Kualitas Spermatozoa Babi dalam Pengencer Air Buah Lontar dan Kuning `Telur Ayam Kampung dengan Metode Penyimpanan yang Berbeda
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Maria Selviana Bebbe Bei(1*)
Universitas Nusa Cendana
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Nancy D F K Foeh(2)
Universitas Nusa Cendana
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Cynthia Dewi Gaina(3)
Universitas Nusa Cendana
(*) Corresponding Author
Keywords:
yolk, water jacket, lontar
Abstract
The aim of this study is to find the best concentration ratio of native chicken egg yolk (NCEY) and lontar water (LW) in order to preserve landrace boar semen, with water jacket and non-water jacket method, at 5°C and to find how long can it preserve the semen. Fresh semen was collected from Landrace boar 2-4 years old age. Semen sample is categorized apropriate to be extended if >70% sperm motility, >200x106 cells/ mL, and >20% sperm abnormality. This study was designed with compeltely ranomized design and faktorial patern with 2 controled group, 6 treated group, and 4 repitition. Controled group consist of fresh semen without extender. K0 was preserved with non-water jacket method, and K1 was preserved with water jacket method. Controled group were P1 (95% LW + 5% NCEY), P2 (85% LW + 15% NCEY), P3 (75% LW + 25% NCEY) that preserved with water jacket method, and P4 (95% LW + 5% NCEY), P5 (85% LW + 15% NCEY), P6 (75% LW + 25% NCEY) preserved with non-water jacket method. Sperm motility and viablity were evaluated every 2 hours until motility value has reached 40%. The result shows that P2 is the best concentration to preserve sperm motility (26 hours, 45.00±0.00%) and viability (30 hours, 51.00±0.40%).
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References
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Colenbrander, B., Fazeli, A.R., Van Buiten, A., Parlevliet, J.,Gadella, B.M. 1992, Assesment of Sperm Cell Membran Integrity in the Horse. Acta Veterinaria Scandinavica Journal, 88:49-58.
Daramola, J.O., Adekunale, E.O., Oke, O.E., Onagbesan, I.K., Oyewusi, I.K. and Oyewusi, J.A. 2016, Effects of Coconut (Cocos nucifera) Water with or without Egg-Yolk on Viability of Criofpreserved Buck Spermatozoa, Animal Reproduction Journal, 13(2):57-62.
Dapawole, R.R. 2014, Preservasi dan Kriopreservasi Semen Babi dalam Pengencer BTS dan MIII yang disuplementasi dangan dan tanpa Trehalosa, Tesis, Institut Pertanian Bogor, Bogor.
Delviona, F.Y. 2016,’Kualitas Semen Cair Babi dalam Pengencer Beltsville Thawing Solution yang Ditambah Kuning Telur dan Kuning Telur dan Gliserol Disimpan pada Suhu 5 oC dan 18 oC, Skripsi, Institut Pertanian Bogor, Bogor.
Ducha, N., Susilawati, T., Aulanni’am, dan Wahyuningsih, S. 2013, Motilitas dan Viabilitas Spermatozoa Sapi Limousin selama Penyimpanan pada Refrigerator dalam Pengencer CEP-2 dengan Suplementasi Kuning Telur, Jurnal Kedokteran Hewan, 7(1):5-8.
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Frunza, I., Cernescu, H., Korodi, G. 2008, Physical and Chemical Parameters of Boar Semen, Lucrari Stiintifice Medicina Veterinara, 41:634-640.
Garner, D.L., Hafez, E.S.E. 2000, Spermatozoa and Seminal Plasma. In: Hafez, B. and Hafez, E.S.E., editor, Reproduction in Farm Animal, 7th Ed, Lippincott Williams and Wilkins, Philadelpia, p 365-389.
Gadea, J. 2003, Semen Extenders Used in the Artificial Insemination of Swine, Spanish Journal of Agricultural Research, 1(2):17–27.
Johnson, L.A., Weitze, K.F., Fisher, P., and Maxwell, W.M.C. 2000, Storage of Boar Semen, Animal Reprodction Science Journal, 62:143-172.
Kaka, A., Nalley, W.M., Kune, P. dan Burhanuddin, 2014, Persentase Nira Lontar (Borassus flabellifer L) dalam Pengencer Tris–Kuning Telur terhadap Kualitas Semen Cair Kambing Peranakan Etawah yang disimpan pada Suhu 3-5 ºC, Jurnal Nukleus Petrenakan, 1(1):21-27.
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Kommisrud, E., Paulenz, H., Sehested, E., Grevle, I.S. 2002, Influence of Boar and Semen Parameters on Motility and Acrosome Integrity in Liquid Stored for Five Days, Acta Veterinaria Scandinavica Journal, 43:49-51.
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Manjunath, P. 2012, New Insights into the Understanding of the Mechanism of Sperm Protection by Extender Components, Animal Reproduction Journals. 9(4):809-815.
Matahine, T., Burhanuddin Dan Marawali A. 2014, Efektivitas Air Buah Lontar dalam Mempertahankan Motilitas, Viabilitas dan Daya Tahan Hidup Spermatozoa Sapi Bali, Jurnal Veteriner, 15(2):263-273.
Nalley, M., Gaina, C.D., Belli, H.L.L. 2015, Penuntun Praktikum Ilmu dan Teknologi Reproduksi, Fakultas Kedokteran Hewan, Universitas Nusa Cendana, Kupang.
Neno, M.E.W., 2016, ‘Pengaruh Pengencer Komersial dengan Metode Water Jacket dan Non Water Jacket terhadap Kualitas Semen Babi Landrace’, Skripsi, Fakultas Kedokteran Hewan, Universitas Nusa Cendana, Kupang.
Paulenz, H., Kommisrud, E. and Hofmo, P.O. 2000, Effect of Long-Term Storage at different Tempertures on the Quality of Liquid Boar Semen. Reproduction in Domestic Animals Journal. 35:83–85.
Prastowo, A. 2008,’ Morfologi dan Morfometri Spermatozoa Babi Yorkshire dalam Nilai Ejakulat dengan Pewarnaan Wiliams’, Skripsi, Institut Pertanian Bogor, Bogor.
Rizal, M. dan Herdis. 2008, Inseminasi Buatan pada Domba, Rineka Cipta, Jakarta.
Rodriguez, A. L. 2012, ‘Boar Semen : Quality Control and Production’, Disertation, Ghent University, Belgia.
Shanon, P. and Curson, R. 1971, Toxic Effect and Action of Dead Sperm on Diluted Bovine Semen, Journal of Dairy Scence, 55(5):614-620.
Soler, A.J., Guzman, M.D., dan Garde, J.J. 2003, Storage of Red Deer Epididymides for Four Days at 5 oC : Effect on Sperm Motility, Viability and Morphology Integrity, Journal of Experimental Zoology, 29A(2):188-199.
Standar Nasional Indonesia. 2014. Semen cair babi. Badan Standarisasi Nasional.
Sumardani, N.L.G. 2007,‘Viabilitas dan Fertilitas Spermatozoa dalam Modifikasi Pengencer BTS Dan Zorlesco dengan Penyimpanan Berbeda dalam Rangkaian Inseminasi Buatan pada Babi’ Tesis, Institut Pertanian Bogor, Bogor.
Sumardani, N.L., Tuty, L.Y. dan Siagian, P.H. 2008, Viabilitas Spermatozoa Babi dalam Pengencer BTS (Bestville Thawing Sollution) yang Dimodifikasi pada Penyimpanan Berbeda, Jurnal Media Peternakan, 31(2):81-86.
Susilawati, T. 2011, Spermatology, Universitas Brawijaya Press, Malang.
Susilorini, E.T. 2008, Budidaya 22 Ternak Potensial, Penebar Swadaya, Jakarta
Tamoes, J.A., Nalley, W.M dan Hine, T.M. 2014, Fertilitas Spermatozoa Babi Landrace dalam Pengencer Modifikasi Zorlesco dengan Susu Kacang Kedelai, Jurnal Sains Peternakan, 20(1):20-30.
Toelihere, M.R. 1993, Inseminasi Buatan pada Ternak. Angkasa, Bandung.
Triharyanto, B. 2001, Peternakan Ayam Arab, Kanisius, Yogyakarta.
Vengaiah, P.C., Vijaya, K.B., Murti, G.N. and Prasad, K.R. 2015. Physico-Chemical Propertiesof Palmyrah Fruit Pulp (Borassus flabellifer L.), Journal of Nutrition and Food Sciences, 5(5):1-4.
Vyt, P. 2007, ‘Examination and Storage of Liquid Porcine Semen’, Thesis, Ghent University, Belgia.
Watson, P. F. 1996, Cooling of Spermatozoa and Fertilizing Capacity, Reproduction in Domestic Animals Journal, 31:135-140.
Wilson, M.E.,Rozeboom, K.J. and Crenchaw, T.D. 2004, Boar Nutrition for Optimum Sperm Production, Advances in Pork Production, 15:295-306.
Yusuf, T.L., Arifiantini, R.I. dan Mulyadi Y. 2006, Efektifitas Waktu Pemaparan Glicerol terhadap Motilitas Spermatozoa pada Pembekuan Semen Domba Lokal menggunakan Pengencer Tris Kuning Telur, Animal Production Journal, 8(3):168-173.
Cardoso, R.C., Silva, A.R., Uchoa,D.C., da Silva, L.D. 2003, Cryopreservation of Canine Semen using a Coconut Water Extender with Egg Yolk and Three different Glycerol Concentrations, Theriogenology Journal, 59(3):743-751.
Colenbrander, B., Fazeli, A.R., Van Buiten, A., Parlevliet, J.,Gadella, B.M. 1992, Assesment of Sperm Cell Membran Integrity in the Horse. Acta Veterinaria Scandinavica Journal, 88:49-58.
Daramola, J.O., Adekunale, E.O., Oke, O.E., Onagbesan, I.K., Oyewusi, I.K. and Oyewusi, J.A. 2016, Effects of Coconut (Cocos nucifera) Water with or without Egg-Yolk on Viability of Criofpreserved Buck Spermatozoa, Animal Reproduction Journal, 13(2):57-62.
Dapawole, R.R. 2014, Preservasi dan Kriopreservasi Semen Babi dalam Pengencer BTS dan MIII yang disuplementasi dangan dan tanpa Trehalosa, Tesis, Institut Pertanian Bogor, Bogor.
Delviona, F.Y. 2016,’Kualitas Semen Cair Babi dalam Pengencer Beltsville Thawing Solution yang Ditambah Kuning Telur dan Kuning Telur dan Gliserol Disimpan pada Suhu 5 oC dan 18 oC, Skripsi, Institut Pertanian Bogor, Bogor.
Ducha, N., Susilawati, T., Aulanni’am, dan Wahyuningsih, S. 2013, Motilitas dan Viabilitas Spermatozoa Sapi Limousin selama Penyimpanan pada Refrigerator dalam Pengencer CEP-2 dengan Suplementasi Kuning Telur, Jurnal Kedokteran Hewan, 7(1):5-8.
Foeh, N.D.F.K. dan Gaina, C.D. 2017, Sari Buah Lontar sebagai Pengencer Alami dalam Mempertahankan Kualitas Spermatozoa Babi, Jurnal Kajian Veteriner, 5(1):52-58.
Frunza, I., Cernescu, H., Korodi, G. 2008, Physical and Chemical Parameters of Boar Semen, Lucrari Stiintifice Medicina Veterinara, 41:634-640.
Garner, D.L., Hafez, E.S.E. 2000, Spermatozoa and Seminal Plasma. In: Hafez, B. and Hafez, E.S.E., editor, Reproduction in Farm Animal, 7th Ed, Lippincott Williams and Wilkins, Philadelpia, p 365-389.
Gadea, J. 2003, Semen Extenders Used in the Artificial Insemination of Swine, Spanish Journal of Agricultural Research, 1(2):17–27.
Johnson, L.A., Weitze, K.F., Fisher, P., and Maxwell, W.M.C. 2000, Storage of Boar Semen, Animal Reprodction Science Journal, 62:143-172.
Kaka, A., Nalley, W.M., Kune, P. dan Burhanuddin, 2014, Persentase Nira Lontar (Borassus flabellifer L) dalam Pengencer Tris–Kuning Telur terhadap Kualitas Semen Cair Kambing Peranakan Etawah yang disimpan pada Suhu 3-5 ºC, Jurnal Nukleus Petrenakan, 1(1):21-27.
Knox, R.V. 2006, ‘Semen Processing, Extending & Storage for Artificial Insemination in Swine’, Departemen of Animal Science, University of Illinois.
Kommisrud, E., Paulenz, H., Sehested, E., Grevle, I.S. 2002, Influence of Boar and Semen Parameters on Motility and Acrosome Integrity in Liquid Stored for Five Days, Acta Veterinaria Scandinavica Journal, 43:49-51.
Kostama, T. dan Sutama, I.K. 2006, Studi Motilitas dan Daya Hidup Spermatozoa Kambing Boer pada Pengencer Tris Sitrat-Fruktosa, Jurnal Sain Vet, 24(1): 58-64.
Manjunath, P. 2012, New Insights into the Understanding of the Mechanism of Sperm Protection by Extender Components, Animal Reproduction Journals. 9(4):809-815.
Matahine, T., Burhanuddin Dan Marawali A. 2014, Efektivitas Air Buah Lontar dalam Mempertahankan Motilitas, Viabilitas dan Daya Tahan Hidup Spermatozoa Sapi Bali, Jurnal Veteriner, 15(2):263-273.
Nalley, M., Gaina, C.D., Belli, H.L.L. 2015, Penuntun Praktikum Ilmu dan Teknologi Reproduksi, Fakultas Kedokteran Hewan, Universitas Nusa Cendana, Kupang.
Neno, M.E.W., 2016, ‘Pengaruh Pengencer Komersial dengan Metode Water Jacket dan Non Water Jacket terhadap Kualitas Semen Babi Landrace’, Skripsi, Fakultas Kedokteran Hewan, Universitas Nusa Cendana, Kupang.
Paulenz, H., Kommisrud, E. and Hofmo, P.O. 2000, Effect of Long-Term Storage at different Tempertures on the Quality of Liquid Boar Semen. Reproduction in Domestic Animals Journal. 35:83–85.
Prastowo, A. 2008,’ Morfologi dan Morfometri Spermatozoa Babi Yorkshire dalam Nilai Ejakulat dengan Pewarnaan Wiliams’, Skripsi, Institut Pertanian Bogor, Bogor.
Rizal, M. dan Herdis. 2008, Inseminasi Buatan pada Domba, Rineka Cipta, Jakarta.
Rodriguez, A. L. 2012, ‘Boar Semen : Quality Control and Production’, Disertation, Ghent University, Belgia.
Shanon, P. and Curson, R. 1971, Toxic Effect and Action of Dead Sperm on Diluted Bovine Semen, Journal of Dairy Scence, 55(5):614-620.
Soler, A.J., Guzman, M.D., dan Garde, J.J. 2003, Storage of Red Deer Epididymides for Four Days at 5 oC : Effect on Sperm Motility, Viability and Morphology Integrity, Journal of Experimental Zoology, 29A(2):188-199.
Standar Nasional Indonesia. 2014. Semen cair babi. Badan Standarisasi Nasional.
Sumardani, N.L.G. 2007,‘Viabilitas dan Fertilitas Spermatozoa dalam Modifikasi Pengencer BTS Dan Zorlesco dengan Penyimpanan Berbeda dalam Rangkaian Inseminasi Buatan pada Babi’ Tesis, Institut Pertanian Bogor, Bogor.
Sumardani, N.L., Tuty, L.Y. dan Siagian, P.H. 2008, Viabilitas Spermatozoa Babi dalam Pengencer BTS (Bestville Thawing Sollution) yang Dimodifikasi pada Penyimpanan Berbeda, Jurnal Media Peternakan, 31(2):81-86.
Susilawati, T. 2011, Spermatology, Universitas Brawijaya Press, Malang.
Susilorini, E.T. 2008, Budidaya 22 Ternak Potensial, Penebar Swadaya, Jakarta
Tamoes, J.A., Nalley, W.M dan Hine, T.M. 2014, Fertilitas Spermatozoa Babi Landrace dalam Pengencer Modifikasi Zorlesco dengan Susu Kacang Kedelai, Jurnal Sains Peternakan, 20(1):20-30.
Toelihere, M.R. 1993, Inseminasi Buatan pada Ternak. Angkasa, Bandung.
Triharyanto, B. 2001, Peternakan Ayam Arab, Kanisius, Yogyakarta.
Vengaiah, P.C., Vijaya, K.B., Murti, G.N. and Prasad, K.R. 2015. Physico-Chemical Propertiesof Palmyrah Fruit Pulp (Borassus flabellifer L.), Journal of Nutrition and Food Sciences, 5(5):1-4.
Vyt, P. 2007, ‘Examination and Storage of Liquid Porcine Semen’, Thesis, Ghent University, Belgia.
Watson, P. F. 1996, Cooling of Spermatozoa and Fertilizing Capacity, Reproduction in Domestic Animals Journal, 31:135-140.
Wilson, M.E.,Rozeboom, K.J. and Crenchaw, T.D. 2004, Boar Nutrition for Optimum Sperm Production, Advances in Pork Production, 15:295-306.
Yusuf, T.L., Arifiantini, R.I. dan Mulyadi Y. 2006, Efektifitas Waktu Pemaparan Glicerol terhadap Motilitas Spermatozoa pada Pembekuan Semen Domba Lokal menggunakan Pengencer Tris Kuning Telur, Animal Production Journal, 8(3):168-173.
Published
2021-02-19
How to Cite
Bei, M., Foeh, N., & Gaina, C. (2021). Kualitas Spermatozoa Babi dalam Pengencer Air Buah Lontar dan Kuning `Telur Ayam Kampung dengan Metode Penyimpanan yang Berbeda. Jurnal Veteriner Nusantara, 4(1), 12. https://doi.org/10.35508/jvn.v4i1.6046
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Articles
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