JURNAL NUKLEUS PETERNAKAN https://ejurnal.undana.ac.id/index.php/nukleus <p style="text-align: justify;">Print ISSN:&nbsp;2355-9942<br>Electronic ISSN:&nbsp;2656-792X <br>DOI:&nbsp;https://doi.org/10.35508/nukleus</p> <p style="text-align: justify;">Jurnal Nukleus Peternakan&nbsp;(JNP) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JNP publishes more than 10 fully research articles, and invited reviews each year.&nbsp; Articles published in JNP encompass a broad range of research topics in animal production and fundamental aspects of genetics, reproduction, socioeconomic of livestock, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, endangered animals, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.</p> Universitas Nusa Cendana en-US JURNAL NUKLEUS PETERNAKAN 2355-9942 PENGENCER SEMEN YANG DIPERKAYA MINYAK IKAN MENJAGA KUALITAS SPERMATOZOA BABI (Sus scrofa domesticus) SELAMA PENYIMPANAN JANGKA PENDEK Fish Oil Enriched Semen Extender Protect Boar (Sus scrofa domesticus) Spermatozoa Quality During Short Term Storage https://ejurnal.undana.ac.id/index.php/nukleus/article/view/25850 <p>This study aimed to evaluate the effect of fish oil addition to semen extender on the quality of boar spermatozoa during 24-hour storage at 15°C. Fresh semen was collected from a healthy adult boar with initial motility ≥70% and diluted using a glukosa–sitrat–EDTA extender supplemented with 0 g (P0), 0.15 g (P1), 0.30 g (P2), and 0.45 g (P3) of fish oil per 1.000 mL. The experiment was arranged in a completely randomized design (CRD) with four treatments and four replications. Parameters observed included motility, viability, abnormality, and semen pH after 24-hour storage. Analysis of variance showed that fish oil addition had a highly significant effect (P&lt;0.01) on sperm viability, but no significant effects (P&gt;0.05) on motility, abnormality, or pH. The highest viability was found in P1 (93.25%) and the lowest in P3 (82.00%). Motility and abnormality improved at low doses but declined at higher concentrations. The pH values remained stable (6.7–7.2) across all treatments. In conclusion, adding a low dose of fish oil (0.15 g/1.000 mL) effectively maintained spermatozoa quality without altering extender chemistry, suggesting its potential as a natural additive for semen extender formulations in tropical artificial insemination programs.</p> Parsaoran Silalahi Pohan Panjaitan Imelda Tafonao ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-21 2026-06-21 13 1 1 7 10.35508/nukleus.v13i1.25850 THE EVALUATION OF FROZEN SEMEN PRODUCTION POTENTIAL IN ETAWAH GRADE AND ANGLO-NUBIAN BREEDS (Evaluasi Potensi Produksi Semen Beku pada Kambing Peranakan Etawah dan Anglo Nubian) https://ejurnal.undana.ac.id/index.php/nukleus/article/view/27812 <p>This study aimed to evaluate the potential of frozen semen production in Etawah Grade and Anglo-Nubian breeds based on fresh semen characteristics and to assess variations among individuals and breeds. The research was conducted at the Lembang Artificial Insemination Center, West Bandung, using primary data from five ejaculations per individual and secondary data from the year 2024. Fresh semen was evaluated for semen volume, sperm motility, and sperm concentration, reflecting the number of straws that can be produced per ejaculation. Frozen semen production potential was calculated based on semen volume, sperm motility, and sperm concentration. The results showed significant differences (P&lt;0.01) in production potential among individuals and between breeds. The highest production potential was observed in the buck named Andromeda (122.45 ± 2.07 straws), which was significantly higher than that of other individuals. Anglo-Nubian breed showed a higher production potential (105.76 ± 2.54 straws) compared to Etawah Grade (91.63 ± 1.92 straws). However, Etawah Grade bucks exhibited relatively better sperm motility. In conclusion, both individual and breed factors significantly influence frozen semen production potential. Anglo-Nubian bucks have higher production capacity, while Etawah Grade bucks may offer better sperm quality. These findings provide important insights for optimizing breeding strategies and semen production planning in artificial insemination programs.</p> Casmuti Casmuti Mokhamad Fakhrul Ulum Syahruddin Said Taufiq Purna Nugraha Asep Kurnia Raden Iis Arifiantini ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-21 2026-06-21 13 1 8 14 10.35508/nukleus.v13i1.27812 KARAKTERISTIK ORGANOLEPTIK DAN FISIKO-KIMIA PRODUK SUI WU’U DENGAN PENAMBAHAN TEPUNG BERAS MERAH (Organoleptic and Physico-Chemical Characteristics of Sui Wu’u Products wuth The Addition of Red Rice Flour) https://ejurnal.undana.ac.id/index.php/nukleus/article/view/24411 <p><em>Sui wu'u</em> is a pork product typical of the people of Flores (Ngada), East Nusa Tenggara, and has been passed down through generations since ancient times. This dish is made from pork preserved in a unique way using corn flour and salt, then stored in bamboo segments (<em>tuku</em>).This study aims to determine the effect of adding red rice flour on the organoleptic and physicochemical quality of <em>sui wu'u</em>. This study used a Completely Randomized Design (CRD) with 4 treatments and 4 replications. The treatments included P<sub>0</sub> (control without red rice flour), P<sub>1</sub> (10% red rice flour), P<sub>2</sub> (20% red rice flour), and P<sub>3</sub> (30% red rice flour) from corn flour. The results showed that the addition of red rice flour significantly affected the color of <em>sui wu'u</em> (P&lt;0.01), where the color changed from bright red to dark red at 30% treatment. Aroma, taste, and tenderness did not experience significant changes (P&gt;0.05). The treatment did not affect pH and water activity (P&gt;0.05), but significantly affected protein content, fat content, fat oxidation and anthocyanin (P&lt;0.01) with a decrease in protein and fat content and an increase in anthocyanin content at the highest treatment (P<sub>3</sub>). It was concluded that the addition of red rice flour up to 30% had a positive effect on several physico-chemical quality parameters (except protein content) of <em>sui wu'u</em> without significantly changing the organoleptic properties except color.</p> Rosalia Rosinta G. E. M Malelak Arnol E. Manu ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-21 2026-06-21 13 1 15 24 10.35508/nukleus.v13i1.24411 PENGARUH LEVEL OPTIMAL EKSTRA DAUN KELOR DALAM TIGA PENGENCER TERHADAP KEBERHASILAN INSEMINASI BUATAN TERNAK BABI SECARA IN VIVO (Effect of optimal Levels Of Moringa Leaf Extract Levels in Three ExtenderOn The Succes Of Artificial Insemination In Pigs... https://ejurnal.undana.ac.id/index.php/nukleus/article/view/27679 <p>Artificial insemination is a modern livestock breeding method that has been widely applied and proven effective in commercial farming systems. This study aimed to evaluate the success of artificial insemination using three types of semen extenders, namely Tris-egg yolk (T-EY), Citrate-egg yolk (C-EY), and Beltsville Thawing Solution-egg yolk (BTS-EY), supplemented with optimal levels of Moringa oleifera leaf extract (MLE), in order to determine the best extender. A total of 18 sows aged 1–2 years were inseminated using liquid semen. A descriptive method was applied to analyze non-return rate (NRR) and conception rate (CR), while a completely randomized design (CRD) was used for service per conception (S/C). The treatments consisted of P1: artificial insemination using T-EY + 2% MLE, P2: C-EY + 4% MLE, and P3: BTS-EY + 2% MLE. The observed variables were NRR, CR, and S/C. The results showed that the T-EY + 2% MLE extender achieved the highest CR and NRR, both reaching 100%, with an S/C value of 1.00. Meanwhile, the C-EY + 4% MLE and BTS-EY + 2% MLE treatments resulted in CR and NRR values of 83.33%, with an S/C value of 1.16. It can be concluded that the T-EY extender supplemented with 2% *Moringa oleifera* leaf extract was the best extender for liquid semen preservation of Landrace boars in artificial insemination programs.</p> Daniel Kurniawan Tulle Dani Tulle Wilmientje Marlene Nalley Thomas Mata Hine Henderiana Laura Louisa Belli Arnold Elyazar Manu ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-21 2026-06-21 13 1 25 32 10.35508/nukleus.v13i1.27679 CHEMICAL AND ORGANOLEPTIC QUALITY OF SMOKED SALTED EGGS WITH THE ADDITION OF HERBAL INGREDIENTS GINGER AND TURMERIC https://ejurnal.undana.ac.id/index.php/nukleus/article/view/22503 <p>The addition of herbal ingredients such as turmeric and ginger can increase the durability and shelf life of smoked salted eggs. The active compounds curcumin and gingerol are thought to play a role in increasing the quality of salted eggs. This study aims to analyze the effect of adding turmeric and ginger on the chemical and organoleptic properties of smoked salted eggs. The method used was a Completely Randomized Design (CRD) with four treatments: P<sub>0</sub> (control), P<sub>1</sub> (turmeric 7.5%), P<sub>2</sub> (ginger 7.5%), and P<sub>3</sub> (combination of turmeric and ginger 7.5%), each with five replications. The parameters observed included ash content, salt content, crude fiber, and organoleptic tests (color, aroma, texture, and preference). The results showed that the addition of turmeric and/or ginger had a significant effect on ash content, color, aroma and had a very significant effect on crude fiber content, but had no significant effect on salt content, texture and preference level. The mineral and crude fiber content in turmeric and ginger contributed to the increase in ash and crude fiber content. Meanwhile, the addition of turmeric and ginger in the salted egg mixture does not affect the salt content, so the salty taste remains balanced, neither excessive nor lacking. The best results were obtained in the combination treatment of 7.5% turmeric and 7.5% ginger (P<sub>3</sub>) with an ash content of 12.27%, a salt content of 4.00%, crude fiber of 4.41%, and an organoleptic score for color of 4.07, aroma of 3.60, and a preference level of 3.47. Thus, the use of a combination of turmeric and ginger can be an alternative in improving the quality and durability of smoked salted eggs without changing the balance of taste.</p> Salma Sania Andri Kusmayadi Putri Dian Wulansari ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-21 2026-06-21 13 1 33 42 10.35508/nukleus.v13i1.22503 EVALUATION OF THE SUSTAINABILITY SHEEP DEVELOPMENT IN GALANG, DELI SERDANG DISTRICT https://ejurnal.undana.ac.id/index.php/nukleus/article/view/25433 <p>This study aims to evaluate the sustainability of sheep farming in Galang, Deli Serdang from March-May 2023. This study used the Multi-Dimensional Scaling (MDS) method with the Rap-Sheep approach for sustainability assessment. The sensitive attributes influencing the sustainability index and sustainability status were analyzed using Leverage analysis, whereas the effects of uncertainty and potential errors were examined using Monte Carlo analysis. The data used in the study included primary data and secondary data. Respondents used amounted to 90 sheep farmers. This study uses 4 dimensions, namely the ecological dimension with 12 attributes, the economic dimension with 10 attributes, the social dimension with 9 attributes, and the technology dimension with 10 attributes. The results showed that the sheep farming in Galang, Deli Serdang currently has a sustainability index value in the ecological dimension of 56,71 (quite sustainable), economic dimension of 51,50 (quite sustainable), social dimension of 63,47 (quite sustainable) and technological dimension of 38,85 (less sustainable). Multi dimensionally, the index value of 52,63 is obtained, which means that the sheep farming in Galang, Deli Serdang is in a fairly sustainable status.</p> Galih Ari Wirawan Siregar Teguh Azira Perkasa Achmad Sadeli Rizka Ramadhani ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-21 2026-06-21 13 1 43 53 10.35508/nukleus.v13i1.25433 PENGARUH PEMBERIAN KONSENTRAT YANG MENGANDUNG RUMPUT LAUT EUCHEUMA COTTONII AFKIR TERFERMENTASI TERHADAP IMBANGAN NITROGEN SAPI BALI BETINA MUDA YANG DIBERIKAN PAKAN DASAR SILASE DAN FODDER JAGUNG (Effect of Feeding Concentrate Containing Rejected Seawee https://ejurnal.undana.ac.id/index.php/nukleus/article/view/20810 <p>This study aims to determine the effect of the feeding of concentrate containing fermented seaweed <em>Eucheuma cottonii</em> on the nitrogen balance of young female balinese cows fed silage basic feed or corn fodder. This study used 4 balinese heifers with an age range of two to three years with an initial body weight of 156.2 to 178.4 kg. The research method was an experimental method that used a Latin square design (RBSL) with 4 treatments and 4 periods as a replicate. The treatments were SKC0: natural grass silage 60% + concentrate containing <em>E. cottonii</em> without fermentation 40%, SKC0F: natural grass silage 60% + concentrate containing fermented <em>E. cottonii </em>40%, FJK0: corn fodder 60% + concentrate containing <em>E.cottonii</em> without fermmentation 40%, and FJK0F: corn fodder 60% + concentrate containing <em>E</em>. <em>cottonii</em> fermentation 40%. The parameters measured were N intake, N urine, N feces, and N balance. The results of this study showed that the feeding concentrate containing fermented <em>Eucheuma cottonii</em> with natural grass silage and corn fodder basic feed had a significant effect (P&lt;0.05) on nitrogen intake and fecal nitrogen. It was concluded that feeding concentrates containing fermented <em>E. cottonii</em> rejected increased nitrogen intake and fecal nitrogen. Meanwhile feeding concentrates containing fermented <em>E. cottonii</em> rejected do not affect the urinary nitrogen and nitrogen balance in heifers fed both natural grass silage and corn fodder basal diets</p> Goldensius Guru Gesi I Gusti Ngurah Jelantik Daud Amalo ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-21 2026-06-21 13 1 54 63 10.35508/nukleus.v13i1.20810 KUALITAS TELUR AYAM ARAB KONSUMSI DAN PERUBAHAN MUTUNYA SELAMA PENYIMPANAN PADA SUHU YANG BERBEDA (Quality Changes of Arabian Chicken Table Eggs During Storage at Different Temperatures) https://ejurnal.undana.ac.id/index.php/nukleus/article/view/24992 <p>The quality of Arabic Chicken table eggs (<em>Gallus turcicus</em>) and changes in quality during storage at room temperature (25–27 °C) and refrigerated temperature (4–7 °C) were evaluated. A total of 200 fresh eggs obtained from Arabic laying hens at peak production (30–40 weeks of age) were assigned to two storage temperature groups. Measurements were performed on days 0, 5, 10, 15, 20, 25, 30, 35, 40, and 45, using five eggs per group at each sampling time. External quality (egg weight and air cell height) and internal quality (yolk index, albumen index, and Haugh Unit) parameters were determined. Data were analyzed using two-way analysis of variance (ANOVA) to assess the effects of storage temperature, storage duration, and their interaction, followed by Tukey's post hoc test. Storage temperature, storage duration, and their interaction were found to have significant effects (P &lt; 0.05) on air cell height, yolk index, and Haugh Unit. A significant temperature–time interaction indicated that the rate of egg quality deterioration depended on both storage temperature and storage duration. Eggs stored at room temperature exhibited faster quality deterioration, which was detected as early as day 10 of storage, as evidenced by decreased yolk index and Haugh Unit values and increased air cell height. In contrast, eggs stored under refrigerated conditions showed greater stability, with internal quality maintained until days 40–45 of storage. Overall, refrigerated storage was more effective than room-temperature storage in maintaining the quality of Arabic chicken table eggs.</p> Herwin Pisestyani Erlangga Putra Wijaya Ekowati Handharyani Denny Widaya Lukman ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-21 2026-06-21 13 1 64 78 ANALISIS INDEKS ANIMAL WELFARE KUDA PADA PASAR KUDA TRADISIONAL (Analysis of the Horse Animal Welfare Index in Traditional Horse Market) https://ejurnal.undana.ac.id/index.php/nukleus/article/view/28376 <p>This study aims to analyze the animal welfare index for horses sold at the Horse Market in Jeneponto Regency. A mixed methods approach was used, combining quantitative and qualitative data. Quantitative data were obtained through observations of 100 horses using the Five Freedoms principle, assessed on a scale of 1-5, while qualitative data were collected through interviews with traders, buyers, and market managers. Data were analyzed descriptively by calculating the animal welfare index based on the average score of all observed indicators. The results showed that the horse animal welfare index was 2.16, which is considered adequate. The highest score was for freedom from pain/injury/disease (3.56), categorized as good, while the lowest score was for freedom from hunger and thirst (1.00), categorized as poor. Freedom from discomfort (1.73) and freedom to express natural behavior (2.00) were also considered low, while freedom from stress was considered adequate (2.50). The low implementation of animal welfare is influenced by limited market facilities, high livestock density, unhygienic environmental conditions, and market participants' poor understanding of animal welfare principles. This study shows that the implementation of animal welfare in horse trading in traditional horse markets is still not optimal, so that improvements in facilities, improved market management, and education related to animal welfare for all market players are needed to support a more sustainable and ethical livestock trading system.</p> Ayu Lestari Faris Makkawaru Syukri Jumriah Syam Handayani Indah Susanti Khaifah Asgaf Mursidin Mursidin Rusny Rusny ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 2026-06-22 2026-06-22 13 1 79 90 10.35508/nukleus.v13i1.28376