Gambaran Anatomi dan Histologi Testis Ayam Hutan Hijau (Gallus varius) Asal Pulau Alor
Abstract
Green junglefowl (Gallus varius) is one of the endemic fowl in Indonesia. Testes are reproductive organs that play an important role in spermatogenesis. This study aims to describe the anatomical morphology and histology of the green junglefowl testes. The sample is 3 pairs of testes collected from 3 adult male green junglefowl from Alor Island with body weight range about 600 grams to 800 grams and age range about 1 to 2 years. The sample were observed macroscopically including the location, shape, color, consistency, weight, length and width. Furthermore, the sample were fixed with 10% formalin solution and continued with the process of making preparations and staining with hematoxylin-eosin (HE). The results showed that the testes of the green junglefowl were located in the abdominal cavity which was seen by the mesorchium. The testes are adjacent to several organs such as kidney, liver, spleen and proventriculus. The testes are asymmetrical, that is the left testicle is longer than the right testicle, while the width of the testicle is between 1.1 mm to 3.4 mm. The testicular weight is <1 gram. The testes are light yellow in color, have a smooth surface and are soft in consistency. Histologically, the testes of jungle fowl are composed of seminiferous tubules and interstitial tissue, and surrounded by a testicular capsule consisting of layers of tunica serous, tunica albuginea and tunica vasculosa. Sertoli cells and spermatogenic cells consisting of spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids are scattered in the seminiferous tubules. The interstitial testes contain several components such as connective tissue, Leydig cells and blood vessels.
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References
Aire TA, Ozegbe PC. 2007. The testicular capsule and peritubular tissue of birds: morphometry, histology, ultrastructure and immunohistochemistry. Journal of Anatomy, 210(6): 731-740.
Al-Tememy HSA. 2010. Histological study of testis in Quail (Coturnix coturnix japonica). Al-Anbar Journal of Veterinary Science, 3(2): 36-44.
Bahmid NA. 2015. Studi morfologi dan histomorfometrik testis ayam ketawa usia 1 bulan sampai 4 bulan [Skripsi]. Makassar: Program Studi Kedokteran Hewan, Fakultas Kedokteran, Universitas Hasanuddin.
Bakst MR, Akuffo V, Trefil P, Brillard JP. 2007. Morphological and histochemical characterization of the seminiferous epithelial and Leydig cells of the turkey. Animal Reproduction Science, 97(3-4):303-313.
[BLI] BirdLife International. 2021. Spesies factsheet: Gallus varius. The IUCN Red List for Bird. http://www.birdlife.org [4 Maret 2021].
Bull ML, Martins MRFB, Cesario MD, Padovani CR, Mendes AA. 2007. Anatomical study on domestical fowl (Gallus domesticus) reproductive system. International Journal of Morphology, 25(4): 709-716.
Calhim S, Montgomerie R. 2014. Testis asymmetry in birds: the influences of sexual and natural selection. Journal of Avian Biplogy, 46(2): 1-11.
Castillo A, Margherita M, Andrea P, Isabella R. 2012. Histological observations in testes of hybrids of Gallus gallus x Phasianuns colchicus. Avian Biology Research, 5(1): 1-10.
Dharani P, UshaKumary S, Venkatesan S, Cecilia Joseph, Ramesh G. 2017. Morphometry and histology of the testicular capsule and peritubular tissue of testis of guinea fowl (Numida maleagris). Indian Journal of Veterinary Anatomy, 29(1): 67-69.
Elbajory SIA, Tingari MDE, Abdalla MA. 2013. Morphological study of the testis of adult Sudanese duck (Anas platyrhynchos). International Journal Animal and Veterinary Advances, 5(3): 103-107.
Eurell JA, Frappier BL (ed.). 2006. Dellmann’s Textbook of Veterinary Histology, 6th edition. USA: Blackwell Publishing.
Firwan NNZ, Akmal M, Masyitha D, Salim MN, Jalaluddin M, Siregar TN. 2020. Histology and Hhstomorphometry of testes in turkeys (Meleagris gallopavo) Based on Age Level. Advances in Biological Sciences Research, 12(2): 215-220.
Freneau GE, Carvalho SFM, Morais SMTS, Freneau BN. 2016. Aspects of spermatogenesis and microscopic testicular morphology in greater rhea, Rhea americana (Linnaeus, 1758). Pesquisa Veterinária Brasileira, 36(10): 1045-1052.
Hassan SA, Moussa EA. 2012. Gross and microscopic studies on stomach of domestic duck (Anas platyrhynchos) and domestic pigeon (Columba livia domestica). Journal of Veterinary Anatomy, 5(2): 105-127.
Kareem DA, Jassem ES, Daaj SA, Al-Khalad WJ. 2020. Morphological and histological study of the testes in adult duck. Plant Archives, 20(2): 751-755.
Khatun P, Haque Z, Das SK. 2019. Histology of the male gonad of adult Khaki Campbell duck (Anas platyrhynchos domesticus) in Bangladesh. International Journal of Veterinary Sciences and Animal Husbandry, 4(4): 36-39.
Konig HE, Korbel R, Liebich HG (ed). 2016. Avian Anatomy Textbook and Colour Atlas. Translated from Germany: Corinna K. UK: 5M Publishing Ltd.
Lake PE. 1957. The male reproductive tract ot the fowl. Journal of Anatomy, 91(1):116-29.
Lawal RA, Martin SH, Vanmenchelen K, Vereijken A, Silva P, Al-Atiyat RM, et al. 2020. The wild species genome ancestry of domestic chickens. BMC Biology, 18(13): 1-18.
Mansjoer SS. 1987. Habitat dan performans ayam hutan di Indonesia. Media peternakan, 12: 1-7.
Ozegbe PC, Aire TA, Madekurozwa M-C, Soley JT. 2008. Morphological and immunohistochemical study of testicular capsule and peritubular tissue of emu (Dromaius novaehollandiae) and ostrich (Struthio camelus). Cell and Tissue Research, 332(1):151-158..
Pollock CG, Orosz SE. 2002. Avian reproductive anatomy, physiology and endocrinology. The Veterinary Clinics Exotic Animal Practice, 5(3): 441-474.
Saleem R, Singh B, Mohd KI, Singh I, Bharti SK. 2017. Gross and biometrical studies on male reproductive system of adult local fowl of Uttarakhand (Uttara fowl). International Journal of Pure and Applied Bioscience, 5(3): 634-638.
Sawai H, Kim HL, Kuno K, Suzuki S, Gotoh H, Takada M, et al. 2010. The origin and genetic variation of domestic chickens with special reference to junglefowls Gallus g. gallus and G. varius. PloS one, 5(5): 1-11.
Scanes CG (ed). 2015. Sturie’s Avian Physiology, 6th edition. USA: Elsevier Inc.
Sibley CG, Monroe BL. 1990. Distribution and Taxonomy of Birds of the World. New Haven & London: Yale University Press.
Sotiyono. 2001. Pengenalan Organ Reproduksi Ayam. Semarang: PT. Perhutani Persero.
Sturkie PD (Ed). 1986. Avian Physiology, 4th edition. New York: Springer-Verlag.
Tamilselvan S, Dhote BS, Ishwar S, Meena MS, Sathapathy, Mahanta D. 2018. Gross morphology of testes and gonadosomatic index (GSI) of guinea fowl (Numida meleagris). Journal of Entomology and Zoology Studies, 6(3): 156-159.
Zein MSA, Sulandari S. 2009. Investigasi asal usul ayam Indonesia menggunakan sekuens hypervariable-1 D-loop DNA mitokondria. Jurnal Veteriner, 10(1):41-49.
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