Identifikasi Keberadaan Gen mecA, mecC dan blaZ pada Isolat Tersimpan Staphylococcus aureus Asal Susu Sapi Perah Mastitis Subklinis
Identification of mecA, mecC and blaZ Genes in Stored Staphylococcus aureus Isolates from Cow Milk with Subclinical Mastitis
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Ghina Nazalia(1)
Program Studi Kedokteran Hewan, Fakultas Kedokteran, Universitas Padjadjaran, Sumedang, Jawa Barat, 45363, Indonesia
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Sarasati Windria(2)
Departemen Ilmu Kedokteran Dasar, Divisi Mikrobiologi, Fakultas Kedokteran, Universitas Padjadjaran, Sumedang, Jawa Barat, 45363, Indonesia
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Adi Imam Cahyadi(3*)
Departemen Ilmu Kedokteran Dasar, Divisi Mikrobiologi, Fakultas Kedokteran, Universitas Padjadjaran, Sumedang, Jawa Barat, 45363, Indonesia
(*) Corresponding Author
Abstract
Mastitis is an inflammation of the bovine udder that can reduce both the quantity and quality of milk. This condition is commonly caused by Staphylococcus aureus which has the ability to develop resistance to antibiotics. The most frequently reported resistance is associated with methicillin-resistant Staphylococcus aureus (MRSA), with the possible involvement of β-lactamase enzymes. This study aimed to identify resistance-encoding genes, namely mecA, mecC, and blaZ, in stored Staphylococcus aureus isolates obtained from the milk of dairy cows with subclinical mastitis. The study was conducted using phenotypic and genotypic approaches to reconfirm the species of Staphylococcus aureus. The identification results showed that 22 of 28 isolates (78,6%) were confirmed as Staphylococcus aureus. Antibiotic susceptibility testing using the Kirby–Bauer method showed that 2 of 22 isolates (9,1%) were resistant to penicillin G, while all isolates (100%) remained sensitive to cefoxitin. PCR results showed that 5 of 22 isolates (22,7%) carried the blaZ gene, whereas none of the isolates (0%) were detected to carry the mecA or mecC genes. These findings indicate that the resistance mechanism in stored Staphylococcus aureus isolates from dairy cows with subclinical mastitis in Tanjungsari District is associated with β-lactamase production.
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