung.
Suwarno. 2003, Prinsip Dasar Optimalisasi dan Interpretasi Hasil Uji ELISA, Lab Virologi dan Imunologi FKH Unair. Surabaya.
Tizard I.R. 2004, Veterinary Immunology an Introduction. 7th Ed. USA: Saunders.
Ahmad M., Aziz M., Tanio T. M., Rehman R., Rehman A., Rehman K., Hameed A., Naveed T., Tanveer M., Ali M. 2018, Seroprevalence of Hemorrhagic septicemia in Buffalo and Cattle in Flood, Irrigated, and Sandy Area of Punjab, Pakistan. Pure and Applied Biology. 7(4):1234-1243.
Alwis De, M. C. L. 1999, Haemorrhagic Septicaemia, ACIAR Monograph, Australia.
Arocho, F.J., Maldonado, K.A., Bradley. 2009, Infection and Immnunity, Cell Microbiol, American Society of Microbiology.
Astuti Lilis Sri., Istiyaningsih., Khairul Daulay., Sarji., Deden Amijaya., Neneng Atikah., Meutia Hayati., Ernes Andesfha. 2014, Studi Mutu Vaksin Septicemia epizootica (SE) dan Durasi Imuniti Booster dan non Booster Vaksinasi pada Sapi di Empat Provinsi di Indonesia Tahun 2014. Unit Uji Bakteriologi, Balai Besar Pengujian dan Sertifikasi Obat Hewan, Gunungsindur–Bogor16340.
Bailie, L. 2001, The Development of New Vaccines against Bacillus anthracis. UK : Journal of Applied Microbiology.
Benkirane A., De Alwis M.C.L. 2002, Haemorrhagic Septicaemia, Its Significance, Prevention and Control in Asia. Vet.Med-Czech.47(8): 234-240.
Berek H.S. Debora., Nugroho S. Widagdo., Wahyuni A.E.T. Hastuti. 2015, Protektivitas Sapi di Kabupaten Kupang terhadap Penyakit Ngorok (Septicaemia Epizootica). Jurnal Veteriner.
Besung I.N.K., I Gusti Ketut Suarjana, Ketut Tono P.G., Ni Ketut Suwiti. 2017,Seroepidemiologi Septichaemia epizootica berdasarkan Jenis Kelamin pada Sapi Bali di Sumbawa. Bul Vet Udayana 9(1): 42-46
Besung I.N.K., Tono P.G.K., Rompis A.L.T., Suarjana I.G.K. 2016, Prevalensi Pasteurella multocida pada Sapi Bali di Bali. Bul. Vet Udayana 8(2): 145-150.
Dewi F.N.A. 2006, Deteksi Antibodi Human Immunodeficiency Viru Type-1 (HIV1) pada Macaca Nemestrina [skripsi].Bogor (ID): Institut Pertanian Bogor.
Farooq U., Hussain M., Irshad H., Badar N., Munir R., and Ali Q. 2007, Status Haemorrhagic Septicaemia based on Epidemiology In Pakistan. Pakistan Vet.J. 27(2): 67-72.
Field, Thomas G., Taylor, Robert E. 2012, Scientific Farm Animal Production. 10th ed. Pearson. United State of America.
Hajikolaei M., Ghorbanpour R.H.M., Sayfi Abadshapouri M.R., Rasooli A., Moazeni-Jula A.G.R., Ebrahimkhan D. 2008, Study on The Prevalence of Pasteurella multocida Carriers in Slaughtered Cattle and Relationship with Their Immunity Status at Ahvaz Abattoir. J Vet Res 63(2): 25-29.
Jaglic Z., Kucerova Z., Nedbalcova K., Kulich P., and Alexa P. 2006, Characterisation of Pasteurella multocida Isolated from Rabbits in the Czech Replublic. Veterinarni Medicina. 51(5):278-283.
Kartini D, Istiyaningsih, Maizi r A. 2009, Mutu Vaksin Septicaemia Epizootica yang Beredar di Indonesia Tahun 2007. Buletin Penguji Mutu Obat Hewan 14: 1-3.
Keputusan Menteri Pertanian Nomor 4026/Kpts./OT.140/3/2013 tentang Penetapan Jenis Penyakit Hewan Menular Strategis (PHMS). 10 Januari 2013. Kementerian Pertanian. Jakarta.
KPDE (Kantor Pengolahan Data Elektronik). 2006, Profil Daerah dan Potensi Peternakan Kota Kupang.
Kresno, B.S. 2010, Imunologi : Diagnosis dan Proses Laboratorium, Edisi Kelima, Jakarta : Balai Penerbit Fakultas Kedokteran Universitas Indonesia.
Munazir, Z. 2001, Respons Imun terhadap Infeksi Bakteri. Sari Pediatri, Vol. 2, No. 4, Maret 2001: 193 – 197.
Muneer, R., Hussain, M., Zahoor, A.B. 2005, Efficacy of Oil Based Haemorrhagic Septicaemia Vaccine: A Field Trial. Int. J. Agri. Biol. 7(4): 571-573.
Murtini, S. 2004, Tata Cara Pengambilan, Penggunaan dan Pemeriksaan Hewan dan Bahan Asal Hewan di Laboratorium. Badan Karantina Hewan Kementerian Pertanian.
Office International Des Epiooties (OIE). 2009, Haemorrhagic Septicaemia. The Center for Food Security & Public Health. Institute for International Cooperation in Animal Biologics, an OIE Collaborating Center: 1-5.
Peterson, S.N., Liu, H., Bergman, N.H., Shallom, S., Hazen, A., Crossno, J. Rasko, D.A., Ravel, J., Read, D.T., Yates, J. Hanna, P.C. 2004, Formation and Composition of Bacillus anthracis Endospore, Journal of Bacteriology American Society P.164-178.
Priadi, A., Natalia, L. 2000, Patogenesis Septicaemia epizootica (SE) pada Sapi/ Kerbau: Gejala Klinis, Perubahan Patologis, Reisolasi, Deteksi Pasteurella multocida dengan Kultur dan Polymerase Chain Reaction (PRC). Jurnal Ilmu Ternak dan Veteriner 5(1): 65-71.
Putra A.A G., Ekaputra I.G.M.A., Putra A.A.G.S., Dartini N.L. 2003, Surveilans Penyakit Ngorok di Pulau Sumba Provinsi Nusa Tenggara Timur Tahun 1994-1995. Buletin Veteriner BPPV Denpasar 15(62) : 15-21.
Putra A.A.G., Ekaputra I.G.M.A., Dartini N.L. 2003, Surveilans Zat Kebal Alami Dan Usaha Isolasi Pasteurella multocida pada Sapi Bali di Pulau Lombok. Buletin Veteriner BPPV Denpasar 15 (62) : 1-14.
Radji M. 2010, Imunologi Virologi. Penerbit PT. ISFI. Jakarta.
Setiawan I.M. 2007, Pemeriksaan Enzime-Linked Immunosorbent Assay (ELISA) untuk Diagnosis Leptospirosis. Jakarta Utara: Ebers Papyrus–vol. 13 No 3.
Shivachandra S.B., Nagaleekar V.K., Kumar A.A. 2011, A Review of Haemorrhagic Septicemia in Cattle and Buffalo.An H Res 12(1): 67-82.
Soeripto. 2002, Pendekatan Konsep Kesehatan Hewan melalui Vaksinasi, Jurnal Penelitian dan Pengembangan Pertanian 21:48-55.
Sugiyono. 2012, Metode Penelitian Administrasi. Cetakan ke-20. Penerbit Alfabeta. Bandung.
Suwarno. 2003, Prinsip Dasar Optimalisasi dan Interpretasi Hasil Uji ELISA, Lab Virologi dan Imunologi FKH Unair. Surabaya.
Tizard I.R. 2004, Veterinary Immunology an Introduction. 7th Ed. USA: Saunders.
Published
2020-06-19
How to Cite
H.Cantona, M., Sanam, M., Utami, T., Tophianong, T., & Widi, A. (2020). EVALUASI TITER ANTIBODI PASCA VAKSINASI Septicaemia epizootica PADA SAPI BALI DI KOTA KUPANG. JURNAL KAJIAN VETERINER, 8(1), 69-80. https://doi.org/10.35508/jkv.v8i1.2292
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Articles
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