IDENTIFIKASI SPERMA PEMBAWA KROMOSOM X DAN Y SECARA MORFOMETRI SEBAGAI DASAR APLIKASI SEXING SEMEN SAPI PASUNDAN (Morphometrics identification of x and y chromosome bearing sperm as a basis for semen sexing application in Pasundan cattle)

  • Nurcholidah Solihati(1*)
    Universitas Padjadjaran
  • Siti Darodjah Rasad(2)
    Universitas Padjadjaran
  • Nena Hilmia Nena Hilmia(3)
    Universitas Padjadjaran
  • Salsa Adila Irianti(4)
    Universitas Padjadjaran
    • (*) Corresponding Author
DOI: https://doi.org/10.35508/nukleus.v10i2.13323

Abstract

Pasundan cattle spread across the West Java region, but their population decreased from 2014 to 2019. Accelerated population increase has been carried out through the artificial insemination (AI) and it is hoped can help meet the need for meat. AI program can be accompanied by the application of sperm sexing so it can meet the sex according to purpose. The method of albumin column is sexing method based on morphometric data. The aim of this research was to identify the X and Y crhomosome bearing sperm morphometrically as a basis for sperm sexing in Pasundan cattle semen. This research used frozen semen from seven Pasundan cattle. Sperm morphometric observations were carried out using an Olympus microscope equipped with the DP2-BSW-E application. The parameters observed included the length, width and area of the sperm head and the proportion of X and Y chromosome bearing sperm. Two straws were used from each bull, each straw was counted as 200 sperm, so the number of sperm measured was 400 sperm per bull. The data was analyzed quantitatively descriptively. The results of measurements on seven males showed that the sperm head of Pasundan cattle had a minimum-maximum length of 7.68 - 11.36 µm; width 3.97 - 6.30 µm; sperm head area 31.09 - 51.12 µm2. The percentage 41.00-55.50% for X- and 44.50-59.00% for Y- chromosome bearing sperm, with average 50.25% for X- and 49,75% for Y-chromosome bearing sperm. It was concluded that the X and Y chromosome bearing sperm have balanced natural proportion, so the sperm sexing needs to be done to increase the chances of birth of the sex according to purpose.

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References

Arifin J, Daud AR, Anang A, dan Sudirja R. 2020. Dispersi Populasi Sapi Pasundan Berdasarkan Kondisi Geografis di Kabupaten Garut. Jurnal Produksi Ternak Terapan. 1 (1): 16-21. https://doi.org/10.24198/jptt.v1i1.27645
Arifin J , Sulasmi. 2019. Jarak Genetik Sapi Pasundan Melalui Pendekatan Kraniometri Antar Wilayah Pangandaran, Tasikmalaya dan Garut Jawa Barat. Jurnal Ternak. 10(1): 12-18. https://doi.org/10.30736/jy.v10i1.36
Bintara S. 2011. Rasio Spermatozoa X:Y dan Kualitas Sperma pada Kambing Kacang dan Peranakan Ettawa. Sains Peternakan 9(2): 65-7.
Chaudhary I, Jain M, Halder A. 2014. Sperm Sex Ratio (X: Y Ratio) and its Variations. Austin J Reprod Med Fertil, 1(1):7.
Direktorat Jenderal Peternakan dan Kesehatan Hewan. 2021. Sapi Pasundan, Plasma Nutfah Kebanggan Jawa Barat.
Elsadina FA, Arifin J, Rahmat D. 2021. Prospek Pengembangan Sumberdaya Genetik Sapi Pasundan Berdasarkan Nilai Efeketif dan Daya Dukung Wilayah di Kabupaten Garut. Jurnal Sumber Daya Hewan, 2(1): 22-29.
Garner DL, Gledhill BL, Pinkel D, Lake S, Sthepenson D, Van Dilla MA, Johnson LA. 1983. Quantification of the X- and Y-Chromosome-bearing spermatozoa of domestic animals by Flow Cytometer, Biology of Reproduction, 28, 312-321.
Hilmia N. 2013. Productivity and Genetic Diversity of Local Cattle in Ciamis-West Java. J of the Indonesian Tropical Animal Agriculture, vol. 38(1): 10-19, https://doi.org/10.14710/jitaa.38.1.10-19
Hilmia N, Rahmat D, Dudi , Hadi DN. 2019. Nucleotide Polymorphism on Exon 2 Leptin Gene of Pasundan Cattle. IOP Conf. Series: Earth and Environmental Science 334 (2019) 012013
Indrijani H, Arifin J, Dudi, Putranto W, Zamhur R, Hilmia N. 2012. Kajian Identifikasi Sapi Lokal Jawa Barat Dalam Mendukung Swasembada Daging Sapi. Laporan Penelitian. Dinas Peternakan provinsi Jawa Barat. Bandung.
Khairunissa S, Novelina S, Hadi DN, Ulum MF. 2021. Ultrasound Imaging to Estimate Carcass Quality of Pasundan Cattle based on Body Condition Score. Jurnal Ilmu Ternak dan Veteriner, 26(1)
Kusuma SB, Ngadiyono N, Sumadi A. 2017. Estimasi Dinamika Populasi dan Penampilan Reproduksi Sapi Peranakan Ongole Di Kabupaten Kebumen Provinsi Jawa Tengah. Buletin Peternakan. 41 (3): 230-242.
Lapoliwa AD, Isnaini N. 2021. Semen Production Characteristics of Pasundan Bull at Different Body Weight. Jurnal Agripet, 21(1): 1-4. DOI: https://doi.org/10.17969/agripet.v21i1.15186
Rahmat. 2020. Pengaruh Lama Sentrifugasi Pada Sexing Spermatozoa dengan Metode Sedimentasi Putih Telur Terhadap Kualitas Semen dan Rasio Spermatozoa X dan Y Pada Sapi Bali (Bos Sondaicus). Skripsi. Fakultas Peternakan Universitas Hasanuddin. Makassar.
Rasad SD, Solihati N, Winangun K, Yusrina A, Avicenna F. 2020. Effect of Incubation Time During Sperm Sexing Process on Sperm Quality of Pasundan Bull. Jurnal Ilmu Ternak dan Veteriner, 25(3): 112-119. DOI: http://dx.doi.org/10.14334/jitv/v25i3.2494
Santoso H, Arifiantini RI, Gunawan A, Sumantri C. 2021. Characteristics and Potential Production of Frozen Semen of Pasundan Bull. Tropical Animal Science Journal, 44(1):24-31. DOI: https://doi.org/10.5398/tasj.2021.44.1.24

Setiawati EN, Saleh DM, Sumaryad MY. 2018. Kinerja Reproduksi Sapi Pasundan di Jawa Barat. Prosiding Seminar Nasional Teknologi dan Agribisnis Seri VI. Fak Peternakan Unsoed:.158-167. https://jnp.fapet.unsoed.ac.id/index.php/psv/article/view/150
Setiawati EN, Sumaryadi MY, Saleh DM, Armelia V. 2021. Effect of Estrus Synchronization with Prostaglandins (PGF2Α) and Gonadothropin Realising Hormone (GNRH) on the Hematological Profile of Pasundan Heifers during Pregnancy. Inter J of Environment, Agriculture and Biotechnology 6(2). DOI: 10.22161/ijeab
Solihati N, Rasad SD, Yusrina A, Dimyati YI. 2017. Identifikasi Morfometrik Sperma Domba Lokal sebagai Dasar Aplikasi Sexing Sperma. Jurnal Ilmu Ternak, 17(2): 109-113.DOI: 10.24198/jit.v17i1.15138
Solihati N, Rasad SD, Yusrina A. 2017. Evaluation the natural proportion of X-Y chromosome bearing sperm of West Java Local Ram using morfometric methode. Proceeding 7th International Seminar on Animal Production (ISTAP). Universitas Gadjah Mada, Yogyakarta.
Solihati N, Rasad SD, Hilmia N, Winangun K, Toha T. 2023. X-Y Chromosome bearing Sperm Proportion of Local Ram after Sexing with Different Combination of Bovine Serum Albumin (BSA) Concentration. Animal Production, 25(1): 8-13.
Susandani O, Suprayogi TW, Damayanti R, Ma’ruf A. 2021. Factors Affecting Fresh Semen Quality in Pasundan Cattle at UPTD BPPIBTSP Ciamis. J of Applied Veterinary Science and Tecnology. 37-42. DOI: 10.20473/javest.V2.I2.2021.37-42
Susilawati T, Sumitro SB, Harjopranjoto S, Mantara Y, Nuryadi. 1999. Pola kapasitasi spermatozoa X dan Y sapi hasil pemisahan menggunakan filtrasi sephadex dan sentrifugasi gradien densitas percoll. Jurnal Penelitian Ilmu-Ilmu Hayati 11: 29-40.
Susilawati T. 2014. Sexing Spermatozoa. Malang: UB Press
Tawaf R. 2017. Karakteristik Usaha Ternak Sapi Pasundan di Jawa Barat. Jurnal Nukleus Peternakan 4(1): 95-101.

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Published
2023-12-30
How to Cite
Solihati, N., Rasad, S., Nena Hilmia, N. H., & Irianti, S. (2023). IDENTIFIKASI SPERMA PEMBAWA KROMOSOM X DAN Y SECARA MORFOMETRI SEBAGAI DASAR APLIKASI SEXING SEMEN SAPI PASUNDAN (Morphometrics identification of x and y chromosome bearing sperm as a basis for semen sexing application in Pasundan cattle). JURNAL NUKLEUS PETERNAKAN, 10(2), 44-51. https://doi.org/10.35508/nukleus.v10i2.13323
Issue
Vol 10 No 2 (2023): Desember
Section
Articles

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