KUALITAS SEMEN BEKU SAPI BALI PASCA THAWING DALAM WAKTU BERBEDA (Quality of Bali Bull Frozen Semen Post Thawing in Differents Times)
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Fafandri .(1)
Universitas Udayana
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Ni Luh Gde Sumardani(2*)
Universitas Udayana
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I Wayan Suberata(3)
Universitas Udayana
(*) Corresponding Author
Abstract
The aim of this research was to know quality of bali bull frozen semen post thawing in defferent times. The research was conducted in Livestock Reproduction Laboratory of the Faculty of Animal Husbandry, Udayana University, Bukit Jimbaran, bali Province in one months. The treatment of this research are T 1 (thawing in 15 seconds), T 2 (thawing in 30 seconds), and T3 (thawing in 60 seconds) on temperatur 37oC. The data of research was sperm motility, sperm viability, and sperm abnormality. Result of this research showed that the quality of bali bull frozen semen post thawing in defferent times that 15 second (T 1), 30 second (T 2), and 60 second (T 3) did not significantly (P> 0.05). The best thawing is at T3 treatment with sperm motility 71.00±2.00%, sperm viability 56.07±1.69%, and sperm abnormality 1.97±0.47%. The conclusion of this study is the bali bull frozen semen can be thawed at temperature 37oC in 60 seconds.
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References
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https://doi.org/10.19087/jveteriner.2019.20.3.324
Sundari T W, Tagama TR, Maidaswar. 2013. Korelasi kadar pH semen segar dengan kualitas semen sapi limousin di Balai Inseminasi Buatan. Jurnal Ilmu Peternakan 1(3): 1043-1049. http://jos.unsoed.ac.id/index.php/jip/article/view/691
Susilawati T. 2013. Pedoman Inseminasi Buatan Pada Ternak. Universitas Brawijaya (UB) Press, Malang.
Suwiti N K, Besung INK, Mahardika GN. 2017. Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia. Veterinary World 10(10): 1250-1254. http://www.veterinaryworld.org/Vol.10/October-2017/14.html
Varasofiari L N, Setiatin ET, Sutopo. 2013. Evaluasi kualitas semen segar sapi jawa brebes berdasarkan lama waktu penyipanan. Animal Agriculture 2(1):201-208. https://ejournal3.undip.ac.id/index.php/aaj/article/view/2163
Udin. 2012. Teknologi Inseminasi Buatan dan Transfer Embrio pada Sapi. Penerbit Sukabina Press, Padang.
Zulyazaini, Dasrul, Wahyuni S, Akmal M, Abdullah MAN. 2016. Karakteristik semen dan komposisi kimia plasma seminalis sapi aceh yang diperihara di BIBD Saree Aceh Besar. Agripet, 16(2):121-130. https://doi.org/10.17969/agripet.v16i2.5803
Zulkharnaim, Jakaria, Noor RR. 2010. Identifikasi keragaman genetik gen reseptor hormon pertumbuhan (GHR Alu I) pada sapi bali. Media Peternakan. 33(2): 81-87. https://doi.org/10.5398/medpet.2010.33.2.81
Ansary M S, Bushra A, Rakha, Akher S. 2010. Effect of straw size and thawing time on quality of cryopreserved buffalo (Bbubalus bubalis) semen. J Reproductive Biology. 11(1): 49-54. https://doi.org/10.1016/S1642-431X(12)60063-1
Arifiantini R L. 2012. Teknik Koleksi dan Evaluasi Semen Pada Hewan. IPB Press. Bogor.
Azzahra F Y, Setiatin ET , Samsudewa D. 2016. Evaluasi motilitas dan pesentase hidup semen segar sapi PO Kebumen pejantan muda. Jurnal Sains Peternakan Indonesia, 11(2):99-107. https://doi.org/10.31186/jspi.id.11.2.99-107
Ax R L, Dally MR, Didion BA, Lenz RW, Love CC, Varner DD, Hafez B , Bellin ME. 2000. Semen Evaluation. In ESE Hafez (ed). Reproduction in Farm Animal, 7th Edition. USA: Lippincott Williams and Wilkins. P 365-375.
Bardan, Feradis , Adelina T. 2009. Penggunaan air tebu yang dikombinasidengan kuning telur sebagai pengencer semen sapi bali. Jurnal Peternakan. 6(2): 36-43. https://ejournal.uin-suska.ac.id/index.php/peternakan/article/viewFile/371/354
Campbell J R, Campbell KL, Kenealy MD. 2003. Artificial Insemination: in Animal Sciences Ed 4th. Mc Graw-Hill, New York.
Chenoweth P J. 2005. Genetic sperm defect. Theriogenology. 64(3): 457-468. https://doi.org/10.1016/j.theriogenology.2005.05.005
Contri A, Gloria A, Robbe D, C Valorz C, Wegher L, Carluccio A. 2013. Kinematic study on the effect of pH on bull sperm function. Animal Reproduction Science 136(4): 252-259. https://doi.org/10.1016/j.anireprosci.2012.11.008
Feradis. 2010. Bioteknologi Reproduksi pada Ternak. Alfabeta. Bandung.
Garner, D L, Hafez E S E. 2000. Spermatozoa and Seminal Plasma. In E S E Hafez (ed). Reproduction in Farm Animal, 7th Edition. USA: Lippincott Williams and Wilkins.
Graham J K. 2000. Assessment of Sperm Quality. Proceedings of the Annual Convention of the AAEP AAEP. 47: 302-305. https://doi.org/10.1016/S0378-4320(01)00160-9
Hafez B, Bellin M E, Varner D D, Love C C, Lenz R W, Didion B A, Dally M, Ax R L. 2000a Semen Evaluation. In Reproduction in Farm Animals. 7th Ed. Philadelphia, USA: Lea dan Febiger.
Hafez E S E , Garner D L. 2000b. Spermatozoa and Seminal Plasma. In Reproduction in Farm Animals. 7th Ed. Philadelphia, USA: Lea dan Febiger.
Hartanti D, Setiatin ET, Sutopo. 2012. Perbandingan penggunaan pengencer semen sitrat kuning telur dan tris kuning telur terhadap persentase daya hidup spermatozoa sapi jawa brebes. Animal Agri. Jour.1 (1): 33-42. https://ejournal3.undip.ac.id/index.php/aaj/article/view/89
Ismaya. 2014. Bioteknoloi Inseminasi Buatan pada Sapi dan Kerbau. Gajah Mada University Press,Yogyakarta.
Kusumawati E D, Krisnaningsih ATN, Romadlon R R. 2016. Kualitas spermatozoa semen beku sapi simental dengan suhu dan lama thawing yang berbeda. Jurnal Ilmu-Ilmu Peternakan, 26(3): 38-41. https://doi.org/10.21776/ub.jiip.2016.026.03.06
Lestari S, Saleh,D M, Maidaswar. 2013. Profil kualitas semen segar sapi pejantan limousin dengan umur yang berbeda di Balai Inseminasi Buatan Lembang Jawa Barat. Jurnal Ilmu Peternakan. 1(3): 1165- 1172. http://dx.doi.org/10.35792/zot.40.2.2020.28438
Nirwana , Suparman. 2017. The effect of males age on the quality of bali cattle fresh semen. Journal of Animal Husbandry 2(2): 13-18. https://doi.org/10.31327/chalaza.v2i2.296
Parkinson T J. 2004. Review: Evaluation of fertility and infertility in natural service bulls. Vet. J. 168: 215-229. https://doi.org/10.1016/j.tvjl.2003.10.017
Pesch S, Hoffman B. 2007. Cryopreservation of spermatozoa in veterinary medicine. J. Reproduktionsmed. Endokrinol. 4(2): 101—105. https://www.kup.at/kup/pdf/6429.pdf
Salim M A, Susilawati T, Wahjuningsih S. 2012. Pengaruh metode thawing terhadap kualitas semen beku sapi bali, sapi madura dan sapi PO. Jurnal Agripet. 12(2): 14-19. https://doi.org/10.17969/agripet.v12i2.197
Standar Nasional Indonesia. 2017. Semen beku sapi (4869.1).
https://www.bsn.go.id/main/bsn/isi_bsn/20004/sni. Diakses 9 Desember 2022.
Sunami, Isnaini SN , Wahjuningsih S. 2017. Kualitas semen segar dan recovery rate (RR) sapi limousin pada musim yang berbeda. J. Ternak Tropika. 18(1): 36-50. https://doi.org/10.21776/ub.jtapro.2017.018.01.6
Sumardani N L G, Tuty L Y, Siagian P H. 2008. The boar sperm viability in modified BTS (Beltsville Thawing Solution) in different storage. Media Peternakan. 31(2): 81-86.
https://jurnal.ipb.ac.id/index.php/mediapeternakan/article/view/1085
Sumardani N L G, Budaarsa K, Putri TI, Puger AW. 2019. Umur memengaruhi volume semen dan motilitas spermatozoa babi landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali. Jurnal Veteriner 20(3): 324-329.
https://doi.org/10.19087/jveteriner.2019.20.3.324
Sundari T W, Tagama TR, Maidaswar. 2013. Korelasi kadar pH semen segar dengan kualitas semen sapi limousin di Balai Inseminasi Buatan. Jurnal Ilmu Peternakan 1(3): 1043-1049. http://jos.unsoed.ac.id/index.php/jip/article/view/691
Susilawati T. 2013. Pedoman Inseminasi Buatan Pada Ternak. Universitas Brawijaya (UB) Press, Malang.
Suwiti N K, Besung INK, Mahardika GN. 2017. Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia. Veterinary World 10(10): 1250-1254. http://www.veterinaryworld.org/Vol.10/October-2017/14.html
Varasofiari L N, Setiatin ET, Sutopo. 2013. Evaluasi kualitas semen segar sapi jawa brebes berdasarkan lama waktu penyipanan. Animal Agriculture 2(1):201-208. https://ejournal3.undip.ac.id/index.php/aaj/article/view/2163
Udin. 2012. Teknologi Inseminasi Buatan dan Transfer Embrio pada Sapi. Penerbit Sukabina Press, Padang.
Zulyazaini, Dasrul, Wahyuni S, Akmal M, Abdullah MAN. 2016. Karakteristik semen dan komposisi kimia plasma seminalis sapi aceh yang diperihara di BIBD Saree Aceh Besar. Agripet, 16(2):121-130. https://doi.org/10.17969/agripet.v16i2.5803
Zulkharnaim, Jakaria, Noor RR. 2010. Identifikasi keragaman genetik gen reseptor hormon pertumbuhan (GHR Alu I) pada sapi bali. Media Peternakan. 33(2): 81-87. https://doi.org/10.5398/medpet.2010.33.2.81
Published
2023-12-30
How to Cite
., F., Sumardani, N. L., & Suberata, I. W. (2023). KUALITAS SEMEN BEKU SAPI BALI PASCA THAWING DALAM WAKTU BERBEDA (Quality of Bali Bull Frozen Semen Post Thawing in Differents Times). JURNAL NUKLEUS PETERNAKAN, 10(2), 52-59. https://doi.org/10.35508/nukleus.v10i2.11176
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