KUALITAS SPERMATOZOA KAMBING BLIGON DALAM PENGENCER TRIS-KUNING TELUR DENGAN PENAMBAHAN BERBAGAI LEVEL EKSTRAK KULIT BUAH NAGA (Spermatozoa quality of bligon goat in tris - egg yolk diluent added with various levels of dragon fruit peel extract)

  • Maria F. T. Leyn(1)
    universitas nusa cendana
  • Henderiana Belli(2)
    Universitas Nusa Cendana
  • Wilmientje Marlene Nalley(3)
  • Petrus Kune(4)
    Universitas Nusa Cendana
  • Thomas Mata Hine(5*)
    universitas nusa cendana
  • (*) Corresponding Author
Keywords: Tris, egg yolk, dragon fruit extract, spermatozoa, bligon goat

Abstract

The purpose of this study was to examine the effect of adding various concentrations of dragon fruit peel extract (DFPE) in Tris-egg yolk diluent (TEY) on the quality of bligon goat spermatozoa. Semen was collected twice a week using the artificial vaginal method from three year old bligon male goats with normal body conditions and reproductive organs. After the macroscopic and microscopic evaluation, good quality semen with a sperm motility of > 70%, a concentration of > 800 x 106 mL-1, and abnormality of < 15 %, was diluted with TEY + DFPE at a concentration of 0% (P0), 2% (P1), 4% (P2), 6% (P3), 8% (P4), dan 10% (P5). The semen which still had a motility ≥ 70% was then stored at a temperature of 3-5oC. Evaluation of the quality of spermatozoa  was carried out every 24 hours until the motility decrease to 40%. The results showed that from day 1 to day 5 of preservation, spermatozoa preserved in TEY + 6% DFPE diluent (P3) had higher motility and viability (P<0,05) than other treatments. The abnormalities of spermatozoa until the 5 day of preservation were below 6%, but showed a significant difference (P<0,05) between treatments that received DFPE suplemention with control (P0). It was concluded that the addition of DFPE in TEY up to 6% could reduce the decrease in sperm quality of bligon goats during in vitro preservation.

Downloads

Download data is not yet available.

References

Abdullah A, Abdullah N. 2011. Quality characteristics and acceptability of three types of pitaya fruit in a consumer acceptance test. Journal of Tourism Hospitality & Culinary Arts 3(1): 89-98.
Arifiantini RI, Yusuf TL. 2006. Keberhasilan menggunakan tiga bahan pengencer dalam dua jenis kemasan pada proses pembekuan semen sapi frisien holstein. Majalah Ilmiah Peternakan 9(3): 89-93.
Aslam, Dasrul HA, Rosmaidar. 2014. Pengaruh penambahan vitamin C dalam pengencer andromed terhadap persentase motilitas dan membrane plasma utuh spermatozoa sapi Aceh setelah pembekuan. Jurnal Medika Veterinaria 8(1): 20-26.
Azzahra FY, Setiatin ET, Samsudewa D. 2016. Evaluasi motilitas dan presentase hidup semen segar sapi PO kebumen pejantan muda. Jurnal Sains Peternakan Indonesia 2: 99-107.
Bintara S. 2011. Rasio X:Y dan kualitas sperma pada kambing kacang dan peranakan ettawa. Sains Peternakan 9(2): 65-71.
Fitri NL, Susetyarini RE, Waluyo L. 2016. The effect of ciplukan (Physalis angulata L) fruit extract on SGPT and SGOT levels against white male mice (Mus Musculus) hyperglycemia induced by alloxan as biology learninf reso urces. Jurnal Pendidikan Biologi Indonesia 2(2): 180-187.
Foote RH. 1980. Artificial insemination. In: E.S.E. Hafez (Ed.) Reproduction in Farm Animals 4th Ed. Lea and Febiger, Philadelphia.
Garner DL, Hafez ESE. 2000. Spermatozoa and Seminal Plasma. In Farm Animals. Edited by E.S.E. Hafez.7th edition. Lippincott Williams and Wilkins. Maryland, USA.
Hafez ESE. 2000. Reproduction In Farm Animal. 7th ed., Lippincott Williams and Wilkins, Philadelphia.
Hidayati N, Arifiantini RI, Sajuthi D. 2015. Preservasi semen kambing peranakan ettawa dalam pengencer tris dan sitrat kuning telur dengan penambahan sodium dodecyl sulphate. Jurnal Veteriner 16(3): 334-342.
Ihsan MN. 2008. Upaya peningkatan konsentrasi spermatozoa hasil pemisahan dengan sentrifugasi gradient densitas percoll pada sapi friesien holstein (FH). Disertasi. Program Pascasarjana, Universitas Brawijaya, Malang.
Kartusudjana R. 2001. Ciri-ciri atau Tanda Keabnormalitasan pada Semen Kambing Peranakan Ettawa (PE). Departemen Pendidikan Nasional. Jakarta.
Khaki A, Fathiazad F, Nouri M, Amir AK. 2011. Effect of Ocimum basilicum on apoptosis in testis if rats after exposure to electromagnetic field. Afr J Pharm Parmacol. 5(1): 1534-1537.
Kusumawati DE, Utomo KN, Krisnaningsih AJN, Syam R. 2017. Kualitas semen kambing Kacang dengan lama simpan yang berbeda pada suhu ruang menggunakan pengencer tris aminomethan kuning telur. In Seminar Nasional Hasil Penelitian. JITRO 4(2): 42-51.
Lubis TM, Dasrul, Thasmi CN, Akbar T. 2013. Efektivitas penambahan vitamin C dalam pengencer susu skim kuning telur terhadap kualitas spermatozoa kambing boer setelah penyimpanan dingin. Jurnal S. Pertanian 3(1): 347-361.
Manuhuruk FM. 2016. Efektivitas penambahan ekstrak kulit buah naga merah (Hylocereus polyrhizus) sebagai pewarna, antioksidan, dan antimikroba pada sosis daging sapi selama penyimpanan dingin. Tesis. Institut Pertanian Bogor.
Maxwell WMC, Watson PF. 1996. Recent progress in the preservation of ram semen. J. Anim. Reprod. Sci. 42(10): 55-65.
Munawaroh. 2014. Penambahan ekstrak buah naga (Hylocereus ondotus) untuk pengawetan spermatozoa kambing peranakan boer pada suhu 5oC. Scripta Biologica 4(4): 223-247.
Nalley WM, Handarini R, Purwantara B. 2007. Viabilitas spermatozoa rusa Timor (Cervus timorensis) di dalam pengencer tris kuning telur dengan sumber karbohidrat yang berbeda yang disimpan pada suhu ruang. Jurnal Ilmu Ternak dan Veteriner 12(4): 311-317.
Noor MI, Yufita EY, Zulfalina. 2016. Identifikasi kandungan ekstrak kulit buah naga merah menggunakan fourier transform infrared (FTIR) dan phytochemistry. Journal of Aceh Physics Society 5(1):14-16.
Nurliyana R, Zahir IS, Suleiman KM, Aisyah MR, Rahim KK. 2010. Antioxidant study of pulpl and peels of dragon fruids: a comparave study. International Food Research Journal 17(1):367-375.
Partama IBG. 2000. Kebutuhan energi dan protein kambing peranakan ettawah calon pejantan. Disertasi. Program Pascasarjana, Institut Pertanian Bogor. Bogor.
Partodihardjo S. 2002. Ilmu Reproduksi Hewan. Penerbit Mutiara Sumber Widya. Jakarta.
Purdy PH. 2006. A review on goat sperm cryopreservation. Small Rumin Research 63(3): 215-225.
Putranti OD, Kustono, Ismaya. 2010. Pengaruh penambahan crudetannin pada sperma cair kambing peranakan ettawa yang disimpan selama 14 hari terhadap viabilitas spermatozoa. Buletin Peternakan 34(1): 1-7.
Rizal M, Herdis M. 2010. Peranan antioksidan dalam meningkatkan kualitas semen beku. Jurnal Wartazoa 20(3):139-146.
Setiadi B, Sutama IK, Situmorang P, Supriyati, Adiati U, Budiarsa IGM, Kostaman T, Maulana, Mulyawan. 2000. Evaluasi karakteristik semen kambing calon bibit. Laporan Bagian Proyek Rekayasa Teknologi Peternakan ARMP-II: 74-87. Balai Penelitian Ternak.
Shukla SN, Singh BB, Tomarand NS, Misra BS. 1992. Factors affecting spermatozoa motility in preserved semen. Indian Vet. J. 69 (2): 856-857.
Siregar TN, Hamdan. 2007. Teknologi Reproduksi Pada Ternak. CV. Mita Mulia, Banda Aceh.
Siswandoko B, Zaenab S, Husamah. 2017. Penambahan ekstrak kulit buah naga ke dalam pengencer tris kuning telur untuk meningkatkan kualitas semen beku kambing peranakan ettawa. Jurnal Scripta biologica 4(4): 247-251.
Susilawati T. 2011. Spermatology. UB Press. Malang.
Sutiyono, Riyadi S, Kismiati S. 2006. Fertilisasi dan daya tetas telur dari ayam petelur hasil inseminasi buatan menggunakan semen ayam kampong yang diencerkan dengan bahan berbeda. J. Pengembangan Peternakan Tropis. 31 (1): 36-40.
Suwarso. 1999. Peranan ranifosa dalam pengencer tris sitrat kuning telur terhadap kualitas semen beku kambing peranaakn ettawah. Thesis. Program Pascasarjana, Institut Pertanian Bogor. Bogor.
Tambing SN, Toelihere MR, Yusuf TL, Sutama IK. 2000. Pengaruh gliserol dalam pengencer tris terhadap kualitas semen beku kambing peranakan ettawa. JITV 5(1): 84-91.
Toelihere MR. 1993. Inseminasi Buatan Pada Ternak. Angkasa. Bandung.
Varasofiari LN, Setiatin ET, Sutopo. 2013. Evaluasi semen segar sapi jawa brebes berdasarkan lama waktu penyiapan. Animal Agriculture 2(1): 201-208.
Wulandari R, Budiyanta MAK, Waluyo L. 2016. The influence of various concentration of red roses (Rosa damascene mill) flower extract to anthocyanin color stability jelly as biology learning source. Jurnal Pendidikan Biologi Indonesia 2(1): 40-48.
Yotov S, Fasulkov I, Vassilev N. 2011. Effect of ejaculation frequency on spermatozoa survival in diluted from pleven blackhead rams. Turk. J.Vet. Anim. Sci. 2(2): 117-122.
Yustendi D. 2013. Penambahan tepung daun katuk (Saurupus androgynus L. merr) dalam ransum kambing jantan peranakan ettawa terhadap konsumsi pakan, pertambahan berat badan, lingkar scrotum dan kualitas spermatozoa. Thesis. Program Pascasarjana. Universitas Syiah Kuala, Banda Aceh.
Yusuf TL, Arifiantini RI, Rahmiwati N. 2005. Daya tahan semen cair kambing peranakan ettawa dalam pengenceran kuning telur dengan kemasan dan konsentrasi spermatozoa yang berbeda. J. Indon. Trop. Anim. Agric. 30(4): 217-223.
Zaniboni L, Rizzi R and Cerolini S. 2006. Combined effect of DHA and

PlumX Metrics

Published
2021-06-21
How to Cite
Leyn, M., Belli, H., Nalley, W., Kune, P., & Hine, T. (2021). KUALITAS SPERMATOZOA KAMBING BLIGON DALAM PENGENCER TRIS-KUNING TELUR DENGAN PENAMBAHAN BERBAGAI LEVEL EKSTRAK KULIT BUAH NAGA (Spermatozoa quality of bligon goat in tris - egg yolk diluent added with various levels of dragon fruit peel extract). JURNAL NUKLEUS PETERNAKAN, 8(1), 23-32. https://doi.org/10.35508/nukleus.v8i1.4230
Section
Articles

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.