Kualitas Semen Beku Babi Peranakan Landrace pada Berbagai Level Madu Dalam Pengencer Tris-Kuning Telur
Quality of Frozen Semen of Landrace Crossbred Boars at Different Levels of Honey in a Tris–Egg Yolk Extender
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Maria Meldiana Jemamu(1*)
Fakultas Peternakan, Kelautan dan Perikanan, Universitas Nusa Cendana
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Wilmientje Marlene Nalley(2)
Fakultas Peternakan, Kelautan dan Perikanan, Universitas Nusa Cendana
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Yustiany Yuliana Bette(3)
Fakultas Peternakan, Kelautan dan Perikanan, Universitas Nusa Cendana
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Thomas Mata Hine(4)
Fakultas Peternakan, Kelautan dan Perikanan, Universitas Nusa Cendana
(*) Corresponding Author
Abstract
Information regarding the optimal concentration of honey in Tris-egg yolk extender to maintain the quality of frozen boar semen remains limited. This study aimed to evaluate the effect of honey supplementation on the quality of frozen semen of landrace crossbred boars. Honey was selected due to its antioxidant properties and its role as an energy source (glucose and fructose), which may protect spermatozoa from cryodamage during the freezing process. This experiment applied a completely randomized design with five treatments and four replications. Semen was collected from four landrace crossbred boar aged -2,5 years with ≥75% motility and ≤20% abnormalities. The treatments were T0 (control without honey), T1 (0,75%), T2 (1,50%), T3 (2,25%), T4 (3%) honey in Tris-egg yolk extender. Observed parameters included motility, viability, abnormality and recovery rate after thawing. Statistical analysis showed that honey supplementation significantly affected (P<0,05) semen quality. The best result was obtained at 2,25% honey (T3),with post-thaw motility of 42,35%, viability of 59,80%, abnormality of 10,11%, and recovery rate of 52,64%. It can be concluded that 2,25% honey supplementation in Tris-egg yolk extender is the optimal concentration for maintaining the quality of frozen semen of landrace crossbred boars.
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References
Banday, M. N, Lone, F. A., Rasool, F., Rather, H. A., dan Rather, M. A. 2017. Does natural honey act as an alternative to antibiotics in the semen extender for cryopreservation of crossbred ram semen?. Iranian journal of veterinary research, 18(4): 258-263.
Bucak, M. N., Keskin, N., Ili, P., Bodu, M., Akal, E., Başpınar, N., dan Coyan, K. 2020s. Effects of cryopreservation on sperm quality: Mechanisms of cryodamage and strategies to improve post-thaw sperm function. Theriogenology, 150, 47–56.
Chung, E. L. T., Nayan, N., Nasir, N. S. M., Hing, P. S. A., Ramli. S., Rahman, M. H. A., Kamalludin, M. H. 2019. Effect of honey as an additive for cryopreservation on bull semen quality from different cattle breeds uder tropical condition. Journal of Animal Health and Production, 7(4):171-178.
Dylanesia, W. 2023. Lebah Si Produsen Emas: Mengenal Lebih Jauh Tentang Madu Kehidupan Lebah. Cahaya Harapan. Yogyakarta.
Fraser, L., dan Strzeżek, J. 2020. Effects of cooling and freezing on boar sperm membranes and their functional consequences. Theriogenology, 150, 366–374.
Gardela, J., Conca, M. R., Palomares, Maneu, S. G., Calvo, G.D., Bejar, M. L., Pastor, F. M., Rodriguez, M. A. 2023. Effect of Honey, Coenzyme Q10, and β Carotene/α Tocopherol as Novel Additives in Rabbit Sperm Cryopreservation Extender. Animals, 13(14):23-29.
Giaretta, E., Bucci, D., Mari, G., Tamanini, C., dan Galeati, G. 2022. Sperm cryopreservation in pigs: Current status and future perspectives. Animals, 12(3), 300.Husna, A., Wahyuni, S., dan Susilawati, T. 2018. Pengaruh penambahan madu dalam pengencer terhadap kualitas semen beku. Jurnal Ilmu-Ilmu Peternakan, 28(2), 120–127.
Kamal, M. M., Ghoneim, I. M., dan El-Badry, D. A. 2022. Effects of tris (hydroxymethyl) aminomethane and egg yolk on semen extenders. Journal of Veterinary Medical Research, 33(2): 235-243.
Knox, R. V. 2020. Artificial insemination in pigs today: Current practices and future perspectives. Theriogenology, 150, 390–397.
Kumaresan, A., Johannisson, A., Humblot, P., dan Morrell, J. M. 2020. Sperm morphology and its relationship to fertility: A review. Theriogenology, 150, 407–414.
Layek, S. S., Mohanty, T. K., Kumaresan, A., dan Parks, J. E. 2024. Role of antioxidants in improving sperm function during cryopreservation. Theriogenology, 210, 45–53.
Maidin, M. S., Padlan, M. H., Azuan, S. A. N., Jonit, R., Mohammed, N. H., Abdullah, R. 2018. Supplemenentation of Niggela sativa oil and honey prolong the survival rate of fresh and post–thawed goat sperms. Journal Tropical Animal Science, 41(2):94-99.
Moussa, M., Abdel-Salaam, A., dan El-Harairy, M. A. 2025. Protective effect of low-density lipoprotein extracted from egg yolk on cryopreserved bull semen. Animals, 15(3): 411.
Nalley, D. V., Wila, I. B. W., dan Budiasa, M. K. 2023. Perbandingan Kualitas Sperma Babi Landrace dalam Pengencer Beltsville Thawing Solution, Mulberry III, dan Sperm Life yang Disimpan pada Suhu 18°C. Jurnal Veteriner, 24(3): 347-356.
Nasreen, S., Awan, M. A., UI-Husna, A., Rakha, B. A., Ansari, M. S., Holt, W., dan Akhter, S. 2020. Honey as an alternative to antibotics for cryopreservation of Nili-Ravi buffalo bull spermatozoa. Biopreservation and biobangking, 18(1): 25-32.
Olayemi, F. O., Adeniji, D. A., & Oyeyemi, M. O. 2020. Antioxidant properties of honey and its effects on sperm preservation. Andrologia, 52(5), e13545.
Pradana, W., dan Karja, N. W. K. 2021. Peran antioksidan dalam mempertahankan kualitas spermatozoa selama kriopreservasi. Jurnal Veteriner, 22(2), 150–158.
Priyanto, L., Susilawati, T., dan Wahyuningsih, S. 2020. Evaluasi viabilitas spermatozoa pasca pembekuan. Jurnal Veteriner, 21(3), 345–352.
Sari, D. P., Raharjo, S., dan Widayati, D. T. 2023. Interaksi krioprotektan terhadap membran spermatozoa dan implikasinya pada kualitas pasca thawing. Jurnal Ilmu Ternak dan Veteriner, 28(1), 25–33.
Yeste, M. 2021. Sperm cryopreservation update: Mechanisms of cryodamage and biomarkers. Reproduction in Domestic Animals, 56(S2), 3–13.
Yüksel, H., Eser, A., Arıcı, R., Yağcıoğlu, S., dan Ak, K. 2024. Pengaruh penambahan madu bunga dan madu pinus pada pengencer terhadap karakteristik spermatologi pada semen domba jantan. Jurnal Perhimpunan Dokter Hewan Yunani, 74(4): 6351–6360.
Zhu, X., Shi, W., Liu, X., Zhang, J. 2021. Efek perlindungan krioprotektan terhadap spermatozoa selama pembekuan: Sebuah tinjauan. Kriobiologi, 98: 66-67.
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