https://ejurnal.undana.ac.id/index.php/jvn <p>Jurnal Veteriner Nusantara menerbitkan manuskrip yang relevan dalam bidang (tetapi tidak terbatas pada) ilmu veteriner, peternakan, perikanan, biologi hewan, One Health, zoonosis, dan kesejahteraan hewan. Jurnal Veteriner Nusantara merupakan salah satu jurnal di Universitas Nusa Cendana (UNDANA).</p> <p>e-ISSN:&nbsp;2540-7643</p> Program Studi Kedokteran Hewan, Universitas Nusa Cendana en-US Jurnal Veteriner Nusantara 2540-7643 https://ejurnal.undana.ac.id/index.php/jvn/article/view/28269 <p>Aeromonas hydrophila is an opportunistic pathogen commonly associated with infections in koi fish, particularly under conditions of stress and suboptimal husbandry. This study aimed to evaluate the efficacy of a combined antibiotic and supportive therapeutic regimen in the management of Aeromonas hydrophila infection in Bekko koi. The outbreak was precipitated by the introduction of new fish without adequate quarantine and acclimatization procedures, as well as the influx of transport water contaminated with organic matter into the culture system. Treatment consisted of intramuscular administration of Oxytetracycline (80 mg/kg body weight), Dexamethasone (0.2 mg/kg body weight), and Biodin Romindo (0.1 mL/kg body weight) at two-day intervals over a 7-day period. Clinical evaluation demonstrated progressive improvement, as evidenced by a reduction in ulcerative lesions and hemorrhage, alongside restoration of scale integrity, mucus production, activity, and feeding behavior. Despite the completion of therapy, mild hemorrhage persisted in the caudal region, likely attributable to limited vascularization in the affected tissue. Overall, the therapeutic protocol proved effective in accelerating the recovery process in Bekko koi affected by Aeromonas hydrophila infection.</p> Kadek Ferdy Agastia Dwi Pratama ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2026-09-02 2026-09-02 9 2 1 9 10.35508/jvn.v9i2.28269 https://ejurnal.undana.ac.id/index.php/jvn/article/view/26511 <p><em>Haemonchosis is a gastrointestinal helminth infection caused by Haemonchus contortus. PCR-based molecular testing has high sensitivity and specificity, enabling more accurate identification of H. contortus than other diagnostic methods. However, adult H. contortus worms have a complex and strong body structure, so it is necessary to optimize various effective extraction methods to remove DNA to obtain optimal PCR results. The aim of this study was to obtain the most optimal extraction method for H. contortus adult worms for molecular identification. The adult worm sample extraction method consisted of 6 method, namely a group of male adult worms cut along 1 mm, female adult worms cut along 1 mm, male adult worms crushed, female adult worms crushed, male adult worms heated, and female adult worms heated. The results of PCR products read by electrophoresis showed the brightest DNA band in the method of adult worms cut along 1 mm with a DNA band length of 260 bp. This method is also the most efficient to do because it uses the least time and energy compared to other method.</em></p> Ridi Arif Syafrina Atika Putri Nasution Muhammad Agil ##submission.copyrightStatement## http://creativecommons.org/licenses/by-sa/4.0 2026-09-02 2026-09-02 9 2 10 16 10.35508/jvn.v9i2.26511