Isolation and Identification of Halotolerant Bacteria in Saline Soils from the Coastal Areas of Oebelo White Sand Beach and Pariti Beach
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Yosep Lawa(1*)
Chemistry Education, Faculty of Teacher Training and Education, Nusa Cendana University, Adisucipto Street, Penfui, Kupang, NTT
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Serlina Utami(2)
Chemistry Education, Faculty of Teacher Training and Education, Nusa Cendana University, Adisucipto Street, Penfui, Kupang, NTT
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Rut Delviana Lede Kore(3)
Chemistry Education, Faculty of Teacher Training and Education, Nusa Cendana University, Adisucipto Street, Penfui, Kupang, NTT
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Yantus A. B. Neolaka(4)
Chemistry Education, Faculty of Teacher Training and Education, Nusa Cendana University, Adisucipto Street, Penfui, Kupang, NTT
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Arvinda Ceniorita Lalang(5)
Chemistry Education, Faculty of Teacher Training and Education, Nusa Cendana University, Adisucipto Street, Penfui, Kupang, NTT
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Jacky Anggara Nenohai(6)
Chemistry Education, Faculty of Teacher Training and Education, Nusa Cendana University, Adisucipto Street, Penfui, Kupang, NTT
https://orcid.org/0000-0002-3612-5337
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Maria Anastasia Kutu Woa(7)
Chemistry Education, Faculty of Teacher Training and Education, Nusa Cendana University, Adisucipto Street, Penfui, Kupang, NTT
(*) Corresponding Author
Abstract
A study has been conducted to isolate and identify halotolerant bacteria in saline soils from the coastal areas of Oebelo White Sand Beach and Pariti Beach. The sampling method used was random sampling. During sampling, pH, electrical conductivity (EC), and soil temperature were also measured. Bacterial isolation was performed using a serial dilution technique, resulting in three pure isolates from each beach sand sample. The isolates were cultured on Nutrient Agar (NA) medium and incubated at 37°C for 24 hours. The colonies obtained were small, round, and white. The isolates were then purified using the streak plate method. The halotolerant test was conducted using Tryptic Soy Agar (TSA) medium without added salt. The results showed that the bacterial colonies were halotolerant, as indicated by growth after 24 hours of incubation. Further biochemical tests, including motility, citrate utilization, urease, and sugar hydrolysis, revealed that all isolates from Oebelo White Sand samples showed positive results for all tests, whereas isolates from Pariti Beach exhibited some variations. These findings indicate that the isolates were capable of hydrolyzing urea into ammonia, utilizing citrate as a carbon and energy source, and fermenting sugars present in the medium.
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Copyright (c) 2025 Yosep Lawa, Serlina Utami, Rut Delviana Lede Kore, Yantus A. B. Neolaka, Arvinda Ceniorita Lalang, Jacky Anggara Nenohai, Maria Anastasia Kutu Woa
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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. Copyright is retained by the authors, and articles can be freely used and distributed by others.
