JURNAL KAJIAN VETERINER

Motilitas Spermatozoa Babi pada Pengencer Kuning Telur Ayam Kampung dengan Penambahan Glukosa yang Disimpan pada Suhu Preservasi

Wilhelmina Afra Bala, Nancy D. F. K. Foeh, Cynthia Dewi Gaina — Wed, 25 Jun 2025 01:42:21 +0000

Liquid semen is one way of preserving semen which is carried out for short storage purposes at a temperature of preservation. with the help of a diluent. boar semen is different from other ruminant semen, so it needs special handling. Storage of pig semen at cold temperatures can reduce the motility and viability of spermatozoa. The aim of this study was to maintain the quality of pig semen by adding coconut water and native chicken egg yolk diluent with the addition of glucose and stored at a temperature preservation. Boar semen was collected from sexually mature adult boar aged 2 years, the collection was carried out by the massage method. Semen that has been accommodated, is evaluated macroscopically and microscopically to see the quality of the cement, before dilution is carried out. This study was designed with a completely randomized design and factorial pattern with 2 control groups, 4 treatment groups, and 4 replications. The control group consisted of fresh semen without diluent. K0 The treatment groups were P1 (80CW + 20% NCEY + 20 L glucose), P2 (80% CW + 20% NCEY + 40 L glucose), P3 (80% CW + 20% NCEY + 60 L glucose) and P4 (80 % CW + 20% NCEY + 90 L glucose), which was preserved by the water jacket method. Spermatozoa motility and viability were evaluated every 2 hours until the motility value reached 40%. The results showed that P3 was the best combination to preserve cement (32 hours).

Analisis Fisikokimia dan Aktivitas Antibakteri Madu Hutan Timor terhadap Methicillin-Resistant Staphylococcus aureus

Fajar Okta Undari, Hadri Latif, Mirnawati Bachrum Sudarwanto — Wed, 25 Jun 2025 01:42:54 +0000

The emergence of antibiotic-resistant bacteria has become a global concern. One of the bacteria that is resistant to several types of antibiotics is Methicillin-Resistant Staphylococcus aureus (MRSA). Honey is known for its nutritional and antibacterial properties. The antibacterial activity of honey is due to several factors such as its physicochemical properties, H₂O₂, and phytochemicals compounds. Honey could be a promising alternative to overcome the problem of antibiotic resistance. The aim of this research was to determine the physicochemical, H₂O₂, Pinocembrin, Kaempferol and antimicrobial activity of Timor Forest honey. A total of 7 Timor Forest honey was analyzed to determine water content, water activity, pH, H₂O₂, Pinocembrin, Kaempferol and antibacterial activity. Moisture content, water activity, and pH were measured using a refractometer, aw-meter, and pH meter, respectively. Hydrogen peroxide content was measured using peroxide strip test. Pinocembrin and Kaempferol contents were measured using HPLC-DAD. The analysis showed that the water content, water activity and pH of Timor Forest honey were 17.52±3.31%, 0.6±0.01, 4.18±0.32, respectively. The content of H₂O₂, Pinocembrin, and Kaempferol were 109.1±47.1 µmol/L, 2.2±1.9 mg/100 g, 1.7±0.7 mg/100 g, respectively. The antibacterial activity of Timor Forest honey against MRSA was tested using Kirby-Bauer disc diffusion method. Vancomycin was used as positive control. The inhibition zone of Timor Forest honey and vancomycin was not significantly different with an average of 13.93±0.23 mm for Timor Forest honey and 14.33±0.05 mm for vancomycin. Timor forest honey has good physicochemical properties with a relatively high content of Pinocembrin and Kaempferol and has antibacterial activity against MRSA.

Correlation and Regression Analysis of Body Weight, Scrotal Circumference, and Semen Volume in Thin-tailed Rams at the Teaching Farm University of Lampung

Wed, 25 Jun 2025 01:43:54 +0000

Kualitas Domba Ekor Tipis jantan dapat diketahui dari performa produktivitas berupa bobot badan dan kualitas reproduksi berupa lingkar skrotum dan volume semen yang merupakan indikator kualitas reproduksi untuk keberhasilan fertilisasi. Penelitian ini mengnalisis korelasi dan regresi antara bobot badan (BB), lingkar skrotum (LS), dan volume semen (VS) pada sepuluh ekor Domba Ekor Tipis jantan (Ovis aries) berumur 8–18 bulan di Teaching Farm, Jurusan Peternakan, Fakultas Pertanian, Universitas Lampung. Metode purposive sampling digunakan untuk memilih hewan percobaan. Pengukuran BB (kg) dilakukan sebelum pemberian pakan pagi, LS (cm) diperoleh dari rerata dua kali pengukuran, dan koleksi VS (ml) menggunakan vagina buatan. Setiap parameter diukur sebanyak tiga kali dengan selang waktu empat hari, menghasilkan total 30 data (n=30). Data dianalisis menggunakan perangkat lunak R dengan uji korelasi Pearson untuk menentukan kekuatan dan arah hubungan antarvariabel, serta regresi linear untuk memodelkan hubungan prediktif antara: (1) BB dan LS, (2) BB dan VS, dan (3) LS dan VS. Hasil penelitian menyajikan korelasi positif kuat antara BB dan LS (r = 0,623; Y = 17,689 + 0,242X), korelasi positif sedang antara BB dan VS (r = 0,505; Y = -0,153 + 0,056X), serta korelasi positif sedang antara LS dan VS (r = 0,406; Y = 21,010 + 1,428X). Kesimpulan penelitian ini menunjukkan bahwa bobot badan dan lingkar skrotum dapat digunakan sebagai indikator fenotipik untuk memprediksi volume semen, sehingga dapat menjadi kriteria praktis dalam seleksi Domba Ekor Tipis jantan unggul.

Keanekaragaman Ektoparasit di Peternakan Sapi Potong Astomulyo Lampung Tengah dan Peternakan Sapi Perah Kawasan Usaha Peternakan Rakyat (KUNAK) Bogor

Nadim Shodiq, Supriyono Supriyono, Herwin Pisestyani — Wed, 25 Jun 2025 01:44:39 +0000

Ectoparasites in livestock areas can cause losses to farmers, such as decreased body weight and milk production due to anemia, disease transmission, and animal discomfort. This study aimed to identify the diversity, abundance, and potential risk of diseases that can be transmitted by ectoparasites as disease vectors in cattle. The study was conducted in two selected locations: Astomulyo beef cattle farm in Central Lampung and KUNAK dairy farm in Bogor. All ectoparasites around the pens were collected using sweep nets, aspirators, light traps and forceps. Identification results showed a diverse range of ectoparasites, including mosquitoes (Culicidae), flies (Muscidae), ticks (Haemaphysalis), Culicoides (Ceratopogonidae) and fleas (Haematopinidae). The environment, such as pens conditions, storage facilities, manure management, feed waste, and sanitation, influenced the diversity of ectoparasite species found in the study sites. The identified ectoparasite species have the potential to be vectors of various livestock diseases that lead to decreased production. This study is expected to provide information related to effective disease prevention and control strategies.

Molecular Identification of African Swine Fever Virus from an Outbreak in Kupang Region

Wed, 25 Jun 2025 01:45:43 +0000

African Swine Fever (ASF) adalah infeksi virus penting pada babi yang belakangan ini muncul kembali sebagai penyakit lintas batas yang menyebar dengan luas dan cepat. Konfirmasi pertama ASF tercatat di Indonesia pada akhir tahun 2019 dan kemudian meluas ke beberapa daerah termasuk Nusa Tenggara Timur (NTT). Informasi tentang virus ASF di NTT terbatas hingga saat ini. Studi ini bertujuan untuk mengidentifikasi keberadaan virus ASF pada kasus kematian massal babi di Kupang, Pulau Timor. Sampel ginjal dan limpa diambil dari delapan ekor babi yang mati mendadak di daerah Kupang. Teknik PCR konvensional dilakukan untuk mengidentifikasi daerah yang sangat terkonservasi dari genom virus ASF VP72. Hasil PCR menunjukkan bahwa semua sampel menampilkan panjang pita yang sesuai dengan pita target dari primer yang digunakan. Hasil ini menunjukkan bahwa virus ASF masih beredar di wilayah Kupang dan tetap menjadi ancaman bagi peternakan babi di daerah tersebut. Oleh karena itu, usaha untuk meningkatkan kesadaran publik terutama di kalangan produsen babi mengenai pentingnya penerapan biosekuriti harus dilakukan secara konsisten.

Escherichia coli Sensitivity Test on Meat and Liver of Broiler Chicken in Kupang City

Novianti N. Toelle, Julita D. M. Yasa, Jois M. Jacob, Devi Y. J. A. Moenek — Wed, 25 Jun 2025 01:46:23 +0000

Penelitian ini bertujuan untuk menguji sensitivitas Escherichia coli yang diisolasi dari daging dan hati ayam broiler yang dijual di Kota Kupang terhadap beberapa antibiotik. Sebanyak 30 isolat E. coli diidentifikasi dan diuji sensitivitasnya menggunakan media Muller-Hinton Agar (MHA) dengan empat jenis antibiotik, yaitu tetrasiklin (30μg), penisilin (10μg), gentamisin (10μg), dan oxasilin (10μg). Kekeruhan bakteri disesuaikan dengan standar McFarland No. 5 (1,5 x 10⁸ CFU/ml). Cakram antibiotik diletakkan pada permukaan media MHA yang telah diinokulasi dengan bakteri dan kemudian diinkubasi pada suhu 37°C selama 24 jam. Zona hambat yang terbentuk diukur dengan jangka sorong dan hasilnya diinterpretasikan sesuai dengan pedoman CLSI (2020). Hasil uji menunjukkan bahwa E. coli paling sensitif terhadap gentamisin (90%), diikuti tetrasiklin (60%), penisilin (30%), dan oxasilin (20%). Uji intermediat menunjukkan hasil pada tetrasiklin dan penisilin masing-masing sebesar 10%. Resistensi tertinggi ditemukan pada oxasilin (80%) dan penisilin (60%), sedangkan gentamisin menunjukkan resistensi terendah (10%). Data diuji statistik menggunakan ANOVA yang dilanjutkan dengan uji berganda Duncan untuk menentukan perbedaan antar kelompok. Hasil penelitian ini menunjukkan pentingnya pemantauan penggunaan antibiotik di peternakan ayam untuk mencegah perkembangan resistensi antibiotik yang lebih luas.