Genetic Variation Analysis of FSHB Gene Exon 3 in Goats (Capra hircus) Using in Silico Restriction Fragment Length Polymorphism (RFLP)
Analisis Variasi Genetik Ekson 3 Gen FSHB pada Kambing (Capra hircus) Menggunakan Restriction Fragment Length Polymorphism (RFLP) Secara in Silico
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Hendro Sukoco(1*)
Department Of Animal Husbandry, Faculty Of Animal Husbandry dan Fisheries, Universitas Sulawesi Barat, Majene, Indonesia.
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Hermilinda Parera(2)
Kupang State Agricultural Polytrchnic Animal health Study Program. Jl. Prof. Dr. Herman Yohanes. Lasiana Kupang, Indonesia.
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Victor Lenda(3)
Kupang State Agricultural Polytrchnic Animal health Study Program. Jl. Prof. Dr. Herman Yohanes. Lasiana Kupang, Indonesia.
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Nancy Diana Foeh(4)
Laboratory of Clinical, Reproduction, Pathology and Nutrition, Faculty of Medicine and Veterinary Medicine, University on Nusa Cendana, Kupang.
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Dihan Kurnia(5)
Animal Production Technology Study Program, Agricultural Polytechnic Payakumbuh, Lima Puluh Kota 26271. Country: Indonesia
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Dwi Nurhayati(6)
Department of Animal Husbandry, Faculty of Animal Husbandry, University of Papua, Manokwari, West Papua 98314, Indonesia
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Muhammad Mirandy Pratama Sirat(7)
Study Program of Animal Nutrition and Feed Technology, Department of Animal Husbandry, Faculty of Agriculture, Universitas Lampung
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Ratna Ermawati(8)
Study Program of Animal Husbandry, Department of Animal Husbandry, Faculty of Agriculture, Universitas Lampung Country: Indonesia
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Made Bagus Erlangga(9)
Department Of Reproduction and Obstetrics, Faculty Of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia
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Salmin -(10)
Department Of Animal Husbandry, Faculty of Animal Husbandry and Fisheries, Tadulako University, Indonesia
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Annisa Putri Cahyani(11)
Animal Production Technology Study Program, Polytechnic of Agricultural Development Yogyakarta Magelang, Indonesia
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Ferbian Milas Siswanto(12)
Department of Chemistry and Biochemistry, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia.
(*) Corresponding Author
Keywords:
BspMAI/PstI, FSHB gene, goat, restriction pattern, RFLP in silico
Abstract
Studi ini bertujuan untuk melakukan analisis in silico awal Restriction Fragment Length Polymorphism (RFLP) pada ekson 3 gen Hormon Perangsang Folikel Beta (FSHB) pada kambing (Capra hircus) menggunakan enzim restriksi BspMAI dan PstI. Empat sekuens ekson 3 gen FSHB yang telah dipublikasikan, masing-masing sepanjang 310 bp, diperoleh dari basis data NCBI dan dianalisis dengan digesti restriksi virtual. Hasil analisis memprediksi dua pola restriksi pada sekuens yang dianalisis: pola tidak terpotong sepanjang 310 bp dan pola terpotong yang menghasilkan fragmen sepanjang 200 bp dan 110 bp. Pola tidak terpotong sepanjang 310 bp ditemukan pada tiga sekuens, sedangkan pola 200 bp dan 110 bp ditemukan pada satu sekuens. Hasil ini menunjukkan kemungkinan adanya perbedaan situs restriksi pada atau di sekitar situs pengenalan BspMAI/PstI pada ekson 3 gen FSHB kambing. Namun, karena penelitian ini terbatas pada analisis komputasional sekuens publik dan tidak melibatkan sampel biologis, amplifikasi PCR, pengurutan, pencernaan enzim restriksi langsung, atau validasi elektroforesis, temuan ini masih bersifat pendahuluan. Penelitian lebih lanjut menggunakan penyelarasan sekuens berganda, identifikasi SNP, dan validasi PCR-RFLP laboratorium pada populasi kambing sebenarnya diperlukan untuk mengkonfirmasi potensi penggunaan BspMAI/PstI dalam studi genetika kambing
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References
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Ali, A., Derar, D.R., & Elshahed, M. 2022. Management Factors Affecting Reproductive Performance and Causes of Infertility of Ardi Goats in Saudi Arabia. Journal of the Saudi Society of Agricultural Sciences. 21(2): 93-97
Andreas, F., Arifiantini, I., Saputra, F., Ishak, A.B.L., Imron, M., & Sumantri, C. 2014. Effect Of FSH Β-Sub Unit And FSHR Genes Polymorphisms On Superovulatory Response Traits. J. Indonesian Trop. Anim. Agric. 39(4):197-203
Brown, T.A. 2016. Gene Cloning and DNA Analysis: An Introduction. 7th ed. Chichester: Wiley-Blackwell.Dai, L., Zhao, Z., Zhao, R., Xiao, S., Jiangm H., Yue, X., Li, X., Gao, Y., Liu, J., & Zhang, J. 2009. Effects Of Novel Single Nucleotide Polymorphisms Of The FSH Beta-Subunit Gene On Semen Quality And Fertility In Bulls. Anim Reprod Sci. 114(1-3): 14-22
Das, N., & Kumar, T.R. 2018. Molecular Regulation of Follicle-Stimulating Hormone Synthesis, Secretion and Action. J Mol Endocrinol. 60(3):R131–R155
Dwatmadji., Suteky, T., & Sutrisno, E. 2018. Reproductive And Feeding Management To Improve The Performance Of Livestock In Tugu Rejo, Kabawetan District Kepahiang Bengkulu. Dharma Raflesia : Jurnal Ilmiah Pengembangan Dan Penerapan IPTEKS. 15(1): 29-36
Haldar, S., Agrawal, H., Saha, S., Straughn, A.R., Roy, P., & Kakar, S.S. 2022. Overview Of Follicle Stimulating Hormone And Its Receptors In Reproduction And In Stem Cells And Cancer Stem Cells. Int. J. Biol. Sci. 18: 675-692
Hathaway, L.J., Brugger, S., Martynova, A., Aebi, S., Mu¨hlemann, K. 2007. Use of the Agilent 2100 Bioanalyzer for Rapid and ReproducibleMolecular Typing of Streptococcus pneumoniae. Journal Of Clinical Microbiology. 45(3): 803-809.
Ishak, A.B.L., Sumantri, C., Noor, R.R., & Arifiantini, I. 2011. Identification Of Polymorphism Of FSH Beta-Subunit Gene As Sperm Quality Marker In Bali Cattle Using PCR-RFLP. J. Indonesian Trop. Anim. Agric. 36(4): 221-227
Janjic, M.M., Prévide, R.M., Fletcher, P.A., Sherman, A., Smiljanic, K., Abebe, D., Bjelobaba, I., Stojilkovic, S.S. 2019. Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo. Sci Rep. 9:20098
Mao, L., Wang, L., Bennett, S., Xu, J., & Zou, J. 2022. Effects Of Follicle-Stimulating Hormone on Fat Metabolism and Cognitive Impairment in Women During Menopause. Front Physiol. 13:1043237.
McDonald, R., Sadler, C., & Kumar, T.R. 2019. Gain–of–Function Genetic Models to Study FSH Action. Front. Endocrinol. 10:28
Maretta, D., Helianti, I., Purwoko, D., Santosa, E., & Sobir. 2025. Identification and In Silico Analysis of a Cellulose Synthase-Like Gene on Eddoe Taro. Sains Malaysiana. 54(3): 769-783
Mokhtari, Z., Ahmadi, A., Zamani, P., Talebi, R., Ghaffari, M.R. 2021. Association of novel polymorphisms in follicle stimulating hormone beta (FSHβ) gene with litter size in Mehraban sheep. Journal Of Livestock Science and Technologies. 9(2): 21-29
Nikbin, S., Panandam, J.M., Yaakub, H., & Murugaiyah. 2013. Association FSHB Gene Polymorphisms With Semen Quality Traits In Boer Goats. Available athttps://www.ncbi.nlm.nih.gov/nuccore?term=popset+representative+uid+544207170%5Bword%5D&cmd=DetailsSearch. Retrieved on 08-08-2025.
Santi, D., Crepieux, P., Reiter, E., Spaggiari, G., Brigante, G., Casarini, L., Rochira, V., & Simoni, M. 2020. J Clin Med. 9(4): 1014.
Volkandari, S.D., Kakisina, P., Rumanta, M., & Kunda, R.M. 2019. Genetic Identification of POU1F1|PstI gene of Lakor Goat from Lakor Island, Southwest Maluku Regency. IOP Conf. Ser.: Earth Environ. Sci. 387 012123
Wang, H.-Q., Zhang, W.-D., Yuan, B., & Zhang, J.-B. 2021. Advances in the Regulation of Mammalian Follicle-Stimulating Hormone Secretion. Animals. 11(4): 1134
Zhang, C.Y., Wu, C.J., Zeng, W.B., Huang, K.K., Li, X., Feng, J.H., Wang, D., Hua, G.H., Xu, D.O., Wen, Q.Y., & Yang, L.G. 2011. Polymorphism In Exon 3 Of Follicle Stimulating Hormone Beta (FSHB) Subunit Gene And Its Association With Litter Traits And Superovulation In The Goat. Small Ruminant Research. 96: 53–57.
Published
2026-06-13
How to Cite
Sukoco, H., Parera, H., Lenda, V., Foeh, N., Kurnia, D., Nurhayati, D., Sirat, M., Ermawati, R., Erlangga, M., -, S., Cahyani, A., & Siswanto, F. (2026). Genetic Variation Analysis of FSHB Gene Exon 3 in Goats (Capra hircus) Using in Silico Restriction Fragment Length Polymorphism (RFLP). JURNAL KAJIAN VETERINER, 14(1), 40-50. https://doi.org/10.35508/jkv.v14i1.25855
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Articles
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