KARAKTERISTIK SEMEN CAIR KALKUN DALAM MEDIA PENGENCER RINGER LAKTAT KUNING TELUR (Characteristics of Turkey’s Liquid Semen in Ringer Lactate Egg Yolk Diluent)
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Ichsan Pamungkas(1)
Universitas Djuanda
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Annisa Rahmi(2*)
Universitas Djuanda
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Ristika Handarini(3)
Universitas Djuanda
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Abdullah Baharun(4)
Universitas Djuanda
(*) Corresponding Author
Abstract
Turkey is a poultry with high economic value, an animal protein resource with high nutrition. Limitations in natural mating cause the small population of turkeys in Indonesia. Therefore, artificial insemination will be a solution to this problem. This research aims to know the diluent effectivity of ringer lactate and egg yolk to preserve turkey’s liquid semen stored at a temperature of 4 °C. The fresh semen from 2 bronze breed turkeys, ages 8 and 12 months, was collected with massage method. This research uses a complete randomized design with four treatment groups for each storage period: T0= storage for 0 hours, T1 = storage for 24 hours, T2 = storage for 48 hours, and T4 = storage for 72 hours with six repetitions for each group. The parameters observed from fresh semen are volume, color, pH, consistency, mass movement, motility, viability, and abnormality. The fresh semen was processed into liquid semen using ringer lactate egg yolk diluent with a ratio of 4:1 and then stored at a temperature of 4 °C. The parameters observed from liquid semen are motility, viability, abnormality, and recovery rate. The data obtained were analyzed using analysis of variance, and in case there were significant differences between treatments, the test was continued with the Duncan test. The results showed that ringer lactate egg yolk diluent can preserve turkey’s liquid semen quality until storage for 48 hours. We can conclude the turkey’s liquid semen in ringer lactate egg yolk diluent can be used for artificial insemination maximum of 48 hours of storage.
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References
Aji RN. 2015. Pengaruh umur terhadap kualitas sperma kalkun (Meleagris gallopavo). Skripsi .Yogyakarta: Fakultas Ilmu dan Industri Peternakan, Universitas Gadjah Mada.
Azizah N, Komarudin, Pratiwi N, Kostaman T, Sartika T. 2023. Analisis kualitas semen ayam lokal indonesia berdasarkan galur dan umur dewasa kelamin yang berbeda. Jurnal Agripet 1 (23): 40-45.
Baharun A, Said S, Arifiantini I . 2021. Correlation between age, testosterone and adiponectin concentration and sperma abnormalities in simental bulls. Veterinary World 14 (8): 2124-2130.
Chenoweth PJ. 2005. Genetic sperm defect. Theriogenology. 64(3): 457-468. https://doi.org/10.1016/j.theriogenology.2 005.05.005
Danang D, Isnaini, Trisunuwati. 2012. Pengaruh lama simpan semen terhadap kualitas spermatozoa ayam kampung dalam pengencer ringer’s pada suhu 4°C. Ternak Tropika Journal of Tropical Animal Production: 13(1), 47–57.
Ervandi M, Ardiansya W, Prahara S. 2020. Kualitas dan fertilitas spermatozoa sebagai akibat pejantan berbeda. Jambura journal of animal science. 2 (2): 29-37.
Ferianto FE, Setiawan I, Tanwiriah W. 2015. Identifikasi sifat-Sifat kuantitatif kalkun (Meleagris gallopavo) jantan dan betina dewasa. Students e-Journal 4 (3): 1-8.
Ghirardosi MS, Fischman ML, Jorge AE, Chan D, Cisale A. 2018. Relationship between morphological abnormalities in commercial bull frozen semen doses and conception rate. Andrologia 50(3): e12884
Harferri KT, Nurmeiliasari, Putranto HD. 2020. Studi kualitas semen ayam burgo. Buletin Ternak Tropis 1 (1): 10- 15.
Hidayat C, Sopiyana S, Rahman R. 2020. Review: Pengaruh pakan terhadap kualitas semen ayam. Jurnal Ilmu dan Teknologi Peternakan Tropis 7(3):218-232.
Hoesni F. 2013. Pengaruh penggunaan metode thawing yang berbeda terhadap kualitas spermatozoa semen sapi perah berpengencer tris sitrat kuning telur. Jurnal Ilmiah Universitas Batanghari Jambi 3(4):118-126.
Kucera AC, Heidinger BJ. 2018. Avian semen collection by cloacal massage and isolation DNA from sperm. Journal of Visualized Experiments 132 (e55324):1-5.
Lukman HY, Burhan, Nikmaturrayan, Karni I, Khoirani K. 2022. Inseminasi buatan menggunakan sperma beku pada ternak sapi bali untuk meningkatkan mutu genetik ternak di kecamatan woha kabupaten bima. Indonesia Journal of Education and Community Services 2 (1): 132-138.
Modupe O, Livinus AC, Ifeanyi NB. 2013. Semen quality characteristics and effect of mating ratio on reproductive performance of Hubbard broiler breeders. Journal of Agricultural Science 5 (1): 154-159.
Mphaphathi ML, Seshoka MM, Luseba D, Sutherland B, Nedambale TL. 2016. The characterization and cryopreservation of Venda chicken semen. Asian Pacific Journal of Reproduction 5(2):132–139.
Pambudi JR, Budiasa MK, Bebas W. 2015. Dosisi glukosa ideal pada pengencer kuning telur fosfat dalam mempertahankan kualitas semen kalkun pada suhu 5°C. Indonesia Medicus Veterinus 4 (2): 104-110.
Pandia YM, Bebas W, Pemayun TGO. 2021. Motilitas dan daya hidup spermatozoa ayam cemani dalam pengencer ringer laktat kuning telur pada penyimpanan suhu 4°C. Indonesia Medicus Veterinus 10 (1): 105-115.
Parkinson TJ. 2004. Review: Evaluation of fertility and infertility in natural service bulls. Vet. J. 168: 215-229. https://doi.org/10.1016/j.tvjl.2003.10.017
Putranto H, Nurmeiliasari, Harferry K. 2020. Studi kualitas semen ayam Burgo. Bull Trop Anim Sci 1(1):10–15.
Raheja N, Choudhary S, Grewal S, Sharman N, Kumar N. 2018. Review on semen extenders and additives used in cattle and buffalo bull semen preservation. Journal of Entomology and Zoology Studies 6 (3): 239-245.
Rohlyharni E, Atabany A, Purwanto BC. 2023. Produksi dan kualitas semen calon pejantan unggul sapi perah uji zuriat di BIB Lembang. Jurnal Sains dan Teknologi Peternakan 4(2): 63–71.
Saleh DM, Sumaryadi MY, Nugroho AP, Hidayah CN. 2020. Penggunaan pengencer standar pada semen ayam kampung. Prosiding Seminar Nasional Teknologi Agribisnis Peternakan (STAP) 7:539-544.
Sari NMDP, Bebas W, Trilaksana IGNB. 2015. Madu meningkatkan kualitas semen kalkun selama penyimpanan. Buletin Veteriiner Udayana 7 (2): 164-171.
Setyawan E, Yaman MA, Abdullah MAN. 2022. Efek substitusi kombinasi tepung maggot (Hermetia illucens) dan sprouted fodder for chicken (SF2C) dalam pakan fermentasi terhadap kualitas makroskopis dan mikroskopis sperma ayam jantan Fi hasil persilangan ayam lokal dan ayam brahma. Jurnal ilmiah Mahasiswa Pertanian. 7(2): 279-292.
Sofa RN, Rasad SD, Setiawan I. 2022. Pengaruh level kuning telur dalam pengencer tris terhadap viabilitas dan motilitas sperma semen entog (Cairina moschata). Jurnal Produksi Ternak Terapan. 3 (2): 64-72.
Susilawati, T. 2013. Pedoman Inseminasi Buatan pada Ternak. Universitas Brawijaya (UB) Press: Malang. ISBN 978-602-203-458-2.
Tethool AN, Ciptadi G, Wahjuningsih S, Susilawati T. 2022. Karakteristik dan jenis pengencer semen sapi bali: suatu review. Jurnal Ilmu Peternakan dan Veteriner Tropis 12 (1): 45-57.
Toelihere MR. 1993. Inseminasi Buatan pada Ternak. PT. Angkasa: Bandung.
Yuniar TU, Saleh DM, Mugiyono S. 2021. Pengaruh penambahan kuning telur pada pengencer susu skim dan lama penyimpanan pada suhu 5oC terhadap kualitas spermatozoa ayam pelung. Journal of Animal Science and Technology. 3 (1): 29-46.
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