JURNAL KAJIAN VETERINER

Studi Morfologi Kelenjar Aksesoris Kelamin Jantan Sapi Sumba Ongole (Bos indicus)

Angelica Noni Togola — 2024-06-18

Sumba Ongole cattle are one of the local types of cattle that are most commonly found in Indonesia. Bulls have accessory gland are ampulla, vesicular glands, prostate glands, and bulbourethral glands. The aim of this research is to determine the anatomical morphology and histology of male accessory glands in Sumba Ongole cattle. The research sample was six Sumba Ongole cattle that had been slaughtered at the East Sumba Regency Slaughterhouse. Macroscopic examination carried out tissue fixation in 10% formalin, tissue processing, and hematoxylin-eosin staining. The results of the research show that the male accessory glands in Sumba Ongole cattle have the ampullary gland, vesicular gland, prostate gland and bulbourethral gland.. Microscopically, each gland is covered by a capsule and has a secretory gland. The ampullary secretory glands are lined by cylindrical epithelium and have a serous type. The secretory glands of the vesicular glands are lined with cylindrical epithelium and have a seromucous type, the secretory glands of the prostate gland are lined with cuboidal epithelium and have a seromucous type, and the secretory glands of the bulbourethral glands are lined by cuboidal epithelium and have the mucous type.

Studi Kasus: Terapi Mastitis Klinis pada Sapi Perah di Kota Batu

Intan Permatasari Hermawan — 2024-06-18

The purpose of this case report is to report a case of clinical mastitis in dairy cattle in Batu. One case was reported on this period. Anamnesis was carried out, the owner revealed that the cow did not produce milk and when milking the udderand nipples felt hard and the milk that wanted to come out seemed to be stuck. Physical examination was carried out, cow’s weight ±400 kg, body temperature 38.4 °C, the udder and nipple were swollen and inflamed, and decrease of milk production, mammae showed reddish and asymmetrical and painful when palpate. Based on the history and physical examination of the cow, it can be concluded that the cow was diagnosed with clinical mastitis with a fausta prognosis and the differential diagnosis was mammary tumour. The therapy given in this case was injection of the antibioticInterspectin-L® 10 ml, the anti-inflammatory and antipyretic Tolfedine® 10 ml intramuscularly.

Kualitas Daging Se’i Babi melalui Aplikasi Tepung Rosela

Veronika Matutina — 2024-06-20

Se'i meat is a typical meat from East Nusa Tenggara which is made by smoking it. The meat is sliced in small lengths, then smoked until cooked and has a distinctive taste. This study aims to determine the effect of rosella flour (Hibiscus sabdariffa L.) to organoleptic (colour, aroma, taste and tenderness); pH; TPC and E. coli pork. The research method uses a Complete Randomized Design (RAL) consisting of 4 treatments and 4 repetitions. The treatment consisted of P0: pork se’i meat without the addition of rosella flour, P1: pork se’i meat with the addition of rosella flour 0.5%, P2: pork se’i meat with the addition of rosella flour 1%, P3: pork se’i meat with the addition of rosella flour 1.5%. The organoleptic test of colour, aroma, taste and tenderness of pork meat was analysed using the Kruskal Wallis test followed by the Mann Whitney test, while the pH, TPC and E. Coli. analysed using ANOVA followed by Duncan's test to see any differences between treatments. The results showed that the application of roselle flour to pork se'i meat with different concentrations (0.5%, 1% and 1.5%) affected the organoleptic quality (colour, taste and tenderness) but did not affect the aroma of pork se'i meat. The addition of roselle flour can also lower the pH, TPC and E. coli pork. It is concluded that the higher the amount of rosella flour shows good results for the quality of pig meat because it increases the taste and tenderness and lowers the pH, TPC and E.coli of pork se’i.

Identifikasi Mikroplastik pada Ikan Tongkol Lisong (Auxis Rochei) dan Ikan Tuna Makarel (Euthynnus Affinis) di Pangkalan Pendaratan Ikan (PPI) Oeba, Kupang

Gendhis Larasati — 2024-06-22

Waste management in Kupang City is still very poor. The waste will eventually reach the sea and will be degraded into microplastics. Microplastics, which are less than 5 millimetres in size, are made from plastic waste in marine waters through physical, mechanical, chemical and biological processes. This research was conducted to determine the characteristics (shape and colour) and abundance of microplastics found in digestive tract samples, and meat/muscle samples of lisong tuna (Auxis rochei) and mackerel tuna (Euthynnus affinis) obtained from PPI Oeba, Kupang City. Each organ was extracted using 10% KOH for 48-72 hours and microplastic characteristics were observed visually using a stereo microscope. The research results found microplastics in the digestive tract and meat of A. rochei and E. affinis. The forms found in digestive tract A. rochei include films and fragments, with transparent colours. Meanwhile in meat, fragments, films and fibres were found in red, blue, black, transparent and purple. The forms found in digestive tract E. affinis include fragments, pellets, fibers and films with blue, black, transparent and yellow colours. Meanwhile in meat, fragments and pellets were found with black and yellow colors. The abundance of microplastics detected in digestive tract A. rochei included 1.2 MP/individual, while in meat it was 0.2 MP/gr. In E. affinis it includes 0.8 MP/individual in digestive tract and in meat 0.06 MP/gr.

Evaluasi Kerusakan DNA Spermatozoa Semen Cair Babi Menggunakan Pewarnaan Toluidine Blue

Hermilinda Parera — 2024-06-22

The integrity of the deoxyribonucleic acid (DNA) in sperm plays a crucial role in the fertilization process and embryonic development. This study aimed to evaluate the level of the DNA fragmentation of boar sperm in tris-citrate-fructose extender preserved at temperatures between 16-18°C for 3 days. The level of sperm DNA damage was analysed using toluidine blue (TB) staining. Pig semen collected from 4 male pigs aged 1.3-1.8 years was used as the research sample. Semen collected was conducted twice a week. The experimental method using a Completely Randomized Design with 4 storage duration groups as follows: Group I (0 hours of storage), Group II (24 hours of storage), Group III (48 hours of storage), and Group IV (72 hours of storage), each with 5 replications. The data were analysed using analysis of variance (ANOVA) and, if significant differences were found, Duncan's test was performed. The results of the evaluation of the percentage of DNA damage during the storage process at 16-18⁰C at 0 hours, 24 hours, 48 hours and 72 hours was: 0.33 ± 1.66%, 0.80 ± 0.447%, 1.60 ± 1.14% and 2.80 ± 0.447%, respectively. These results demonstrate that liquid boar semen preserved with tris citrate fructose extender at 16-18 ^°C for 3 days has spermatozoa DNA damage levels within the normal range, making it suitable for artificial insemination and ensuring optimal AI success.

Gambaran Hematologi dari Pemberian Anestesi Kombinasi Ketamin-Xilazin dan Ketamin-Diazepam pada Pelaksanaan Kastrasi Anjing

Tri Utami — 2024-06-19

Carrying out castration on dogs requires anesthesia to prevent movement and relieve pain during the operation. The aim of this study was to determine the effect of administering two combinations of general anesthesia, ketamine - xylazine and ketamine-diazepam on the blood features of dogs undergoing castration procedures. The dogs used in this study were 6 dogs, domestic breed, male and aged between six months and one year. Blood sampling from the cephalic antebrachii vein of 1 ml was carried out twice, thirty minutes before surgery and sixty minutes after surgery. The average results of post-operative complete blood tests in dogs given ketamine (10 mg/kg body weight, iv) – xylazine (1 mg/kg body weight, iv) anesthesia, include: total erythrocyte count 5.84 x 10⁶/µL, haemoglobin 12.27 g/dL, haematocrit 36.7%, and leukocytes 15.4 x 10³/µL. The average results of post-operative complete blood tests in dogs given ketamine (10 mg/kg body weight, iv)–diazepam (0.2 mg/kg body wight, iv) anesthesia, include: total erythrocyte count 5.98 x 10⁶/µL, haemoglobin 11.77 g/dL, haematocrit 36.23%, and leukocytes 16.4 x 10³/µL. Based on the results of statistical analysis using the independent t-test, the number of erythrocytes, haemoglobin, haematocrit and post-operative leukocytes between the two groups was not significantly different (P>0.05). The use of the combination of ketamine-xylazine and ketamine - diazepam during castration did not cause changes in the number of erythrocytes, haemoglobin, haematocrit and leukocytes.

Profile of Antibiotic Residue and Antibiotic Resistance in Broiler Chicken Meat in Indonesia: Public Health Importance

Maria Antonia Yersi Dua Bura — 2024-06-23

Keamanan pangan merupakan isu global dan menjadi perhatian bersama. Di bidang peternakan, penyalahgunaan antibiotik dalam praktik kesehatan hewan berdampak pada bahaya residu dan resistensi antimikroba pada produk pangan asal hewan bagi kesehatan masyarakat. Penelitian ini mengkaji profil residu dan resistensi antibiotik pada daging ayam broiler di Indonesia. Kajian dilakukan melalui tinjauan pustaka terhadap artikel penelitian yang dipublikasikan di jurnal ilmiah nasional dan internasional, prosiding seminar, dan laporan penelitian. Hasil penelitian mengungkapkan, dalam lima tahun terakhir (2018-2023) masih ditemukan residu antibiotik pada daging ayam broiler di beberapa daerah di Indonesia. Pada periode yang sama, resistensi antibiotik juga ditemukan pada sampel daging ayam broiler di beberapa wilayah Indonesia. Resistensi antibiotik yang ditemukan adalah pada satu jenis antibiotik dan beberapa jenis antibiotik (multidrug resistance) sekaligus. Temuan ini dapat dijadikan bahan evaluasi perbaikan manajemen rantai produksi dan distribusi produk daging ayam broiler dalam negeri tentang jaminan keamanan pangan asal hewan.

Analisis Semen Babi Yorkshire dalam Modifikasi Extender Alami Buah Lontar dalam Filtrat Jambu Air Hijau (Syzygiumaqueum)

Nancy Foeh — 2024-06-23

This study aims to see the effectiveness of Yorkshire boar semen natural modification of the natural diluent of green guava filtrate (Syzygiumaqueum). Semen is stored per 72 hours from a 3-year-old sow. Macro and microscopic evaluation of semen includes Volume, Colour, Consistency, pH, Odor and motility, concentration, viability, abnormality and normality of spermatozoa. The semen was diluted with palm fruit juice and added green guava filtrate (Syzygiumaqueum) in tiers: 100μL, 200μL, 300μL, 400 μL and 500 μL. The results showed that the modification of palm fruit juice diluent with the addition of 200 μL of green guava filtrate (Syzygiumaqueum) in Yorkshire boar semen was able to maintain the quality of spermatozoa up to 22 hours of storage.

Analisis Metabolit Sekunder Daun Brucea javanica [L] Merr dari Dua Tempat Berbeda di Pulau Timor

Julita Dewitri Mertha Yasa — 2024-06-20

The leaves of Brucea javanica [L] Merr have long been utilized as an alternative medicine for a variety of illness. Although a number of investigations have been conducted to ascertain the advantages and composition of secondary metabolites in this plant's leaves, nothing has ever been published on the particular study conducted on the island of Timor. The purpose of this study was to ascertain which secondary metabolites were present in the leaves of this plant that was grown in two distinct locations on Timor Island. The findings of this study will be used as an empirical basis for future investigations into the use of herbal remedies. The study's findings demonstrated that while flavonoids and alkaloids weren't present from the plant's ethanol extract, it did contain highly concentrated terpenoids and tannins at both locations. In contrast, saponin content was only discovered in leaves collected from the neighbourhood of Fatumnutu. The spectrophotometer test revealed that the ethanol extract of Bruce planta javanica [L] Merr from Noelbaki Village had a greater secondary metabolite absorption value compared to plant extract from Fatumnutu Village. The absorption values for tannin and alkaloid showed the largest overall variation in absorption levels, varying by around 0.513% and 0.68% for each constituent. In the meantime, there is a 0.513% variation in tannin compounds between the two sites. In order to ascertain the bioactivity of this plant's leaf extract for the creation of herbal medicines in Indonesia, more investigation is required into the variations in saponin levels and absorbency levels of secondary metabolite compounds from the two locations.

Struktur Populasi Ternak Sapi Bali di Pulau Semau

Firgilius Arifandi — 2024-06-24

Semau Island is an island across the west of the island of Timor which is an area of Kupang Regency with the potential for developing Bali cattle. This study aims to determine the population structure of Bali cattle in Semau Island. This research uses simple random sampling method. Collecting data in the form of primary data (interviews/questionnaires) and secondary data. The total sample used is 110 farmers. The parameters measured in the population structure are livestock birth rates, livestock purchases, livestock mortality, livestock slaughter, livestock sales, livestock income, livestock expenditure, and natural increase values. The results showed that the population structure of Bali cattle on Semau Island which was owned by the respondents was dominated by 455 female cows with a total of 1103 cows. Birth rate of 16.59%, purchase rate of 4.26%, death rate of 4.17%, slaughter rate of 0.18%, sales rate of 10.06%, income rate of 20.85%, expenditure rate of 14.42%, and the Natural Increase (NI) value of 12.42%.

Kurkumin sebagai Agen Penghambat Penurunan Bobot Badan dan Skleroderma pada Mencit Akibat Induksi Bleomisin

Annisa Rahmi — 2024-06-28

Curcumin is the main active ingredient from turmeric plant (Curcuma longa) has been known as anti-oxidant and anti-inflammatory agent. Bleomycin is an anti-cancer drug which have side effects such as scleroderma and body weight loss in animals or human. This study was aimed to determined biological effects of curcumin to bleomycin-induced scleroderma and body weight loss in mice (Mus musculus). Sixteen male mice (ddy strain) were devided in to four groups: (i) negative control, received aquadest injection 0,1 ml (SC); (ii) bleomycin (BLM), received 0,1 ml bleomycin 1 mg/ml SC injection; (iii) curcumin (CMN), received aquadest injection 0,1 ml (SC) and 100 mg/kg BW curcumin in 0,5% carboxymethylcellulose (IP); and (iv) BLM+ CMN, received 0,1 ml bleomycin 1 mg/ml SC injection and 100 mg/kg BW curcumin in 0,5% carboxymethylcellulose (IP). All treatments are given every day for 4 weeks and body weigh were measured every week. The dorsal skin as injection’s dot evaluated by histopathology assessment with HE stains. BLM group showed body weight and hair follicles were loss significantly with skin fibrosis and inflammatory cells infiltration. However, CMN treatment showed opposite results in BLM-treated mice. Conclusively, this study showed CMN treatment may inhibit skin fibrogenesis in BLM-induced scleroderma and body weight loss.

Aktivitas Antioksidan dan Antibakteri Ekstrak Etanol Kulit Batang Lunasia amara

Agus Saputra — 2024-06-28

Nowadays, there is an increasing demand for medicines to treat many types of diseases. Lunasia amara is one of the medicinal plants used by Indonesian people for a long time. This study aimed to identify the secondary metabolites of L. amara stem bark using phytochemical analysis and then assess its antioxidant and antibacterial activities. Metabolite extraction will be carried out with an ethanol solvent, and antioxidant activity will be determined using the α, α-diphenyl-β-picrylhydrazyl (DPPH) assays. Antibacterial experiments were conducted against two types of bacteria: Escherichia coli and Staphylococcus aureus. From the results of the phytochemical investigation, the predominant compound group is alkaloid. The ethanol extract metabolites have antioxidant activity and belong to the strong antioxidant group with an IC₅₀ value of 77.96 ppm. Antibacterial activity was weak at concentrations as high as 20%. Based on these findings, further assays can be conducted, such as cytotoxic activity against various cancer cells.

Pengaruh Penyimpanan Suhu Refrigerasi terhadap Karakteristik Fisik Organoleptik, Nilai pH, Cemaran Coliform dan Salmonella spp., Yoghurt Susu Kambing dengan Penambahan Tepung Bekatul Beras Merah

Ajeng Erika Prihastuti Haskito — 2024-07-01

Yogurt is a fermented milk product made with the help of Lactic Acid Bacteria (LAB). Goat milk yogurt with the addition of red rice bran flour contains high antioxidants, making it very beneficial for health. This study aims to evaluate the effect of storage duration on the organoleptic physical characteristics, pH value, and contamination by Coliform and Salmonella spp. in goat milk yogurt with the addition of red rice bran flour. The study used 20 samples divided into five treatment groups based on storage duration (0, 1, 2, 3, and 4 weeks) at 4 ℃, with four repetitions. Each week, organoleptic physical characteristics were tested hedonically and hedonic quality assessed by 20 semi-trained panellists, pH measured with a digital pH meter, Coliform contamination tested using the Most Probable Number (MPN) method, and Salmonella spp. contamination tested using SSA media followed by TSIA media. Data analysis for organoleptic physical characteristics was conducted using the Kruskal-Wallis test followed by the Mann-Whitney test. Data analysis for pH and Coliform contamination was conducted using one-way ANOVA followed by the Tukey test. Data analysis for Salmonella spp. contamination was done descriptively. The results showed that the storage duration significantly affected the physical characteristics of appearance, taste, and aroma (p<0.05) but did not significantly affect colour (p>0.05). There were very significant differences in pH values and Coliform contamination (p<0.01), whereas Salmonella spp. contamination was not affected by the storage duration. The conclusion of this study is that goat milk yogurt with the addition of red rice bran flour should be consumed within three weeks based on organoleptic physical characteristics, pH value, and levels of Coliform and Salmonella spp. contamination.

Strategi Pengembangan Usaha Ternak Kerbau di Kabupaten Sumba Timur

Bernardus A. Bulu — 2024-07-01

Buffalo livestock in the culture of the people of East Sumba have a large role, both as working livestock, social and cultural livestock such as traditional mourning events, weddings and traditional houses. However, the government's role in developing the buffalo farming business is very low and the lack of development by breeders causes a decline in buffalo livestock productivity, resulting in a decline in population and an impact on fluctuating buffalo livestock prices. This research aims to: (1) develop a strategy for developing the buffalo population in East Sumba Regency. This research uses SWOT analysis to formulate a strategy for developing a buffalo livestock business. This research uses a survey method and purposive sampling with the minimum criteria of having three buffalo, having sold livestock in the last two years and being a fairly influential figure in the village. Primary and secondary data are used to answer the objectives of this research. The results of the SWOT analysis show that the buffalo farming business is in quadrant I with an X value = 0.16 and a Y value = 0.52. The strategy used is an aggressive strategy. The strategy for developing buffalo livestock is optimizing pastures by using feed processing technology so that feed is always available and increasing the production and productivity of buffalo livestock to maintain the availability and demand for buffalo livestock.

Gambaran Hematologi Darah Pasca Vaksinasi Porcine Reproductive and Respiratory Syndrome (PRRS) pada Ternak Babi di Kabupaten Kupang

Marsyella Gloria Sole — 2024-07-21

The potential for expanding pig farming, particularly with native pigs, in East Nusa Tenggara (NTT) Province is significant due to the predominant non-Muslim population in NTT who utilise pigs in traditional and religious ceremonies. However, the high number of pigs in Kupang Regency and conventional farming practices can elevate the likelihood of infections like Porcine Reproductive and Respiratory Syndrome, known as PRRS. Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a significant disease in the pig farming sector. The porcine reproductive and respiratory syndrome virus is highly contagious within pig herds. It can spread through direct contact or contaminated aerosols. Vaccines have been used to limit the disease’s transmission, despite often being considered ineffective. This study analyses the variations in blood haematological parameters before and after PRRS immunisation, including RBC, HGB, PCV, MCV, MCH, MCHC, and WBC. Fifteen pigs were included in the sample. Sampling was conducted two times from the same pigs: before PRRS vaccination and one month post-immunization. SPSS was used for the data analysis. A statistical study using SPSS showed no significant difference in blood hematology following the PRRS vaccination.