• Henderiana L.L. Belli universitas nusa cendana
  • Filemon Y. Lanata Universitas Nusa Cendana
  • Petrus Kune Universitas Nusa Cendana
  • Imanuel Benu Universitas Nusa Cendana
Keywords: progesterone, hCG, artificial insemination


This study determined whether the administration of progesterone and hCG after insemination increased pregnancy rate in Bali Timor cows. A total of 30 Bali Timor cows (BW ± 200-225 kg; aged 3-7 years old) with different parity were used in this study. Cows were inseminated with progesterone and hCG. Cows were grouped based on body weight and number of parities following a Complete Randomized Design with 3 treatments and 10 replications. The treatments were: P1 = did not receive any treatment (control); P2 = estrus induced by PGF2α 125 mg on day 5, 7 and 9 (n = 10); and P3 = estrus induced by hCG 1000 IU on day 5 after artificial insemination (AI). Blood samples were collected from the jugular vein of the animals on day 0 and continued for a period of 33 days after AI using 5 mL heparinised pre-set syringes Blood samples were then centrifuged at 1500 rpm for 10 minutes. Serum was harvested and stored at -20oC until serum concentrations of progesterone and hCG were determined using ELISA procedures. Pregnancy was determined by rectal palpation. The results showed that the AI pregnancy rates was greater in group treated with hCG (70%) than cows induced with progesterone (50%) or the control group. The concentrations of both progesterone and hCG were increased simultaneously on day 5 to 11 post AI. In conclusion, progesterone and hCG application in Bali Timor cows after artificial insemination could increase pregnancy rates.


Download data is not yet available.


Akhtar MS, Saleem M, Ahmad E, Ahmad T, Lashari MH, Ayaz MM, Kodhi LA, Ahmad I, Hussain I, Akhtar M. 2018. Effect of lecirelin acetate, HCG or progesteron admisistration on day 7 post-insemination on conception rate and progesterone concentration in crossbreed cattle. Iraqi Journal of Veterinary Sciences 32(2): 149-153.
Fonseca JF, Silva Filho JM, Palhares MS. 2001. Concentracao plasm atica de progesterone em novilhas receptoras submetidas a administracao the rbST, GnRH ou HCG no quinto dia do ciclo estral. Arq Brasileri Medic Veteri Zootec 53:451-458.
Garrett JE, Geisert RD, Zavy MT and Morgan GL 1988. Evidence for maternal regulation of early conceptus growth and development in beef cattle. Journal of Reproduction and Fertility 84: 437-446.
Geisert RD, Morgan GL, Short EC, Zavy MT. 1992. Endocrine events associated with endometrial function and conceptus development in cattle. Reprod. Fertil. Dev. 4: 301-305.
Ireland JJ, Murphree RL, Coulson PB. 1980. Accuracy of predicting stages of bovine estrous cycle by gross appearance of the corpus luteum. J. Dairy Sci. 63: 155-160.
Mann GE, Lamming GE 2001. Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction 121: 175-180.
Mann GE, Fray MD and Lamming GE 2006. Effect of time of progesterone supplementation on embryo development and interferon-t production in the cow. Veterinary Journal 171: 500-503.
Mann GE, Lamming GE, Fisher PA. 1998b. Progesterone control of embryonic interferon-π production during early pregnancy in the cow. J. Reprod. Fertil. Abst Series 21:37 abstr.
Mann GE, Lamming GE, Robinson RS, Wathes DC. 1998a. The regulation of interferon-π production and uterin hormone receptors during early pregnancy. J. Reprod. Fertil. (Suppl), 54, (in press).
Meyer MD, Hansen PJ, Thatcher WW, Drost M, Badinga L, Roberts RM, Li J, Ott TL, Bazer FW. 1995. Extension of corpus luteum lifespan and reduction of uterine secretion of prostaglandin F2α cows in response to recombinant interferon-tau. J. Dairy Sci. 78: 1921-1931.
Monteiro PLJ, Ribeiro ES, Maciel MP, Dias ALG, Sole E, Lima FS, Bisinotto RS, Thatcher WW, Sartori R, Santos JEP. 2014. Effects of supplemental progesterone after artificial insemination on expression on interferon-stimulated genes and fertility in dairy cows. J. Dairy Sci. 97: 4907-4921.
Price CA, Webb R. 1989. Ovarian response to HCG treatment during the oestrous cycle in heifers. J Reprod Fertil 86: 303-308.
Robinson NA, Leslie KE, Walton JS. 1989. Effect of treatment with progesterone on pregnancy rate and plasma concentrations of progesterone in Holstein cows. J. Dairy Sci. 72: 202-207.
Santos JEP, Thatcher WW, Pool L, Overton MW. 2001. Effect of human chorionic gonadotropin on luteal function and reproductive performance of high-producing lactating Holstein dairy cows. J. Anim. Sci. 79: 2881-2894.
Santos JEP, Narciso CD, Rivera F, Thatcher WW, Chebel RC. 2010. Effect of reducing the period of follicle dominance in a timed artificial insemination protocol on reproduction of dairy cows. J. Dairy Sci. 93: 2976-2988.
Shams-Esfandabadi N, Shirazi A. 2007. Evaluation of effect of muscular injection of progesterone on days 2-5 following insemination on pregnancy rate in dairy cows. J. Biological Sciences 10(1): 152-155.
Souza AH, Gumen A, Silva EPB, Cunha AP, Guenther JN, Peto CM, Caraviello DZ, Wiltbank MC. 2007. Supplementation with estradiol-17β before the last gonadotropinreleasing hormone injection of the Ovsynch protocol in lactating dairy cows. J. Dairy Sci. 90: 4623-4634.
Spencer TE, Johnson GA, Bazer FW, Burghardt RC, Palmarini M. 2007. Pregnancy recognition and conceptus implantation in domestic ruminants: roles of progesterone, interferons and endogenous retroviruses. Reprod. Fertil. Dev. 19:65-78.
Sreenan JM, Diskin MG. 1986. The extent and timing of embryonic mortality in the cow. In: JM Sreenan and MG Diskin (eds), Embryo Mortality in Farm Animals. Martinus Nijhoff, Dordrecht, pp 1-11.
Starbuck GR, Darwash AO, Mann GE Lamming GE. 2001. The detection and treatment of post-insemination progesterone insufficiency in dairy cows. Fertility in the high yielding dairy cow, Birt. Society Animal Science Occasional Publication 2(26).
Stevenson JS, Portaluppi MA, Tenhouse DE, Lloyd A, Eborn DR, Kacuba S, DeJarnette JM. 2007. Intervention to artificial insemination: conception rates, pregnancy survival, and ovarian responses to GnRH, HCG, and progesterone. J Dairy Sci. 90: 331-340.
Stronge AJH, Sreenan JM, Diskin MG, Mee JF, Kenny DA, Morris DG. 2005. Post insemination milk peogesterone concentration and embryo survival in dairy cows. Theriogenology 64:1212-1224.
Vasconcelos JLM, Silcox RW, Rosa GJM, Pursley JR, Wiltbank MC. 1999. Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of estrous cycle in lactating dairy cows. Theriogenology 52: 1067-1078.
Villarroel A, Martino A, Bondurant RH, Deletang F, Sischo WM. 2004. Effect of post-insemination supplementation with PRID on pregnancy in repeat-breeder Holstein cows. Theriogenology 61: 1513-1520. Wathes DC, Taylor VJ, Cheng Z, Mann GE. 2003. Follicle growth, corpus luteum function and their effect on embryo development in postpartum dairy cows. Reproduction Suppl. 61: 219-237.
Walton JS, Halbert GW, Robinson NA, Leslie KE. 1990. Effect of progesteron and human chorionic gonadotropin administration five days posinsemination on plasma and milk concentrations of progesteron and pregnancy rates of normal and repeat breeder dairy cows. Can. J. Vet. Res. 54: 305-308.
How to Cite

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.