Validation of Spectrophotometric Method for Analysis of Anionic Surfactant Dodecyl Benzene Sulphonate (DBS) in Catfish (Clarias batrachus) Using Malachite Green
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Hermawan Purba(1*)
Seklah Tinggi Ilmu Kesehatan (STIKes) Senior
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Adhitasari Suratman(2)
Gadjah Mada University
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Eko Sugiharto(3)
Gadjah Mada University
(*) Corresponding Author
Abstract
Validation method of DBS anionic surfactant analysis on Clarias batrachus has been conducted. The method of analysis was divided into twophase, namely the extraction with solid-liquid extraction
using Soxhlet and analysis DBS. The extraction was performed using n-hexane and methanol for 9 and 6 hours, respectively. The analysis was performed using Spectrophotometer UV-Vis based on the complex formation of surfactant-malachite green (DBS-MG). These methods are applied to determine DBS accumulation of Clarias batrachus with DBS concentration exposure and DBS concentration of Clarias batrachus in markets. The result showed that the parameters of validation methods has high acceptability as linearity (R2 = 0.99), limit of detection (LOD) and limit of quantification (LOQ) (0.029 mg/L and 0.089 mg/L), sensitivity (ε = 38.15 × 104 L mol-1 cm-1), precision (RSD = 0.10-1.83 %) and accuracy (recovery = 80-92 %). The result of analysis of DBS in Clarias
batrachus with 2.5; 5; 10; 15 mg/L of DBS concentration exposure obtained 5.5; 6.8; 7.9; 8.7 mg/L respectively and Clarias batrachus from markets in a range 2.0-4.2 mg/L. The result showed that the analysis of DBS anionic surfactants using MG can be applied for Clarias batrachus.
Keywords: validation method, extraction, clarias batrachus, dodecyl benzene sulfonate, malachite green
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