MOTILITAS DAN VIABILITAS SPERMATOZOA BABI DENGAN HOLDING TIME DAN LAMA THAWING BERBEDA (Sperm motility and viability of boars in different holding and thawing time)

  • Ni Luh Manik Sugiantini(1)
    Animal Husbandry of Udayana University
  • Ni Luh Gde Sumardani(2*)
    Universitas Udayana
  • I Wayan Suberata(3)
    Animal Husbandry of Udayana University
    • (*) Corresponding Author
DOI: https://doi.org/10.35508/nukleus.v10i2.11175

Abstract

The motility and viability of sperm are the main factors in determining semen quality. The treatment of semen before and after dilution is a determining factor whether or not the semen is suitable for use in artificial insemination. This study aims to determine the effect of holding time and thawing time on the motility and viability of boars sperm. This research was conducted at the Livestock Reproduction Laboratory, Bukit Jimbaran for 1 month, using a factorial randomized block design The first factor is the holding time which consists of 3 levels: 0 hours (HT0), 2 hours (HT2), and 3 hours (HT3). The second factor is the thawing time which consists of 3 levels: for 2 minutes (T2), 3 minutes (T3), and 5 minutes (T5). There are 9 combinations and 5 groups based on sperm storage time. The observed variables included the motility and viability of boars sperm. The results showed that the HT3 treatment was significantly different (P<0.05) with 22% sperm motility and 41.55% sperm viability. Thawing for 5 minutes (T5) gave the best results with sperm motility 18.50% and sperm viability 38.45%. There was no interaction between holding time and thawing time. The conclusion of this study is that boars sperm shows the best results at holding time of 3 hours with a thawing time of 5 minutes.

Downloads

Download data is not yet available.

References

Anwar P , Jiyanto J. 2019. Efektivitas sukrosa sebagai proteksi aktif membran ekstraseluler spermatozoa Sapi Bali pada zona pre-freezing. Jurnal Agripet. 19(1): 77-84. https://doi.org/10.17969/agripet.v19i1.14468
Arifiantini RI. 2012. Teknik Koleksi dan Evaluasi Semen Pada Hewan. PT Penerbit IPB Press. Bogor.
Bearden HJ, Fuquay F, Willard ST. 2004. Applied Animal Reproduction. 6th Ed. Pearson Prentaice Hallm. New Jersey. USA.
Bebas W, Buyona GL, Budiasa MK. 2016. Penambahan vitamin E pada pengencer BTS® terhadap daya hidup dan motilitas spermatozoa babi landrace pada penyimpanan 15°C. Buletin Veteriner Udayana. 8(1): 1-7. https://ojs.unud.ac.id/index.php/buletinvet/article/download/19665/13056
Fadilah ZN, Isnaini N, Ihsan MN. 2016. Kualitas semen cair Sapi Bali selama penyimpanan suhu ruang menggunakan pengencer skim milk dengan penambahan filtrat kecambah kacang hijau. Ternak Tropika Journal of Tropical Animal Production. 17(1): 22-30. https://doi.org/10.21776/ub.jtapro.2016.017.01.3
Fiqri MM. 2014. Motilitas Spermatozoa Sapi Brahman dengan berbagai konsentrasi dalam engencer CEP-D yang disimpan dalam refrigerator. Lentera Bio. 3(3): 181-185. https://ejournal.unesa.ac.id/index.php/lenterabio/article/view/9611
Garner DL, Hafez ESE. 2000. Spermatozoa and Seminal Plasma. In: Hafez B. Hafez ESE, editor. Reproduction in Farm Animals. 7th Ed. Philadelphia (US): Lippincott Williams and Wilkins.
Indriani TS, Wahyuningsih S. 2013. Daya hidup spermatozoa Sapi Limousin yang dipreservasi dengan metode water jacket dan free water jacket. Jurnal Veteriner September. 14(3): 379-386. https://ojs.unud.ac.id/index.php/jvet/article/view/7276
Munazaroh A M, Wahyuningsih S, Ciptadi G. 2013. The quality of boer goat freezing sperms using mr. frosty equipments with different Andromed equilibration. Jurnal Ternak Tropika. 14(2): 63-71.
Pratiwi RI, Suharyati S, Hartono M. 2014. Analisis kualitas semen beku Sapi Simmental menggunakan pengencer Andromed® dengan variasi waktu pre freezing. Jurnal Ilmiah Peternakan Terpadu. 2(3): 8-15. https://jurnal.fp.unila.ac.id/index.php/JIPT/article/view/496
Rienprayoon CC, Klangnak S, Onton C, Tretipskul, Tummaruk P. 2012. A comparative study on the efficacy of four semen extenders and thawing by seminal plasma on the quality of frozen thawed boar semen. The Thai Journal of Veterinary Medicine. 42(2): 195-200. https://digital.car.chula.ac.th/tjvm/vol42/iss2/9
Sades AM, Isnaini N, Wahjuningsih S. 2016. Pengaruh suplementasi filtrat kecambah kacang hijau (Phaseolus radiatus L.) terhadap kualitas semen Sapi Simmental dalam pengencer skim milk pada suhu dingin. Ternak Tropika Journal of Tropical Animal Production. 17(1): 1-10. https://doi.org/10.21776/ub.jtapro.2016.017.01.1
Savitri FK, Suharyati S. 2014. Kualitas semen beku Sapi Bali dengan penambahan berbagai dosis vitamin C pada bahan pengencer skim kuning telur. Jurnal Ilmiah Peternakan Terpadu. 2(3): 30-36. https://jurnal.fp.unila.ac.id/index.php/JIPT/article/view/499
Simarmata YNS, Sumardani NLG, Artiningsih RN. 2018. Effect of thawing length on quality of boar semen attechnical service unit of artificial insemination centre Bali Province. Jurnal Peternakan Tropik. 6(2): 501-508. https://ojs.unud.ac.id/index.php/tropika/article/view/41841
Solihati N, Rasad SD, Setiawan R, Nurjanah S. 2018. Pengaruh kadar gliserol terhadap kualitas semen domba lokal. Jurnal Biodjati. 3(1): 63-71. https://doi.org/10.15575/biodjati.v3i1.2357
Steel RGD, Torrie JH. 1993. Prinsip dan Prosedur Statistika (Pendekatan Biometrik). Penerjemah B. Sumantri. Gramedia Pustaka Utama, Jakarta.
Stradivari MPF, Sumardani NLG, Mariani NP. 2019. Prosesing semen beku di Unit Pelaksana Teknis (UPT) Balai Inseminasi Buatan Daerah Provinsi Bali. Jurnal Peternakan Tropika. 7(1): 163-168. https://ojs.unud.ac.id/index.php/tropika/article/view/47298
Susilowati S, Triana IN. 2016. Protein kasar plasma seminalis sapi menurunkan kejadian nekrosis spermatozoa kambing yang disimpan pada suhu dingin. Jurnal Veterine., 17(1): 57-63. http://dx.doi.org/10.19087/jveteriner.2016.17.1.57
Tuty LY, Arifiantini RI, Dapawole RR, Nalley WMM. 2017. Kualitas semen beku babi dalam pengencer komersial yang disuplementasi dengan Trehalosa. Jurnal Veteriner. 18(1): 69-75. https://ojs.unud.ac.id/index.php/jvet/article/view/29655
Wasilewska K, Fraser L. 2017. Boar variability in sperm cryo-tolerance after cooling of semen in different long-term extenders at various temperatures. Ani Reprod Sci, 185(-): 161-173. https://doi.org/10.1016/j.anireprosci.2017.08.016
Zhang M, Oldenhof H, Sieme H, Wolkers WF. 2016. Freezing-induced uptake of trehalose into mammalian cells facilitates cryopreservation. Biochimica et Biophysica Acta (BBA)-Biomembranes. 1858(6): 1400-1409. https://doi.org/10.1016/j.bbamem.2016.03.020

PlumX Metrics

Published
2023-12-26
How to Cite
Sugiantini, N. L., Sumardani, N. L., & Suberata, I. W. (2023). MOTILITAS DAN VIABILITAS SPERMATOZOA BABI DENGAN HOLDING TIME DAN LAMA THAWING BERBEDA (Sperm motility and viability of boars in different holding and thawing time). JURNAL NUKLEUS PETERNAKAN, 10(2), 9-16. https://doi.org/10.35508/nukleus.v10i2.11175
Issue
Vol 10 No 2 (2023): Desember
Section
Articles

Copyright (c) 2023 JURNAL NUKLEUS PETERNAKAN

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.