Uji Resistansi Pseudomonas aeruginosa terhadap Antibiotika pada Ikan Mas (Cyprinus carpio) di Kabupaten Kupang
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Peter Febriano Kapitan(1*)
Program Studi Kedokteran Hewan, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang, Indonesia
https://orcid.org/0009-0003-1714-6858
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Maxs Urias Ebenhaezer Sanam(2)
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang, Indonesia
https://orcid.org/0000-0001-9957-2946
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Elisabet Tangkonda(3)
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang, Indonesia
https://orcid.org/0000-0002-5811-7696
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Fhady Risckhy Loe(4)
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran dan Kedokteran Hewan, Universitas Nusa Cendana, Kupang, Indonesia
https://orcid.org/0009-0000-1969-1399
(*) Corresponding Author
Keywords:
Pseudomonas aeruginosa, Common carp, Antibiotic Resistance, Kupang Regency
Abstract
Common carp (Cyprinus carpio) is an important freshwater aquaculture commodity with high economic value; however, intensive farming practices increase the risk of bacterial diseases that can cause significant economic losses. One of the pathogenic bacteria frequently infecting common carp is Pseudomonas aeruginosa, while excessive and uncontrolled antibiotic use may contribute to bacterial resistance. This study aimed to analyze the resistance level of P. aeruginosa isolated from common carp against several antibiotics in Kupang Regency. Samples consisted of common carp showing clinical signs of bacterial infection, including reddish spots, scale detachment, and wounds on the body surface. Bacterial isolation was performed using Pseudomonas Agar Base (PAB), followed by identification through colony morphology, Gram staining, and biochemical tests comprising Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), and Simon’s Citrate Agar (SCA). Antibiotic susceptibility testing was conducted using the Kirby–Bauer disk diffusion method with gentamicin, ciprofloxacin, and chloramphenicol, and inhibition zones were interpreted according to Clinical and Laboratory Standards Institute (CLSI) standards. The results confirmed the presence of P. aeruginosa in infected common carp and revealed varying levels of susceptibility to the tested antibiotics. These findings indicate the occurrence of antibiotic resistance and emphasize the importance of prudent antibiotic selection and use in common carp health management.
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References
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Altaee, A.J., dan Al-Dabbagh, S.Y. 2022, 'Molecular Identification Of Virulence Genes Of Pseudomonas Aeruginosa Isolated From Fish (Cyprinus Carpio) In Mosul City', Iraqi Journal of Veterinary Sciences, 36 (4):953–958. https://doi.org/10.33899/ijvs.2022.132660.2119
Astutiwati. 2025, 'Pengaruh Padat Tebar Yang Berbeda Terhadap Pertumbuhan Dan Tingkat Kelangsungan Hidup Benih Ikan Bawal Bintang (Trachinotus Blochii)', Jupiter, 2 (1):1–10.
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Ciptaning Weargo Jati*), Huriyatul Fitriyah Noor(1), H.P.F., dan Jl. 2023, 'Efektivitas Vaksin Inaktif Aeromonas Salmonicida Terhadap Total Leukosit Dan Aktifitas Fagositosis Pada Ikan Koi (Cyprinus Carpio)' 5 (2):313–319. https://doi.org/https://doi.org/10.1099/00207713-22-2-53
Fajar, M.T. 2022, 'Pengaruh Perbedaan Konsentrasi Pakan Pelet Terhadap Bobot Dan Panjang Ikan Mas (Cyprinus Carpio)', INSOLOGI: Jurnal Sains dan Teknologi, 1 (5 SE-Articles):498–504. https://doi.org/10.55123/insologi.v1i5.801
Faragi, J.K. Al, dan Zakair, K.Y. 2022, 'Detection Of The Most Important Pathogenic Bacteria Affect External Organs Of Cyprinus Carpio In Wasit Province', Iraqi Journal of Agricultural Sciences, 53 (5):1115–1122.
Ghssein, G., dan Ezzeddine, Z. 2022, 'A Review Of Pseudomonas Aeruginosa Metallophores: Pyoverdine, Pyochelin And Pseudopaline', Biology, . https://doi.org/10.3390/biology11121711
Ichsyani, M., dan Nadira, N.R. 2024, 'Isolasi Dan Identifikasi Pseudomonas Aeruginosa Penyebab Infeksi Nosokomial Pada Permukaan Bowl Rinse Dental Unit RSGMP Universitas Jenderal Soedirman', Journal of Dental and Biosciences, 1 (1):1–7. https://doi.org/10.20884/1.j.dentbios.2024.1.01.10787
Ismail, dan Khumaidi, A. 2016, 'Teknik Pembenihan Ikan Mas (Cyprinus Carpio L) Di Balai Benih Ikan (BBI) Tenggarang Bondowoso', Samakia: Jurnal Ilmu Perikanan, 7 (1):27–37.
Juniawan, M.F., Artanti, D., Gayatri, Y., dan Ainutajriani, A. 2023, 'Isolasi Dan Karakterisasi Bakteri Termofilik Dari Oil Sludge Asal Kalimantan Timur', The Journal of Muhammadiyah Medical Laboratory Technologist, 6 (1):18–29. https://doi.org/10.30651/jmlt.v6i1.15898
Kementerian Kelautan dan Perikanan. 2024, 'Laporan Kinerja Direktorat Jendera Perikanan Budi Daya Tahun 2024'.
Kholil, I., Hossain, M.M., Neowajh, S., Islam, S., dan Kabir, M. 2015, 'Comparative Efficiency Of Some Commercial Antibiotics Against Pseudomonas Infection In Fish', International Journal of Fisheries and Aquatic Studies, 2 (3):114–117. https://doi.org/10.22271/fish.2015.v2.i3b.225
Ljubojević, P.D., Radosavljević, V., Pelić, M., Živkov Baloš, M., Puvača, N., Jug-Dujaković, J., dan Gavrilović, A. 2024, 'Antibiotic Residues In Cultured Fish: Implications For Food Safety And Regulatory Concerns', Fishes, . https://doi.org/10.3390/fishes9120484
Miller, A.R., dan Heather, H. 2018, 'Antimicrobial Drug Resistance In Fish Pathogens', Microbiology Spectrum, 6 (1):1–20. https://doi.org/10.1128/microbiolspec.arba-0017-2017
Mudaliar, S.B., Bharath, A.P., dan Srinivas. 2024, 'A Biomedical Perspective Of Pyocyanin From Pseudomonas Aeruginosa: Its Applications And Challenges.', World journal of microbiology & biotechnology, 40 (3):1–23. https://doi.org/10.1007/s11274-024-03889-0
Nasution, Z., Dewita, Y., dan Miftakhul, H. 2011, 'Perikanan Budidaya Di Danau Maninjau : Antisipasi Kebijakan Penanganan Dampak Kematian Masal Ikan' 1 (1):19–31.
Pratama, R.A., Djauhari, R., Monalisa, S.S., dan Susanti, W. 2023, 'IKAN AIR TAWAR Identification Of Bacterial In Several Types Of Freshwater Fish' 17.
Rahmadian, C.A., Ismail, Abrar, M., Erina, Rastina, dan Fahrimal, Y. 2018, 'Isolasi Dan Identifikasi Bakteri Pseudomonas Sp Pada Ikan Asin Di Tempat Pelangan Ikan Labuhanhaji Aceh Selatan', Jurnal Ilmiah Mahasiswa Veteriner, 2 (4):493–502. https://doi.org/https://doi.org/10.21157/jim%20vet..v2i4.9041
Rahmawati, L., Adlina, S., dan Yuliana, A. 2021, 'Isolasi Dan Identifikasi Bakteri Penghasil Protease Ekstraseluler Dari Limbah Cair Tahu Putih', Jurnal Kesehatan Bakti Tunas Husada : Jurnal Ilmu Ilmu Keperawatan, Analis Kesehatan dan Farmasi, 21 (2):187–193. https://doi.org/http://dx.doi.org/10.36465/jkbth.v21i2.748
Ramalho, R., Cunha, J., Teixeira, P., dan Gibbs, P.A. 2002, 'Modified Pseudomonas Agar: New Differential Medium For The Detection/Enumeration Of Pseudomonas Aeruginosa In Mineral Water.', Journal of microbiological methods, 49 (1):69–74. https://doi.org/10.1016/s0167-7012(01)00365-7
Riko, Yulianto, R., Wijanarko, R., dan Kurniawan, A. 2024, 'Pembenihan Ikan Mas Koki (Carrasius Auratus) Di Balai Benih Ikan (BBI) Nitikan, Yogyakarta', Amreta Meena, 1 (2):76–80. https://doi.org/doi.org/10.33019/am.v1i2.4917
Schmitz, F.-J., dan Fluit, A.C. 2010, 'Chapter 131 - Mechanisms Of Antibacterial Resistance', in: Cohen, J., Opal, S.M., Powderly, W.G.B.T.-I.D. (Third E. (Ed.), Infectious Diseases (Third Edition). , Mosby, London, hal. 1308–1322. https://doi.org/https://doi.org/10.1016/B978-0-323-04579-7.00131-3
Takril, Ansar, M., dan Sakia, N. 2021, 'Efektivitas Gel Lidah Buaya Dengan Dosis Berbeda Untuk Pengobatan Ikan Mas (Cyprinus Carpio) Terinfeksi Pseudomonas Aeruginosa', Journal of Fisheries and Marine Science, 2 (2):135–141. https://doi.org/https://doi.org/10.31605/siganus.v2i2.989
Published
2026-09-24
How to Cite
Kapitan, P., Sanam, M., Tangkonda, E., & Loe, F. (2026). Uji Resistansi Pseudomonas aeruginosa terhadap Antibiotika pada Ikan Mas (Cyprinus carpio) di Kabupaten Kupang. Jurnal Veteriner Nusantara, 9(2), 35-42. https://doi.org/10.35508/jvn.v9i2.28998
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Articles
Copyright (c) 2026 Peter Febriano Kapitan, Maxs Urias Ebenhaezer Sanam, Elisabet Tangkonda, Fhady Risckhy Loe
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
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