PENGARUH PERBEDAAN WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM MODIFIKASI DENGAN AIR BUAH LONTAR (Effect of different equilibration time on the quality of landrace boar frozen semen on modification diluent....
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Maria G. Mega(1*)
Universitas Nusa Cendana
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Wilmientje M. Nalley(2)
Universitas Nusa Cendana
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Aloysius Marawali(3)
Universitas Nusa Cendana
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Henederiana L. L. Belli(4)
Universitas Nusa Cendana
(*) Corresponding Author
Abstract
An experiment was carried out to recognize the effect of long equilibration time on the quality of spermatozoa and to obtain optimal equilibration time in the process cryopreservation of landrace pig semen. Semen was obtained from a landrace boar aged 2-3 years using glove hand method. The semen was than evaluated macroscopically and microscopically, good quality semen was diluted in a basic diluent, than kept for two hours at a temperature of 27-28oC. The semen were then centrifuged at 2000 rpm for 15 minutes, the supernatant was discarced and the pellets was re-diluted into basic diluent with a ratio of 1:1. Then, semen was diluted in treatment diluent with modified durasperm + 6% lactose + 4% glycerol with a concentration of 500 x 106/0.5 mL. Furthermore, the semen was packaged in 0.5 ml straws, then equilibrated at a temperature of 3-5oC appropriate to treatment, with 1 hour equilibration (T1), 2 hours equilibration (T2) and 3 hours equilibration (T3). After equilibration, the straws was frozen horizontally 5 cm above the liquid N2 vapor for 10 minutes at a temperature of ± -110oC and then stored in a liquid N2 container (-196oC). To test frozen semen, it was done by stored frozen semen at 37oC for 30 seconds. The results showed that the highest quality of spermatozoa (P <0.05) was found in T2 and T3 when compared to T1 (P> 0.05), with the motility value was T2: 28.13 ± 2.39%; T3: 25.00 ± 4.08%; and T1 18,15±3,23%. It can be concluded that 2 hours and 3 hours equilibration time was able to maintain the quality of frozen semen landrace boars and the statistically was not significant.
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References
Ax RL, Dally M, Didion BA, Lenz RW, Love CC, Hafes B. Bellin, ME. 2000. Semen Evaluation. In: Reprod in Farm Anim. Hafes B. Hafes ESE (Ed). 7th ed. Pp. 365–389.
Azizah, Arifiantini R. 2009. Kualitas semen beku kuda pada pengencer susu skim dengan konsentrasi gliserol yang berbeda. Jurnal of Veteriner 10(2): 63-70.
Banamtuan AN, Nalley WM, Hine TM. 2021. Kualitas semen cair babi duroc dalam pengencer durasperm yang disuplementasi air buah lontar dan sari tebu. Jurnal Sain Peternakan Indonesia 16(1): 41-48.
Bearden HJ, Fuquay JW, Willard S. 2004. Applied Animal Reproduction, 6th Eddition. Pearson Education, Inc., Upper Saddle River, New Jersy. Pp: 1-456.
Bebas W, Gorda W. 2020. Kadar krioprotektan gliserol dan dimethylsulfoxide terbaik pada pengencer astaxanthin fosfat kuning telur bebek terhadap kualitas semen beku babi. Jurnal Veteriner 21(36): 115-123.
Febriani GD, Hamdan, Melia J. 2014. Pengaruh waktu ekuilibrasi terhadap kualitas semen kerbau lumpur (Bubalus bubalis) setelah thawing. Jurnal Medika Veterinaria 8(1): 1-4.
Foeh NDFK, Gaina CD, Titong AP, Buta CA, Bei MS. 2019. Daya tahan spermatozoa semen cair babi landrace pada metode penyimpanan berbeda. Jurnal Kajian Veterine 7(1): 47-52.
Foeh, NDFK, Arifiantini RI, Yusuf, TL. 2017. The quality of boar frozen semen diluted in BTS® and MII® with different cryoprotectant supplemented with sodium dodecyl sulphate. Jurnal Kedokteran Hewan 11(1): 6-10.
Gadea J. 2003. Review: semen extenders used in the artificial inseminarion of swine. Spanish Journal of Agricultural Research 1(2): 17-27.
Gao D, Critser JK. 2000. Mechanisms of cryoinjury in living cells. ILAR Journal 41(4): 187-196.
Garner DL, Hafez ESE. 2000. Spermatozoa and seminal plasma. In :Hafez B. Hafez ESE (Editor) Reproduction in Farm Animals. 7th Ed. Philadelphia (US). Lippincott E Williams and Wilkins. pp. 96–109.
Herdiawan I. 2004. Pengaruh laju penurunan suhu dan jenis pengencer terhadap kualitas semen beku domba priangan. Jurnal Ilmu Ternak Dan Veteriner 9(2): 98-107.
Herdis. 2015. Daya motil dan keutuhan membran plasma spermatozoa domba garut (Ovise aries) pada penambahan kolesterol dalam pengencer Tris kuning telur. JSTI. 17(1): 16-23.
Herdis, Rizal M, Boediono A, Arifiantini RI, Aku AS, Yulnawati. 2005. Optimasi kualitas semen beku domba garut melalui penambahan trehalosa ke dalam pengencer kuning telur. Journal of the Indonesian Tropical Animal Agriculture 30(4): 229-236.
Hu J-H, Li Q-W, Li G, Jiang Z-L, Bu S, Yang H, Wang L-Q. 2009. The cryoprotective effect of trehalose supplementation on boar spermatozoa quality. Animal Reproduction Science 112(1-2): 107-118.
Jadi ML, Supit MA, Kusumaningrum D, Angi AH. 2015. Evaluasi kualitas semen beku akibat perbedaan metode lama equilibrasi dan lama penurunan suhu selama prosesing semen. Partner 15(2): 170-177.
Johnson, L. A., Weitze, K. F., Fiser, P., & Maxwell, W. M. C. (2000). Storage of boar semen. Animal Reproduction Science 62(1-3): 143-172.
Kaka A. 2020. Karakteristik dan daya fertilitas spermatozoa babi peranakan landrace. Jurnal Peternakan Indonesia 22(3): 277-283.
Kartina. 2014. Pengaruh lama ekuilibrasi terhadap daya hidup spermatozoa kambing peranakan etawa (PE). Skripsi. Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Hasanuddi, Makassar.
Knox RV. 2011. Semen Processing, Extending And Storage For Artificial Insemination In Swine. In Departemen of Anumal Science. University of Illinois publications. (pp. 1–7).
Lubis TM, Dasrul, Thasmi CN, Akbar T. 2013. Spermatozoa after cold storage efektifitas penambahan vitamin C dalam pengencer susu skim kuning telur terhadap kualitas spermatozoa kambing boer setelah penyimpanan dingin. Jurnal Sains Pertanian 3(1): 347-361.
Morel GCGD. 1999. Artificial Insemination. Cabi Publishing. Wallingford. United Kingdom. (pp. 1–416).
Passarelli MDS, Pavaneli APP, Ravagnani GM, Martins MP, Pedrosa AC, Martins SMMK, de Alcântra RNR, Rigo VHB, Yasui G, Yeste M. 2020. Effects of different equilibration times at 5° C on boar sperm cryotolerance. Animal Reproduction Science 219(10): 106-547.
Sangma TFM, Ahmed K, Choudhury MD, Galib ZU, Ahmed N, Das A. 2020. Characteristics of fresh crossbred hampshire boar semen. Haryana Veteriner 59(1): 98-101.
Saputra DJ, Ihsan MN, Isnaini N. 2017. Korelasi antara lingkar skrotum dengan volume semen, konsentrasi dan motilitas spermatozoa pejantan sapi bali. Jurnal Ternak Tropika: 18(2): 59-68.
Solihati N, Kune P. 2009. Pengaruh jenis pengencer terhadap motilitas dan daya tahan hidup spermatozoa semen cair sapi simmental. Jurnal Fakultas Peternakan Universitas Padjadjaran, Bandung.
Suherlan NE, Soeparna, Hidajat K. 2015. Pengaruh penambahan berbagai tingkat DMF (Dimethylformamide ) sebagai agen krioprotektan terhadap keutuhan membran plasma dan recovery rate semen beku domba lokal. Jurnal Ilmu-IImu Peternakan 4(4): 1-12.
Suryadi. 2016. Daya tahan semen cair babi dalam pengencer BTS yang disuplementasi kuning telur pada suhu penyimpanan berbeda. Skripsi. Departemen Ilmu Produksi danTeknologi. Peternakan-Fakultas Peternakan-Institusi Pertanian Bogor.
Tambing SN, Sutama IK, Arifiantini RI. 2003. Efektivitas berbagai konsentrasi laktosa dalam pengencer tris terhadap viabilitas semen cair kambing saanen. Jurnal Ilmu Ternak dan Veteriner 8(2): 84-90.
Vishwanath R, Shannon P. 2000. Storage of bovine semen in liquid and frozen state. Animal Reproduction Science 62(1-3): 23-53.
Watson PF. 2000. The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science 60: 481-492.
White IG. 1993. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reproduction, Fertility and Development 5(6): 639-658.
Yusuf TL, Arifiantini RI, Dapawole RR, Nalley WM. 2017. Kualitas semen beku babi dalam pengencer Komersial yang disuplementasi dengan trehalosa. Jurnal Veteriner 18(1): 69-75.
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