PENGARUH LAMA EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS-MODIFIKASI DENGAN PENAMBAHAN KRIOPROTEKTAN (Effect of equilibration time on frozen sement quality of the duroc pig in tris diluents modification With.....

  • Biqshierz V.W Koelima Universitas Nusa Cendana
  • Henderiana L.L. Belli Universitas Nusa Cendana
  • Johny N. Kihe Universitas Nusa Cendana
  • Wilmientje M. Nalley Universitas Nusa Cendana
Keywords: equilibration, duroc pig, frozen semen

Abstract

The purpose of this study was to test the influence of the equilibration time on the frozen semen quality of duroc pig in modified tris-egg yolk with the addition of extracellular cryoprotectants. Semen is collected twice per week using massage methods from two duroc males aged ±2.5 years which in good health and have been trained in semen colection. The design used is a completely randomized design with 4 tests and 3 treatments T1: equilibration time of 1 hour, T2: equilibration time of 2 hours, T3: equilibration time of 3 hours at a temperature of 50C. Observed parameters were motility, viability, abnormality, MPU, and recovery rate (RR). The results showed that motility, viability, abnormalities, MPU, and RR were higher (P<0.05) at 2 hours of equilibration (25.63±3.15%, 55.86±5.41%, 4.92±0.53%, 56.69±5.64%, 36.61±4.49%) compared to 1 and 3 hours (20.00±3.54%, 39.28±9.89%, 7.93±0.76%, 40.21±9.39%, 28.57±5.05%) and (15.00±4.08%, 32.19±7.0%, 8.41±0.70%, 32.71±7.07%, 21.43±5.83%). It was concluded the motility of duroc pig spermatozoa in the tris-modified diluents after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the requirement of 40% spermatozoa motility for artificial insemination. It was concluded that the sperm motility of Duroc pig in tris-modified diluent after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the standard of 40% spermatozoa motility for artificial insemination.

Downloads

Download data is not yet available.

References

Azizah, Arifiantini R. 2009. Kualitas semen beku kuda pada pengencer susu skim dengan konsentrasi gliserol yang berbeda. Jurnal Veteriner 10(2): 63-70.
Bearden H, Fuquay J, Willard S. 2004. Applied Animal Reproduction (6th ed.). Pearson Prentice Hall.
Bebas W, Gorda W. 2020. Kadar krioprotektan gliserol dan dimethylsulfoxide terbaik pada pengencer astaxanthin fosfat kuning telur bebek terhadap kualitas semen beku babi. Jurnal Veteriner 21(1): 115-123.
Febriani G, Hamdan, Melia J. 2014. Pengaruh waktu ekuilibrasi terhadap kualitas semen kerbau lumpur (Bubalus bubalis) Setelah thawing. Jurnal Medika Veterinaria 8(1): 1-4.
Garner D, Hafez E. 200). Spermatozoa and Seminal Plasma. In B. Hafez & E. Hafez (Eds.), Reproduction in farm animals (7th ed.). Lippincott Williams & Wilkins.
Han X, Niu Z, Liu F, Yang C. 2005. Effects of diluents, cryoprotectants, equilibration time and thawing temperature on cryopreservation of duck semen. International Journal of Poultry Science 4(4): 197-201.
Hoesni F. 1997. Pengaruh kadar kuning telur dalam berbagai pengencer terhadap kualitas spermatozoa domba pasca pembekuan. Tesis. Program Pasca Sarjana Universitas Padjadjaran. Bandung.


Holt W. 2000. Basic aspects of frozen storage of semen. Animal Reproduction Science 62(1-3). 3-22.
Johnson, L., Weitze, K., Fiser, P., & Maxwell, W. (2000). Storage of boar semen. Animal Reproduction Science 62(1-3): 143-172.
Okazaki T, Abe S, Shimada M. 2009. Improved conception rates in sows inseminated with cryopreserved boar spermatozoa prepared with a more optimal combination of osmolality and glycerol in the freezing extender. Journal Animal Science 80(2): 121-129.
Patmawan EF, Gaina CD, Foeh ND. 2020. Morfologi abnormalitas spermatozoa babi landrace dan babi duroc dengan pewarnaan carbofuchsin. Jurnal Veteriner Nusantara 3(2): 113-119.
Rizal M, Herdis, Boediono A, Aku A, Yulnawati. 2006. Peranan beberapa jenis gula dalam meningkatkan kualitas semen beku domba garut. Jurnal Ilmu Ternak Dan Veteriner 11(2): 123-130.
Salamon S, Maxwell W. 2000. Storage of Ram Semen. Animal Reproduction Science 62(1-3): 77-111.
Sangma T, Ahmed K, Choudhury M, Zaman G, Ahmed N, Das A. 2020. Characteristics of fresh crossbred hampshire boar semen. Haryana Veterinarian 59(1): 98-101.
Solihati N, Rasad SD, Setiawan R, Nurjanah S. 2018. Pengaruh Kadar Gliserol Terhadap Kualitas Semen Domba Lokal. Jurnal Biodjati 3(1): 63.
Steel RGD, Torrie JH. 1995. Prinsip dan Prosedur Statistik: Suatu Pendekatan Biometrik. Sumantri B. (penerjemah). Gramedia Pustaka Utama, Jakarta
Suharman H. 2016. Kualitas semen beku domba garut (Ovis Aries) pada penambahan sukrosa dalam pengencer semen tris kuning telur. Jurnal Ilmu-Ilmu Hayati 16(1): 31-38.
Sumardani N, Tuty L, Siagian P. 2008. Viabilitas spermatozoa babi dalam pengencer bts (beltsville thawing solution) yang dimodifikasi pada penyimpanan berbeda. Jurnal Media Peternakan 31(2): 81-86.
Surachman M, Herdis, Yulnawati, Rizal M, Maheshwari H. 2008. Kualitas semen cair asal epididimis kerbau belang dalam bahan pengencer andromed yang mendapat penambahan sukrosa. Media Peternakan 32(2): 88-94.
Toelihere MR. 1993. Inseminasi Buatan pada Ternak. Penerbit Angkasa.
Viswanath R, Shanon P. 2000. Storage of bovine semen in liquid frozen state. Animal Reproduction Science 62(1-3): 23-53.
Waluwanja Y, Nalley WM, Hine TM, Ully K. 2019. Efektivitas berbagai konsentrasi minyak zaitun ekstra virgin oleum olivae) dalam pengencer sitrat kuning telur terhadap kualitas semen cair babi duroc. Jurnal Nukleus Peternakan 6(2): 55-62.
Yulianti ER. 2006. Pengaruh beberapa pengencer dengan waktu ekuilibrasi yang berbeda terhadap kualitas semen kambing boer sebelum pembekuan. Skripsi. Fakultas Peternakan UB Malang.
Yusuf T, Arifiantini R, Dapawole R, Nalley WM. 2017. Kualitas semen beku babi dalam pengencer komersial yang disuplementasi dengan trehalosa. Jurnal Veteriner 18(1): 69-75.
Published
2022-06-30
How to Cite
Koelima, B., Belli, H., Kihe, J., & Nalley, W. (2022). PENGARUH LAMA EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS-MODIFIKASI DENGAN PENAMBAHAN KRIOPROTEKTAN (Effect of equilibration time on frozen sement quality of the duroc pig in tris diluents modification With. JURNAL NUKLEUS PETERNAKAN, 9(1), 92-100. https://doi.org/10.35508/nukleus.v9i1.5490
Section
Articles

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.