Analisis Aktivitas Nitrifikasi dari Bakteri yang diisolasi dari Rumah Burung Walet Menggunakan Metode Spektrofotometri
Analysis of Nitrification Activity of Bacteria Isolated from Swiftlet Houses Using the Spectrophotometric Method
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Palestin Palestin(1)
Faculty of Veterinary Medicine, Universitas Wijaya Kusuma Surabaya
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Siti Gusti Ningrum(2*)
Faculty of Veterinary Medicine, Universitas Wijaya Kusuma Surabaya
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Hana Cipka Pramuda Wardhani(3)
Faculty of Veterinary Medicine, Universitas Wijaya Kusuma Surabaya
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Dyan Nugrahadi Sutriatmaja(4)
Program Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya
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Charisma Putri Winda(5)
Program Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya
(*) Corresponding Author
Abstract
Nitrifying bacteria are essential in the nitrogen cycle, turning ammonia into nitrite and nitrate, which greatly contributes to the contamination of edible bird's nests in swiftlet houses. This study aims to evaluate the nitrifying activity of bacteria isolated from swiftlet housing environments in Central Kalimantan, Indonesia. A total of 16 environmental pooling samples were obtained from four different environmental sources within the swiftlet houses: faeces (n=4), pond water (n=4), edible bird’s nests (n=4), and soil (n=4). The nitrifying activity of each isolate was examined by measuring nitrite production using a spectrophotometric method and ammonium consumption using a commercial ammonium test kit. The main criteria investigated were the ability of the bacteria to oxidize ammonia to nitrite and their ability to result in ammonium reduction. The findings indicated that all 16 isolates were able to generate nitrite within a range of 4.8 to 24.7 ppm and showed considerable ammonium consumption. Among the sources, the bacteria from soil exhibited higher nitrifying activity compared to those from soil, pond water and bird’s nests. The findings indicate that the swiftlet house environment harbors diverse and active nitrifying bacteria. Furthermore, spectrophotometry method can be used as an initial detection for nitrifying bacteria.
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References
Chaali M, Ortiz HAR, Cano BD, Brar SK, Ramirez AA, Arriaga S, Heitz M. 2021. Immobilization of nitrifying bacteria on composite based on polymers and eggshells for nitrate production. Journal of bioscience and bioengineering 131(6): 663-670.
Fadillah H, Junaidi M, Azhar F. 2022. Penggunaan Nitrosomonas dan Nitrobacter untuk perbaikan kualitas air media budidaya ikan nila (Oreochromis niloticus). Jurnal Perikanan Unram 12(1): 54-65.
Hayatsu M, Katsuyama C, Tago K. 2021. Overview of recent researches on nitrifying microorganisms in soil. Soil Science and Plant Nutrition 67(6): 619-632.
Karuriya S, Bhandari P, Choudhary S. 2024. Diversity and function of nitrogen cycling microorganisms in mining-impacted areas and their potential role in biorestoration and wastewater treatment. In Emerging innovative trends in the application of biological processes for industrial wastewater treatment Elsevier. Pp. 165-187.
Medriano CA, Chan A, De Sotto R, Bae S. 2023. Different types of land use influence soil physiochemical properties, the abundance of nitrifying bacteria, and microbial interactions in tropical urban soil. Science of the Total Environment 869: 161722.
Mudrikah S, Hidayah H, Amelia T, Helsen H. 2024. Perbandingan Metode Analisis Instrumen HPLC dan Spektrofotometer UV-VIS. Jurnal Ilmiah Wahana Pendidikan 10(13): 377-386.
Ningrum SG. 2021. Deteksi kandungan nitrit dan hidrogen peroksida dalam produk sarang burung walet bersih asal Indonesia. Jurnal Ilmiah Kedokteran Wijaya Kusuma 10(1): 20-26.
Ningrum SG, Palgunad BU, Sasmita R. 2022. Evaluation of nitrite concentration in edible bird’s nest (white, yellow, orange, and red blood). Makara Journal of Science 26(1): 7.
Normaulidia N, Saidy AR, Yusran FH. 2022. Nitrogen availability in upland soil treated with swallow dropping. Acta Solum 1(1): 28-35.
Prasasti FE, Ningrum SG. 2024. Isolasi dan identifikasi bakteri nitrifikasi pada sarang burung walet (Aerodramus maximus). In Prosiding Seminar Nasional Kusuma, Surabaya October. 272-282.
Sulistiyowati H, Ali MSS, Mujahidin IF, Saud MY. 2024. Edible bird nest farming business development strategy in the Pulang Pisau Regency, Central Kalimantan Province, Indonesia. In IOP Conference Series: Earth and Environmental Science, May 012093.
Swardiani NPI, Swasta IBJ, Amelia JM, Antara KL. 2022. Studi perbandingan kualitas air pada sistem resirkulasi antara sistem yang menggunakan tanaman kangkung dan tanpa tanaman kangkung dilihat dari variabel amonia (NH3), nitrit (NO2), nitrat (NO3). Jurnal Perikanan Unram, 12(3), 355-364.
Vijayan A, Vattiringal Jayadradhan RK, Pillai D, Prasannan Geetha P, Joseph V, Isaac Sarojini BS. 2021. Nitrospira as versatile nitrifiers: taxonomy, ecophysiology, genome characteristics, growth, and metabolic diversity. Journal of Basic Microbiology 61(2): 88-109.
Wahyuni DS, Latif H, Sudarwanto MB, Basri C. 2022. Pola pemeliharaan burung walet pada pulau-pulau utama penghasil sarang burung walet di Indonesia. Jurnal Sain Veteriner 40(2): 117-127.
Widiyani P, Latif H, Lukman DW, Sudarwanto MB. 2021. Artikel review: bakteri nitritasi dan peranannya dalam keberadaan nitrit pada sarang burung walet. Jurnal Kajian Veteriner 9(2): 98-109.
Widiyani P, Sudarwanto MB, Latif H, Lukman DW, Thong D, Rahayu P. 2022. A preliminary metagenomics study of bacteria present in the dirt of swiftlet farmhouses based on nitrite levels in edible bird’s nest on Sumatera Island, Indonesia. Veterinary World 15(7): 1798.
Zulkefle NN, Ibrahim MA, Azuan NF, Ch’ng SE, Ismail N, Abu-Bakar MZ, Chan KW. 2024. A review on the edible bird’s nest quality and manufacturing standards of the three largest exporting countries in the world. Journal of Food Quality 2024(1): 5608357.
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