Motilitas Spermatozoa Babi pada Pengencer Kuning Telur Ayam Kampung dengan Penambahan Glukosa yang Disimpan pada Suhu Preservasi
Motility and Viability of Pig Spermatozoa in Chicken Egg Yolk Diluent with Addition of Glucose Stored at Preservation Temperature
-
Wilhelmina Afra Bala(1)
Program Studi Kedokteran Hewan, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana
-
Nancy D. F. K. Foeh(2*)
Bagian, Reproduksi, Patologi, dan Nutrisi, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana
-
Cynthia Dewi Gaina(3)
Bagian, Reproduksi, Patologi, dan Nutrisi, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana
(*) Corresponding Author
Abstract
Liquid semen is one way of preserving semen which is carried out for short storage purposes at a temperature of preservation. with the help of a diluent. boar semen is different from other ruminant semen, so it needs special handling. Storage of pig semen at cold temperatures can reduce the motility and viability of spermatozoa. The aim of this study was to maintain the quality of pig semen by adding coconut water and native chicken egg yolk diluent with the addition of glucose and stored at a temperature preservation. Boar semen was collected from sexually mature adult boar aged 2 years, the collection was carried out by the massage method. Semen that has been accommodated, is evaluated macroscopically and microscopically to see the quality of the cement, before dilution is carried out. This study was designed with a completely randomized design and factorial pattern with 2 control groups, 4 treatment groups, and 4 replications. The control group consisted of fresh semen without diluent. K0 The treatment groups were P1 (80CW + 20% NCEY + 20 L glucose), P2 (80% CW + 20% NCEY + 40 L glucose), P3 (80% CW + 20% NCEY + 60 L glucose) and P4 (80 % CW + 20% NCEY + 90 L glucose), which was preserved by the water jacket method. Spermatozoa motility and viability were evaluated every 2 hours until the motility value reached 40%. The results showed that P3 was the best combination to preserve cement (32 hours).
Downloads
References
Garner, D. L. And Hafez, E. S. E. (2000). Spermatozoa And Seminal Plasma. In: Hafez E. S. E. Editor. Reproduction In Farm Animals 7th. Ed. USA: Williams & Wilkins.
Mayesta, D. D. M., Trilaksana, I. N. B., & Bebas, W. (2014). Motilitas dan daya hidup spermatozoa ayam dalam pengencer glukosa kuning telur fosfat pada penyimpanan 3-5oC. Indonesia medicus Veterinus 3(1): 43-52.
Moussa, M., Martinet, V., Trimeche, A., Tainturier, D., & Anton, M. (2002). Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen–thawed bull semen. Theriogenology 57(6): 1695-1706.
Mukminat, A., & Suharyati, S. (2014). Pengaruh Penambahan Berbagai Sumber Karbohidrat pada Pengencer Skim Kuning Telur Terhadap Kualitas Semen Beku Sapi Bali. Jurnal Ilmiah Peternakan Terpadu 2(2).
Pamungkas, F. A., Mahmilia, F., & Elieser, S. (2008). Perbandingan karakteristik semen kambing Boer dengan Kacang. Pros. In Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor. Pp: 11-12.
Paulenz, H., Söderquist, L., Pérez-Pé, R., & Berg, K. A. (2002). Effect of different extenders and storage temperatures on sperm viability of liquid ram semen. Theriogenology 57(2): 823-836.
Rizal, M. (2006). Pengaruh penambahan laktosa di dalam pengencer tris terhadap kualitas semen Cair domba Garut. Jurnal Pengembangan Peternakan Tropis 31(1): 224-231.
Sumardani, N. L. G., Budaarsa, K., Putri, T. I., & Puger, A. W. (2019). Umur Memengaruhi volume semen dan motilitas spermatozoa babi landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali. Jurnal Veteriner 20(3): 324-329.
Susilawati, T. (2017). Spermatology Volume 2. Malang: Universitas Brawijaya Press.
Copyright (c) 2025 JURNAL KAJIAN VETERINER
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
.png)
