Uji Efektivitas Ekstrak Lengkuas (Alpinia Galanga) Sebagai Antibakteri Escherichia Coli
Abstract
Food is a source of nutrition for humans and is also a source of food for microorganisms. The growth of microorganisms in foodstuffs can cause unwanted physical or chemical changes causing food to be bad for consumption. Therefore to maintain the quality, the food must be given of preservatives. However, people may misuse the preservatives leading to the low safety of food that threatens health. Natural preservatives of plants is more advisable to minimize the risk. The aim of this research was to see the effect of galangal extract (Alpinia galanga) using 60% ethyl acetate solvent in inhibiting the growth of Escherichia coli bacteria through diffusion disk method. The measured parameter was the inhibition zone formed around the disc paper with a 24-hour incubation period. This research was an experimental study by making galangal extract in certain concentrations. Based on the measurements, the average inhibitory zone diameter at three repetitions with 24 hours observation showed that the galangal extract in several concentrations effectively inhibited the growth of Escherichia coli bacteria. The result showed that the higher the concentration of the extract the larger the mean drag zone formed around the disk paper. The most effective concentration was 20 ml concentration with 25,6 mm inhibition (0.000 (P <0,05), F = 89,592).
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References
Irianto, K dan Waluyo, K., 2004. Gizi dan Pola Hidup Sehat. Yrama Widya: Bandung
Berg, J.M., Tymoczko, J.L., Stryer, L (2002). Molecular Cell Biology (5th ed). WH Freeman. ISBN 0-7167-4955-6
BPOM., 2011. Laporan Tahunan BPOM RI Tahun 2011.
BPOM., 2015. Laporan Tahunan BPOM RI Tahun 2015.
Wahyunigsih, Sri. "Penderita Diare di Kota Kupang Masih Tinggi”. http://m.harnas.co/2017/08/03/ penderitadiare-di-kota-kupang-masih-tinggi. diakses (20 Oktober 2017).
Jawetz, E, J. Melnick, et al., 2005, Mikrobiologi Kedokteran, Edisi 20, 238 –240, EGC, Jakarta.
Hapsari S., 2010. Pengaruh ekstrak jahe (Zingiber officianale) terhadap penghambat mikroba perusak pada ikan nila (Oreochromis niloticus), Surakarta.
Udjiana, S., 2008. Jurnal upaya pengawetan makanan menggunakan ekstrak lengkuas. teknologi separasi,1(2).
Parwata, M.O.A. dan Dewi, P.F.S., 2008. Isolasi dan Uji Aktivitas Antibakteri Minyak Atsiri dari Rimpang Lengkuas (Alpinia galanga L.). Jurnal kimia, 2(2), pp.100–104.
Nazir, M. (2003). Pola rancangan acak lengkap non faktorial. Pola rancangan acak lengkap non faktorial (Vol. 6).
Midun., 2012. Uji Efektivitas Ekstrak Lengkuas Merah (Alpinia purpurata K. Schum) dalam Menghambat Pertumbuhan Bakteri Staphylococcus aureus dan Bakteri Escherichia coli dengan Metode Disc Diffusion.
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