Jurnal Veteriner Nusantara

Aeromonas hydrophila Infection in Bekko Koi Linked to Biosecurity Failure During Fish Introduction

2026-09-02T04:33:18+00:00

Aeromonas hydrophila is an opportunistic pathogen commonly associated with infections in koi fish, particularly under conditions of stress and suboptimal husbandry. This study aimed to evaluate the efficacy of a combined antibiotic and supportive therapeutic regimen in the management of Aeromonas hydrophila infection in Bekko koi. The outbreak was precipitated by the introduction of new fish without adequate quarantine and acclimatization procedures, as well as the influx of transport water contaminated with organic matter into the culture system. Treatment consisted of intramuscular administration of Oxytetracycline (80 mg/kg body weight), Dexamethasone (0.2 mg/kg body weight), and Biodin Romindo (0.1 mL/kg body weight) at two-day intervals over a 7-day period. Clinical evaluation demonstrated progressive improvement, as evidenced by a reduction in ulcerative lesions and hemorrhage, alongside restoration of scale integrity, mucus production, activity, and feeding behavior. Despite the completion of therapy, mild hemorrhage persisted in the caudal region, likely attributable to limited vascularization in the affected tissue. Overall, the therapeutic protocol proved effective in accelerating the recovery process in Bekko koi affected by Aeromonas hydrophila infection.

Optimasi Metode Ekstraksi DNA Cacing Dewasa Haemonchus contortus untuk Uji Molekuler

2026-09-02T14:22:53+00:00

Haemonchosis is a gastrointestinal helminth infection caused by Haemonchus contortus. PCR-based molecular testing has high sensitivity and specificity, enabling more accurate identification of H. contortus than other diagnostic methods. However, adult H. contortus worms have a complex and strong body structure, so it is necessary to optimize various effective extraction methods to remove DNA to obtain optimal PCR results. The aim of this study was to obtain the most optimal extraction method for H. contortus adult worms for molecular identification. The adult worm sample extraction method consisted of 6 method, namely a group of male adult worms cut along 1 mm, female adult worms cut along 1 mm, male adult worms crushed, female adult worms crushed, male adult worms heated, and female adult worms heated. The results of PCR products read by electrophoresis showed the brightest DNA band in the method of adult worms cut along 1 mm with a DNA band length of 260 bp. This method is also the most efficient to do because it uses the least time and energy compared to other method.

MULTIDRUG RESISTANCE (MDR) Escherichia coli BERSUMBER FESES SEGAR KUDA CIDOMO DI PASAR AMPENAN KOTA MATARAM

2026-09-23T02:46:07+00:00

Escherichia coli is a normal commensal bacterium in the gastrointestinal tract of animals; however, some strains may become pathogenic and develop antimicrobial resistance. This study aimed to identify E. coli from the feces of Cidomo horses at Kebon Roek Market, Ampenan, and to determine their antibiotic resistance profile, including the presence of multidrug-resistant (MDR) isolates. A total of 36 fresh fecal samples were collected purposively and cultured on Eosin Methylene Blue Agar (EMBA), followed by Gram staining and biochemical tests for confirmation. Antimicrobial susceptibility was assessed using the disk diffusion method against streptomycin, aztreonam, tetracycline, ciprofloxacin, and trimethoprim–sulfamethoxazole, and interpreted according to CLSI (2020) guidelines. MDR isolates were further tested using the Double Disk Synergy Test (DDST) to detect Extended Spectrum Beta-Lactamase (ESBL) production. The results showed that 7 of 36 samples (19.44%) were confirmed as E. coli. High resistance levels were observed against streptomycin and aztreonam, whereas tetracycline and ciprofloxacin exhibited the highest effectiveness. Three isolates (42.85%) were classified as MDR, displaying resistance to ≥3 antibiotic classes. All MDR isolates tested negative for ESBL production using DDST. These findings indicate that Cidomo horses may act as reservoirs of antibiotic-resistant E. coli in market environments. Strengthening hygiene management and promoting prudent antimicrobial use in animals are essential to reduce the risk of antimicrobial resistance transmission within the One Health framework.

PEMBERIAN TEPUNG DAUN SUNGKAI (Peronema canescens Jack) SEBAGAI FEED ADDITIVE DALAM RANSUM TERHADAP ORGAN PENCERNAAN BROILER

2026-09-23T07:17:16+00:00

This study aims to determine the effect of providing sungkai leaf flour (Peronema canescens Jack) as a feed additive in rations on the digestive organs of broilers. The study was conducted at Jalan Amula Rahayu RT.07, Marga Rahayu Village, South Lubuklinggau II District, Lubuklinggau City. The broiler rearing was carried out for 35 days, with an altitude of 142 meters above sea level. This study used a Completely Randomized Design (CRD) consisting of 6 treatments with 4 replications, resulting in 24 experimental units, each containing 4 DOCs. The treatments in this study were P0: 0% (Sungkai leaves), P1: 0.25% (Sungkai leaf flour), P2: 0.5% (Sungkai leaf flour), P3: 0.75% (Sungkai leaf flour), P4: 1% (Sungkai leaf flour), P5: 1.25% (Sungkai leaf flour). If the treatment showed a significant to very significant effect, a further DMRT test was carried out. The results of the study showed that the treatment of providing sungkai leaf flour (Peronema canescens Jack) as a feed additive in the ration on the digestive organs of broilers had no significant effect (P>0.05) on all parameters. The conclusion of this study is that the provision of sungkai leaf flour (Peronema canescens Jeck) in the ration has not been able to provide a better effect than the control on the digestive organs of broilers. The P4 treatment at the 1% sungkai leaf level tends to provide the best results with a small intestine weight value (27.02 g). Keywords: Broiler, Feed Additive, Digestive Organs, Sungkai Leaf Flour.

Uji Resistansi Pseudomonas aeruginosa terhadap Antibiotika pada Ikan Mas (Cyprinus carpio) di Kabupaten Kupang

2026-09-24T07:54:36+00:00

Common carp (Cyprinus carpio) is an important freshwater aquaculture commodity with high economic value; however, intensive farming practices increase the risk of bacterial diseases that can cause significant economic losses. One of the pathogenic bacteria frequently infecting common carp is Pseudomonas aeruginosa, while excessive and uncontrolled antibiotic use may contribute to bacterial resistance. This study aimed to analyze the resistance level of P. aeruginosa isolated from common carp against several antibiotics in Kupang Regency. Samples consisted of common carp showing clinical signs of bacterial infection, including reddish spots, scale detachment, and wounds on the body surface. Bacterial isolation was performed using Pseudomonas Agar Base (PAB), followed by identification through colony morphology, Gram staining, and biochemical tests comprising Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), and Simon’s Citrate Agar (SCA). Antibiotic susceptibility testing was conducted using the Kirby–Bauer disk diffusion method with gentamicin, ciprofloxacin, and chloramphenicol, and inhibition zones were interpreted according to Clinical and Laboratory Standards Institute (CLSI) standards. The results confirmed the presence of P. aeruginosa in infected common carp and revealed varying levels of susceptibility to the tested antibiotics. These findings indicate the occurrence of antibiotic resistance and emphasize the importance of prudent antibiotic selection and use in common carp health management.