PENGARUH BREED TERHADAP MOTILITAS SPERMA SAPI LOKAL INDONESIA DAN Bos taurus DI BALAI INSEMINASI BUATAN (Effect breeds on sperm motility of indonesian local bulls and bos taurus at Artificial Insemination Center)
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M. Hasan(1)
Universitas Djuanda
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Annisa Rahmi(2*)
Universitas Djuanda
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R. Handarini(3)
Universitas Djuanda
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T. Maulana(4)
Badan Riset dan Inovasi (BRIN)
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S. Said(5)
Badan Riset dan Inovasi (BRIN)
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E. M. Kaiin(6)
Badan Riset dan Inovasi (BRIN)
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A. Baharun(7)
Universitas Djuanda
(*) Corresponding Author
Abstract
The attempt to achieved the goal could be through using artificial insemination (AI) by frozen semen. This study aims to evaluate the effect of the breed on sperm motility using Madura, Bali bulls and Bos taurus frozen semen. The experiment used local bull frozen semen (Madura and Bali bull breeds) and Bos taurus bull (Simmental and Limousin breeds) produced by Singosari’s artificial insemination center. Sperm motility evaluation was conducted using computer-assisted sperm analysis (CASA, Spermvision, Minitube®, Germany). The analyzing data uses a generalized linear model, one-way ANOVA using the SPSS 26 software. Duncan’s multiple range test (DMRT) was used to determine any significant differences. The result shows for total motility (TM) and progressive motility (PM) are 63.89±1.72% and 54.38±4.39% (Madura bull), 51.48±3.30% and 42.47±5.48% (Bali bull), 58.21±1.13% and 44.38±3.15% (Simmental bull), also 58.12±1.64% and 51.49±3.03% (Limousin bull). There is no differential sperm motility (P>0.005) in both parameters for all breeds. All frozen semen used in this study qualifies with Indonesian National Standards and is approved for use in the artificial insemination program.
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References
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Rosyada ZNA, Tumbelaka LI, Ulum MF, Solihin DD, Kaiin EM, Gunawan M, Harsi T, Suharto K, Purwantara B. 2021. Meta data analysis of cenception rate in relation to sperm motility in Madura superior bulls. The 1st Internasinal Conference on Livestock in Tropical Environment (ICLiTE-1). IOP Conf. Series: Earth and Environmental Science 902(2021):012048. doi: 10.1088/1755-1315/902/1/012048
Tekin K, Inanc ME, Daskin A. 2022. Relationship between CASA motility and kinetic parameters on bull fertility. Cryobiology 71(3):568. doi: 10.1016/j.cryobiol.2022.10.132
Zamuna AAKKM, Susilawati T, Ciptadi G, Marjuki. 2015. Perbedaan kualitas semen dan produksi semen beku pada berbagai bagsa sapi potong. Jurnal Ternak Tropika 16(2):1-6. doi: 10.21776/ub.jtapro. 2015.016.02.1.
Baharun A, Arifiantini RI, Yusuf TL. 2017. Freezing capability of Pasundan bull sperm using tris-egg yolk, tris-soy, and andromed® diluents. Jurnal Kedokteran Hewan 11(1):45-49. doi: 10.21157/j.ked.hewan.v11i1.5810
Baharun A, Said S, Arifiantini RI, Karja NWK. 2021. Correlation between age, testosterone and adiponectin concentration, and sperm abnormalities in Simmental bulls. Veterinary World 14(8):2124-2130. doi: 10.14202/vetworld.2021.2124-2130
Baharun A, Said S, Arifiantini RI, Karja NWK. 2022. Karakteristik Semen dan Korelasi antara Konsentrasi Testosteron dengan Libido Pejantan Sapi Simental. Acta Veterinaria Indonesiana 10(1):1-7. doi: https://doi.org/10.29244/avi.10.1.1-7
BSN (Badan Standarisasi Nasional). 2017. Semen Beku- Bagian 1: Sapi. Jakarta, Badan Standarisasi Nasional.
Herbowo MT, Arifiantini RI, Karja NWK, Sianturi RG. 2019. Cryopreservation of Swamp Buffalo semen in skim milk yolk-based diluent with two different. Tropical Animal Science Journal 42(1):1-13. doi: https://doi.org/10.5398/tasj.2019.42.1.13
Indriastuti R, Ulum MF, Arifiantini RI, Purwantara B. 2020. Individual variation in fresh and frozen semen of Bali bulls (Bos Sondaicus). Veterinary World 13(5):840-846. doi: 10.14202/vetworld.2020.840-846
Islam MM, Umehara T, Tsujita N, Shimada. 2021. Saturated fatty acids accelerate liniear motility through mitochondrial ATP production in bull sperm. Reproductive Medicine and Biology 20(3):289-298. doi: 10.1002/rmb2.12381
Kaya A, Memili E. 2016. Sperm macromolecules associated with bull fertility. Animal Reproduction Science 169:88-94. doi: 10.1016/j.anireprosci.2016.02.015
Khalil WA, El-harairy MA, Zeidan AB, Hassan MAE. 2018. Evaluation of bull spermatozoa during and after cryopreservation: Structural and ultrastructural insights. International journal of veterinary science and medicine 6(Supp 1): S49-S56. doi: 10.1016/j.ijvsm.2017.11.001
Mughal DH, Ijaz A, Yousaf MS, Wadood F, Farooq, Mahmood SA, Riaz A. 2017. Effect of osmotic pressure on spermatozoa characteristics of cryopreserved buffalo bul (Bubalus bubalis) semen. Journal of Applied Animal Research 46(1):274-277. doi: https://doi.org/10.1080/09712119.2017.1295971
Novianti I, Purwantara B, Herwijanti E, Nugraha CD, Putri RF, Furqon A, Septian WA, Rahayu S, Nurgiartiningsih VMA, Suyadi S. 2020. Effect of breed on semen characteristics of aged bulls in the Indonesian National Artificial Insemination Center. Jurnal Ilmu-Ilmu Peternakan 30(2):173-179. doi: 10.21776/ub.jiip.2020.030.02.10
O’Meara C, Henrotte E, Kupisiewicz K, Latour C, Broekhuijse M, Camus A, Gavin-Plagne L, Sellem E. 2022. The effect of adjusting settings within a Computer-Assisted Sperm Analysis (CASA) system on bovine sperm motility and morphology results. Animal Reproduction 19(1):1-13. doi: https://doi.org/10.1590/1984-3143-AR2021-0077
Peraturan Menteri Pertanian Republik Indonesia (Permentan). 2016. Penyediaan dan peredaran semen beku ternak ruminansia nomor: 10/Permentan/PK.210/3/2016. Jakarta: Kementan.
Raafi M, Yusuf M, Toleng AL, Diansyah AM, Surahman, Sahiruddin. 2021. Movement patterns of sperms at different bull breeds using computer-assisted sperm analysis (CASA). The 3rd International Conference of Animal Science and Technology. IOP Conf. Series: Earth and Environmental Science. 788(2021)012137. doi:10.1088/1755-1315/788/1/012137
Rodriguez-Villamil P, Hoyos-Marulanda V, Martins JA, Oliveira AN, Aguiar LH, Moreno FB, Velho AL, Monteiro-Moreira AC, Moreira RA, Vasconcelos IM, Bertolini M, Moura AA. 2016. Purification of binder of sperm protein 1 (BSP1) and its effects on bovine in vitro embryo development after fertilization with ejaculated and epididymal sperm. Theriogenology 85(3):540-54. doi: https://doi.org/10.1016/j.theriogenology.2015.09.044
Rosyada ZNA, Tumbelaka LI, Ulum MF, Solihin DD, Kaiin EM, Gunawan M, Harsi T, Suharto K, Purwantara B. 2021. Meta data analysis of cenception rate in relation to sperm motility in Madura superior bulls. The 1st Internasinal Conference on Livestock in Tropical Environment (ICLiTE-1). IOP Conf. Series: Earth and Environmental Science 902(2021):012048. doi: 10.1088/1755-1315/902/1/012048
Tekin K, Inanc ME, Daskin A. 2022. Relationship between CASA motility and kinetic parameters on bull fertility. Cryobiology 71(3):568. doi: 10.1016/j.cryobiol.2022.10.132
Zamuna AAKKM, Susilawati T, Ciptadi G, Marjuki. 2015. Perbedaan kualitas semen dan produksi semen beku pada berbagai bagsa sapi potong. Jurnal Ternak Tropika 16(2):1-6. doi: 10.21776/ub.jtapro. 2015.016.02.1.
Published
2023-06-18
How to Cite
Hasan, M., Rahmi, A., Handarini, R., Maulana, T., Said, S., Kaiin, E., & Baharun, A. (2023). PENGARUH BREED TERHADAP MOTILITAS SPERMA SAPI LOKAL INDONESIA DAN Bos taurus DI BALAI INSEMINASI BUATAN (Effect breeds on sperm motility of indonesian local bulls and bos taurus at Artificial Insemination Center). JURNAL NUKLEUS PETERNAKAN, 10(1), 21-26. https://doi.org/10.35508/nukleus.v10i1.8930
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