Effect of Garlic (Allium sativum) Extract Administration on the Histopathological Features of Mammary Glands in Female Sprague-Dawley Rats Induced with 7,12-Dimethylbenz(a)anthracene (DMBA)
Pengaruh Pemberian Ekstrak Bawang Putih (Allium sativum) terhadap Gambaran Histopatologi Payudara Tikus Putih Sprague-Dawley Diinduksi 7,12 Dimetilbenz(A)Antrasen (DMBA)
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Intan Putri Dewanti(1*)
Universitas Nusa Cendana
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Efrisca Meliyuta Br Damanik(2)
-
Anita Lidesna Shinta Amat(3)
-
I Nyoman Sasputra(4)
(*) Corresponding Author
Keywords:
Kanker Payudara, Allium sativum, DMBA, epithel duktus, Sprague-Dawley
Abstract
Latar Belakang: Kanker adalah penyebab utama kedua kematian secara global, terhitung sekitar 9,6 juta kematian, atau satu dari enam kematian pada tahun 2018. Angka insidensi kanker payudara sebanyak 11.6% dan angka kematian 6,6% pada tahun 2018. Tanaman obat diduga memiliki potensi sebagai antikanker dengan efek samping minimal jika digunakan dengan dosis dan waktu penggunaan yang tepat serta cara penggunaan yang tepat.
Tujuan Penelitian : Untuk mengetahui pengaruh pemberian ektrak bawang putih terhadap gambaran histopatologi payudara tikus Sprague-Dawley betina yang diinduksi DMBA.
Metodologi : Penelitian ini menggunakan metode eksperimental post test only control group design dengan 6 kelompok perlakuan. Sampel sebanyak 30 tikus setiap kelompok berisi 5 ekor tikus. Data dianalis dengan menggunakan uji normalitas Shapiro-wilk dan uji non-parametrik Kruskal Wallis.
Hasil : Hasil penelitian didapatkan tidak terdapat pengaruh pada pemberian ekstrak bawang putih terhadap gambaran histopatologi payudara tikus Sprague-Dawley betina yang diinduksi DMBA. Kelompok 1 epithel duktus normal. Kelompok 2 perubahan epithel duktus hiperplasia dengan atipia. Kelompok 3, kelompok 4, kelompok 5, kelompok 6 perubahan epithel duktus severe hiperplasia. Hasil uji statistik Kruskall-Wallis didapatkan perbedaaan yang tidak signifikan (p>0,05). Hasil uji post hoc Mann-Whitney pada kelompok 1 dan 2 didapatkan perbedaaan yang signifikan (p<0,05).
Kesimpulan : Pemberian ekstrak bawang putih (Allium sativum) tidak dapat memberikan perubahan terhadap gambaran histopatologi payudara tikus Sprague-Dawley betina yang diinduksi 7,12 Dimetilbenz(a)antrasen (DMBA).
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11. Kusnul Z, Suryono S, Tamsuri A. Ekstrak Propolis Memperbaiki Profil Berat Badan Tikus Model Kanker Payudara yang Diinduksi dengan 7,12-dymethyilbenz(a) antracene (DMBA). Media Penelit dan Pengemb Kesehat. 2019;29(2):135-142. doi:10.22435/mpk.v29i2.640
12. Kombinasi Doxorubicin dan Ekstrak Daun Pepaya E, Zuhrotun Nisa F, Astuti M, et al. Efek Kombinasi Doxorubicin dan Ekstrak Daun Pepaya (Carica papaya L.) terhadap Sel Payudara Pada Tikus Sprague Dawley yang Diinduksi DMBA (7,12-Dimethilbenzene(a)ntrazena). :71-76.
13. Ajami M, Vazirijavid R. Garlic (Allium Sativum L.). Elsevier Inc.; 2018. doi:10.1016/B978-0-12-812491-8.00033-3
14. Szychowski KA, Binduga UE, Rybczyńska-Tkaczyk K, Leja ML, Gmiński J. Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15. Saudi J Biol Sci. 2018;25(8):1703-1712. doi:10.1016/j.sjbs.2016.10.005
15. Antony S, Kumar KK, Menon JS. Assessment of phyto-constituents by GC-MS and anti-tumour activity of garlic grown in different altitude: A comparative study. Pharmacogn J. 2019;11(2):350-354. doi:10.5530/pj.2019.11.52
16. Biochemical Investigation of the Effect of Garlic (Allium sativum) on 7,12-dimethylbenz[a]anthracene (DMBA) Induced Mammary Cancer in Female Albino Rats. Asian J Biochem. 2006;1(3):251-256. doi:10.3923/ajb.2006.251.256
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18. Female Reproductive System. https://www.histology.leeds.ac.uk/female/FRS_breast.php. Accessed June 25, 2020.
19. What Are the Risk Factors for Breast Cancer? https://www.cdc.gov/cancer/breast/basic_info/risk_factors.htm.
20. Komite Penanggulangan Kanker Nasional. Panduan Penatalaksanaan Kanker Payudara. Kementeri Kesehat Republik Indones. 2015:1, 12-14, 24-26, 45. doi:10.1111/evo.12990
21. Breast Cancer Risk Factors You Cannot Change. https://www.cancer.org/cancer/breast-cancer/risk-and-prevention/breast-cancer-risk-factors-you-cannot-change.html.
22. Lifestyle related Breast Cancer Risk Factors. https://www.cancer.org/cancer/breast-cancer/risk-and-prevention/lifestyle-related-breast-cancer-risk-factors.html.
23. Bedah DI, Kedokteran F, Airlangga U. Gradasi Histopatologi sebagai predictor kejadian kekambuhan pada kanker paydara. 2016.
24. Fitriardi Sejati, Kiki Rizki RY. Hubungan Derajat Histopatologi DAN Invasi Limfovaskular Terhadap Metastasis Kelenjar Getah Bening Aksila Pada Kanker Payudara Dini (The Relationship Of Histopathological Degree And Lymphovascular Invasion On Metastasis Of Axilla Lymph Gland In Early B. 2019;3(1):37-49.
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27. Islam MS, Kusumoto Y, Al-Mamun MA. Cytotoxicity and Cancer (HeLa) Cell Killing Efficacy of Aqueous Garlic (Allium sativum) Extract. J Sci Res. 2011;3(2):375-382. doi:10.3329/jsr.v3i2.6557
28. Terhadap H, Massa I, Darah T, Darah DANG. Pengaruh Suplementasi Ekstrak Daun Annonamuricata Terhadap Kejadian Displasia Epitel Kelenjar Payudara Tikus Sprague Dawley yang Diinduksi 7, 1dimethylbenz[α]anthracene. 2011:1-22.
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30. Br DS. Pengaruh Ekstraksi Alliin Bawang Putih ( Allium Sativum L .) Terhadap Resiko Kanker Paru-Paru pada Mencit Percobaan yang Diinduksi Akrilamida. 2017.
31. Meiyanto E. Uji antikarsinogenesis. Prosedur CCRC (Cancer Chemoprevention Res Center) Fak Farm UGM. 2009.
32. Chhabria S V, Akbarsha MA, Li AP, Kharkar PS, Desai KB. In situ allicin generation using targeted alliinase delivery for inhibition of MIA PaCa-2 cells via epigenetic changes, oxidative stress and cyclin-dependent kinase inhibitor (CDKI) expression. Apoptosis. 2015;20(10):1388-1409. doi:10.1007/s10495-015-1159-4
33. Zhang X, Zhu Y, Duan W, Feng C, He X. Allicin induces apoptosis of the MGC-803 human gastric carcinoma cell line through the p38 mitogen-activated protein kinase/caspase-3 signaling pathway. Mol Med Rep. 2015;11(4):2755-2760. doi:10.3892/mmr.2014.3109
34. Rana S V., Pal R, Vaiphei K, Sharma SK, Ola RP. Garlic in health and disease. Nutr Res Rev. 2011;24(1):60-71. doi:10.1017/S0954422410000338
35. Singh V, Belloir C, Siess M-H, Le Bon A-M. Inhibition of Carcinogen-Induced DNA Damage in Rat Liver and Colon by Garlic Powders With Varying Alliin Content. Nutr Cancer. 2006;55(2):178-184. doi:10.1207/s15327914nc5502_9
36. Hewen, W., Nurina, R., & Liana, D. (2020). Pengaruh Pemberian Ektrak Bawang Putih (Allium Sativum) Terhadap Penurunan Kadar Kolesterol Total Tikus Putih (Rattus Norvegicus) Dengan Hiperkolesterol. Cendana Medical Journal (Cmj), 8(1), 572-580. Retrieved From Https://Ejurnal.Undana.Ac.Id/Cmj/Article/View/2668
Published
2025-07-16
How to Cite
Dewanti, I., Br Damanik, E., Amat, A., & Sasputra, I. N. (2025). Effect of Garlic (Allium sativum) Extract Administration on the Histopathological Features of Mammary Glands in Female Sprague-Dawley Rats Induced with 7,12-Dimethylbenz(a)anthracene (DMBA). Cendana Medical Journal, 12(2), 47-56. https://doi.org/10.35508/cmj.v12i2.10948
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