Analisis Genetik Gen Leptin Berdasarkan Sekuen DNA GenBank dan Asosiasinya dengan Reproduksi Ternak Babi
Genetic Analysis of the Leptin Gene Based on GenBank DNA Sequences and its Association with Pig Reproduction
-
Cynthia Dewi Gaina(1*)
Nusa Cendana University
-
Filphin Adolfin Amalo(2)
Laboratorium Anatomi, Fisiologi, Farmakologi, dan Biokimia Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana
-
Yustinus O. P. Wuhan(3)
Laboratorium Klinik, Reproduksi, Patologi dan Nutrisi, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana
(*) Corresponding Author
Keywords:
Leptin gene, Nucleotides, Pig, SNPs, Phylogenetic Tree
Abstract
A candidate gene suspected of affecting pigs' reproductive traits is known as the leptin (LEP) gene. Based on the information found in Leptin GenBank's DNA sequence database, this research aimed to determine single nucleotide polymorphisms (SNPs) and changes in nucleotides and phylogenetic trees in pigs. The data from NCBI GenBank was used to obtain three different DNA sequences of the Leptin gene in pigs. The DNA sequence data for leptin were aligned using BioEdit to establish the sites of SNPs and alterations in nucleotides. A phylogenetic tree was constructed using Mega X. DNA sequences were, and the pig LEP gene was sequenced resulted in 11 SNPs. These findings are based on the sequence regions. The phylogenetic analysis revealed that the sequence of the Leptin gene in pigs (Sus scrofa) clustered more closely with that of goats (Capra hircus) than it did with the sequence of the LEP gene among the cattle (Bos taurus and Bos indicus). The outcomes of this study can serve as a basis for more investigation into the connection between the LEP gene SNP and reproductive traits in pigs as one of potential genes for molecular selection.
Downloads
Download data is not yet available.
References
Aisah, I., Kurniadi, E., Carnia, E., & Ula, N. 2015. Representasi mutasi kode genetik standar berdasarkan basa nukleotida. Jurnal Matematika Integratif ISSN, 1412, 6184. https://doi.org/10.24198/jmi.v11.n1.9399.25-34
Balatsky, V. N., Oliinychenko, Y. K., Saienko, A. M., Buslyk, T. V., Bankovska, I. B., Peka, M. Y., & Doran, O. 2022. Associations of Polymorphisms in Leptin and Leptin Receptor Genes with Meat Quality in Pigs of the Ukrainian Large White Breed. Cytology and Genetics, 56(6):513-525. DOI: 10.15407/agrisp3.02.042
Laksa E. A., Hartatik, T., Panjono. Identifikasi Keragaman Gen Leptin serta Hubungannya dengan Berat Lahir Dan Ukuran Tubuh Sapi Potong. 2020. Skripsi. UGM.
Grădinaru, A. C., Mizeranschi, A. E., Mihali, C. V., Neamţ, R. I., Găină, V., Carabaş, M., & Ilie, D. E. 2020. Leptin gene polymorphism in Romanian cattle breeds and associations with milk production traits. Animal Biology & Animal Husbandry, 12(2): 76-85.
Hall, T., I. Biosciences & C. Carlsbad. 2011. BioEdit: an important software for molecular biology. GERF Bull Biosci 2:60–61.
Haruna, I. L., Hadebe, S. A., Oladosu, O. J., Mahmoud, G., Zhou, H., & Hickford, J. G. 2020. Identification of novel nucleotide sequence variations in an extended region of the bovine leptin gene (LEP) across a variety of cattle breeds from New Zealand and Nigeria. Archives Animal Breeding, 63(2): 241-248. DOI: 10.5194/aab-63-241-2020
Jannah, M., Sari, N. K., Mushlih, M., Hariri, M. R., Priyambodo, P., Pratiwi, R. H., & Awwanah, M. 2021. Metode Biologi Molekuler. Penerbit Widina.
Kuehn, L. A., Nonneman, D. J., Klindt, J. M., & Wise, T. H. 2009. Genetic relationships of body composition, serum leptin, and age at puberty in gilts. Journal of animal science, 87(2): 477–483. https://doi.org/10.2527/jas.2008-0936
Lents, C. A., Lindo, A. N., Hileman, S. M., & Nonneman, D. J. 2020. Physiological and genomic insight into neuroendocrine regulation of puberty in gilts. Domestic Animal Endocrinology, 73, 106446. DOI: 10.1016/j.domaniend.2020.106446
Liu, J., Cao, S., Liu, M., Chen, L., & Zhang, H. 2018. A high nutrient dense diet alters hypothalamic gene expressions to influence energy intake in pigs born with low birth weight. Scientific Reports, 8(1): 1-12. DOI: 10.1038/s41598-018-23926-x
Lv, D., Tan, T., Zhu, T., Wang, J., Zhang, S., Zhang, L., & Xing, Y. 2019. Leptin mediates the effects of melatonin on female reproduction in mammals. Journal of Pineal Research, 66(3), e12559. DOI: 10.1111/jpi.12559
Martins, K. R., Haas, C. S., Rovani, M. T., Moreira, F., Goetten, A. L., Ferst, J. G., ... & Lucia Jr, T. 2021. Regulation and function of leptin during ovarian follicular development in cows. Animal Reproduction Science, 227, 106689. https://doi.org/10.1016/j.anireprosci.2021.106689
Muro, B. B., Carnevale, R. F., Leal, D. F., Almond, G. W., Monteiro, M. S., Poor, A. P., & Garbossa, C. A. 2022. The importance of optimal body condition to maximise reproductive health and perinatal outcomes in pigs. Nutrition Research Reviews, 1-21. DOI:
10.1017/S0954422422000129
Moreira, F., Corcini, C. D., Mondadori, R. G., Gevehr-Fernandes, C., Mendes, F. F., Araujo, E. G., & Lucia Jr, T. 2013. Leptin and mitogen-activated protein kinase (MAPK) in oocytes of sows and gilts. Animal reproduction science, 139(1-4):89-94. DOI: 10.1016/j.anireprosci.2013.03.011
Perez-Montarelo, D., Fernandez, A., Barragan, C., Noguera, J. L., Folch, J. M., Rodríguez, M. C., & Fernandez, A. I. 2013. Transcriptional characterization of porcine leptin and leptin receptor genes. PLoS One, 8(6), e66398. https://doi.org/10.1371/journal.pone.0066398
Perez-Montarelo, D., Rodríguez, M. C., Fernandez, A., Benitez, R., Garcia, F., Silio, L., & Fernandez, A. I. 2015. Haplotypic diversity of porcine LEP and LEPR genes involved in growth and fatness regulation. Journal of applied genetics, 56: 525-533. DOI: 10.1007/s13353-015-0284-7
Priyadarshini, L., Yadav, A. K., Singh, H. S., Mishra, A., Jain, A. K., & Ahirwar, M. K. 2015. Role of leptin in physiology of animal reproduction-A review. Agricultural Reviews, 36(3): 235-240. DOI: 10.5958/0976-0741.2015.00027.6
Raza, K. 2012. Application of data mining in bioinformatics. arXiv Prepr. arXiv1205.1125.
Rybska, M., Knap, S., Jankowski, M., Jeseta, M., Bukowska, D., Antosik, P & Jaśkowski, J. M. 2018. Characteristic of factors influencing the proper course of folliculogenesis in mammals. Medical Journal of Cell Biology, 6(1): 33-38. https://doi.org/10.2478/acb-2018-0006
Scarano, D., & Rao, R. 2014. DNA markers for food products authentication. Diversity, 6(3): 579-596. https://doi.org/10.3390/d6030579
Sharma, M. V., Prabhakar Maurya, A., Pandey, R. R., Thakur, M. S., & Singh, S. N. 2023. Associations of exon 2 of leptin gene polymorphism with carcass traits in cross bred pigs. Journal of Animal Breeding and Genetics. DOI: 10.1111/j.1439-0388.2006.00611.x
Slawrys, G., & Gajewska, A. 2017. In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig. Polish journal of veterinary sciences, 20(1). DOI: 10.1515/pjvs-2017-0010
Smolinska, N., Kaminski, T., Siawrys, G., & Przala, J. 2013.
Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs. Animal, 7(1): 109-117. https://doi.org/10.1017/S1751731112001103
Solé, E., Ros-Freixedes, R., Tor, M., Reixach, J., Pena, R. N., & Estany, J. 2021. Antagonistic maternal and direct effects of the leptin receptor gene on body weight in pigs. PLoS One, 16(1), e0246198. https://doi.org/10.1371/journal.pone.0246198
Steri, M., Idda, M. L., Whalen, M. B., & Orrù, V. 2018. Genetic variants in mRNA untranslated regions. Wiley Interdisciplinary Reviews: RNA, 9(4), e1474. DOI: 10.1002/wrna.1474.
Takle, Z. J., & Legesse, T. G. 2017. The effect of leptin on the hypothalamic-pituitary gonadal axis and puberty. International Journal of Health Sciences and Research (7):332-344.
Wahab, F., Atika, B., & Shahab, M. 2013. Kisspeptin as a link between metabolism and reproduction: evidences from rodent and primate studies. Metabolism, 62(7):898-910. https://doi.org/10.1016/j.metabol.2013.01.015.
Balatsky, V. N., Oliinychenko, Y. K., Saienko, A. M., Buslyk, T. V., Bankovska, I. B., Peka, M. Y., & Doran, O. 2022. Associations of Polymorphisms in Leptin and Leptin Receptor Genes with Meat Quality in Pigs of the Ukrainian Large White Breed. Cytology and Genetics, 56(6):513-525. DOI: 10.15407/agrisp3.02.042
Laksa E. A., Hartatik, T., Panjono. Identifikasi Keragaman Gen Leptin serta Hubungannya dengan Berat Lahir Dan Ukuran Tubuh Sapi Potong. 2020. Skripsi. UGM.
Grădinaru, A. C., Mizeranschi, A. E., Mihali, C. V., Neamţ, R. I., Găină, V., Carabaş, M., & Ilie, D. E. 2020. Leptin gene polymorphism in Romanian cattle breeds and associations with milk production traits. Animal Biology & Animal Husbandry, 12(2): 76-85.
Hall, T., I. Biosciences & C. Carlsbad. 2011. BioEdit: an important software for molecular biology. GERF Bull Biosci 2:60–61.
Haruna, I. L., Hadebe, S. A., Oladosu, O. J., Mahmoud, G., Zhou, H., & Hickford, J. G. 2020. Identification of novel nucleotide sequence variations in an extended region of the bovine leptin gene (LEP) across a variety of cattle breeds from New Zealand and Nigeria. Archives Animal Breeding, 63(2): 241-248. DOI: 10.5194/aab-63-241-2020
Jannah, M., Sari, N. K., Mushlih, M., Hariri, M. R., Priyambodo, P., Pratiwi, R. H., & Awwanah, M. 2021. Metode Biologi Molekuler. Penerbit Widina.
Kuehn, L. A., Nonneman, D. J., Klindt, J. M., & Wise, T. H. 2009. Genetic relationships of body composition, serum leptin, and age at puberty in gilts. Journal of animal science, 87(2): 477–483. https://doi.org/10.2527/jas.2008-0936
Lents, C. A., Lindo, A. N., Hileman, S. M., & Nonneman, D. J. 2020. Physiological and genomic insight into neuroendocrine regulation of puberty in gilts. Domestic Animal Endocrinology, 73, 106446. DOI: 10.1016/j.domaniend.2020.106446
Liu, J., Cao, S., Liu, M., Chen, L., & Zhang, H. 2018. A high nutrient dense diet alters hypothalamic gene expressions to influence energy intake in pigs born with low birth weight. Scientific Reports, 8(1): 1-12. DOI: 10.1038/s41598-018-23926-x
Lv, D., Tan, T., Zhu, T., Wang, J., Zhang, S., Zhang, L., & Xing, Y. 2019. Leptin mediates the effects of melatonin on female reproduction in mammals. Journal of Pineal Research, 66(3), e12559. DOI: 10.1111/jpi.12559
Martins, K. R., Haas, C. S., Rovani, M. T., Moreira, F., Goetten, A. L., Ferst, J. G., ... & Lucia Jr, T. 2021. Regulation and function of leptin during ovarian follicular development in cows. Animal Reproduction Science, 227, 106689. https://doi.org/10.1016/j.anireprosci.2021.106689
Muro, B. B., Carnevale, R. F., Leal, D. F., Almond, G. W., Monteiro, M. S., Poor, A. P., & Garbossa, C. A. 2022. The importance of optimal body condition to maximise reproductive health and perinatal outcomes in pigs. Nutrition Research Reviews, 1-21. DOI:
10.1017/S0954422422000129
Moreira, F., Corcini, C. D., Mondadori, R. G., Gevehr-Fernandes, C., Mendes, F. F., Araujo, E. G., & Lucia Jr, T. 2013. Leptin and mitogen-activated protein kinase (MAPK) in oocytes of sows and gilts. Animal reproduction science, 139(1-4):89-94. DOI: 10.1016/j.anireprosci.2013.03.011
Perez-Montarelo, D., Fernandez, A., Barragan, C., Noguera, J. L., Folch, J. M., Rodríguez, M. C., & Fernandez, A. I. 2013. Transcriptional characterization of porcine leptin and leptin receptor genes. PLoS One, 8(6), e66398. https://doi.org/10.1371/journal.pone.0066398
Perez-Montarelo, D., Rodríguez, M. C., Fernandez, A., Benitez, R., Garcia, F., Silio, L., & Fernandez, A. I. 2015. Haplotypic diversity of porcine LEP and LEPR genes involved in growth and fatness regulation. Journal of applied genetics, 56: 525-533. DOI: 10.1007/s13353-015-0284-7
Priyadarshini, L., Yadav, A. K., Singh, H. S., Mishra, A., Jain, A. K., & Ahirwar, M. K. 2015. Role of leptin in physiology of animal reproduction-A review. Agricultural Reviews, 36(3): 235-240. DOI: 10.5958/0976-0741.2015.00027.6
Raza, K. 2012. Application of data mining in bioinformatics. arXiv Prepr. arXiv1205.1125.
Rybska, M., Knap, S., Jankowski, M., Jeseta, M., Bukowska, D., Antosik, P & Jaśkowski, J. M. 2018. Characteristic of factors influencing the proper course of folliculogenesis in mammals. Medical Journal of Cell Biology, 6(1): 33-38. https://doi.org/10.2478/acb-2018-0006
Scarano, D., & Rao, R. 2014. DNA markers for food products authentication. Diversity, 6(3): 579-596. https://doi.org/10.3390/d6030579
Sharma, M. V., Prabhakar Maurya, A., Pandey, R. R., Thakur, M. S., & Singh, S. N. 2023. Associations of exon 2 of leptin gene polymorphism with carcass traits in cross bred pigs. Journal of Animal Breeding and Genetics. DOI: 10.1111/j.1439-0388.2006.00611.x
Slawrys, G., & Gajewska, A. 2017. In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig. Polish journal of veterinary sciences, 20(1). DOI: 10.1515/pjvs-2017-0010
Smolinska, N., Kaminski, T., Siawrys, G., & Przala, J. 2013.
Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs. Animal, 7(1): 109-117. https://doi.org/10.1017/S1751731112001103
Solé, E., Ros-Freixedes, R., Tor, M., Reixach, J., Pena, R. N., & Estany, J. 2021. Antagonistic maternal and direct effects of the leptin receptor gene on body weight in pigs. PLoS One, 16(1), e0246198. https://doi.org/10.1371/journal.pone.0246198
Steri, M., Idda, M. L., Whalen, M. B., & Orrù, V. 2018. Genetic variants in mRNA untranslated regions. Wiley Interdisciplinary Reviews: RNA, 9(4), e1474. DOI: 10.1002/wrna.1474.
Takle, Z. J., & Legesse, T. G. 2017. The effect of leptin on the hypothalamic-pituitary gonadal axis and puberty. International Journal of Health Sciences and Research (7):332-344.
Wahab, F., Atika, B., & Shahab, M. 2013. Kisspeptin as a link between metabolism and reproduction: evidences from rodent and primate studies. Metabolism, 62(7):898-910. https://doi.org/10.1016/j.metabol.2013.01.015.
Published
2023-06-20
How to Cite
Gaina, C., Amalo, F., & Wuhan, Y. (2023). Analisis Genetik Gen Leptin Berdasarkan Sekuen DNA GenBank dan Asosiasinya dengan Reproduksi Ternak Babi. JURNAL KAJIAN VETERINER, 11(1), 93-102. https://doi.org/10.35508/jkv.v11i1.10162
Section
Articles
Copyright (c) 2023 JURNAL KAJIAN VETERINER
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
.png)
