Analisis Nukleotida dan Homologi Sekuens Fragmen Gen p72 (B646L) Virus African Swine Fever Virus (ASF) Asal Kota Kupang
Nucleotide and Homology Analysis of the p72 Gene Fragment of African Swine Fever Virus (ASFV) from Kupang City
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Maxs U. E. Sanam(1)
Laboratorium Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang
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Maria Aega Gelolodo(2*)
https://orcid.org/0000-0002-8912-5779
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Larry R. W. Toha(3)
Laboratorium Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang
(*) Corresponding Author
Keywords:
African Swine Fever, ASF, Kupang, Pig, Timor, Virus
Abstract
African Swine Fever (ASF) is an important infectious disease in pigs caused by African Swine Fever Virus (ASFV). Despite not being zoonotic, this disease has the potential to severely affect the socioeconomic conditions in the impacted regions. The majority of pig farmers in Indonesia, particularly those in Kupang City, that raise pigs in backyards or on a small scale, experience the impact of ASF's effects. Early in 2020, the ASF cases were confirmed in Timor Island, including the Kupang City area in Nusa Tenggara Timur (NTT) Province. The molecular information on ASFV in this area is still limited. In order to determine the homology and nucleotide analysis using BLAST NCBI, the ASFV p72 (B646L) gene fragment sequence from Kupang City was compared to ASFV p72 (B646L) gene segments from other parts of Indonesia and several other Asian countries. The results of nucleotide analysis and sequence homology of the original ASFV p72 (B646L) gene fragment from Kupang City showed a high level of homology to the ASFV p72 (B646L) gene fragment from West Java, North Sumatra, and several Asian countries. The findings from this study indicate that the source of ASF viral transmission across different regions may be comparable. Therefore, to prevent the dissemination of ASF, strict biosecurity measures must be implemented along with monitoring of animal and product transportation.
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References
Achenbach, J. E., Gallardo, C., Nieto-Pelegrin, E., Rivera-Arroyo, B., Degefa-Negi, T., Arias, M., . . . Sanchez-Vizcaino, J. M. (2017). Identification of a New Genotype of African Swine Fever Virus in Domestic Pigs from Ethiopia. Transbound Emerg Dis, 64(5), 1393-1404. doi:10.1111/tbed.12511
Alejo, A., Matamoros, T., Guerra, M., & Andrés, G. (2018). A Proteomic Atlas of the African Swine Fever Virus Particle. Journal of Virology, 92(23), e01293-01218. doi:10.1128/JVI.01293-18
Alonso, C., Borca, M., Dixon, L., Revilla, Y., Rodriguez, F., Escribano, J. M., & Consortium, I. R. (2018). ICTV Virus Taxonomy Profile: Asfarviridae. J Gen Virol, 99(5), 613-614. doi:10.1099/jgv.0.001049
Andrés, G., Charro, D., Matamoros, T., Dillard, R. S., & Abrescia, N. G. A. (2020). The cryo-EM structure of African swine fever virus unravels a unique architecture comprising two icosahedral protein capsids and two lipoprotein membranes. Journal of Biological Chemistry, 295(1), 1-12. doi:https://doi.org/10.1074/jbc.AC119.011196
Bastos, A. D., Penrith, M. L., Cruciere, C., Edrich, J. L., Hutchings, G., Roger, F., . . . G, R. T. (2003). Genotyping field strains of African swine fever virus by partial p72 gene characterisation. Arch Virol, 148(4), 693-706. doi:10.1007/s00705-002-0946-8
Beltrán-Alcrudo, D., Arias, M., Gallardo, C., Kramer, S. A., & Penrith, M.-L. (Eds.). (2017). African swine fever: detection and diagnosis – A manual for veterinarians (FAO Animal Production and Health Manual No. 19 ed.): Food and Agriculture Organization of the United Nations (FAO).
Bishop, R. P., Fleischauer, C., de Villiers, E. P., Okoth, E. A., Arias, M., Gallardo, C., & Upton, C. (2015). Comparative analysis of the complete genome sequences of Kenyan African swine fever virus isolates within p72 genotypes IX and X. Virus Genes, 50(2), 303-309. doi:10.1007/s11262-014-1156-7
Blome, S., Franzke, K., & Beer, M. (2020). African swine fever - A review of current knowledge. Virus Res, 287, 198099. doi:10.1016/j.virusres.2020.198099
Blome, S., Gabriel, C., & Beer, M. (2013). Pathogenesis of African swine fever in domestic pigs and European wild boar. Virus Research, 173(1), 122-130. doi:https://doi.org/10.1016/j.virusres.2012.10.026
Costard, S., Mur, L., Lubroth, J., Sanchez-Vizcaino, J. M., & Pfeiffer, D. U. (2013). Epidemiology of African swine fever virus. Virus Research, 173(1), 191-197. doi:https://doi.org/10.1016/j.virusres.2012.10.030
Dharmayanti, N. I., Sendow, I., Ratnawati, A., Settypalli, T. B. K., Saepulloh, M., Dundon, W. G., . . . Lamien, C. E. (2021). African swine fever in North Sumatra and West Java provinces in 2019 and 2020, Indonesia. Transbound Emerg Dis. doi:10.1111/tbed.14070
Dixon, L. K., Chapman, D. A. G., Netherton, C. L., & Upton, C. (2013). African swine fever virus replication and genomics. Virus Research, 173(1), 3-14. doi:https://doi.org/10.1016/j.virusres.2012.10.020
Dixon, L. K., Sun, H., & Roberts, H. (2019). African swine fever. Antiviral Res, 165, 34-41. doi:10.1016/j.antiviral.2019.02.018
Galindo, I., & Alonso, C. (2017). African Swine Fever Virus: A Review. Viruses, 9(5). doi:10.3390/v9050103
Gallardo, C., Sastre, P., Rueda, P., Gerilovych, A. P., Kit, M., Nurmoja, I., & Potier, M. F. L. (2021). 5. Methods for African swine fever diagnosis in clinical and environmental samples. In Understanding and combatting African Swine Fever (pp. 141-160): Wageningen Academic Publishers.
Izzati, U. Z., Inanaga, M., Hoa, N. T., Nueangphuet, P., Myint, O., Truong, Q. L., . . . Yamaguchi, R. (2020). Pathological investigation and viral antigen distribution of emerging African swine fever in Vietnam. Transboundary and Emerging Diseases.
Jia, N., Ou, Y., Pejsak, Z., Zhang, Y., & Zhang, J. (2017). Roles of African Swine Fever Virus Structural Proteins in Viral Infection. Journal of veterinary research, 61(2), 135-143. doi:10.1515/jvetres-2017-0017
Njau, E. P., Domelevo Entfellner, J. B., Machuka, E. M., Bochere, E. N., Cleaveland, S., Shirima, G. M., . . . Okoth, E. A. (2021). The first genotype II African swine fever virus isolated in Africa provides insight into the current Eurasian pandemic. Sci Rep, 11(1), 13081. doi:10.1038/s41598-021-92593-2
OIE. (2021a). African Swine Fever (Infection with African Swine Fever Virus). In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (pp. 1-18).
OIE. (2021b). Follow-up report 3 African swine fever virus (Inf. with) , Indonesia. Retrieved from
https://wahis.oie.int/#/report-info?reportId=28198
OIE. (2021c). Situational updates of ASF in Asia and the Pacific. Retrieved from https://rr-asia.oie.int/en/projects/asf/situational-updates-of-asf/. https://rr-asia.oie.int/en/projects/asf/situational-updates-of-asf/
Penrith, M. L., Bastos, A. D., Etter, E. M. C., & Beltrán-Alcrudo, D. (2019). Epidemiology of African swine fever in Africa today: Sylvatic cycle versus socio-economic imperatives. Transbound Emerg Dis, 66(2), 672-686. doi:10.1111/tbed.13117
Portugal, R., Coelho, J., Hoper, D., Little, N. S., Smithson, C., Upton, C., . . . Keil, G. M. (2015). Related strains of African swine fever virus with different virulence: genome comparison and analysis. J Gen Virol, 96(Pt 2), 408-419. doi:10.1099/vir.0.070508-0
Rajkhowa, T. K., Kiran, J., Hauhnar, L., Zodinpui, D., Paul, A., & Sagolsem, S. (2022). Molecular detection and characterization of African Swine Fever irus from field outbreaks in domestic pigs, Mizoram, India. Transbound Emerg Dis, 69(4), e1028-e1036. doi:10.1111/tbed.14384
Salguero, F. J. (2020). Comparative Pathology and Pathogenesis of African Swine Fever Infection in Swine. Frontiers in veterinary science, 7(282). doi:10.3389/fvets.2020.00282
Sanchez-Cordon, P. J., Montoya, M., Reis, A. L., & Dixon, L. K. (2018). African Swine fever: A re-emerging Viral Disease Threatening the Global Pig Industry. Vet J, 233, 41-48. doi:10.1016/j.tvjl.2017.12.025
Sendow, I., Ratnawati, A., Dharmayanti, N. L. P., & Saepulloh, M. (2020). African Swine Fever: Penyakit Emerging yang Mengancam Peternakan Babi di Dunia. Indonesian Bulletin of Animal and Veterinary Sciences, 30, 15. doi:10.14334/wartazoa.v30i1.2479
Sun, E., Huang, L., Zhang, X., Zhang, J., Shen, D., Zhang, Z., . . . Bu, Z. (2021). Genotype I African Swine Fever Viruses Emerged in Domestic Pigs in China and Caused Chronic infection. Emerging Microbes & Infections, 10(1), 2183-2193. doi:10.1080/22221751.2021.1999779.
Alejo, A., Matamoros, T., Guerra, M., & Andrés, G. (2018). A Proteomic Atlas of the African Swine Fever Virus Particle. Journal of Virology, 92(23), e01293-01218. doi:10.1128/JVI.01293-18
Alonso, C., Borca, M., Dixon, L., Revilla, Y., Rodriguez, F., Escribano, J. M., & Consortium, I. R. (2018). ICTV Virus Taxonomy Profile: Asfarviridae. J Gen Virol, 99(5), 613-614. doi:10.1099/jgv.0.001049
Andrés, G., Charro, D., Matamoros, T., Dillard, R. S., & Abrescia, N. G. A. (2020). The cryo-EM structure of African swine fever virus unravels a unique architecture comprising two icosahedral protein capsids and two lipoprotein membranes. Journal of Biological Chemistry, 295(1), 1-12. doi:https://doi.org/10.1074/jbc.AC119.011196
Bastos, A. D., Penrith, M. L., Cruciere, C., Edrich, J. L., Hutchings, G., Roger, F., . . . G, R. T. (2003). Genotyping field strains of African swine fever virus by partial p72 gene characterisation. Arch Virol, 148(4), 693-706. doi:10.1007/s00705-002-0946-8
Beltrán-Alcrudo, D., Arias, M., Gallardo, C., Kramer, S. A., & Penrith, M.-L. (Eds.). (2017). African swine fever: detection and diagnosis – A manual for veterinarians (FAO Animal Production and Health Manual No. 19 ed.): Food and Agriculture Organization of the United Nations (FAO).
Bishop, R. P., Fleischauer, C., de Villiers, E. P., Okoth, E. A., Arias, M., Gallardo, C., & Upton, C. (2015). Comparative analysis of the complete genome sequences of Kenyan African swine fever virus isolates within p72 genotypes IX and X. Virus Genes, 50(2), 303-309. doi:10.1007/s11262-014-1156-7
Blome, S., Franzke, K., & Beer, M. (2020). African swine fever - A review of current knowledge. Virus Res, 287, 198099. doi:10.1016/j.virusres.2020.198099
Blome, S., Gabriel, C., & Beer, M. (2013). Pathogenesis of African swine fever in domestic pigs and European wild boar. Virus Research, 173(1), 122-130. doi:https://doi.org/10.1016/j.virusres.2012.10.026
Costard, S., Mur, L., Lubroth, J., Sanchez-Vizcaino, J. M., & Pfeiffer, D. U. (2013). Epidemiology of African swine fever virus. Virus Research, 173(1), 191-197. doi:https://doi.org/10.1016/j.virusres.2012.10.030
Dharmayanti, N. I., Sendow, I., Ratnawati, A., Settypalli, T. B. K., Saepulloh, M., Dundon, W. G., . . . Lamien, C. E. (2021). African swine fever in North Sumatra and West Java provinces in 2019 and 2020, Indonesia. Transbound Emerg Dis. doi:10.1111/tbed.14070
Dixon, L. K., Chapman, D. A. G., Netherton, C. L., & Upton, C. (2013). African swine fever virus replication and genomics. Virus Research, 173(1), 3-14. doi:https://doi.org/10.1016/j.virusres.2012.10.020
Dixon, L. K., Sun, H., & Roberts, H. (2019). African swine fever. Antiviral Res, 165, 34-41. doi:10.1016/j.antiviral.2019.02.018
Galindo, I., & Alonso, C. (2017). African Swine Fever Virus: A Review. Viruses, 9(5). doi:10.3390/v9050103
Gallardo, C., Sastre, P., Rueda, P., Gerilovych, A. P., Kit, M., Nurmoja, I., & Potier, M. F. L. (2021). 5. Methods for African swine fever diagnosis in clinical and environmental samples. In Understanding and combatting African Swine Fever (pp. 141-160): Wageningen Academic Publishers.
Izzati, U. Z., Inanaga, M., Hoa, N. T., Nueangphuet, P., Myint, O., Truong, Q. L., . . . Yamaguchi, R. (2020). Pathological investigation and viral antigen distribution of emerging African swine fever in Vietnam. Transboundary and Emerging Diseases.
Jia, N., Ou, Y., Pejsak, Z., Zhang, Y., & Zhang, J. (2017). Roles of African Swine Fever Virus Structural Proteins in Viral Infection. Journal of veterinary research, 61(2), 135-143. doi:10.1515/jvetres-2017-0017
Njau, E. P., Domelevo Entfellner, J. B., Machuka, E. M., Bochere, E. N., Cleaveland, S., Shirima, G. M., . . . Okoth, E. A. (2021). The first genotype II African swine fever virus isolated in Africa provides insight into the current Eurasian pandemic. Sci Rep, 11(1), 13081. doi:10.1038/s41598-021-92593-2
OIE. (2021a). African Swine Fever (Infection with African Swine Fever Virus). In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (pp. 1-18).
OIE. (2021b). Follow-up report 3 African swine fever virus (Inf. with) , Indonesia. Retrieved from
https://wahis.oie.int/#/report-info?reportId=28198
OIE. (2021c). Situational updates of ASF in Asia and the Pacific. Retrieved from https://rr-asia.oie.int/en/projects/asf/situational-updates-of-asf/. https://rr-asia.oie.int/en/projects/asf/situational-updates-of-asf/
Penrith, M. L., Bastos, A. D., Etter, E. M. C., & Beltrán-Alcrudo, D. (2019). Epidemiology of African swine fever in Africa today: Sylvatic cycle versus socio-economic imperatives. Transbound Emerg Dis, 66(2), 672-686. doi:10.1111/tbed.13117
Portugal, R., Coelho, J., Hoper, D., Little, N. S., Smithson, C., Upton, C., . . . Keil, G. M. (2015). Related strains of African swine fever virus with different virulence: genome comparison and analysis. J Gen Virol, 96(Pt 2), 408-419. doi:10.1099/vir.0.070508-0
Rajkhowa, T. K., Kiran, J., Hauhnar, L., Zodinpui, D., Paul, A., & Sagolsem, S. (2022). Molecular detection and characterization of African Swine Fever irus from field outbreaks in domestic pigs, Mizoram, India. Transbound Emerg Dis, 69(4), e1028-e1036. doi:10.1111/tbed.14384
Salguero, F. J. (2020). Comparative Pathology and Pathogenesis of African Swine Fever Infection in Swine. Frontiers in veterinary science, 7(282). doi:10.3389/fvets.2020.00282
Sanchez-Cordon, P. J., Montoya, M., Reis, A. L., & Dixon, L. K. (2018). African Swine fever: A re-emerging Viral Disease Threatening the Global Pig Industry. Vet J, 233, 41-48. doi:10.1016/j.tvjl.2017.12.025
Sendow, I., Ratnawati, A., Dharmayanti, N. L. P., & Saepulloh, M. (2020). African Swine Fever: Penyakit Emerging yang Mengancam Peternakan Babi di Dunia. Indonesian Bulletin of Animal and Veterinary Sciences, 30, 15. doi:10.14334/wartazoa.v30i1.2479
Sun, E., Huang, L., Zhang, X., Zhang, J., Shen, D., Zhang, Z., . . . Bu, Z. (2021). Genotype I African Swine Fever Viruses Emerged in Domestic Pigs in China and Caused Chronic infection. Emerging Microbes & Infections, 10(1), 2183-2193. doi:10.1080/22221751.2021.1999779.
Published
2022-12-19
How to Cite
Sanam, M., Gelolodo, M., & Toha, L. (2022). Analisis Nukleotida dan Homologi Sekuens Fragmen Gen p72 (B646L) Virus African Swine Fever Virus (ASF) Asal Kota Kupang. JURNAL KAJIAN VETERINER, 10(2), 165-175. https://doi.org/10.35508/jkv.v10i2.7875
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Articles
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