PENGENCER SEMEN YANG DIPERKAYA MINYAK IKAN MENJAGA KUALITAS SPERMATOZOA BABI (Sus scrofa domesticus) SELAMA PENYIMPANAN JANGKA PENDEK Fish Oil Enriched Semen Extender Protect Boar (Sus scrofa domesticus) Spermatozoa Quality During Short Term Storage
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Parsaoran Silalahi(1*)
Universitas HKBP Nommensen Medan
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Pohan Panjaitan(2)
Universitas HKBP Nommensen
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Imelda Tafonao(3)
Universitas HKBP Nommensen
(*) Corresponding Author
Abstract
This study aimed to evaluate the effect of fish oil addition to semen extender on the quality of boar spermatozoa during 24-hour storage at 15°C. Fresh semen was collected from a healthy adult boar with initial motility ≥70% and diluted using a glukosa–sitrat–EDTA extender supplemented with 0 g (P0), 0.15 g (P1), 0.30 g (P2), and 0.45 g (P3) of fish oil per 1.000 mL. The experiment was arranged in a completely randomized design (CRD) with four treatments and four replications. Parameters observed included motility, viability, abnormality, and semen pH after 24-hour storage. Analysis of variance showed that fish oil addition had a highly significant effect (P<0.01) on sperm viability, but no significant effects (P>0.05) on motility, abnormality, or pH. The highest viability was found in P1 (93.25%) and the lowest in P3 (82.00%). Motility and abnormality improved at low doses but declined at higher concentrations. The pH values remained stable (6.7–7.2) across all treatments. In conclusion, adding a low dose of fish oil (0.15 g/1.000 mL) effectively maintained spermatozoa quality without altering extender chemistry, suggesting its potential as a natural additive for semen extender formulations in tropical artificial insemination programs.
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References
Aitken, R.J., & Curry, B.J. (2011). Redox regulation of human sperm function: From the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line. Antioxidants dan Redox Signaling, 14(3), 367–381. https://doi.org/10.1089/ars.2010.3186
Alvarez, J.G. and Aitken, R.J., (2012). Lipid peroxidation in human spermatozoa. In Studies on Men's Health and Fertility (pp. 119-130). Totowa, NJ: Humana Press.
Arsadana, I.K.A., Sumardani, N.L.G., Trilaksana, I.G.N.B., & Bebas, W. (2025). Motilitas, viabilitas dan abnormalitas spermatozoa Babi Landrace yang diencerkan menggunakan pengencer berbasis air buah lontar dengan tambahan kuning telur dan ekstrak daun kelor. Buletin Veteriner Udayana, 17(2), 463–474. https://doi.org/10.24843/bulvet.2025.v17.i02.p24
Badan Pusat Statistik Republik Indonesia. (2024). Produksi Daging Babi Menurut Provinsi Tahun 2024. Jakarta: BPS RI.
Blagojević, J., Petrović, A., & Stanković, B. (2024). Impact of supplemented nutrition on semen quality in boars: Antioxidant-based improvements in sperm kinetics and morphology. Animals (MDPI), 14(1), 115–128. https://doi.org/10.3390/ani14010115
CNBC Indonesia. (2024). Populasi Babi di Indonesia Turun Signifikan Akibat African Swine Fever.
Collodel, G., Castellini, C., Lee, J.C., & Signorini, C. (2020). Relevance of Fatty Acids to Sperm Maturation and Quality. Oxid Med Cell Longev., 5:2020:7038124. https://doi.org/10.3390/ijms21124359
FAO. (2021). Guidelines for Animal Welfare in Research and Education. Food and Agriculture Organization of the United Nations, Rome.
Galić, I., Dragin, S., Stančić, I., Maletić, M., Apić, J., Kladar, N., Spasojević, J., Grba, J., & Kovačević, Z., (2022). Effect of an antioxidant supplement combination on boar sperm. Animals, 12(10), p.1301. https://doi.org/10.3390/ani12101301
Hsu, W., Lin, T., Chang, S., Lin, M., Huang, C., Shen, P., Chou, C., & Peng, S., (2025). Optimization of Black Boar Sperm Cryopreservation Efficiency with Antioxidant-Rich Plant Extracts from Djulis (Chenopodium formosanum). Animals, 15(12), p.1798. https://doi.org/10.3390/ani15121798
Jensen, T.K., Priskorn, L., Holmboe, S.A., Nassan, F.L., Andersson, A.M., Dalgård, C., Petersen, J.H., Chavarro, J.E., & Jørgensen, N. (2020). Associations of fish oil supplement use with testicular function in young men. JAMA Network Open, 3(1), pp.e1919462-e1919462. https://doi.org/10.1001/jamanetworkopen.2019.19462
Khophloiklang, V., Chanapiwat, P., & Kaeoket, K. (2024). Crude Garden Cress Seed Oil (Lepidium sativum Linn.) Enhances Post-Thawed Boar Sperm Quality. Animals, 14(22), 3178. https://doi.org/10.3390/ani14223178
Liu, Q., Duan, R.J., Zhou, Y.F., Wei, H.K., Peng, J., & Li, J.L., (2017). Supplementing oregano essential oil to boar diet with strengthened fish oil: Effects on semen antioxidant status and semen quality parameters. Andrologia, 49(10), p.e12764. https://doi.org/10.1111/and.12764
Naibina, M.P., Bebas, W., Trilaksana, I.G.N.B., Pemayun, T.G.O., & Laksmi, D. N. (2025). Quality of boar semen extended in palm water–egg yolk diluent supplemented with A. dorsata and Trigona sp. honey. Buletin Veteriner Udayana, 17(3), 811–823. https://doi.org/10.24843/bulvet.2025.v17.i03.p27
Sugiantini, N.L.M., Sumardani, N.L.G., & Suberata, I.W. (2023). Motilitas dan viabilitas spermatozoa babi dengan holding time dan lama thawing berbeda. Jurnal Nukleus Peternakan, 10(2), 9–16. https://doi.org/10.35508/nukleus.v10i2.11175
Sumardani, N.L.G., Bebas, W., Trilaksana, I.G.N.B., & Pemayun, T.G.O. (2019). Umur memengaruhi volume semen dan motilitas spermatozoa Babi Landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali. Jurnal Veteriner, 20(3), 324–329. https://doi.org/10.19087/jveteriner.2019.20.3.324
Sutriana, A., Hasibuan, M.A.L., Armansyah, T., Siregar, T.N., Panjaitan, B., Sayuti, A., & Aliza, D. (2021). Pemberian Ekstrak Akar Pasak Bumi Meningkatkan Kualitas Spermatozoa Domba Waringin. Jurnal Veteriner, 22(3), 317-323. https://doi.org/10.19087/jveteriner.2021.22.3.317
Tako, A.I., Nalley, W.M., Setyani, N.M.P., & Hine, T.M. (2025). Efek Penambahan Kuning Telur Pada Level yang Berbeda Dalam Pengencer Spermax Terhadap Kualitas Semen Beku Babi Landrace. Jurnal Ilmiah Ilmu-Ilmu Peternakan, 28(2), 199-210. https://doi.org/10.22437/jiiip.v28i2.44806
Waberski, D., Riesenbeck, A., Schulze, M., Weitze, K.F., & Johnson, L., (2019). Application of preserved boar semen for artificial insemination: Past, present and future challenges. Theriogenology, 137, 2-7. https://doi.org/10.1016/j.theriogenology.2019.05.030
Yuan, C., Wang, J., & Lu, W., (2023). Regulation of semen quality by fatty acids in diets, extender, and semen. Frontiers in Veterinary Science, 10, 1119153. https://doi.org/10.3389/fvets.2023.1119153
Alvarez, J.G. and Aitken, R.J., (2012). Lipid peroxidation in human spermatozoa. In Studies on Men's Health and Fertility (pp. 119-130). Totowa, NJ: Humana Press.
Arsadana, I.K.A., Sumardani, N.L.G., Trilaksana, I.G.N.B., & Bebas, W. (2025). Motilitas, viabilitas dan abnormalitas spermatozoa Babi Landrace yang diencerkan menggunakan pengencer berbasis air buah lontar dengan tambahan kuning telur dan ekstrak daun kelor. Buletin Veteriner Udayana, 17(2), 463–474. https://doi.org/10.24843/bulvet.2025.v17.i02.p24
Badan Pusat Statistik Republik Indonesia. (2024). Produksi Daging Babi Menurut Provinsi Tahun 2024. Jakarta: BPS RI.
Blagojević, J., Petrović, A., & Stanković, B. (2024). Impact of supplemented nutrition on semen quality in boars: Antioxidant-based improvements in sperm kinetics and morphology. Animals (MDPI), 14(1), 115–128. https://doi.org/10.3390/ani14010115
CNBC Indonesia. (2024). Populasi Babi di Indonesia Turun Signifikan Akibat African Swine Fever.
Collodel, G., Castellini, C., Lee, J.C., & Signorini, C. (2020). Relevance of Fatty Acids to Sperm Maturation and Quality. Oxid Med Cell Longev., 5:2020:7038124. https://doi.org/10.3390/ijms21124359
FAO. (2021). Guidelines for Animal Welfare in Research and Education. Food and Agriculture Organization of the United Nations, Rome.
Galić, I., Dragin, S., Stančić, I., Maletić, M., Apić, J., Kladar, N., Spasojević, J., Grba, J., & Kovačević, Z., (2022). Effect of an antioxidant supplement combination on boar sperm. Animals, 12(10), p.1301. https://doi.org/10.3390/ani12101301
Hsu, W., Lin, T., Chang, S., Lin, M., Huang, C., Shen, P., Chou, C., & Peng, S., (2025). Optimization of Black Boar Sperm Cryopreservation Efficiency with Antioxidant-Rich Plant Extracts from Djulis (Chenopodium formosanum). Animals, 15(12), p.1798. https://doi.org/10.3390/ani15121798
Jensen, T.K., Priskorn, L., Holmboe, S.A., Nassan, F.L., Andersson, A.M., Dalgård, C., Petersen, J.H., Chavarro, J.E., & Jørgensen, N. (2020). Associations of fish oil supplement use with testicular function in young men. JAMA Network Open, 3(1), pp.e1919462-e1919462. https://doi.org/10.1001/jamanetworkopen.2019.19462
Khophloiklang, V., Chanapiwat, P., & Kaeoket, K. (2024). Crude Garden Cress Seed Oil (Lepidium sativum Linn.) Enhances Post-Thawed Boar Sperm Quality. Animals, 14(22), 3178. https://doi.org/10.3390/ani14223178
Liu, Q., Duan, R.J., Zhou, Y.F., Wei, H.K., Peng, J., & Li, J.L., (2017). Supplementing oregano essential oil to boar diet with strengthened fish oil: Effects on semen antioxidant status and semen quality parameters. Andrologia, 49(10), p.e12764. https://doi.org/10.1111/and.12764
Naibina, M.P., Bebas, W., Trilaksana, I.G.N.B., Pemayun, T.G.O., & Laksmi, D. N. (2025). Quality of boar semen extended in palm water–egg yolk diluent supplemented with A. dorsata and Trigona sp. honey. Buletin Veteriner Udayana, 17(3), 811–823. https://doi.org/10.24843/bulvet.2025.v17.i03.p27
Sugiantini, N.L.M., Sumardani, N.L.G., & Suberata, I.W. (2023). Motilitas dan viabilitas spermatozoa babi dengan holding time dan lama thawing berbeda. Jurnal Nukleus Peternakan, 10(2), 9–16. https://doi.org/10.35508/nukleus.v10i2.11175
Sumardani, N.L.G., Bebas, W., Trilaksana, I.G.N.B., & Pemayun, T.G.O. (2019). Umur memengaruhi volume semen dan motilitas spermatozoa Babi Landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali. Jurnal Veteriner, 20(3), 324–329. https://doi.org/10.19087/jveteriner.2019.20.3.324
Sutriana, A., Hasibuan, M.A.L., Armansyah, T., Siregar, T.N., Panjaitan, B., Sayuti, A., & Aliza, D. (2021). Pemberian Ekstrak Akar Pasak Bumi Meningkatkan Kualitas Spermatozoa Domba Waringin. Jurnal Veteriner, 22(3), 317-323. https://doi.org/10.19087/jveteriner.2021.22.3.317
Tako, A.I., Nalley, W.M., Setyani, N.M.P., & Hine, T.M. (2025). Efek Penambahan Kuning Telur Pada Level yang Berbeda Dalam Pengencer Spermax Terhadap Kualitas Semen Beku Babi Landrace. Jurnal Ilmiah Ilmu-Ilmu Peternakan, 28(2), 199-210. https://doi.org/10.22437/jiiip.v28i2.44806
Waberski, D., Riesenbeck, A., Schulze, M., Weitze, K.F., & Johnson, L., (2019). Application of preserved boar semen for artificial insemination: Past, present and future challenges. Theriogenology, 137, 2-7. https://doi.org/10.1016/j.theriogenology.2019.05.030
Yuan, C., Wang, J., & Lu, W., (2023). Regulation of semen quality by fatty acids in diets, extender, and semen. Frontiers in Veterinary Science, 10, 1119153. https://doi.org/10.3389/fvets.2023.1119153
Published
2026-06-21
How to Cite
Silalahi, P., Panjaitan, P., & Tafonao, I. (2026). PENGENCER SEMEN YANG DIPERKAYA MINYAK IKAN MENJAGA KUALITAS SPERMATOZOA BABI (Sus scrofa domesticus) SELAMA PENYIMPANAN JANGKA PENDEK Fish Oil Enriched Semen Extender Protect Boar (Sus scrofa domesticus) Spermatozoa Quality During Short Term Storage. JURNAL NUKLEUS PETERNAKAN, 13(1), 1-7. https://doi.org/10.35508/nukleus.v13i1.25850
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