Analisis Kandungan Metabolit Sekunder dan Uji aktivitas Antibakteri Jamur Endofit Batang Binahong (Anredera COrdifiolia Steenis)
-
Antonius R B Ola(1*)
Chemistry Department, NUsa Cendana University, KUpang, NTT
-
Elisabeth Indriyani Paula Bria, Ms(2)
Chemistry Department, NUsa Cendana University, KUpang, NTT
-
Theo da Cunha, Mr(3)
Chemistry Department, NUsa Cendana University, KUpang, NTT
(*) Corresponding Author
Keywords:
Endophytic fungi, antibacterial, binahong
Abstract
The isolation of endophytic fungi (penicillium sp) from binahong stem had been undertaken with the objective to determine its content of secondary metabolites and its antibacterial activity. The content of secondary metabolites was analyzed with HPLC and its antibacterial activity was measured using paper disc diffusion method. The results showed that there were four compounds in the HPLC chromatogram detected at 235 nm. The antibacterial activity for methanol and ethyl acetate extracts were observed for its diameter of zone inhibition at 18.2 mm and 16.7 mm for S.aureus and 17.8 mm and 16.3 mm for E. Coli.
Downloads
Download data is not yet available.
References
Aly, Amal H, Abdessamad Debbab, Julia Kjer, dan Peter Proksch.2010. Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products. Fungi Diversity 41(1), 1-16.
Bhardwaj S. K., Dwivedia K. and Agarwala D. D. (2015). A Review: HPLC Method Development and Validation. International Journal of Analytical and Bioanalytical Chemistry, 5(4): 76-81.
Brian J. Millar, Sanjukta Deb. (2014). Effect of Autoclave Sterilisation on the Dimensional Stability and Tear Strength of Three Silicone Impression Materials. Scientific Research Publishing Inc. 4: 518-526.
Darsana, I.G.O., Besung, I.N.K., & Mahatmi, H. (2012). Potensi daun BInahong (A`2nredera Crdifolia (tenore) Stenis) dalam menghambat pertumbuhan bakteri Escherichia Coli secara In Vitro. Indonesia Medicus Veterinus, 1(3), 337-351.
Djarijah, M dan Djarijah, A.S. 2001. Budidaya jamur tiram putih. Universitas Negeri Malang : Malang
Ekaviantiwi, TA, E Fachriyah, and D Kusrini. 2013. Iedntifikasi asam fenolat dari ekstrak etanol daun binahong (Anredera Cordifolia (Ten) Stennis) dan uji aktivitas antioksidan. Chem. Info. 1(1): 283-293.
Gandjar, I., Robert, A.S., Karin, T,V., Oetari, A., Santoso, I., 1999, Pengenalan Kapang Tropik Umum, hal 7, ISBN 979-461-289-8, Jakarta : Yayasan Obor Indonesia.
Gutierrez, R.M.P., Gonzalez, A.M.N., and Ramirez, A.M., 2012, Compounds Derived from Endophytes: A Review of Phytochemistry and Pharmacology., 19, 2992-3030.
Harborne, J.B. (1987). Metode fitokimia: penuntun cara modern menganalisis tumbuhan. Diterjemahkan oleh Padmawinta, K., & Soediro, I. Bandung, Indonesia: ITB Press.
Hammerschmidt, L., Ola, A., Werner, E. G. M., WenHan, L., Attila, M., Tibor, K., Peter, P., Amal, H. A., 2015, Two New Metabolites from the Endophytic Fungus Xylaria sp. Isolated from the Medicinal Plants Curcuma Xanthorrhiza, Tetrahedron Letters., 56, 1193–1197.
Huang et al., 2008, Chemistry and weak antimicrobial activities of phomopsins produced by mangrove endophytic fungus of phomopsins sp., ZHU_H76. Phytochemistry., 69, 1604-1608.
Kjer, J., Wray, V., Edrada-Ebel, R. A., Ebel, R., Pretsch, A., Lin, W. H., 2009, Proksch P. Xanalteric Acids I and II and Related Phenolic Compounds from an Endophytic Alternaria sp. Isolated from the Mangrove Plant Sonneratia Alba, J Nat Prod., 72, 2053-2057
Kour, A., Shawl, A. S., Rehman, S., Sultan, P., Qazi, P. H., Suden, P., Khajuria, R. K., Verma, V., 2008, Isolation and Identification of an Endophytic Strain of Fusarium Oxysporum Producing Podophyllotoxin from Juniperus Recurva, World Journal of Microbiology and Biotechnology., 24, 1115-1121.
Kusari, Souvik, Christian Hertweck, dan Michael Spiteller.2012. Chemical Ecology of Endophytic Fungi: Origins of Secondary Metabolites. Chemistry & Biology 19(7), 792-98.
Liu Y., Lee M.L. 2006. Ultrahigh pressure liquid chromatography using elevated temperature. Journal of Chromatography. 1104 (1-2): 198–202.
Losgen, S., Magull, J., Schulz, B., Draeger, S., and Zeeck, A., 2008, Isofusidienols: Novel Chromone-3-Oxepines Produced by the Endophytic Fungus Chalara sp., Eurp J, Org Chem., 4, 698-703.
Manoi F. Warta Penelitian dan Pengembangan Tanaman Industri. 2009;15(1):h 3-5.
Martin M., Guiochon, G. 2005. Effects of high pressures in liquid chromatography. J. Chromatogr. A (1-2)7: 16-38.
Nisa, H., Kamili, N. Asra., Nawcho, A. Irshad., Shafi, Sana., 2015, Fungal Endophytes Prolific Source of Phytochemical and Other Bioctive Natural Product : A Review. Mikroba Patogenesis xxx (2015) 1-10.
Neolaka, Yustus. 2011. Penuntun Praktikum Kimia analitik. Lab. Pendidikan Kimia. FKIP UNDANA : Kupang.
Ola, A. R. B., Amal, H. A., Ilka, Z., Alexandra, H., Attila, M., Matthias, K., Heike, B. O., Tibor, K., Peter, Proksch., Abdessamad, D., 2014, Absolute Configuration and Antibiotic Activity of Neosartorin from the Endophytic Fungus Aspergillus fumigatiaffinis, Tetrahedron Letters., 55, 1020–1023.
Ola, A. R. B., Amal, H. A., Ilka, Z., Alexandra, H., Attila, M., Matthias, K., Heike, B. O., Tibor, K., Peter, Proksch., Abdessamad, D., 2014, Absolute Configuration and Antibiotic Activity of Neosartorin from the Endophytic Fungus Aspergillus fumigatiaffinis, Tetrahedron Letters., 55, 1020–1023.
Ola, A. R. B., Amal, H, A., Wen, H. L., Victor, W., Abdessamad, D., 2014, Absolute configuration and Revision of the Structure of Lateritin, Tetrahedron Letters., 55, 3147–3150.
Ola, A. R. B., Heike, B. O., Abdessamad, D., Peter, P., and Amal, H. A., 2014, Dihydroanthracenone Metabolites from the Endophytic Fungus Diaporthe melonis Isolated from Annona squamosa, Tetrahedron Letters., 55, 3133–3136.
Petrini, O, Sieber TN, Toti L, dan Viret, O. 1992. Ecology metabolite production and substrate utilization in endophytic fungi. Natural roxins. Vol. 1 : 185
Pratama, M. R. 2005. Pengaruh ekstrak jeruk kayu siwak (Salvadora persica) terhadap pertumbuhan bakteri staphylococus mutans dan S. Aureus dengan metode difusi agar. Laporan hasil penelitian program studi biologi Fakultas matematika dan ilmu pengetahuan alam Institut teknologi sepuluh November.
Prescott, S.C, dan Dun. 1959. Industrial Microbiology 3 Ed. International Student Edision. McGrawHill Book Company : New York.
Puri, S. C., Nazir, A., Chawla, R., Arora, R., Riyaz, U. H. S., Amna, T., Ahmed, B., Verma, V., Singh, S., Sagar, R., Sharma, A., Kumar, R., Sharma, R. K., Qazi, G. N., 2006, The endophytic fungus trametes hirsute as a novel alternative source of podophyllotoxin and related aryl tetralin lignans, J Chem Ecol., 34, 32-38.
Prakash, Ved.2015. Endophytic Fungi As Resource of Bioactive Compounds, 15.
Rachmawati, S. 2007. Studi Makroskopi, dan Skrining Fitokimia Daun BInahong (Anredera Cordifolia (Ten) Steenis). Fakultas Farmasi UNAIR Surabaya.
Rendowaty, A., Djamaan, A., and Handayani, D., 2017, Waktu Kultivasi Optimal dan Aktivitas Antibakteri dari Ekstrak Etil Asetat Jamur Simbion Aspergillus unguis (WR8) dengan Haliclona fascigera., 4(2), 49–54.
Rimporok, S., Kepel, B. J., Siagian, K. V., 2015, Uji Aktivitas Ekstrak Daun Binahong (Anredera Cordifiolia Steenis) Terhadap Pertumbuhan Streptococcus Mutans Secara In Vitro, Jurnal Ilmiah Farmasi – UNSRAT Vol. 4 No. 4 2302 – 2493
Robinson, T. (1995). Kandungan Organik Tumbuhan Tingkat Tinggi. Diterjemahkan oleh Padmawinata, K. Bandung, Indonesia : ITB Press.
Sulistyani S. Uji Efek Ekstrak Etanol 70% Daun Binahong (Anredera Cordifolia Steenis) Terhadap Kadar Alt ( Alanin Aminotransferase) pada Tikus Jantan Galur Wistar ( Rattus Norvegicus) yang Di Induksi dengan Parasetamol. Kedok Journal. 2013. h.3.
Saifudin, A., 2014, Senyawa alam metabolit sekunder teori, konsep dan teknik pemurnian Ed.1, Yogyakarta: Budi Utama. ISBN 978-602-280-472-7, 113 hal.
Strobel, G. A., Daisy, B. H., Castilo, U., Harper, J., 2004, Natural products from endophytic microorganisms, J Nat Prod., 67, 257-268.
Strobel, G., and Daisy, B., 2003, Bioprospecting for Microbial Endophytes and Their Natural Products, Microbiology and Molecular Biology Reviews., 67, 491-502.
Song, Hong-Chuan, Dan Qin, Mei-Jun Han, Ling Wang, Kou Zwang, dan Jin-Yan Dong.2017. Bioactive 2-Pyrone Metabolites From An Endophyc Phomopsis Asparagi SWUKJ%.2020 of Kadsura Angustifolia. Phytochemistry Letters 22, 235-40.
Shou-Jie LI, Xuang Zhang, Xiang-Hua Wang, dan Chang-Qi Zhao.2018. Novel natural compounds from endophytic fungi with anticancer actifity. European Journal of Medicinal Chemistry 156, 316-43.
Verma, V. C., Kharwan, R. N., Strobel, G. A., 2009, Chemical and fungsional diversity of natural products from plant associated endophytic fungi, Nat Prod Commun., 41, 511-654
Visagie CM, Houbraken J, Frisvad JC, et al. 2014. Identification and nomen- clature of the genus Penicillium. Studies in Mycology 78: 343–371.
Xu et al., 2009, Chromones from the endophytic fungus pestalotiopsis sp. Isolated from the Chinese mangrove plant Rhyzopora mucronata, J Nat Prod., 72, 662-665.
Xu, Lijian, Wei Meng, Cong Cao, Jiang Wang, Wenjun Shan, dan Qinggui Wang. 2015. Antibacterial and Antifungal Compounds from Marine Fungi. Marine Drugs 13(6), 3479-3513.
Zhao, J., T, Shan., Y, Mou., and L, Zhou., 2011, Plant-Derived Bioactive Compounds Produced by Endophytic Fungi, Mini Reviews in Medicinal Chemistry., 11, 159-168.
Zhang, H. W., Song, Y. C., and Tan, R.X., 2016, Biology and chemistry of endophytes,. 2001, 18, 448
.
Bhardwaj S. K., Dwivedia K. and Agarwala D. D. (2015). A Review: HPLC Method Development and Validation. International Journal of Analytical and Bioanalytical Chemistry, 5(4): 76-81.
Brian J. Millar, Sanjukta Deb. (2014). Effect of Autoclave Sterilisation on the Dimensional Stability and Tear Strength of Three Silicone Impression Materials. Scientific Research Publishing Inc. 4: 518-526.
Darsana, I.G.O., Besung, I.N.K., & Mahatmi, H. (2012). Potensi daun BInahong (A`2nredera Crdifolia (tenore) Stenis) dalam menghambat pertumbuhan bakteri Escherichia Coli secara In Vitro. Indonesia Medicus Veterinus, 1(3), 337-351.
Djarijah, M dan Djarijah, A.S. 2001. Budidaya jamur tiram putih. Universitas Negeri Malang : Malang
Ekaviantiwi, TA, E Fachriyah, and D Kusrini. 2013. Iedntifikasi asam fenolat dari ekstrak etanol daun binahong (Anredera Cordifolia (Ten) Stennis) dan uji aktivitas antioksidan. Chem. Info. 1(1): 283-293.
Gandjar, I., Robert, A.S., Karin, T,V., Oetari, A., Santoso, I., 1999, Pengenalan Kapang Tropik Umum, hal 7, ISBN 979-461-289-8, Jakarta : Yayasan Obor Indonesia.
Gutierrez, R.M.P., Gonzalez, A.M.N., and Ramirez, A.M., 2012, Compounds Derived from Endophytes: A Review of Phytochemistry and Pharmacology., 19, 2992-3030.
Harborne, J.B. (1987). Metode fitokimia: penuntun cara modern menganalisis tumbuhan. Diterjemahkan oleh Padmawinta, K., & Soediro, I. Bandung, Indonesia: ITB Press.
Hammerschmidt, L., Ola, A., Werner, E. G. M., WenHan, L., Attila, M., Tibor, K., Peter, P., Amal, H. A., 2015, Two New Metabolites from the Endophytic Fungus Xylaria sp. Isolated from the Medicinal Plants Curcuma Xanthorrhiza, Tetrahedron Letters., 56, 1193–1197.
Huang et al., 2008, Chemistry and weak antimicrobial activities of phomopsins produced by mangrove endophytic fungus of phomopsins sp., ZHU_H76. Phytochemistry., 69, 1604-1608.
Kjer, J., Wray, V., Edrada-Ebel, R. A., Ebel, R., Pretsch, A., Lin, W. H., 2009, Proksch P. Xanalteric Acids I and II and Related Phenolic Compounds from an Endophytic Alternaria sp. Isolated from the Mangrove Plant Sonneratia Alba, J Nat Prod., 72, 2053-2057
Kour, A., Shawl, A. S., Rehman, S., Sultan, P., Qazi, P. H., Suden, P., Khajuria, R. K., Verma, V., 2008, Isolation and Identification of an Endophytic Strain of Fusarium Oxysporum Producing Podophyllotoxin from Juniperus Recurva, World Journal of Microbiology and Biotechnology., 24, 1115-1121.
Kusari, Souvik, Christian Hertweck, dan Michael Spiteller.2012. Chemical Ecology of Endophytic Fungi: Origins of Secondary Metabolites. Chemistry & Biology 19(7), 792-98.
Liu Y., Lee M.L. 2006. Ultrahigh pressure liquid chromatography using elevated temperature. Journal of Chromatography. 1104 (1-2): 198–202.
Losgen, S., Magull, J., Schulz, B., Draeger, S., and Zeeck, A., 2008, Isofusidienols: Novel Chromone-3-Oxepines Produced by the Endophytic Fungus Chalara sp., Eurp J, Org Chem., 4, 698-703.
Manoi F. Warta Penelitian dan Pengembangan Tanaman Industri. 2009;15(1):h 3-5.
Martin M., Guiochon, G. 2005. Effects of high pressures in liquid chromatography. J. Chromatogr. A (1-2)7: 16-38.
Nisa, H., Kamili, N. Asra., Nawcho, A. Irshad., Shafi, Sana., 2015, Fungal Endophytes Prolific Source of Phytochemical and Other Bioctive Natural Product : A Review. Mikroba Patogenesis xxx (2015) 1-10.
Neolaka, Yustus. 2011. Penuntun Praktikum Kimia analitik. Lab. Pendidikan Kimia. FKIP UNDANA : Kupang.
Ola, A. R. B., Amal, H. A., Ilka, Z., Alexandra, H., Attila, M., Matthias, K., Heike, B. O., Tibor, K., Peter, Proksch., Abdessamad, D., 2014, Absolute Configuration and Antibiotic Activity of Neosartorin from the Endophytic Fungus Aspergillus fumigatiaffinis, Tetrahedron Letters., 55, 1020–1023.
Ola, A. R. B., Amal, H. A., Ilka, Z., Alexandra, H., Attila, M., Matthias, K., Heike, B. O., Tibor, K., Peter, Proksch., Abdessamad, D., 2014, Absolute Configuration and Antibiotic Activity of Neosartorin from the Endophytic Fungus Aspergillus fumigatiaffinis, Tetrahedron Letters., 55, 1020–1023.
Ola, A. R. B., Amal, H, A., Wen, H. L., Victor, W., Abdessamad, D., 2014, Absolute configuration and Revision of the Structure of Lateritin, Tetrahedron Letters., 55, 3147–3150.
Ola, A. R. B., Heike, B. O., Abdessamad, D., Peter, P., and Amal, H. A., 2014, Dihydroanthracenone Metabolites from the Endophytic Fungus Diaporthe melonis Isolated from Annona squamosa, Tetrahedron Letters., 55, 3133–3136.
Petrini, O, Sieber TN, Toti L, dan Viret, O. 1992. Ecology metabolite production and substrate utilization in endophytic fungi. Natural roxins. Vol. 1 : 185
Pratama, M. R. 2005. Pengaruh ekstrak jeruk kayu siwak (Salvadora persica) terhadap pertumbuhan bakteri staphylococus mutans dan S. Aureus dengan metode difusi agar. Laporan hasil penelitian program studi biologi Fakultas matematika dan ilmu pengetahuan alam Institut teknologi sepuluh November.
Prescott, S.C, dan Dun. 1959. Industrial Microbiology 3 Ed. International Student Edision. McGrawHill Book Company : New York.
Puri, S. C., Nazir, A., Chawla, R., Arora, R., Riyaz, U. H. S., Amna, T., Ahmed, B., Verma, V., Singh, S., Sagar, R., Sharma, A., Kumar, R., Sharma, R. K., Qazi, G. N., 2006, The endophytic fungus trametes hirsute as a novel alternative source of podophyllotoxin and related aryl tetralin lignans, J Chem Ecol., 34, 32-38.
Prakash, Ved.2015. Endophytic Fungi As Resource of Bioactive Compounds, 15.
Rachmawati, S. 2007. Studi Makroskopi, dan Skrining Fitokimia Daun BInahong (Anredera Cordifolia (Ten) Steenis). Fakultas Farmasi UNAIR Surabaya.
Rendowaty, A., Djamaan, A., and Handayani, D., 2017, Waktu Kultivasi Optimal dan Aktivitas Antibakteri dari Ekstrak Etil Asetat Jamur Simbion Aspergillus unguis (WR8) dengan Haliclona fascigera., 4(2), 49–54.
Rimporok, S., Kepel, B. J., Siagian, K. V., 2015, Uji Aktivitas Ekstrak Daun Binahong (Anredera Cordifiolia Steenis) Terhadap Pertumbuhan Streptococcus Mutans Secara In Vitro, Jurnal Ilmiah Farmasi – UNSRAT Vol. 4 No. 4 2302 – 2493
Robinson, T. (1995). Kandungan Organik Tumbuhan Tingkat Tinggi. Diterjemahkan oleh Padmawinata, K. Bandung, Indonesia : ITB Press.
Sulistyani S. Uji Efek Ekstrak Etanol 70% Daun Binahong (Anredera Cordifolia Steenis) Terhadap Kadar Alt ( Alanin Aminotransferase) pada Tikus Jantan Galur Wistar ( Rattus Norvegicus) yang Di Induksi dengan Parasetamol. Kedok Journal. 2013. h.3.
Saifudin, A., 2014, Senyawa alam metabolit sekunder teori, konsep dan teknik pemurnian Ed.1, Yogyakarta: Budi Utama. ISBN 978-602-280-472-7, 113 hal.
Strobel, G. A., Daisy, B. H., Castilo, U., Harper, J., 2004, Natural products from endophytic microorganisms, J Nat Prod., 67, 257-268.
Strobel, G., and Daisy, B., 2003, Bioprospecting for Microbial Endophytes and Their Natural Products, Microbiology and Molecular Biology Reviews., 67, 491-502.
Song, Hong-Chuan, Dan Qin, Mei-Jun Han, Ling Wang, Kou Zwang, dan Jin-Yan Dong.2017. Bioactive 2-Pyrone Metabolites From An Endophyc Phomopsis Asparagi SWUKJ%.2020 of Kadsura Angustifolia. Phytochemistry Letters 22, 235-40.
Shou-Jie LI, Xuang Zhang, Xiang-Hua Wang, dan Chang-Qi Zhao.2018. Novel natural compounds from endophytic fungi with anticancer actifity. European Journal of Medicinal Chemistry 156, 316-43.
Verma, V. C., Kharwan, R. N., Strobel, G. A., 2009, Chemical and fungsional diversity of natural products from plant associated endophytic fungi, Nat Prod Commun., 41, 511-654
Visagie CM, Houbraken J, Frisvad JC, et al. 2014. Identification and nomen- clature of the genus Penicillium. Studies in Mycology 78: 343–371.
Xu et al., 2009, Chromones from the endophytic fungus pestalotiopsis sp. Isolated from the Chinese mangrove plant Rhyzopora mucronata, J Nat Prod., 72, 662-665.
Xu, Lijian, Wei Meng, Cong Cao, Jiang Wang, Wenjun Shan, dan Qinggui Wang. 2015. Antibacterial and Antifungal Compounds from Marine Fungi. Marine Drugs 13(6), 3479-3513.
Zhao, J., T, Shan., Y, Mou., and L, Zhou., 2011, Plant-Derived Bioactive Compounds Produced by Endophytic Fungi, Mini Reviews in Medicinal Chemistry., 11, 159-168.
Zhang, H. W., Song, Y. C., and Tan, R.X., 2016, Biology and chemistry of endophytes,. 2001, 18, 448
.
Published
2019-12-31
How to Cite
Ola, A., Bria, E., & da Cunha, T. (2019). Analisis Kandungan Metabolit Sekunder dan Uji aktivitas Antibakteri Jamur Endofit Batang Binahong (Anredera COrdifiolia Steenis). Chemistry Notes, 1(2), 1-11. https://doi.org/10.35508/cn.v1i2.1197
Section
Articles
Copyright (c) 2019 Chemistry Notes
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
In order to publish in Chemistry Notes, authors are required to agree to the copyright permission stating that the authors give the publisher the right to reproduce, display or distribute the accepted manuscript. In this agreement the authors also automatically declare that the submitted manuscript is exempted from plagiarism issue and conflict of interest among the authors.
