PENGARUH LEVEL OPTIMAL EKSTRA DAUN KELOR DALAM TIGA PENGENCER TERHADAP KEBERHASILAN INSEMINASI BUATAN TERNAK BABI SECARA IN VIVO (Effect of optimal Levels Of Moringa Leaf Extract Levels in Three ExtenderOn The Succes Of Artificial Insemination In Pigs...
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Daniel Kurniawan Tulle Dani Tulle(1*)
Universitas Nusa Cendana
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Wilmientje Marlene Nalley(2)
Universitas Nusa Cendana
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Thomas Mata Hine(3)
Universitas Nusa Cendana
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Henderiana Laura Louisa Belli(4)
Universitas Nusa Cendana
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Arnold Elyazar Manu(5)
Universitas Nusa Cendana
(*) Corresponding Author
Abstract
Artificial insemination is a modern livestock breeding method that has been widely applied and proven effective in commercial farming systems. This study aimed to evaluate the success of artificial insemination using three types of semen extenders, namely Tris-egg yolk (T-EY), Citrate-egg yolk (C-EY), and Beltsville Thawing Solution-egg yolk (BTS-EY), supplemented with optimal levels of Moringa oleifera leaf extract (MLE), in order to determine the best extender. A total of 18 sows aged 1–2 years were inseminated using liquid semen. A descriptive method was applied to analyze non-return rate (NRR) and conception rate (CR), while a completely randomized design (CRD) was used for service per conception (S/C). The treatments consisted of P1: artificial insemination using T-EY + 2% MLE, P2: C-EY + 4% MLE, and P3: BTS-EY + 2% MLE. The observed variables were NRR, CR, and S/C. The results showed that the T-EY + 2% MLE extender achieved the highest CR and NRR, both reaching 100%, with an S/C value of 1.00. Meanwhile, the C-EY + 4% MLE and BTS-EY + 2% MLE treatments resulted in CR and NRR values of 83.33%, with an S/C value of 1.16. It can be concluded that the T-EY extender supplemented with 2% *Moringa oleifera* leaf extract was the best extender for liquid semen preservation of Landrace boars in artificial insemination programs.
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References
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Ohin MH, Kune P, dan Kihe JN. 2014. Tampilan kinerja reproduksi pada ternak babi betina. Jurnal Nukleus Peternakan, 1(2): 130-134. https://doi.org/10.35508/nukleus.v1i2.756.
Papituan ML, Nalley WM, dan Hine TM. (2024). Preservasi spermatozoa Babi Landrace dalam tiga jenis pengencer modifikasi. TERNAK TROPIKA: Journal of Tropical Animal Production, 25(1): 1-13. https://doi.org/10.21776/ub.jtapro.2024.025.01.1.
Parera H, dan Lenda V. 2023. Evaluation of motility, viability and abnormality of boar spermatozoa in various modified extenders. Jurnal Ilmiah Peternakan Terpadu, 11(1): 13. https://doi.org/10.23960/jipt.v11i1.p13-33.
Parera H, Ndoen B, Lino Y dan Adoe N. 2018. Tingkat keberhasilan inseminasi buatan pada babi menggunakan semen yang diberi ekstrak Mesocarp borassus abellifer linn yang dipreservasi selama 4 hari pada suhu 13°C. Partner, 23(1): 516. https://doi.org/10.35726/jp.v23i1.294.
Parks JE, dan Graham JK. 1992. Effects of cryopreservation procedures on sperm membranes. Theriogenology, 38, 209-222. https://doi.org/10.1016/0093-691X(92)90231-F.
Perumal P, Srivastava SK, Ghosh SK, dan Baruah KK. 2014. Computer-assisted sperm analysis of freezable and nonfreezable mithun (Bos frontalis) semen. Journal of Animals, 1–6. https://doi.org/10.1155/2014/675031.
Potret SP, Marawali A, Riwu AR, dan Kune P. 2024. Kombinasi sari buah semangka dalam pengencer kuning telur dan larutan Tris terhadap kualitas spermatozoa babi landrace. Animal Agricultura, 2(2): 593-601. https://doi.org/10.23960/jipt.v11i1.p13-33.
Prastiya RA, Suprayogi TW, Debora AE, dan Wijayanti A. 2023. Green tea extract addition into a Tris-based egg yolk extender improves Bali Bull sperm quality. Animal Bioscience, 36(2): 20-9217. https://doi.org/10.5713/ab.22.0184.
Putri LR, dan Sagala NRAY. 2023. Analisis faktor-faktor yang mempengaruhi keberhasilan inseminasi buatan pada sapi. Prosiding Semnas BIO 5, 3(1): 541-548. https://doi.org/10.24036/prosemnasbio/vol3/616.
Putri TD, Siregar TN, Thasmi CN, Melia J, dan Adam M. 2020. Faktor-faktor yang mempengaruhi keberhasilan inseminasi buatan pada sapi di Kabupaten Asahan, Sumatera Utara. Jurnal Ilmiah Peternakan Terpadu, 8(21): 111–119. https://doi.org/10.23960/jipt.v8i3.p111-119.
Saifudin M, Isnaini N, Puspita A, Yekti A, dan Susilawati T. (2018). Tingkat keberhasilan inseminasi buatan menggunakan media pengencer tris aminomethan kuning telur pada Sapi Persilangan Ongole. Jurnal Ternak Tropika, 19(1): 60–65. https://doi.org/10.21776/ub.jtapro.2018.019.01.9.
Shokry DM, Eldaim MA, Badr MR, Khalifa HK, Orabi SH, Hassan AM, dan Dohreig R. 2021. Enhancement impact of Moringa oleifera leaves extract – base extender on cryopreservation and fertilization of Barki ram sperms: Comparative study with vitamin E and selenium combination. Italian Journal of Animal Science, 20(1): 1175-1186. https://doi.org/10.1080/1828051X.2021.1953411.
Toelihere MR. 1993. Inseminasi Buatan Pada Ternak. Angkasa, Bandung.
Baku A, Dethan AA, dan Tahuk PK. 2022. Kualitas semen babi landrace dalam pengencer semen sitrat- kuning telur yang ditambah glukosa dengan konsentrasi berbeda. Journal of Tropical Animal Science and Technology, 4(1): 42-55. https://doi.org/10.32938/jtast.v4i1.1268.
Bebas, W., Buyona, G.L., & Budiasa, M.K. (2016). Penambahan vitamin E pada pengencer BTS terhadap daya hidup dan motilitas spermatozoa Babi Landrace pada penyimpanan 150oC. Buletin Veteriner Udayana, 8(1): 1-7.
Bette Y, dan Nalley WM 2025. Tingkat Keberhasilan Inseminasi Buatan Menggunakan Madu dalam Larutan Tris-Kuning Telur pada Kambing Kacang. Jurnal Ilmiah Peternakan Terpadu, 13(1): 98-108.
Bustani GS, dan Baiee FH. 2021. Semen Extenders: An Evaluative Overview of Preservative Mechanisms of Semen and Semen Extenders. Veterinary World, 14: 1220-1233. https://doi.org/10.14202/vetworld.2021.1220-1233.
Cardoso CEC, Wiltbank MC, dan Sartori R. 2021. Factors that optimize reproductive efficiency in dairy herds with an emphasis on timed artificial insemination programs. Animals, 11, 1-30. https://doi.org/10.3390/ani11020301.
Chun XZ, dan Zeng MY. 2000. Evaluation on sperm quality of freshly ejaculated boar semen during in vitro storage under different temperatures. Theriogenology, 53(7): 1477-1488. https://doi.org/10.1016/S0093-691X(00)00290-9.
Djawapatty DJ, Belli HLL dan Hine TM. 2018. Fertilitas In Vitro dan In Vivo Spermatozoa Babi Landrace pada Pengencer Sitrat Kuning Telur yang Disuplementasi Berbagai Level Fruktosa pada Penyimpanan Suhu 18oC. Jurnal Sain Peternakan Indonesia, 13(1): 43-54. https://doi.org/10.31186/jspi.id.13.1.43-54.
Fafo M, Hine TM, dan Nalley WM. 2016. Pengujian Efektivitas Ekstrak Daun Kelor Dalam Pengencer Sitrat-Kuning Telur Terhadap Kualitas Semen Cair Babi. Jurnal Nukleus Peternakan, 3(2): 184-195. https://doi.org/10.35508/nukleus.v3i2.805.
Garner DL, dan Hafez ESE. 2000. Spermatozoa and Seminal Plasma. Reproduction in Farm Animals, 96-109. https://doi.org/10.1002/9781119265306.ch7.
Gopalakrishnan L, Doriya K dan Santhosh D. 2016. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, 5(2): 49-56. https://doi.org/10.1016/j.fshw.2016.04.001.
Knox RV. 2011. Plenary article the current value of frozen-thawed boar semen for commercial companies the potential for fts to reduce risk of use of fts for gene transfer. Reproduction in Domestic Animals, 46(2): 4-6. https://doi.org/10.1111/j.1439-0531.2011.01822.x.
Kuswati K, Yekti APA, dan Susilawati T 2022. The Relationship of Estrous Character with Conception Rate in Madura Cattle. Jurnal Ilmu - Ilmu Peternakan, 32(3): 407-415. https://doi.org/10.21776/ub.jiip.2022.032.03.12.
Lenda V, Parera H, Foeh ND, dan Sirat MMP. 2025. Pengaruh berbagai pengencer berbasis tris-sitrat-fruktosa terhadap keutuhan membran plasma, fragmentasi DNA sperma, dan keberhasilan inseminasi buatan pada babi. Jurnal Kajian Veteriner, 13(1): 89-97. https://doi.org/10.35508/jkv.v13i1.19526.
Maulana R, Susetya H, dan Prihatno S. 2022. Prevalence and risk factors associated with repeat breeding of beef cattle in Sleman Regency, Indonesia. Veterinary World, 15(4): 870-877. https://doi.org/10.14202/vetworld.2022.870-877.
Mengistu DA, Tezera SD, Birhanie MK, Kolech TA, Mekonen AW, Wassie AT, Endeshaw AG, dan Mekonnen SA. 2026. Assessment of artificial insemination practices, semen quality, and reproductive constraints in dairy farms in Central Gondar Zone, Ethiopia. BMC Veterinary Research, 22(5): 1-13. https://doi.org/10.1186/s12917-025-05161-5.
Ohin MH, Kune P, dan Kihe JN. 2014. Tampilan kinerja reproduksi pada ternak babi betina. Jurnal Nukleus Peternakan, 1(2): 130-134. https://doi.org/10.35508/nukleus.v1i2.756.
Papituan ML, Nalley WM, dan Hine TM. (2024). Preservasi spermatozoa Babi Landrace dalam tiga jenis pengencer modifikasi. TERNAK TROPIKA: Journal of Tropical Animal Production, 25(1): 1-13. https://doi.org/10.21776/ub.jtapro.2024.025.01.1.
Parera H, dan Lenda V. 2023. Evaluation of motility, viability and abnormality of boar spermatozoa in various modified extenders. Jurnal Ilmiah Peternakan Terpadu, 11(1): 13. https://doi.org/10.23960/jipt.v11i1.p13-33.
Parera H, Ndoen B, Lino Y dan Adoe N. 2018. Tingkat keberhasilan inseminasi buatan pada babi menggunakan semen yang diberi ekstrak Mesocarp borassus abellifer linn yang dipreservasi selama 4 hari pada suhu 13°C. Partner, 23(1): 516. https://doi.org/10.35726/jp.v23i1.294.
Parks JE, dan Graham JK. 1992. Effects of cryopreservation procedures on sperm membranes. Theriogenology, 38, 209-222. https://doi.org/10.1016/0093-691X(92)90231-F.
Perumal P, Srivastava SK, Ghosh SK, dan Baruah KK. 2014. Computer-assisted sperm analysis of freezable and nonfreezable mithun (Bos frontalis) semen. Journal of Animals, 1–6. https://doi.org/10.1155/2014/675031.
Potret SP, Marawali A, Riwu AR, dan Kune P. 2024. Kombinasi sari buah semangka dalam pengencer kuning telur dan larutan Tris terhadap kualitas spermatozoa babi landrace. Animal Agricultura, 2(2): 593-601. https://doi.org/10.23960/jipt.v11i1.p13-33.
Prastiya RA, Suprayogi TW, Debora AE, dan Wijayanti A. 2023. Green tea extract addition into a Tris-based egg yolk extender improves Bali Bull sperm quality. Animal Bioscience, 36(2): 20-9217. https://doi.org/10.5713/ab.22.0184.
Putri LR, dan Sagala NRAY. 2023. Analisis faktor-faktor yang mempengaruhi keberhasilan inseminasi buatan pada sapi. Prosiding Semnas BIO 5, 3(1): 541-548. https://doi.org/10.24036/prosemnasbio/vol3/616.
Putri TD, Siregar TN, Thasmi CN, Melia J, dan Adam M. 2020. Faktor-faktor yang mempengaruhi keberhasilan inseminasi buatan pada sapi di Kabupaten Asahan, Sumatera Utara. Jurnal Ilmiah Peternakan Terpadu, 8(21): 111–119. https://doi.org/10.23960/jipt.v8i3.p111-119.
Saifudin M, Isnaini N, Puspita A, Yekti A, dan Susilawati T. (2018). Tingkat keberhasilan inseminasi buatan menggunakan media pengencer tris aminomethan kuning telur pada Sapi Persilangan Ongole. Jurnal Ternak Tropika, 19(1): 60–65. https://doi.org/10.21776/ub.jtapro.2018.019.01.9.
Shokry DM, Eldaim MA, Badr MR, Khalifa HK, Orabi SH, Hassan AM, dan Dohreig R. 2021. Enhancement impact of Moringa oleifera leaves extract – base extender on cryopreservation and fertilization of Barki ram sperms: Comparative study with vitamin E and selenium combination. Italian Journal of Animal Science, 20(1): 1175-1186. https://doi.org/10.1080/1828051X.2021.1953411.
Toelihere MR. 1993. Inseminasi Buatan Pada Ternak. Angkasa, Bandung.
Published
2026-06-21
How to Cite
Tulle, D. K. T., Nalley, W., Hine, T., Belli, H., & Manu, A. (2026). PENGARUH LEVEL OPTIMAL EKSTRA DAUN KELOR DALAM TIGA PENGENCER TERHADAP KEBERHASILAN INSEMINASI BUATAN TERNAK BABI SECARA IN VIVO (Effect of optimal Levels Of Moringa Leaf Extract Levels in Three ExtenderOn The Succes Of Artificial Insemination In Pigs. JURNAL NUKLEUS PETERNAKAN, 13(1), 25-32. https://doi.org/10.35508/nukleus.v13i1.27679
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