Molecular Identification of African Swine Fever Virus from an Outbreak in Kupang Region
Identifikasi Molekuler Virus African Swine Fever yang Berasal dari Wabah di Daerah Kupang
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Maria Aega Gelolodo(1*)
Veterinary Diseases and Public Health Department, Faculty of Medicine and Veterinary Medicine, Nusa Cendana University
https://orcid.org/0000-0002-8912-5779
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Maxs U. E. Sanam(2)
Veterinary Diseases and Public Health Department, Faculty of Medicine and Veterinary Medicine, Nusa Cendana University
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Elisabet Tangkonda(3)
Veterinary Diseases and Public Health Department, Faculty of Medicine and Veterinary Medicine, Nusa Cendana University
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Yohanes T. R. M. R. Simarmata(4)
Clinic, Reproduction, Pathology and Nutrition Department, Faculty of Medicine and Veterinary Medicine, Nusa Cendana University
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Theresia F. I. M. D. Murni(5)
Tarus Animal Health Centre, Animal Husbandry Office of Kupang Regency
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Jois M. Jacob(6)
Animal Health Study Programme, Department of Animal Husbandry, Kupang State Agricultural Polytechnic
(*) Corresponding Author
Keywords:
African Swine Fever, virus ASF, babi, Kupang, Nusa Tenggara Timur, PCR
Abstract
African Swine Fever (ASF) adalah infeksi virus penting pada babi yang belakangan ini muncul kembali sebagai penyakit lintas batas yang menyebar dengan luas dan cepat. Konfirmasi pertama ASF tercatat di Indonesia pada akhir tahun 2019 dan kemudian meluas ke beberapa daerah termasuk Nusa Tenggara Timur (NTT). Informasi tentang virus ASF di NTT terbatas hingga saat ini. Studi ini bertujuan untuk mengidentifikasi keberadaan virus ASF pada kasus kematian massal babi di Kupang, Pulau Timor. Sampel ginjal dan limpa diambil dari delapan ekor babi yang mati mendadak di daerah Kupang. Teknik PCR konvensional dilakukan untuk mengidentifikasi daerah yang sangat terkonservasi dari genom virus ASF VP72. Hasil PCR menunjukkan bahwa semua sampel menampilkan panjang pita yang sesuai dengan pita target dari primer yang digunakan. Hasil ini menunjukkan bahwa virus ASF masih beredar di wilayah Kupang dan tetap menjadi ancaman bagi peternakan babi di daerah tersebut. Oleh karena itu, usaha untuk meningkatkan kesadaran publik terutama di kalangan produsen babi mengenai pentingnya penerapan biosekuriti harus dilakukan secara konsisten.
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References
Aguero, M., J. Fernandez, L. Romero, C. Sanchez Mascaraque, M. Arias, & J. M. Sanchez-Vizcaino. (2003). Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples. J Clin Microbiol 41(9):4431-4434. doi: 10.1128/jcm.41.9.4431-4434.2003
Alonso, C., M. Borca, L. Dixon, Y. Revilla, F. Rodriguez, J. M. Escribano, & I.R. Consortium. (2018). ICTV Virus Taxonomy Profile: Asfarviridae. J Gen Virol 99(5):613-614. doi: 10.1099/jgv.0.001049
Arimurti, P. I., Basri, C., & Lukman, D. W. (2021). Deteksi virus african swine fever dari sampah makanan kapal laut internasional di Pelabuhan Tanjung Priok. Acta Veterinaria Indonesiana 9(2): 112-119. doi:10.29244/avi.9.2.112-119
Beltrán-Alcrudo, D., M. Arias, C. Gallardo, S. A. Kramer, & M.-L. Penrith, editors. (2017). African swine fever: detection and diagnosis – A manual for veterinarians. Food and Agriculture Organization of the United Nations (FAO), pp:88
Blome, S., Franzke, K., & Beer, M. (2020). African swine fever - A review of current knowledge. Virus research 287:198099.doi:10.1016/j.virusres.2020.198099
BPS. (2020). Populasi Babi menurut Provinsi (Ekor), 2018-2020.https://www.bps.go.id/indicator/24/474/1/populasi-babi-menurut-provinsi.html.
Dharmayanti, N. I., Sendow, I., Ratnawati, A., Settypalli, T. B. K., Saepulloh, M., Dundon, W. G., Nuradji, H., Naletoski, I., Cattoli, G., & Lamien, C. E. (2021). African swine fever in North Sumatra and West Java provinces in 2019 and 2020, Indonesia. Transboundary and emerging diseases 68(5):2890–2896.doi:10.1111/tbed.14070
DISNAKPROVNTT. (2020). Menahan Laju Penyebaran Virus ASF di NTT.https://disnak.nttprov.go.id/2020/08/10/menahan-laju-penyebaran-virus-asf-di-ntt/
Dixon, L. K., Stahl, K., Jori, F., Vial, L., & Pfeiffer, D. U. (2020). African swine fever epidemiology and control. Annual review of animal biosciences 8(1):221-246. doi:10.1146/annurev-animal-021419-083741
Gallardo, C., P. Sastre, P. Rueda, A. P. Gerilovych, M. Kit, I. Nurmoja, & M. F. L. Potier. (2021). 5. Methods for African swine fever diagnosis in clinical and environmental samples, Understanding and combatting African Swine Fever. Wageningen Academic Publishers. p. 141-160
Ito, S., Kawaguchi, N., Bosch, J., Aguilar-Vega, C., & Sánchez-Vizcaíno, J. M. (2023). What can we learn from the five-year African swine fever epidemic in Asia?. Frontiers in veterinary science 10: 1273417.doi:10.3389/fvets.2023.1273417
Johns, C., C. Cargill, I. Patrick, M. Geong, J. Ly, &D. Shearer. (2009). Smallholder commercial pig production in NTT–opportunities for better market integration, SADI Final Report. Australian Centre for International Agricultural Research, Canberra, Australia
Kedkovid, R., C. Sirisereewan, & R. Thanawongnuwech. (2020). Major swine viral diseases: an Asian perspective after the African swine fever introduction. Porcine Health Management 6(1):20. doi: 10.1186/s40813-020-00159-x
Khoo, C.K., Norlina, D., Roshaslinda, D., Siti Suraya Hani, M.S., Zunaida, B., Mohd Hasrul, A.H., Pauzi, N.A.S., Roslina, H., Faizah Hanim, M.S. & Leow, B.L. (2021). African swine fever in backyard pigs of Sabah state, East Malaysia. Tropical Biomedicine 38(4): 499-504.doi:10.47665/tb.38.4.095
Kim, H. J., Cho, K. H., Lee, S. K., Kim, D. Y., Nah, J. J., Kim, H. J., Kim, H. J., Hwang, J. Y., Sohn, H. J., Choi, J. G., Kang, H. E., & Kim, Y. J. (2020). Outbreak of African swine fever in South Korea, 2019. Transboundary and emerging diseases 67(2):473–475.doi: 10.1111/tbed.13483
Kurniawan, R. E. (2024). Indonesia’s Updates on African Swine Fever in Domestic Swine PopulationReport. In WOAH Asia Regional Training on Swine Disease Diagnosis (pp. 1–19).
Leslie, E. E., Geong, M., Abdurrahman, M., Ward, M. P., & Toribio, J. A. L. (2015). A description of smallholder pig production systems in eastern Indonesia. Preventive veterinary medicine 118(4):319-327.doi: 10.1016/j.prevetmed.2014.12.006
Netherton, C. L., S. Connell, C. T. O. Benfield, & L. K. Dixon. (2019). The Genetics of Life and Death: Virus-Host Interactions Underpinning Resistance to African Swine Fever, a Viral Hemorrhagic Disease. Frontiers in Genetics 10(402).doi: 10.3389/fgene.2019.00402
Nguyen, H. Q., Nguyen, D. M. T., Nguyen, N. M., Nguyen, D. N. T., Luu, H. Q. T., & Do, D. T. (2021). Genetic analysis of African swine fever virus based on major genes encoding p72, p54 and p30. The Journal of Agriculture and Development 20(3):18-25. doi:10.52997/jad.3.03.2021
Nguyen, M.N., Ngo, T.T., Nguyen, D.M., Lai, D.C., Nguyen, H.N., Nguyen, T.T., Lee, J.Y., Nguyen, T.T. & Do, D.T. (2022). Genetic characterization of African swine fever virus in various outbreaks in central and southern Vietnam during 2019–2021. Current microbiology 79(11):341.doi: 10.1007/s00284-022-03033-x
Penrith, M. L., Okoth, E., & Livio Heath (2023). Special Issue "African Swine Fever and Other Swine Viral Diseases in Africa". Viruses 15(7): 1438. doi:10.3390/v15071438
Sawford, K., C. Robertson, S. Gunawardena, & C. Stephen. (2011). Development and application of a framework for emerging infectious disease intelligence in lower resource settings. J Bioterr Biodef 4(001):2-9.doi: 10.4172/2157-2526.S4-001
Sendow, I., Ratnawati, A., Dharmayanti, N. L. P., & Saepulloh, M. (2020). African Swine Fever: Penyakit Emerging yang Mengancam Peternakan Babi di Dunia. Indonesian Bulletin of Animal and Veterinary Sciences 30(1):15.doi: 10.14334/wartazoa.v30i1.2479
Spickler, A. R. (2019). African Swine Fever.http://www.cfsph.iastate.edu/DiseaseInfo/factsheets.php
Tenaya, W. M., Swacita, I. B. N., Wirata, K., Damriyasa, M., Besung, N. K., Suarsana, N., Sari, T. K., & Agustina, K. K. (2023). A study of African swine fever virus in Regional VI of the Disease Investigation Center of Denpasar Bali in Indonesia. Veterinary world 16(4):844–850.doi: 10.14202/vetworld.2023.844-850
Torresi, C., M. Fiori, L. Bertolotti, M. Floris, B. Colitti, M. Giammarioli, S. Dei Giudici, A. Oggiano, M. Malmberg, G. M. De Mia, S. Belák, & F. Granberg. (2020). The evolution of African swine fever virus in Sardinia (1978–2014) as revealed by whole-genome sequencing and comparative analysis. Transboundary and emerging diseases 67: 1971–1980. doi:10.1111/tbed.13540
Zhou, X., Li, N., Luo, Y., Liu, Y., Miao, F., Chen, T., Zhang, S., Cao, P., Li, X., Tian, K., Qiu, H. J., & Hu, R. (2018). Emergence of African Swine Fever in China, 2018. Transboundary and emerging diseases 65(6):1482–1484. doi:10.1111/tbed.12989.
Alonso, C., M. Borca, L. Dixon, Y. Revilla, F. Rodriguez, J. M. Escribano, & I.R. Consortium. (2018). ICTV Virus Taxonomy Profile: Asfarviridae. J Gen Virol 99(5):613-614. doi: 10.1099/jgv.0.001049
Arimurti, P. I., Basri, C., & Lukman, D. W. (2021). Deteksi virus african swine fever dari sampah makanan kapal laut internasional di Pelabuhan Tanjung Priok. Acta Veterinaria Indonesiana 9(2): 112-119. doi:10.29244/avi.9.2.112-119
Beltrán-Alcrudo, D., M. Arias, C. Gallardo, S. A. Kramer, & M.-L. Penrith, editors. (2017). African swine fever: detection and diagnosis – A manual for veterinarians. Food and Agriculture Organization of the United Nations (FAO), pp:88
Blome, S., Franzke, K., & Beer, M. (2020). African swine fever - A review of current knowledge. Virus research 287:198099.doi:10.1016/j.virusres.2020.198099
BPS. (2020). Populasi Babi menurut Provinsi (Ekor), 2018-2020.https://www.bps.go.id/indicator/24/474/1/populasi-babi-menurut-provinsi.html.
Dharmayanti, N. I., Sendow, I., Ratnawati, A., Settypalli, T. B. K., Saepulloh, M., Dundon, W. G., Nuradji, H., Naletoski, I., Cattoli, G., & Lamien, C. E. (2021). African swine fever in North Sumatra and West Java provinces in 2019 and 2020, Indonesia. Transboundary and emerging diseases 68(5):2890–2896.doi:10.1111/tbed.14070
DISNAKPROVNTT. (2020). Menahan Laju Penyebaran Virus ASF di NTT.https://disnak.nttprov.go.id/2020/08/10/menahan-laju-penyebaran-virus-asf-di-ntt/
Dixon, L. K., Stahl, K., Jori, F., Vial, L., & Pfeiffer, D. U. (2020). African swine fever epidemiology and control. Annual review of animal biosciences 8(1):221-246. doi:10.1146/annurev-animal-021419-083741
Gallardo, C., P. Sastre, P. Rueda, A. P. Gerilovych, M. Kit, I. Nurmoja, & M. F. L. Potier. (2021). 5. Methods for African swine fever diagnosis in clinical and environmental samples, Understanding and combatting African Swine Fever. Wageningen Academic Publishers. p. 141-160
Ito, S., Kawaguchi, N., Bosch, J., Aguilar-Vega, C., & Sánchez-Vizcaíno, J. M. (2023). What can we learn from the five-year African swine fever epidemic in Asia?. Frontiers in veterinary science 10: 1273417.doi:10.3389/fvets.2023.1273417
Johns, C., C. Cargill, I. Patrick, M. Geong, J. Ly, &D. Shearer. (2009). Smallholder commercial pig production in NTT–opportunities for better market integration, SADI Final Report. Australian Centre for International Agricultural Research, Canberra, Australia
Kedkovid, R., C. Sirisereewan, & R. Thanawongnuwech. (2020). Major swine viral diseases: an Asian perspective after the African swine fever introduction. Porcine Health Management 6(1):20. doi: 10.1186/s40813-020-00159-x
Khoo, C.K., Norlina, D., Roshaslinda, D., Siti Suraya Hani, M.S., Zunaida, B., Mohd Hasrul, A.H., Pauzi, N.A.S., Roslina, H., Faizah Hanim, M.S. & Leow, B.L. (2021). African swine fever in backyard pigs of Sabah state, East Malaysia. Tropical Biomedicine 38(4): 499-504.doi:10.47665/tb.38.4.095
Kim, H. J., Cho, K. H., Lee, S. K., Kim, D. Y., Nah, J. J., Kim, H. J., Kim, H. J., Hwang, J. Y., Sohn, H. J., Choi, J. G., Kang, H. E., & Kim, Y. J. (2020). Outbreak of African swine fever in South Korea, 2019. Transboundary and emerging diseases 67(2):473–475.doi: 10.1111/tbed.13483
Kurniawan, R. E. (2024). Indonesia’s Updates on African Swine Fever in Domestic Swine PopulationReport. In WOAH Asia Regional Training on Swine Disease Diagnosis (pp. 1–19).
Leslie, E. E., Geong, M., Abdurrahman, M., Ward, M. P., & Toribio, J. A. L. (2015). A description of smallholder pig production systems in eastern Indonesia. Preventive veterinary medicine 118(4):319-327.doi: 10.1016/j.prevetmed.2014.12.006
Netherton, C. L., S. Connell, C. T. O. Benfield, & L. K. Dixon. (2019). The Genetics of Life and Death: Virus-Host Interactions Underpinning Resistance to African Swine Fever, a Viral Hemorrhagic Disease. Frontiers in Genetics 10(402).doi: 10.3389/fgene.2019.00402
Nguyen, H. Q., Nguyen, D. M. T., Nguyen, N. M., Nguyen, D. N. T., Luu, H. Q. T., & Do, D. T. (2021). Genetic analysis of African swine fever virus based on major genes encoding p72, p54 and p30. The Journal of Agriculture and Development 20(3):18-25. doi:10.52997/jad.3.03.2021
Nguyen, M.N., Ngo, T.T., Nguyen, D.M., Lai, D.C., Nguyen, H.N., Nguyen, T.T., Lee, J.Y., Nguyen, T.T. & Do, D.T. (2022). Genetic characterization of African swine fever virus in various outbreaks in central and southern Vietnam during 2019–2021. Current microbiology 79(11):341.doi: 10.1007/s00284-022-03033-x
Penrith, M. L., Okoth, E., & Livio Heath (2023). Special Issue "African Swine Fever and Other Swine Viral Diseases in Africa". Viruses 15(7): 1438. doi:10.3390/v15071438
Sawford, K., C. Robertson, S. Gunawardena, & C. Stephen. (2011). Development and application of a framework for emerging infectious disease intelligence in lower resource settings. J Bioterr Biodef 4(001):2-9.doi: 10.4172/2157-2526.S4-001
Sendow, I., Ratnawati, A., Dharmayanti, N. L. P., & Saepulloh, M. (2020). African Swine Fever: Penyakit Emerging yang Mengancam Peternakan Babi di Dunia. Indonesian Bulletin of Animal and Veterinary Sciences 30(1):15.doi: 10.14334/wartazoa.v30i1.2479
Spickler, A. R. (2019). African Swine Fever.http://www.cfsph.iastate.edu/DiseaseInfo/factsheets.php
Tenaya, W. M., Swacita, I. B. N., Wirata, K., Damriyasa, M., Besung, N. K., Suarsana, N., Sari, T. K., & Agustina, K. K. (2023). A study of African swine fever virus in Regional VI of the Disease Investigation Center of Denpasar Bali in Indonesia. Veterinary world 16(4):844–850.doi: 10.14202/vetworld.2023.844-850
Torresi, C., M. Fiori, L. Bertolotti, M. Floris, B. Colitti, M. Giammarioli, S. Dei Giudici, A. Oggiano, M. Malmberg, G. M. De Mia, S. Belák, & F. Granberg. (2020). The evolution of African swine fever virus in Sardinia (1978–2014) as revealed by whole-genome sequencing and comparative analysis. Transboundary and emerging diseases 67: 1971–1980. doi:10.1111/tbed.13540
Zhou, X., Li, N., Luo, Y., Liu, Y., Miao, F., Chen, T., Zhang, S., Cao, P., Li, X., Tian, K., Qiu, H. J., & Hu, R. (2018). Emergence of African Swine Fever in China, 2018. Transboundary and emerging diseases 65(6):1482–1484. doi:10.1111/tbed.12989.
Published
2025-06-25
How to Cite
Gelolodo, M., Sanam, M., Tangkonda, E., Simarmata, Y., Murni, T., & Jacob, J. (2025). Molecular Identification of African Swine Fever Virus from an Outbreak in Kupang Region. JURNAL KAJIAN VETERINER, 13(1), 40-46. https://doi.org/10.35508/jkv.v13i1.19609
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