THE EFFECT OF RED BETEL LEAF ETHANOL EXTRACT (Piper crocatum)TO HISTOPATHOLOGICAL IMAGE OF RAT (Rattus norvegicus) CARDIAC MUSCLE INDUCED BY ALLOXAN
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Riky Alberth Lerrick(1*)
Universitas Nusa Cendana
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Efrisca Meliyuita Br. Damanik(2)
Universitas Nusa Cendana
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Anita Lidesna Shinta Amat(3)
Universitas Nusa Cendana
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Rr. Listyawati Nurina(4)
Universitas Nusa Cendana
(*) Corresponding Author
Abstract
Introduction: Red betel leaves (Piper crocatum) contain flavonoids, alkaloids, saponins, and tannins, which have antidiabetic properties. Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia due to impaired insulin secretion or action, leading to increased production of Reactive Oxygen Species (ROS). The antioxidants in red betel leaf extract are expected to reduce ROS formation and protect cardiac muscle from DM-induced damage..
Methods: This laboratory experimental study used a true experimental post-test only control group design. Thirty rats were divided into five groups: positive control (KK+), negative control (KK−), and treatment groups (KP1, KP2, KP3), each consisting of six rats. Histopathological features of cardiac muscle, including interstitial fibrosis, ventricular myocyte hypertrophy, and intramyocyte lipid deposits, were assessed. Data were analyzed using the Kruskal-Wallis test and Post Hoc LSD.
Results: There was a significant difference in ventricular myocyte hypertrophy between groups (p = 0.009), but no significant differences were observed in interstitial fibrosis (p = 1.000) and intramyocyte lipid deposits (p = 0.866).
Discussion: Red betel leaf extract at 150 mg/kg body weight effectively reduced ventricular myocyte hypertrophy in diabetic rats
Conclusion: Red betel leaf extract significantly reduced ventricular myocyte hypertrophy but had no effect on fibrosis or intramyocyte lipid deposits.
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References
2. Goyal, R., & Jialal, I. (2018). Diabetes Mellitus Type 2. Verdure: Health Science Journal.
3. International Diabetes Federation. (2021). IDF Diabetes Atlas 10th edition.
4. Kumar, Vinay. A. A. J. (2019). Buku Ajar Patologi Robbins - 10th Edition. Elsevier.
5. Perkeni. (2021) Pedoman Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 Dewasa di Indonesia.
6. Pusat Data dan Informasi Kementerian Kesehatan RI. (2020) Infodatin Diabetes Melitus.
7. Kemenkes (2018). Laporan Provinsi Nusa Tenggara Timur Riskesdas 2018.
8. Pickering, R. J., Rosado, C. J., Sharma, A., Buksh, S., Tate, M., & de Haan, J. B. (2018). Recent novel approaches to limit oxidative stress and inflammation in diabetic complications. In Clinical and Translational Immunology (Vol. 7, Issue 4). John Wiley and Sons Inc
9. Meng , J. M., Cao, S. Y., Wei, X. L., Gan, R. Y., Wang, Y. F., Cai, S. X., Xu, X. Y., Zhang, P. Z., & Li, H. bin. (2019). Effects and mechanisms of tea for the prevention and management of diabetes mellitus and diabetic complications: An updated review. In Antioxidants (Vol. 8, Issue 6). MDPI
10. Karwi, Q. G., Ho, K. L., Pherwani, S., Ketema, E. B., Sun, Q., & Lopaschuk, G. D. (2022). Concurrent diabetes and heart failure: interplay and novel therapeutic approaches. Cardiovascular Research
11. Parim, B., Sathibabu Uddandrao, V. v., & Saravanan, G. (2019). Diabetic cardiomyopathy: molecular mechanisms, detrimental effects of conventional treatment, and beneficial effects of natural therapy. In Heart Failure Reviews (Vol. 24, Issue 2, pp. 279–299).
12. Tan, Y., Zhang, Z., Zheng, C., Wintergerst, K. A., Keller, B. B., & Cai, L. (2020). Mechanisms of diabetic cardiomyopathy and potential therapeutic strategies: preclinical and clinical evidence. Nature Reviews Cardiology 2020
13. Patala, R., Utami, K., & Wahyuni, S. (2021). Potensi ekstrak daun sirih merah terhadap histopatologi pankreas tikus putih jantan yang diinduksi streptozotocin. Farmakologika jurnal farmasi, XVIII(2).
14. Hesti Dwi Handini, Rohmah, J., Laboratorium Medis, D.-Ivt., Ilmu Kesehatan, F., Muhammadiyah Sidoarjo, U., Rame Pilang No, J., Sidoarjo, W., & Timur, J. (2018). Original Research Articles Effectiveness of Red Betel Leaves Extract (Piper crocatum) Formulation with Fragrant Pandan Leaves Extract (Pandanus amaryllifolius Roxb.) Against Hyperglycemia and Pancreatic Histopathology of Mice. Journal of Medical Laboratory Science/ Technology).
15. Prayitno, S. A., Kusnadi, J. K., & Murtini, E. S. (2018). Karakteristik (Total Flavonoid, Total Fenol, Aktivitas Antioksidan) Ekstrak Serbuk Daun Sirih Merah (Piper crocatum Ruiz & Pav.). Foodscitech, 1(2), 26
16. Prayitno, S. A., Kusnadi, J. K., & Murtini, E. S. (2018). Karakteristik (Total Flavonoid, Total Fenol, Aktivitas Antioksidan) Ekstrak Serbuk Daun Sirih Merah (Piper crocatum Ruiz & Pav.). Foodscitech, 1(2).
17. Handajani, F. (2021). Revisi layout buku metode memilihan dan pembuatan hewan.
18. Che, H., Wang, Y., Li, H., Li, Y., Sahil, A., Lv, J., Liu, Y., Yang, Z., Dong, R., Xue, H., & Wang, L. (2020). Melatonin alleviates cardiac fibrosis via inhibiting lncRNA MALAT1/miR-141-mediated NLRP3 inflammasome and TGF-β1/Smads signaling in diabetic cardiomyopathy. FASEB Journal, 34(4)
19. Rini Hasanah Putri, C., Sunaryati, T.(2018). Pengaruh ekstrak tamarindus indica terhadap kenaikan berat badan pada tikus rattus novergicus yang diberi diet obesitas. Jurnal ilmiah Kedokteran Wijaya Kusuma (Vol. 6, Issue 1).
20. Noyes, A. M. (2014). Cardiac adipose tissue and its relationship to diabetes mellitus and cardiovascular disease. World Journal of Diabetes, 5(6), 868.
21. Harvey, P. A., & Leinwand, L. A. (2011). Cellular mechanisms of cardiomyopathy. In Journal of Cell Biology (Vol. 194, Issue 3, pp. 355–365).
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