PENGARUH PENAMBAHAN SARI WORTEL DALAM PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING BLIGON (The effect of carrot juice supplementation in citrate - egg yolk extender on spermatozoa quality of bligon goat)
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Maria Epifania Barek(1)
universitas nusa cendana
-
kirenius uly(2)
-
Thomas Mata Hine(3*)
universitas nusa cendana
-
Wilmientje Marlene Nalley(4)
universitas nusa cendana
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Henderiana L.L. Belli(5)
universitas nusa cendana
(*) Corresponding Author
Keywords:
citrate egg yolk, carrot juice, spermatozoa, bligon goat
Abstract
This study aims to determine the effect of adding various doses of carrot juice into citrate-egg yolk diluents (C-EY) on bligon buck semen quality. Semen was collected twice a week using an artificial vaginal method of three buck with normal body condition and reproductive organs. Semen was 70% of motility, konsentration ≥1000x106mL and abnormality ≤ 15 diluted with C-EY added with carrot juice at a concentrations: 0% (CJ-0), 10% (CJ-10), 12.5% (CJ-12,5), 15% (CJ-15), 17.5% (CJ-17,5), and 20% (CJ-20), and stored in a refrigerator at 3-5o C. The quality of spermatozoa was evaluated every 24 hours. The Variables measured were spermatozoa motility, viability, and abnormalities. The results showed that spermatozoa preserved by C-EY supplemented by 17.5% carrot juice (CJ-17.5) had a higher quality (P<0.05) compared to the other four treatments, with motility (49.04 ± 1.0%), viability (55.70 ± 1.22%), dan abnormalities (4.57 ± 0.88 %) on the 4th day of storage. This study concludes that 17.5% carrot juice into citrate eggyolk extender improves the bligon buck semen
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References
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Partama IBG. 2000. Kebutuhan energi dan protein kambing Peranakan Etawah calon pejantan. Disertasi.
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Parera FZ, Prihatiny DF, Souhoka, Rizal M. 2009. pemanfaatan sari wortel sebagai pengencer alternative spermatozoa epididimis sapi Bali. Indonesia Tropical Animal Agriculture. 34(1):50-56.
Rizal M. 2008. Peningkatan kualitas spermatozoa epididimis kerbau belang yang dikriopreservasi dengan beberapa konsentrasi sukrosa. Jurnal Ilmu Ternak dan Veteriner 8(4):188-183.
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Suryohudoyo P. 2000. Oksidan, antioksidan, dan radikal bebas. Dalam: Kapita Selekta Ilmu Kedokteran Molekuler. Suryohudoyo, P. CV Sagung Seto, Jakarta. hlm. 31-47.
Salisbury B, Ross CW. 1995. Fisiologi Tumbuhan. Jilid 1. Edisi IV. ITB, Bandung.
Suyadi A, Rachmawati N, Iswanto. 2012. Pengaruh α-Tocopherol yang berbeda dalam pengencer dasar tris aminomethane kuning telur terhadap kualitas semen kambing Boer yang disimpan pada suhu 50c. Jurnal Ilmiah Ilmu-ilmu Peternakan. 22(3): 1-8.
Tambing SN, Toelihere MR, Yusuf TL, Sutama IK. 2000. Pengaruh gliserol dalam pengencer tris terhadap kualitas semen beku kambing Etawah. J. Ilmu Ternak Vet. 5(2): 1-8.
Tambing SN, Sutama IK, Arifiantini RI. 2003. Efektevitas berbagai konsentrasi laktosa dalam pengencer tris terhadap viabilitas semen cair kambing Saanen. JITV. 8(2): 84-90
Thamburaj S, Singh N. 2005. Textbook of Vegetables, Tuber Crops And Spices. Indian Council of Agriculture Research. New Delhi.
Tambing NS, Gazali M, Purwantara B. 2001. Pemberdayaan teknologi inseminasi buatan pada ternak kambing. Wartazoa. 11(1): 1 - 6.
Yulnawati MA, Setiadi, Herdis. 2005. Pemanfaatan sari buah melon dan sari wortel sebagai media pengencer alternatif semen cair domba Garut. Protein 1(2): 151-160.
Yendraliza, Musyrifin M, Elviriadi, Zumarni, Rodiallah M. 2018. Viabilitasspermatozoa sapi bali menggunakan pengencer andromed dengan penambahan konsentrasi sari wortel yang berbeda. Jurnal Ilmu dan Teknologi Peternakan Tropis. 6(2): 239-245.
Yotov SI, Fasulkovand N, Vassilev. 2011. Effect of ejaculation frequency onspermatozoa survival in diluted semen from pleven blackhead rams. Turk. J. Vet. Anim. Sci. 35(2): 117-122.
Arifiantini RI, Purwantara B. 2010. Motility and viability of Friesian Holstein spermatozoa in three different extender stored at 5oC. Jurnal of the Indonesian Tropical Animal Agriculture. 35(4): 222-226.
Agarwal A, Sekhon LH. 2010. The role of antioxidant therapy in the treatment of male infertility. Human Fertility. 13(4): 217-25.
Babic I, Amiot MJ, Ngugen The C, Aubert S. 1993. Changes in phenolic content in fresh, ready-to-use and shredded carrots during storage. Journal of Food Science. 58(2): 351- 356.
Basbeth AH, Dilaga WS, Purnomoadi A. 2015. Hubungan antara ukuran- ukuran tubuh terhadap bobot bada kambing bligon jantan umur muda di Kabupaten Kendal, Jawa Tengah. Animal Agriculture. Journal. 4 (1): 35 - 40.
Block G. 1994. Nutrient source of pro-vitamin a carotenoids in American diet. Am J Epidemiol. 13(9): 290-293.
Bintara S. 2011. Rasio spermatozoa X : Y dan kualitas sperma kambing Kacang dan Peranakan Ettawa. J. Sains Peternakan. Vol. 9(2): 65-71.
Fraga CG, Motchnik PA, Shigenaga MK, Helbock HJ, Jacobs RA, Ames BN. 1991. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Medical Sciences. 88(24): 11003-11006.
Fuller GM, Shields D. 1998. Molecular basis of medical cell biology. Prentice Hall International, Inc. USA.
Gopalan C, Ramasastry BV, Balasubramanian SC. 1991. Nutritive value of Indian foods. National Institute of Nutrition. Hyderabad.
Ganong WF. 2001. Buku Ajar Fisiologi Kedokteran. Buku Kedokteran EGC, Jakarta.
Garner DL, Hafez ESE. 2000. Spermatozoa and seminal plasma. In: Hafez ESE and
Hafez B (Eds). Reproduction In Farm Animals. 7th Ed. Lippincott Williams and Wilkins, Philadelphia. pp : 96-109.
Hansen SL, Purup S, Christensen LP. 2003. Bioactivity of falcarinol and the influence of processing and storage on its content in carrots (Daucus carota L). Journal of Science and Food Agriculture. 83(10):1010-1017.
Hashimoto T, Nagayama T. 2004. Chemical composition of ready-to-eat fresh carrot. J. Food Hyg Soc Japan. 39(1):324-328.
Hammersted R. 1993. Maintenance of bioenergetic in sperm and prevention of lipid peroxidation. A review of the effect on design of storage preservation systems. Australia. Reprod Fertil Dev 5(2): 675-690.
Herdis, Yulnawati, Setiadi MA. 2003. Pemanfaatan buah wortel sebagai media pengencer semen cair alternatif spermatozoa domba Garut. Jurnal Sains dan Teknologi Indonesia. 5(1): 126-131.
Halliwell B, Gutteridge JMC. 1990. Free radicals in biology and medicine. Clarendon Press. Oxford.
Holland B, Unwin JD, Buss DH. 1991. Vegetables, herbs and spices. Fifth supplement to mc cance and widdowson’s. London.
Hartono M. 2008. Optimalisasi penambahan vitamin c dalam pengencer sitrat kuning telur untuk mempertahankan kualitas semen kambing boer. J Indon Trop Anim Agric. 33(1):11-19.
Hastono, Adiati U, Praharani L. 2013. Libido, kemampuan kawin dan kualitas sperma kambing dari tiga bangsa. Seminar Nasional Teknologi Peternakan dan Veteriner. Balai Penelitian Ternak.
Ihsan NM. 2009. Bioteknologi Reproduksi Ternak. Universitas Brawijaya. Malang.
Johnson LA, Weitze KF, Fiser P, Maxwell WMC. 2000. Storage of Boar Semen. J. Anim. Sci. 62(1): 143-172.
Kidmose U, Hansen SL, Christensen LP, Edelenbos M, Larsen M, Norback R. 2004. Effects of genotypes, root size, storage and processing on bioactive compounds in organically grown carrots (Daucus carota L). Journal of Food Science. 69(1):388-394.
Kartasudjana R. 2001. Teknik Inseminasi Buatan. Jakarta: Departemen Pendidikan Nasional.
Kostaman T, Sutama IK. 2006. Studi motilitas dan daya hidup spermatozoa kambing Boer pada pengencer tris-sitrat-fruktosa. J. Sain Veteriner. 24(1):58-63.
Lenzi A, Gandini L, Lombardo F, Picardo M, Maresca V, Panfili E, Tramer F, Boitani C, Dondero F. 2002. Polyunsaturated fatty acids of germ cell membranes, glutathione and glutathione-dependent enzyme-PHGPx: from basic to clinic. Contraception. 65(1): 301-304.
Lestari TPS, Ihsan MN, Isnaini N 2014. Pengaruh waktu simpan semen segar dengan pengencer andromed pada suhu ruang terhadap kualitas semen kambing Boer. Jurnal Ternak Tropika. 15(1):43-50).
Nugroho Y, Susilawati T, Wahjuningsih S. 2015. Kualitas semen sapi Limousin selama pendinginan menggunakan pengencer cep-2 dengan penambahan berbagai konsentrasi kuning telur dan sari buah jambu biji (Psidium guajava). Jurnal. Ternak Tropika. 15(1):31-42.
Partama IBG. 2000. Kebutuhan energi dan protein kambing Peranakan Etawah calon pejantan. Disertasi.
Program Pascasarjana. Institut Pertanian Bogor, Bogor.
Parera FZ, Prihatiny DF, Souhoka, Rizal M. 2009. pemanfaatan sari wortel sebagai pengencer alternative spermatozoa epididimis sapi Bali. Indonesia Tropical Animal Agriculture. 34(1):50-56.
Rizal M. 2008. Peningkatan kualitas spermatozoa epididimis kerbau belang yang dikriopreservasi dengan beberapa konsentrasi sukrosa. Jurnal Ilmu Ternak dan Veteriner 8(4):188-183.
Rubatzky E, Yamaguchi M. 1997. World Vegetables: Principles, Production, and Nutritive Value. A Division of International Thomson Publishing Inc. 320 pp.
Suryohudoyo P. 2000. Oksidan, antioksidan, dan radikal bebas. Dalam: Kapita Selekta Ilmu Kedokteran Molekuler. Suryohudoyo, P. CV Sagung Seto, Jakarta. hlm. 31-47.
Salisbury B, Ross CW. 1995. Fisiologi Tumbuhan. Jilid 1. Edisi IV. ITB, Bandung.
Suyadi A, Rachmawati N, Iswanto. 2012. Pengaruh α-Tocopherol yang berbeda dalam pengencer dasar tris aminomethane kuning telur terhadap kualitas semen kambing Boer yang disimpan pada suhu 50c. Jurnal Ilmiah Ilmu-ilmu Peternakan. 22(3): 1-8.
Tambing SN, Toelihere MR, Yusuf TL, Sutama IK. 2000. Pengaruh gliserol dalam pengencer tris terhadap kualitas semen beku kambing Etawah. J. Ilmu Ternak Vet. 5(2): 1-8.
Tambing SN, Sutama IK, Arifiantini RI. 2003. Efektevitas berbagai konsentrasi laktosa dalam pengencer tris terhadap viabilitas semen cair kambing Saanen. JITV. 8(2): 84-90
Thamburaj S, Singh N. 2005. Textbook of Vegetables, Tuber Crops And Spices. Indian Council of Agriculture Research. New Delhi.
Tambing NS, Gazali M, Purwantara B. 2001. Pemberdayaan teknologi inseminasi buatan pada ternak kambing. Wartazoa. 11(1): 1 - 6.
Yulnawati MA, Setiadi, Herdis. 2005. Pemanfaatan sari buah melon dan sari wortel sebagai media pengencer alternatif semen cair domba Garut. Protein 1(2): 151-160.
Yendraliza, Musyrifin M, Elviriadi, Zumarni, Rodiallah M. 2018. Viabilitasspermatozoa sapi bali menggunakan pengencer andromed dengan penambahan konsentrasi sari wortel yang berbeda. Jurnal Ilmu dan Teknologi Peternakan Tropis. 6(2): 239-245.
Yotov SI, Fasulkovand N, Vassilev. 2011. Effect of ejaculation frequency onspermatozoa survival in diluted semen from pleven blackhead rams. Turk. J. Vet. Anim. Sci. 35(2): 117-122.
Published
2020-12-17
How to Cite
Barek, M., uly, kirenius, Hine, T., Nalley, W., & Belli, H. (2020). PENGARUH PENAMBAHAN SARI WORTEL DALAM PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING BLIGON (The effect of carrot juice supplementation in citrate - egg yolk extender on spermatozoa quality of bligon goat). JURNAL NUKLEUS PETERNAKAN, 7(2), 109 - 117. https://doi.org/10.35508/nukleus.v7i2.3152
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