STUDI MORFOMETRIK DAN MORFOLOGI SPERMATOZOA ENAM RUMPUN AYAM LOKAL INDONESIA
Study of Sperm Morphometric and Morphology of Six Indonesian Local Chicken Breeds
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Khaeruddin - Khaeruddin(1*)
Program Studi Peternakan, Fakultas Pertanian, Universitas Muhammadiyah Sinjai
https://orcid.org/0000-0001-5935-4602
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Hermawansyah Hermawansyah(2)
Program Studi Peternakan, Fakultas Pertanian, Universitas Muhammadiyah Sinjai
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Bahri Syamsuryadi(3)
Program Studi Peternakan, Fakultas Pertanian, Universitas Muhammadiyah Sinjai
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Junaedi Junaedi(4)
Bagian Peternakan, Fakultas Pertanian Perikanan dan Peternakan, Universitas Sembilanbelas November
(*) Corresponding Author
Keywords:
Morphometric, Morphology, Sperm, Indonesian local chicken
Abstract
This study aims to determine the morphometric comparison and identify abnormal sperm morphology in six types of Indonesian local chickens. Sperm observed came from six types of local chickens: red jungle fowl, Sentul chicken, Gaga’ chicken, Kampung Unggul Balitnak (KUB) chicken, Arab Silver chicken, and Bangkok chicken. Semen was collected by sequencing technique, then fresh semen was blotted on glass slides with eosin-nigrosine dye. Morphometrics and morphology of sperm were observed using a light microscope with a magnification of 100x16. ImageJ software was used for sperm morphometric observation on 60 sperm cells, while morphological observations were carried out by counting normal and abnormal sperm on 200 sperm cells. The results showed that there were variations in the size of sperm among the six types of chickens. Red jungle fowl had a higher total length and tail length than Sentul, Gaga', KUB, Arab, and Bangkok chickens. Observation of morphological characteristics showed that sperm with the highest abnormal morphology were in red jungle fowl and gaga' chickens. Tail bending and mid-piece bending is the most common abnormalities found in Indonesian local chickens.
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References
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Shanmugam M, Rajkumar U, Reddy M, Rao SR. 2012. Effect of Age on Semen Quality in Naked Neck and Dwarf Chicken Under Tropical Climatic Conditions. Animal Production Science 52: 964-568.
Tabatabaei S, Chaji M, Mohammadabadi T. 2010. Correlation Between Age of Rooster and Semen Quality in Iranian Indigenous Broiler Breeder Chickens. Journal of Animal and Veterinary Advances 9(1): 195-198.
Vidal AF, Colominas JA. 2007. Genetic Quality of Red Legged Partridge in Spain and Proposals for Its Conservation and Improvement. Selecciones Avícolas 10: 667–672.
Villaverde‑Morcillo S, Esteso MC, Castano C, Toledano Diaz A, Lopez‑Sebastian A, Campo JL, Santiago-Moreno J. 2015. Influence of Staining Method on the Values of Avian Sperm Head Morphometric Variables. Reproduction in Domestic Animals 50: 750–755.
Wysokińska A, Kondracki S. 2019. Heterosis for Morphometric Characteristics of Sperm Cells From Duroc x Pietrain Crossbred Boars. Animal Reproduction Science 211: 106217.
Almahdi AB, Ondho YS, Sutopo. 2014. Comparative Studies of Semen Quality on Different Breed of Chicken in Poultry Breeding Center Temanggung-Central Java. International Refereed Journal of Engineering and Science 3(2): 94-103.
Andraszek K, Banaszewska D, Biesiada-Drzazga B. 2018. The Use of Two Staining Methods for Identification of Spermatozoon Structure in Roosters. Poultry Science 97(7): 2575-2581.
Ardhani F, Raharja IMU, Boangmanalu BM, Handoko J. 2018. Karakteristik Morfologik dan Morfometrik Spermatozoa Ayam Nunukan. Jurnal Peternakan 15 (2): 62-67.
Birkhead T. 2000. Promiscuity: An Evolutionary History of Sperm Competition. London: Harvard University Press.
Briskie JV, Montgomerie R, Birkhead TR. 1997. The Evolution of Sperm Size in Birds. Evolution 51: 937–45.
Ervandi M. 2017. Motilitas dan Abnormalitas Bangsa Pejantan Setelah Penyimpanan. Akademika 6(2): 113-119.
Ervandi M, Ardiansya W, Prahara S. 2020. Kualitas dan Fertilitas Spermatozoa sebagai Akibat Pejantan Berbeda. Jambura Journal of Animal Science 2(2): 29-37.
Esteso MC, Fernandez‑Santos MR, Soler AJ, Garde JJ. 2003. Head Dimensions of Cryopreserved Red Deer Spermatozoa are Affected by Thawing Procedure. Cryo Letters 24: 261–268.
Esteso MC, Soler AJ, Fernandez‑Santos MR, Quintero‑Moreno AA, Garde JJ. 2006. Functional Significance of the Sperm Head Morphometric Size and Shape for Determining Freezability in Iberian red Deer (Cervus elaphus hispanicus) Epididymal Sperm Samples. Journal of Andrology 27: 662–670.
Feradis. (2010). Bioteknologi Reproduksi pada Ternak. Bandung: Alfabeta
Feyisa SG, Park YH, Kim YM, Lee BR, Jung KM, Choi SB, Cho CY, Han JY. 2018. Morphological Defects of Sperm and Their Association with Motility, Fertility, and Hatchability in Four Korean Native Chicken Breeds. Asian-Australasian Journal of Animal Science 31(8): 1160–1168.
Garcia‑Herreros M. 2016. Sperm Subpopulations in Avian Species: A Comparative Study Between the Rooster (Gallus domesticus) and Guinea fowl (Numida meleagris). Asian Journal of Andrology 18: 889-894.
Haunshi S, Niranjan M, Shanmugam M, Padhi MK, Reddy MR, Sunitha R, Rajkumar U, Panda AK. 2011. Characterization of Two Indian Native Chicken Breeds for Production, Egg and Semen Quality, and Welfare Traits. Poultry Science 90: 314-320.
Hambu EK, Arifiantini RI, Purwantara B, Darwati S. 2016. Raw Semen Characteristics of Three Different Indonesian Local Roosters. Animal Production 18(3): 165-172.
Hardijanto, Sardjito T, Hernawati T, Susilowati S, Suprayogi TW. 2010. Buku Ajar Inseminasi Buatan. Surabaya: Airlangga University Press.
Immler S, Saint‑Jalme M, Lesobre L, Sorci G, Roman Y, Birkhead TR. 2007. The evolution of sperm morphometry in pheasants. Journal of Evolutionary Biology 20: 1008–1014.
Khaeruddin, Arismunandar, Nurda. 2020. Karakteristik Semen Ayam Kampung Yang Diberi Minyak Hati Ikan Kod Sebagai Feed Suplement. Musamus Journal of Livestock Science 3(1): 15-24.
Kleven O, Fossoy F, Laskemoen T, Robertson RJ, Rudolfsen G, Lifjedl JT. 2009. Comparative Evidence for the Evolution of Sperm Swimming Speed by Sperm Competition and Female Sperm Storage Duration in Passerine Birds. Evolution 63: 2466–2473.
Łukaszewicz E, Jerysz A, Partyka A, Siudzinska A. 2008. Efficacy of Evaluation of Rooster Sperm Morphology Using Different Staining Methods. Research in Veterinary Science 85: 583-388.
Maroto‑Morales A, Ramon M, Garcia‑Alvarez O, Soler AJ, Fernandez‑Santos MR, Roldan ERS, Gomendio M, Pérez-Guzmán MD, Garde JJ. 2012. Morphometrically‑Distinct Sperm Subpopulations Defined by a Multistep Statistical Procedure in Ram Ejaculates: Intra‑ and Interindividual Variation. Theriogenology 77: 1529–1539.
Mugiyono S, Saleh DM, Sukardi. 2015. Reproductive Performance of Various Breeds of Sentul Chicken. Animal Production 17 (3): 169-176.
Nataamijaya AG. 2010. Pengembangan Potensi Ayam Lokal Untuk Menunjang Peningkatan Kesejahteraan Petani. Jurnal Litbang Pertanian 29(4): 131-138.
Peña FJ, Saravia F, García‑Herreros M, Núñez‑Martinez I, Tapia JA, Johannison A, Wallgren M, Rodríguez-Martínez H. 2005. Identification of Sperm Morphometric Subpopulations in Two Different Portions of the Boar Ejaculate and Its Relation to Post‑Thaw Quality. Journal of Andrology 26: 716–723.
Rakha BA, Ansari MS, Hussain I, Malik MF, Akhter S, Blesbois E. (2015). Semen Characteristics of the Indian Red Jungle Fowl (Gallus gallus murghi). European Journal of Wildlife Research. 61, 3, 379-386.
Santiago‑Moreno J, Castaño C, Toledano‑Díaz A, Esteso MC, López‑Sebastían A, Gañán N, Hierro MJ, Marchal F, Campo JL, Blesbois E. 2015. Characterization of red‑legged partridge (Alectoris rufa) sperm: seasonal changes and influence of genetic purity. Poultry Science 94: 80–87.
Santiago‑Moreno J, Esteso MC, Villaverde‑Morcillo S, Toledano‑Diaz A, Castaño C, Velázquez R, Lopez‑Sebastian A, Goya AL, Martínez JG. 2016. Recent Advances in Bird Sperm Morphometric Analysis and Its Role in Male Gamete Characterization and Reproduction Technologies. Asian Journal of Andrology 18: 1-7.
Shanmugam M, Rajkumar U, Reddy M, Rao SR. 2012. Effect of Age on Semen Quality in Naked Neck and Dwarf Chicken Under Tropical Climatic Conditions. Animal Production Science 52: 964-568.
Tabatabaei S, Chaji M, Mohammadabadi T. 2010. Correlation Between Age of Rooster and Semen Quality in Iranian Indigenous Broiler Breeder Chickens. Journal of Animal and Veterinary Advances 9(1): 195-198.
Vidal AF, Colominas JA. 2007. Genetic Quality of Red Legged Partridge in Spain and Proposals for Its Conservation and Improvement. Selecciones Avícolas 10: 667–672.
Villaverde‑Morcillo S, Esteso MC, Castano C, Toledano Diaz A, Lopez‑Sebastian A, Campo JL, Santiago-Moreno J. 2015. Influence of Staining Method on the Values of Avian Sperm Head Morphometric Variables. Reproduction in Domestic Animals 50: 750–755.
Wysokińska A, Kondracki S. 2019. Heterosis for Morphometric Characteristics of Sperm Cells From Duroc x Pietrain Crossbred Boars. Animal Reproduction Science 211: 106217.
Published
2021-11-21
How to Cite
Khaeruddin, K., Hermawansyah, H., Syamsuryadi, B., & Junaedi, J. (2021). STUDI MORFOMETRIK DAN MORFOLOGI SPERMATOZOA ENAM RUMPUN AYAM LOKAL INDONESIA. JURNAL KAJIAN VETERINER, 9(3), 123-134. https://doi.org/10.35508/jkv.v9i3.5171
Section
Articles
Copyright (c) 2021 JURNAL KAJIAN VETERINER
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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