PENGARUH WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM TERMODIFIKASI (Effect of equilibration time on the quality of landrace boar frozen semen in modified durasperm extender)

  • Sofia Marlize Universitas Nusa Cendana
  • Thomas M. Hine Universitas Nusa Cendana
  • Wilmientje M. Nalley Universitas Nusa Cendana
Keywords: Equilibration time, frozen semen quality, landrace boar, modified durasperm

Abstract

This study aims to determine the effect of equilibration time on the quality of landrace spermatozoa both pre- and post-freezing and to find the best equilibration time. The materials used is fresh semen collected from a 2,5 years old landrace boar. Collecting was carried out twice per week using the glove hand method. Semen of good quality (sperm motility ≥70%, sperm concentration ≥200 x 106 cells/ml, and percentage of sperm abnormalities ≤15%) was diluted with durasperm that had been modified with lontar fruit juice, while the cryoprotectants used glycerol and sucrose. Holding time was carried out for 2 hour at 27-28°C, centrifuged at 2000 rpm for 15 minutes, and the sperm sediment was diluted with the same diluent. After that, the liquid semen was packed in a 0.5 mL straw and equilibrated at 3-5°C for 1 (P1), 2 (P2), or 3 hours (P3). The freezing of semen was carried out on the surface of liquid nitrogen at a distance of 5 cm for 10 minutes, and then stored in a liquid nitrogen container. Thawing is done by placing the frozen semen straws in warm water (37°C) for 30 seconds. The research data were analyzed with analysis of variance and continued with the Duncan test. The results showed that the equilibration time at 2 hours (P2) gave the best results (P<0,05) with the mean percentage of motility, viability, abnormality, intact plasma membrane and recovery rate respectively were 30,62%, 58,21%, 4,68%, 58,94% and 43,74%. It was concluded that the equilibration time did not affect the quality of pre-freezing spermatozoa, but it did affect the quality of post-freezing spermatozoa with the best equilibration time to maintain the quality of frozen semen of landrace boar was 2 hours.

References

Afiati F. Yulnawati, Riyadi M, Arifiantini RI. 2015. Abnormalitas spermatozoa domba dengan frekuensi penampungan berbeda. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia 1(4): 930-934.
Aku AS, Sandiah N, Sadsoeitoeboen PD, Amin MR, Herdis. 2007. Manfaat Lesitin Nabati pada Preservasi dan Kriopreservasi Semen: Suatu Kajian Pustaka. Animal Production 9(1): 49-52.
Ardana I, Putra D. 2008. Ternak Babi (Manajemen Reproduksi, Produksi Dan Penyakit). Udayana University Perss.
Arifiantini R. 2012. Teknik Koleksi Dan Evaluasi Semen Pada Hewan. PT. Penerbit IPB Press.
Arifiantini R, Ferdian F. 2006. Tinjauan aspek morfologi dan morfometri spermatozoa kerbau rawa (Bubalus bubalis) yang dikoleksi dengan tekni masase. Jurnal Veteriner 7(2): 83-91.
Ax R, Dally M, Didion B, Lenz R, Love C, Varner D, Hafez B, Bellin M. 2000. Semen Evaluation. (B. Hafez & E. Hafez (eds.); 7th ed.). Lippincott Williams and Wilkins.
Bag S, Joshi A, Rawat P, Mittal J. 2001. Effect of Initial freezing temperature on the semen characteristics of frozen-thawed ram spermatozoa in a semi-arid tropical environment. Small Ruminant Research 43: 23-29.
Bearden H, Fuquay J, Willard S. 2004. Applied Animal Reproduction (6th ed.). Pearson Prentice Hall.
Bebas W, Gorda W. 2020. Kadar Krioprotektan gliserol dan dimethylsulfoxide terbaik pada pengencer astaxanthin fosfat kuning telur bebek terhadap kualitas semen beku babi. Jurnal Veteriner 21(1): 115-123.
Best B. 2006. Viability, Cryoprotectants toxicity and chilling injury in cryonics. https://www.benbest.com/cryonics/viable. html.
Febriani G, Hamdan, Melia J. 2014. Pengaruh waktu ekuilibrasi terhadap kualitas semen kerbau lumpur (Bubalus bubalis) setelah thawing. Jurnal Medika Veterinaria 8(1): 1-3.
Feka W, Dethan A, Beyleto V. 2016. Pengaruh lama waktu penyimpanan terhadap viabilitas dan pH semen babi landrace yang diencerkan menggunakan bahan pengencer sitrat kuning telur. Journal of Animal Science 1(3): 34-35.
Feradis M. 2010. Bioteknologi Reproduksi pada Ternak (1st ed.). Alfabeta.
Foeh NDFK. 2015. Kualitas semen beku babi dalam pengencer BTS dan MIII menggunakan krioprotektan dimethyl-acetamide dan gliserol dengan sodium dedocyl sulphate. Institut Pertanian Bogor.
Foeh NDFK, Arifiantini RI, Yusuf TL. 2017. The quality of boar frozen semen diluted in bts® and mii® with different cryoprotectant supplemented with sodium dodecyl sulphate. Jurnal Kedokteran Hewan 11(1): 6-10.
Garner DL, Hafez ESE. 2000. Spermatozoa and seminal plasma. Reproduction in Farm Animals, 96-109.
Herdis, Rizal M. 2008. Inseminasi Buatan pada Domba. Rineka Cipta.
Johnson LA, Weitze KF, Fiser P, Maxwell WMC. 2000. Storage of boar semen. Animal Reproduction Science 62(1-3): 143-172.
Kaka A. 2020. Karakteristik dan daya fertilitas spermatozoa babi peranakan landrace. Jurnal Peternakan Indonesia 22(3): 277-283.
Knox R. 2006. Semen Processing, Extending & Storage For Artificial Insemination In Swine. Departement of Animal Sciences University of Illinois Publications, 1-7.
Kostaman T, Sutama IK. 2006. Studi motilitas dan daya hidup spermatozoa kambing boer pada pengencer tris-sitrat-fruktosa. Jurnal Sains Veteriner 1(24): 58-64.
Naing SW, Wahid H, Azam KM, Rosnina Y, Zuki AB, Kazhal S, Bukar MM, Thein M, Kyaw T, San MM. 2010. Effect of sugars on characteristics of Boer goat semen after cryopreservation. Animal Reproduction Science 122(1-2): 23-28.
Nalley WM, Handarini R, Yusuf TL, Purwantara B, Semiadi G. 2011. The effect of glycerol concentration in Tris glucose egg yolk extender on the quality of Timor Deer frozen semen. Journal of the Indonesian Tropical Animal Agriculture 36(2): 91-96.
Pangestu M. 2002. Preservation of spermatozoa: methods and applications. indonesian forum on reproduction. Journal on Reproduction 1(2): 55-56.
Parker J. 2000. Reproductive Physiology In Poultry (E. Hafez (ed.)). Lippincott Williams & Wilkins.
Salamon S, Maxwell W. 2000. Storage of ram semen. Animal Reproduction Science 62(1-3): 77-111.
Sangma TFM, Kutubuddin A, Mitali DC, Galib UZ, Nekibuddin A, Arunoday D. 2020. Characteristics of fresh crossbred Hampshire boar semen. Haryana Veterinarian 59(1): 98-101.
Subrata I, Artiningsih N, Sumardani N, Putra W, Umiarti A. 2014. Pengaruh bahan pengencer biologis terhadap kualitas semen babi Hampshire. Prosiding. Fakultas Peternakan Universitas Udayana.
Suherlan NE, Soeparna, Hidajat K. 2015. Pengaruh penambahan berbagai tingkat DMF (dimethylformamide) sebagai agen krioprotektan terhadap keutuhan membran plasma dan recovery rate semen beku domba lokal. Jurnal Ilmu-Ilmu Peternakan 4(4): 1-12.
Sumardani N. 2007. Viabilitas dan fertilitas spermatozoa dalam modifikasi pengencer BTS dan zorlesco dengan penyimpanan berbeda dalam rangkaian inseminasi buatan pada babi. Thesis. Institut Pertanian Bogor.
Sumardani N, Budaarsa K, Putri TI, Puger AW. 2019. Umur memengaruhi volume semen dan motilitas spermatozoa babi landrace di Balai Inseminasi Buatan Baturiti, Tambanan, Bali. Jurnal Veteriner 20(3): 324-329.
Sumardani N, Tuty L, Siagian P. 2008. Viabilitas spermatozoa babi dalam pengencer BTS (Beltsville Thawing Solution) yang dimodifikasi pada penyimpanan berbeda. Media Peternakan 31(2): 81-86.
Surachman M, Herdis, Yulnawati, Rizal M, Maheshwari H. 2009. Kualitas semen cair asal epididimis kerbau belang dalam bahan pengencer andromed yang mendapat penambahan sukrosa. Media Peternakan 32(2): 88-94.
Umar S, Maharani M. 2005. Pengaruh berbagai waktu ekuilibrasi terhadap daya tahan sperma sapi limousin dan uji kebuntingan. Jurnal Agribisnis Peternakan 1(1): 17-21.
Yusuf T, Arifiantini R, Dapawole R, Nalley WM. 2017. Kualitas semen beku babi dalam pengencer komersial yang disuplementasi dengan trehalosa. Jurnal Veteriner 18(1): 69-75.
Published
2021-12-14
How to Cite
Marlize, S., Hine, T., & Nalley, W. (2021). PENGARUH WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM TERMODIFIKASI (Effect of equilibration time on the quality of landrace boar frozen semen in modified durasperm extender). JURNAL NUKLEUS PETERNAKAN, 8(2), 150-160. https://doi.org/10.35508/nukleus.v8i2.4867
Section
Articles

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.