Validation of Spectrophotometric Method for Analysis of Anionic Surfactant Dodecyl Benzene Sulphonate (DBS) in Catfish (Clarias batrachus) Using Malachite Green

  • Hermawan Purba(1*)
    Seklah Tinggi Ilmu Kesehatan (STIKes) Senior
  • Adhitasari Suratman(2)
    Gadjah Mada University
  • Eko Sugiharto(3)
    Gadjah Mada University
  • (*) Corresponding Author
Keywords: validation method, extraction, clarias batrachus, dodecyl benzene sulfonate, malachite green


Validation method of DBS anionic surfactant analysis on Clarias batrachus has been conducted. The method of analysis was divided into twophase, namely the extraction with solid-liquid extraction
using Soxhlet and analysis DBS. The extraction was performed using n-hexane and methanol for 9 and 6 hours, respectively. The analysis was performed using Spectrophotometer UV-Vis based on the complex formation of surfactant-malachite green (DBS-MG). These methods are applied to determine DBS accumulation of Clarias batrachus with DBS concentration exposure and DBS concentration of Clarias batrachus in markets. The result showed that the parameters of validation methods has high acceptability as linearity (R2 = 0.99), limit of detection (LOD) and limit of quantification (LOQ) (0.029 mg/L and 0.089 mg/L), sensitivity (ε = 38.15 × 104 L mol-1 cm-1), precision (RSD = 0.10-1.83 %) and accuracy (recovery = 80-92 %). The result of analysis of DBS in Clarias 
batrachus with 2.5; 5; 10; 15 mg/L of DBS concentration exposure obtained 5.5; 6.8; 7.9; 8.7 mg/L respectively and Clarias batrachus from markets in a range 2.0-4.2 mg/L. The result showed that the analysis of DBS anionic surfactants using MG can be applied for Clarias batrachus.

Keywords: validation method, extraction, clarias batrachus, dodecyl benzene sulfonate, malachite green


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Gonzalez-Roca E, Garcia-Albéniz X, Rodriguez-Mulero S, Gomis RR, Kornacker K, Auer H. Accurate Expression Profiling of Very Small Cell Populations. PLoS ONE 2010; 5(12): e14418. DOI:

Haunted HG. Types and Typical Ingredients of Detergents, Marcel Dekker, New York. 2004. Huang XL and Zhan JZ. Surfactant-sensitized malachite green method for trace determination of orthophosphate in aqueous solution. Analytica Chimica Acta 2006; 580: 55–67. DOI:10.1016/j.aca.2006.07.046

Jadhav D, Rekha, B N, Gogate PR, Rathod VK. Extraction of Vanillin from Vanilla Pods: a Comparison Study of Conventional Soxhlet and Ultrasound Assisted Extraction, J. Food Engineer; 2009; 93: 21-426. DOI:

Kargosha K, Ahmadi SH, Mansourian M, and Azad J. Simultaneous Determination of One Nonionic and Two Anionic Surfactants Using Forier Transform Infrared Spectrometry and Multivariate Analysis, Talanta; 2007; 75: 589-593. DOI:

Leon V M, Lopez C, Martin P A L, Prats D, Varo P, and Mazo EG. Removal of linear Alkylbenzene Sulfonate and Their Degradation Intermediates at low Temperatures During Activated Sludge Treatment. Chemosphere; 2006; 64: 11571166. DOI:

Maenpaa K and Kukkonen JVK. Bioaccumulation and Toxicity of 4-Nonylphenol (4-NP) and 4-(2-Dodecyl)-Benzene Sulfonate (LAS) in LumbriculusVariegatus (Oligochaeta) and ChironomusRiparius (Insecta), Aquat. Toxico;. 2006; 77: 329338. DOI:

Martin P A L, Parra AG, Mazo E G. Simultaneous Extraction and Determination of Anionic Surfactants in Waters and Sediments, J. Chromatogr A; 2006; 1114: 205-210. DOI:

Munoz D A, Saez M, Martin P A L, Parra AG, Mazo EG. New Extraction Method for the Analysis of Linear Alkylbenzene Sulfonates in Marine Organisms Pressurized Liquid Extraction versus Soxhlet Extraction, J. Chromatogr. A;, 2004; 1052: 33-38. DOI:

Olkowska E, Polkowska Z, and Namiesnik J. Review: Analytical Procedures for the Determination of Surfactants In Environmental Samples, Talanta; 2012; 88, 1-13. DOI:

Otzen, D. Protein-Surfactant Interaction: A Tale of Many States, Biochim. Biophys.Acta; 2011; 1814: 562-591.

Pérez ELA, Agüera A, Hernando MD, Malato S, and Fernández-Alba AR. Photodegradation of malachite green under natural sunlight irradiation: Kinetic and toxicity of the transformation products. Chemosphere. 2008; 70, 11: 2068-2075. DOI:

Saez M, Leon V M, Parra A G, and Mazo EG. Extraction and Isolation of Linear Alkylbenzene Sulfonates and Their Intermediate Metabolites from Various Marine Organisms, J. Chromatogr.,
2000; 889: 99-104. DOI:

Santoso L. Kajian Toksiksitas Dan Bioakumulasi Surfaktan Detergen Linear Alkilbenzen Sulfonate (LAS) Pada Udang Galah Macrobrachiumrosenbergi, Tesis, IPB, Bogor; 2010.

Savin S B. Fundamental of Analytical Chemistry, CRC Crit. Rev. Chem; 1979; 8: 55.

Schneider A, Hommel G, and Blettner M. Linear Regression Analysis. Dtsch Arztebl Int. 2010; 107(44): 776–782. DOI: 10.3238/arztebl.2010.0776.

Versteeg D J and Rawling J M. Bioconcentration and Toxicity of Dodecylbenzene Sulfonate (C12 LAS) to Aquatic Organisms Exposed in Experimental Streams, Arch. Environ.Contam.Toxicol; 2003; 44: 237-246. DOI:

Zoller U. Handbook of Detergent Part C: Analysis, Marcel Dekker, New York; 2005.

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Purba, H., Suratman, A., & Sugiharto, E. (2018). Validation of Spectrophotometric Method for Analysis of Anionic Surfactant Dodecyl Benzene Sulphonate (DBS) in Catfish (Clarias batrachus) Using Malachite Green. Journal of Applied Chemical Sciences, 5(2), 483-487. Retrieved from

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